Restriction map for
pFN21AE2750
(Linear) (Six-base) MAPSORT of: KIEE2750.seq Check: 140 from: 1 to: 5802
KIEE2750.orf
With 182 enzymes: SgfI *
June 21, 2019 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1941 3657 5113 5802
Size: 1941 1716 1456 689
Fragments arranged by size:
1941 1716 1456 689
AccI GT'mk_AC
Cuts at: 0 2197 2798 3851 4649 5323 5802
Size: 2197 601 1053 798 674 479
Fragments arranged by size:
2197 1053 798 674 601 479
Acc65I G'GTAC_C
Cuts at: 0 5443 5802
Size: 5443 359
AclI AA'CG_TT
Cuts at: 0 1821 4788 5802
Size: 1821 2967 1014
Fragments arranged by size:
2967 1821 1014
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 1772 2576 2983 3133 3517 4162 5594
Size: 262 1510 804 407 150 384 645 1432
Cuts at: 5594 5802
Size: 208
Fragments arranged by size:
1510 1432 804 645 407 384 262 208
150
AfeI AGC'GCT
Cuts at: 0 264 5802
Size: 264 5538
AflIII A'CryG_T
Cuts at: 0 425 2462 2522 5802
Size: 425 2037 60 3280
Fragments arranged by size:
3280 2037 425 60
AgeI A'CCGG_T
Cuts at: 0 2662 5802
Size: 2662 3140
AhdI GACnn_n'nnGTC
Cuts at: 0 1468 4656 5802
Size: 1468 3188 1146
Fragments arranged by size:
3188 1468 1146
AleI CACnn'nnGTG
Cuts at: 0 970 2620 2644 2875 5802
Size: 970 1650 24 231 2927
Fragments arranged by size:
2927 1650 970 231 24
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 281 811 1378 5802
Size: 281 530 567 4424
Fragments arranged by size:
4424 567 530 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4773 4805 5802
Size: 4773 32 997
Fragments arranged by size:
4773 997 32
AlwNI CAG_nnn'CTG
Cuts at: 0 289 2299 3495 4032 4077 4246 5469 5802
Size: 289 2010 1196 537 45 169 1223 333
Fragments arranged by size:
2010 1223 1196 537 333 289 169 45
ApaI G_GGCC'C
Cuts at: 0 2602 3325 5668 5802
Size: 2602 723 2343 134
Fragments arranged by size:
2602 2343 723 134
ApoI r'AATT_y
Cuts at: 0 744 1417 3824 5802
Size: 744 673 2407 1978
Fragments arranged by size:
2407 1978 744 673
AvaI C'yCGr_G
Cuts at: 0 72 123 1755 2179 4662 5802
Size: 72 51 1632 424 2483 1140
Fragments arranged by size:
2483 1632 1140 424 72 51
BamHI G'GATC_C
Cuts at: 0 2539 4932 5802
Size: 2539 2393 870
Fragments arranged by size:
2539 2393 870
BanI G'GyrC_C
Cuts at: 0 14 1339 2307 2622 4485 4876 5334 5443
Size: 14 1325 968 315 1863 391 458 109
Cuts at: 5443 5802
Size: 359
Fragments arranged by size:
1863 1325 968 458 391 359 315 109
14
BanII G_rGCy'C
Cuts at: 0 194 236 1231 2602 2725 3325 3461 4335
Size: 194 42 995 1371 123 600 136 874
Cuts at: 4335 4766 4777 5505 5624 5668 5756 5802
Size: 431 11 728 119 44 88 46
Fragments arranged by size:
1371 995 874 728 600 431 194 136
123 119 88 46 44 42 11
BbeI G_GCGC'C
Cuts at: 0 18 5802
Size: 18 5784
BbsI GAAGACnn'nnnn_
Cuts at: 0 1790 2261 2396 3758 5261 5705 5802
Size: 1790 471 135 1362 1503 444 97
Fragments arranged by size:
1790 1503 1362 471 444 135 97
BbvCI CC'TCA_GC
Cuts at: 0 1286 2235 5348 5625 5802
Size: 1286 949 3113 277 177
Fragments arranged by size:
3113 1286 949 277 177
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1655 1689 5065 5099 5802
Size: 1655 34 3376 34 703
Fragments arranged by size:
3376 1655 703 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3165 3427 3479 4997 5802
Size: 3165 262 52 1518 805
Fragments arranged by size:
3165 1518 805 262 52
BclI T'GATC_A
Cuts at: 0 2513 2867 5802
Size: 2513 354 2935
Fragments arranged by size:
2935 2513 354
BglI GCCn_nnn'nGGC
Cuts at: 0 169 5092 5227 5556 5802
Size: 169 4923 135 329 246
Fragments arranged by size:
4923 329 246 169 135
BglII A'GATC_T
Cuts at: 0 3275 5802
Size: 3275 2527
BlpI GC'TnA_GC
Cuts at: 0 3598 4067 5504 5802
Size: 3598 469 1437 298
Fragments arranged by size:
3598 1437 469 298
Bme1580I G_kGCm'C
Cuts at: 0 260 2312 2602 3325 4631 4704 4748 5339
Size: 260 2052 290 723 1306 73 44 591
Cuts at: 5339 5668 5802
Size: 329 134
Fragments arranged by size:
2052 1306 723 591 329 290 260 134
73 44
BmgBI CAC'GTC
Cuts at: 0 1323 4176 5802
Size: 1323 2853 1626
Fragments arranged by size:
2853 1626 1323
BmrI ACTGGGnnnn_n'
Cuts at: 0 263 2051 3433 3916 4352 4442 4987 5007
Size: 263 1788 1382 483 436 90 545 20
Cuts at: 5007 5190 5802
Size: 183 612
Fragments arranged by size:
1788 1382 612 545 483 436 263 183
90 20
BmtI G_CTAG'C
Cuts at: 0 4436 5802
Size: 4436 1366
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3766 3887 4395 5802
Size: 3766 121 508 1407
Fragments arranged by size:
3766 1407 508 121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 914 1143 1443 2071 3532 3709 3778 5689
Size: 914 229 300 628 1461 177 69 1911
Cuts at: 5689 5802
Size: 113
Fragments arranged by size:
1911 1461 914 628 300 229 177 113
69
Bpu10I CC'TnA_GC
Cuts at: 0 1225 1286 2235 5348 5625 5802
Size: 1225 61 949 3113 277 177
Fragments arranged by size:
3113 1225 949 277 177 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1418 2092 2284 3285 3423 5802
Size: 1418 674 192 1001 138 2379
Fragments arranged by size:
2379 1418 1001 674 192 138
BsaI GGTCTCn'nnnn_
Cuts at: 0 924 1745 5802
Size: 924 821 4057
Fragments arranged by size:
4057 924 821
BsaAI yAC'GTr
Cuts at: 0 426 2463 4404 5802
Size: 426 2037 1941 1398
Fragments arranged by size:
2037 1941 1398 426
BsaBI GATnn'nnATC
Cuts at: 0 2861 3701 4711 4926 5802
Size: 2861 840 1010 215 876
Fragments arranged by size:
2861 1010 876 840 215
BsaHI Gr'CG_yC
Cuts at: 0 15 21 1594 1645 2708 3530 4297 4898
Size: 15 6 1573 51 1063 822 767 601
Cuts at: 4898 5078 5802
Size: 180 724
Fragments arranged by size:
1573 1063 822 767 724 601 180 51
15 6
BsaWI w'CCGG_w
Cuts at: 0 198 2662 3007 3705 3873 5802
Size: 198 2464 345 698 168 1929
Fragments arranged by size:
2464 1929 698 345 198 168
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 996 1026 1059 1089 4399 4429 5398 5428
Size: 996 30 33 30 3310 30 969 30
Cuts at: 5428 5802
Size: 374
Fragments arranged by size:
3310 996 969 374 33 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 949 1082 2735 3302 5056 5419 5709 5746
Size: 949 133 1653 567 1754 363 290 37
Cuts at: 5746 5764 5802
Size: 18 38
Fragments arranged by size:
1754 1653 949 567 363 290 133 38
37 18
BseYI C'CCAG_C
Cuts at: 0 302 403 3194 3590 3842 4509 4944 5027
Size: 302 101 2791 396 252 667 435 83
Cuts at: 5027 5098 5217 5673 5802
Size: 71 119 456 129
Fragments arranged by size:
2791 667 456 435 396 302 252 129
119 101 83 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 974 1254 2285 2917 2972 3292 3685
Size: 251 723 280 1031 632 55 320 393
Cuts at: 3685 3776 4975 5802
Size: 91 1199 827
Fragments arranged by size:
1199 1031 827 723 632 393 320 280
251 91 55
BsiEI CG_ry'CG
Cuts at: 0 87 169 1578 4518 5802
Size: 87 82 1409 2940 1284
Fragments arranged by size:
2940 1409 1284 87 82
BsiHKAI G_wGCw'C
Cuts at: 0 194 236 2451 2653 4335 4403 4589 4777
Size: 194 42 2215 202 1682 68 186 188
Cuts at: 4777 5505 5756 5782 5802
Size: 728 251 26 20
Fragments arranged by size:
2215 1682 728 251 202 194 188 186
68 42 26 20
BsiWI C'GTAC_G
Cuts at: 0 5564 5802
Size: 5564 238
BsmI GAATG_Cn'
Cuts at: 0 1057 1688 5802
Size: 1057 631 4114
Fragments arranged by size:
4114 1057 631
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1498 1943 3875 4166 5802
Size: 1498 445 1932 291 1636
Fragments arranged by size:
1932 1636 1498 445 291
Bsp1286I G_dGCh'C
Cuts at: 0 194 236 260 1231 2312 2451 2602 2653
Size: 194 42 24 971 1081 139 151 51
Cuts at: 2653 2725 3325 3461 4335 4403 4589 4631 4704
Size: 72 600 136 874 68 186 42 73
Cuts at: 4704 4748 4766 4777 5339 5505 5624 5668 5756
Size: 44 18 11 562 166 119 44 88
Cuts at: 5756 5782 5802
Size: 26 20
Fragments arranged by size:
1081 971 874 600 562 194 186 166
151 139 136 119 88 73 72 68
51 44 44 42 42 26 24 20
18 11
BspEI T'CCGG_A
Cuts at: 0 198 3873 5802
Size: 198 3675 1929
Fragments arranged by size:
3675 1929 198
BspHI T'CATG_A
Cuts at: 0 4113 5802
Size: 4113 1689
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1941 2537 3657 4310 5113 5802
Size: 1941 596 1120 653 803 689
Fragments arranged by size:
1941 1120 803 689 653 596
BsrBI CCG'CTC
Cuts at: 0 83 4343 5802
Size: 83 4260 1459
Fragments arranged by size:
4260 1459 83
BsrDI GCAATG_nn'
Cuts at: 0 1078 3836 5284 5802
Size: 1078 2758 1448 518
Fragments arranged by size:
2758 1448 1078 518
BsrFI r'CCGG_y
Cuts at: 0 168 1590 2662 4436 4850 5802
Size: 168 1422 1072 1774 414 952
Fragments arranged by size:
1774 1422 1072 952 414 168
BssHII G'CGCG_C
Cuts at: 0 4611 5802
Size: 4611 1191
BssSI C'ACGA_G
Cuts at: 0 174 235 1302 5089 5802
Size: 174 61 1067 3787 713
Fragments arranged by size:
3787 1067 713 174 61
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1054 2299 5802
Size: 1054 1245 3503
Fragments arranged by size:
3503 1245 1054
BstEII G'GTnAC_C
Cuts at: 0 3817 5677 5802
Size: 3817 1860 125
Fragments arranged by size:
3817 1860 125
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1256 2700 3813 5802
Size: 1256 1444 1113 1989
Fragments arranged by size:
1989 1444 1256 1113
BstYI r'GATC_y
Cuts at: 0 399 1428 1486 2539 3275 4037 4196 4412
Size: 399 1029 58 1053 736 762 159 216
Cuts at: 4412 4454 4601 4721 4932 5393 5459 5802
Size: 42 147 120 211 461 66 343
Fragments arranged by size:
1053 1029 762 736 461 399 343 216
211 159 147 120 66 58 42
BtgI C'CryG_G
Cuts at: 0 35 227 505 596 1490 1572 2152 2639
Size: 35 192 278 91 894 82 580 487
Cuts at: 2639 4623 4733 5693 5802
Size: 1984 110 960 109
Fragments arranged by size:
1984 960 894 580 487 278 192 110
109 91 82 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1025 4576 4886 5802
Size: 1025 3551 310 916
Fragments arranged by size:
3551 1025 916 310
BtsI GCAGTG_nn'
Cuts at: 0 1661 2297 2960 4126 4351 5802
Size: 1661 636 663 1166 225 1451
Fragments arranged by size:
1661 1451 1166 663 636 225
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 333 368 2052 2087 5802
Size: 333 35 1684 35 3715
Fragments arranged by size:
3715 1684 333 35 35
DraI TTT'AAA
Cuts at: 0 2111 5802
Size: 2111 3691
DraIII CAC_nnn'GTG
Cuts at: 0 1259 4689 5198 5802
Size: 1259 3430 509 604
Fragments arranged by size:
3430 1259 604 509
DrdI GACnn_nn'nnGTC
Cuts at: 0 4255 5802
Size: 4255 1547
EaeI y'GGCC_r
Cuts at: 0 32 84 166 449 1298 1575 2882 4007
Size: 32 52 82 283 849 277 1307 1125
Cuts at: 4007 4736 5228 5712 5802
Size: 729 492 484 90
Fragments arranged by size:
1307 1125 849 729 492 484 283 277
90 82 52 32
EagI C'GGCC_G
Cuts at: 0 84 166 1575 5802
Size: 84 82 1409 4227
Fragments arranged by size:
4227 1409 84 82
EarI CTCTTCn'nnn_
Cuts at: 0 1920 2057 2883 3856 4221 4259 4765 5174
Size: 1920 137 826 973 365 38 506 409
Cuts at: 5174 5583 5802
Size: 409 219
Fragments arranged by size:
1920 973 826 506 409 409 365 219
137 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 87 663 3636 4540 4594 5355 5802
Size: 87 576 2973 904 54 761 447
Fragments arranged by size:
2973 904 761 576 447 87 54
EcoICRI GAG'CTC
Cuts at: 0 192 234 4333 4775 5503 5754 5802
Size: 192 42 4099 442 728 251 48
Fragments arranged by size:
4099 728 442 251 192 48 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 914 1143 1443 1772 2071 2576 2983
Size: 262 652 229 300 329 299 505 407
Cuts at: 2983 3133 3517 3532 3709 3778 4162 5594 5689
Size: 150 384 15 177 69 384 1432 95
Cuts at: 5689 5802
Size: 113
Fragments arranged by size:
1432 652 505 407 384 384 329 300
299 262 229 177 150 113 95 69
15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 820 4884 5473 5802
Size: 820 4064 589 329
Fragments arranged by size:
4064 820 589 329
EcoO109I rG'GnC_Cy
Cuts at: 0 3321 4135 4322 4443 4889 5131 5204 5664
Size: 3321 814 187 121 446 242 73 460
Cuts at: 5664 5665 5802
Size: 1 137
Fragments arranged by size:
3321 814 460 446 242 187 137 121
73 1
EcoRI G'AATT_C
Cuts at: 0 3824 5802
Size: 3824 1978
EcoRV GAT'ATC
Cuts at: 0 2844 5802
Size: 2844 2958
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2183 4005 5802
Size: 2183 1822 1797
Fragments arranged by size:
2183 1822 1797
FseI GG_CCGG'CC
Cuts at: 0 172 5802
Size: 172 5630
HaeII r_GCGC'y
Cuts at: 0 18 266 4126 4503 4623 5802
Size: 18 248 3860 377 120 1179
Fragments arranged by size:
3860 1179 377 248 120 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 178 210 501 533 1059 1091 1117 1149
Size: 178 32 291 32 526 32 26 32
Cuts at: 1149 1312 1344 1454 1486 1852 1884 2020 2052
Size: 163 32 110 32 366 32 136 32
Cuts at: 2052 3791 3823 4234 4266 4306 4338 4399 4431
Size: 1739 32 411 32 40 32 61 32
Cuts at: 4431 4642 4645 4674 4677 5398 5430 5802
Size: 211 3 29 3 721 32 372
Fragments arranged by size:
1739 721 526 411 372 366 291 211
178 163 136 110 61 40 32 32
32 32 32 32 32 32 32 32
32 32 32 29 26 3 3
HincII GTy'rAC
Cuts at: 0 2799 3852 4311 4821 5802
Size: 2799 1053 459 510 981
Fragments arranged by size:
2799 1053 981 510 459
HindIII A'AGCT_T
Cuts at: 0 2160 3061 5802
Size: 2160 901 2741
Fragments arranged by size:
2741 2160 901
KasI G'GCGC_C
Cuts at: 0 14 5802
Size: 14 5788
KpnI G_GTAC'C
Cuts at: 0 5447 5802
Size: 5447 355
MluI A'CGCG_T
Cuts at: 0 2522 5802
Size: 2522 3280
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 754 3356 5802
Size: 754 2602 2446
Fragments arranged by size:
2602 2446 754
MscI TGG'CCA
Cuts at: 0 1300 2884 4009 5714 5802
Size: 1300 1584 1125 1705 88
Fragments arranged by size:
1705 1584 1300 1125 88
MslI CAynn'nnrTG
Cuts at: 0 412 970 1360 2119 2620 2644 2698 2875
Size: 412 558 390 759 501 24 54 177
Cuts at: 2875 3043 3922 5802
Size: 168 879 1880
Fragments arranged by size:
1880 879 759 558 501 412 390 177
168 54 24
MspA1I CmG'CkG
Cuts at: 0 104 658 1893 1904 2046 3594 4625 5802
Size: 104 554 1235 11 142 1548 1031 1177
Fragments arranged by size:
1548 1235 1177 1031 554 142 104 11
NaeI GCC'GGC
Cuts at: 0 170 1592 4438 5802
Size: 170 1422 2846 1364
Fragments arranged by size:
2846 1422 1364 170
NarI GG'CG_CC
Cuts at: 0 15 5802
Size: 15 5787
NcoI C'CATG_G
Cuts at: 0 35 227 596 1490 2152 5802
Size: 35 192 369 894 662 3650
Fragments arranged by size:
3650 894 662 369 192 35
NdeI CA'TA_TG
Cuts at: 0 663 2832 5802
Size: 663 2169 2970
Fragments arranged by size:
2970 2169 663
NgoMIV G'CCGG_C
Cuts at: 0 168 1590 4436 5802
Size: 168 1422 2846 1366
Fragments arranged by size:
2846 1422 1366 168
NheI G'CTAG_C
Cuts at: 0 4432 5802
Size: 4432 1370
PasI CC'CwG_GG
Cuts at: 0 557 5668 5802
Size: 557 5111 134
Fragments arranged by size:
5111 557 134
PflMI CCAn_nnn'nTGG
Cuts at: 0 736 5198 5302 5802
Size: 736 4462 104 500
Fragments arranged by size:
4462 736 500 104
PfoI T'CCnGG_A
Cuts at: 0 4724 5069 5635 5802
Size: 4724 345 566 167
Fragments arranged by size:
4724 566 345 167
PmlI CAC'GTG
Cuts at: 0 2463 4404 5802
Size: 2463 1941 1398
Fragments arranged by size:
2463 1941 1398
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 563 595 3112 3144 5802
Size: 563 32 2517 32 2658
Fragments arranged by size:
2658 2517 563 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 4135 4322 4443 5204 5802
Size: 4135 187 121 761 598
Fragments arranged by size:
4135 761 598 187 121
PshAI GACnn'nnGTC
Cuts at: 0 2374 5802
Size: 2374 3428
PsiI TTA'TAA
Cuts at: 0 3052 5802
Size: 3052 2750
PspOMI G'GGCC_C
Cuts at: 0 2598 3321 5664 5802
Size: 2598 723 2343 138
Fragments arranged by size:
2598 2343 723 138
PstI C_TGCA'G
Cuts at: 0 2036 3449 3651 4037 5245 5321 5663 5802
Size: 2036 1413 202 386 1208 76 342 139
Fragments arranged by size:
2036 1413 1208 386 342 202 139 76
PvuII CAG'CTG
Cuts at: 0 2046 3594 5802
Size: 2046 1548 2208
Fragments arranged by size:
2208 2046 1548
SacI G_AGCT'C
Cuts at: 0 194 236 4335 4777 5505 5756 5802
Size: 194 42 4099 442 728 251 46
Fragments arranged by size:
4099 728 442 251 194 46 42
SacII CC_GC'GG
Cuts at: 0 4626 5802
Size: 4626 1176
SalI G'TCGA_C
Cuts at: 0 2797 3850 5802
Size: 2797 1053 1952
Fragments arranged by size:
2797 1952 1053
SanDI GG'GwC_CC
Cuts at: 0 4443 5802
Size: 4443 1359
SapI GCTCTTCn'nnn_
Cuts at: 0 2883 4765 5802
Size: 2883 1882 1037
Fragments arranged by size:
2883 1882 1037
ScaI AGT'ACT
Cuts at: 0 2930 3146 5802
Size: 2930 216 2656
Fragments arranged by size:
2930 2656 216
SexAI A'CCwGG_T
Cuts at: 0 4814 5295 5802
Size: 4814 481 507
Fragments arranged by size:
4814 507 481
SfcI C'TryA_G
Cuts at: 0 2032 3445 3641 3647 4033 4881 5211 5241
Size: 2032 1413 196 6 386 848 330 30
Cuts at: 5241 5317 5324 5659 5802
Size: 76 7 335 143
Fragments arranged by size:
2032 1413 848 386 335 330 196 143
76 30 7 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 169 5556 5802
Size: 169 5387 246
Fragments arranged by size:
5387 246 169
SfoI GGC'GCC
Cuts at: 0 16 5802
Size: 16 5786
SgrAI Cr'CCGG_yG
Cuts at: 0 2662 5802
Size: 2662 3140
SmaI CCC'GGG
Cuts at: 0 74 125 5802
Size: 74 51 5677
Fragments arranged by size:
5677 74 51
SmlI C'TyrA_G
Cuts at: 0 1433 2071 2179 2263 3300 3402 5802
Size: 1433 638 108 84 1037 102 2400
Fragments arranged by size:
2400 1433 1037 638 108 102 84
StuI AGG'CCT
Cuts at: 0 3212 5362 5802
Size: 3212 2150 440
Fragments arranged by size:
3212 2150 440
StyI C'CwwG_G
Cuts at: 0 35 227 372 596 908 1490 1994 2152
Size: 35 192 145 224 312 582 504 158
Cuts at: 2152 5602 5802
Size: 3450 200
Fragments arranged by size:
3450 582 504 312 224 200 192 158
145 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 686 1665 2628 2692 3208 4532 5209 5802
Size: 686 979 963 64 516 1324 677 593
Fragments arranged by size:
1324 979 963 686 677 593 516 64
TatI w'GTAC_w
Cuts at: 0 391 2903 2928 3144 5015 5802
Size: 391 2512 25 216 1871 787
Fragments arranged by size:
2512 1871 787 391 216 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1545 1577 2664 2696 3425 3457 4478 4510
Size: 1545 32 1087 32 729 32 1021 32
Cuts at: 4510 5802
Size: 1292
Fragments arranged by size:
1545 1292 1087 1021 729 32 32 32
32
XbaI T'CTAG_A
Cuts at: 0 1483 2451 5802
Size: 1483 968 3351
Fragments arranged by size:
3351 1483 968
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 411 491 507 938 1308 5802
Size: 411 80 16 431 370 4494
Fragments arranged by size:
4494 431 411 370 80 16
XhoI C'TCGA_G
Cuts at: 0 2179 5802
Size: 2179 3623
XmaI C'CCGG_G
Cuts at: 0 72 123 5802
Size: 72 51 5679
Fragments arranged by size:
5679 72 51
XmnI GAAnn'nnTTC
Cuts at: 0 3560 4054 4230 5802
Size: 3560 494 176 1572
Fragments arranged by size:
3560 1572 494 176
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FseI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI PasI PflMI PfoI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuII SacI SacII
SalI SanDI SapI ScaI SexAI SfcI SfiI SfoI
SgrAI SmaI SmlI StuI StyI TaqII TatI TstI
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII ApaLI AscI AseI AsiSI AvrII BaeI
BfrBI BsrGI BstBI BstZ17I Bsu36I ClaI FspI FspAI
HpaI MfeI NotI NruI NsiI NspI PacI PciI
PmeI PspXI PsrI PvuI RsrII SbfI SgfI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I ZraI