Restriction map for
pFN21AE2749
(Linear) (Six-base) MAPSORT of: KIEE2749.seq Check: 9450 from: 1 to: 639
KIEE2749.orf
With 182 enzymes: SgfI *
June 19, 2019 14:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 639
Size: 46 593
ApaI G_GGCC'C
Cuts at: 0 413 639
Size: 413 226
AvaI C'yCGr_G
Cuts at: 0 70 199 363 639
Size: 70 129 164 276
Fragments arranged by size:
276 164 129 70
BanI G'GyrC_C
Cuts at: 0 193 639
Size: 193 446
BanII G_rGCy'C
Cuts at: 0 413 617 639
Size: 413 204 22
Fragments arranged by size:
413 204 22
BbeI G_GCGC'C
Cuts at: 0 197 639
Size: 197 442
BlpI GC'TnA_GC
Cuts at: 0 631 639
Size: 631 8
Bme1580I G_kGCm'C
Cuts at: 0 305 413 494 639
Size: 305 108 81 145
Fragments arranged by size:
305 145 108 81
BmrI ACTGGGnnnn_n'
Cuts at: 0 612 639
Size: 612 27
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 369 421 639
Size: 369 52 218
Fragments arranged by size:
369 218 52
BsaAI yAC'GTr
Cuts at: 0 417 639
Size: 417 222
BsaHI Gr'CG_yC
Cuts at: 0 194 554 639
Size: 194 360 85
Fragments arranged by size:
360 194 85
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 277 307 639
Size: 277 30 332
Fragments arranged by size:
332 277 30
BseYI C'CCAG_C
Cuts at: 0 405 493 639
Size: 405 88 146
Fragments arranged by size:
405 146 88
BsiHKAI G_wGCw'C
Cuts at: 0 326 355 565 639
Size: 326 29 210 74
Fragments arranged by size:
326 210 74 29
Bsp1286I G_dGCh'C
Cuts at: 0 305 326 355 413 494 565 617 639
Size: 305 21 29 58 81 71 52 22
Fragments arranged by size:
305 81 71 58 52 29 22 21
BstAPI GCAn_nnn'nTGC
Cuts at: 0 359 639
Size: 359 280
BstXI CCAn_nnnn'nTGG
Cuts at: 0 276 639
Size: 276 363
BtgI C'CryG_G
Cuts at: 0 105 639
Size: 105 534
BtsI GCAGTG_nn'
Cuts at: 0 494 516 639
Size: 494 22 123
Fragments arranged by size:
494 123 22
EaeI y'GGCC_r
Cuts at: 0 62 236 266 482 567 639
Size: 62 174 30 216 85 72
Fragments arranged by size:
216 174 85 72 62 30
EarI CTCTTCn'nnn_
Cuts at: 0 22 639
Size: 22 617
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 369 421 639
Size: 46 323 52 218
Fragments arranged by size:
323 218 52 46
EcoO109I rG'GnC_Cy
Cuts at: 0 409 410 438 639
Size: 409 1 28 201
Fragments arranged by size:
409 201 28 1
HaeII r_GCGC'y
Cuts at: 0 197 348 639
Size: 197 151 291
Fragments arranged by size:
291 197 151
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 604 636 639
Size: 604 32 3
Fragments arranged by size:
604 32 3
KasI G'GCGC_C
Cuts at: 0 193 639
Size: 193 446
MscI TGG'CCA
Cuts at: 0 64 268 569 639
Size: 64 204 301 70
Fragments arranged by size:
301 204 70 64
MslI CAynn'nnrTG
Cuts at: 0 232 319 639
Size: 232 87 320
Fragments arranged by size:
320 232 87
MspA1I CmG'CkG
Cuts at: 0 168 359 539 639
Size: 168 191 180 100
Fragments arranged by size:
191 180 168 100
NarI GG'CG_CC
Cuts at: 0 194 639
Size: 194 445
PflMI CCAn_nnn'nTGG
Cuts at: 0 111 639
Size: 111 528
PspOMI G'GGCC_C
Cuts at: 0 409 639
Size: 409 230
PstI C_TGCA'G
Cuts at: 0 58 639
Size: 58 581
PvuII CAG'CTG
Cuts at: 0 539 639
Size: 539 100
SexAI A'CCwGG_T
Cuts at: 0 425 639
Size: 425 214
SfcI C'TryA_G
Cuts at: 0 54 120 528 639
Size: 54 66 408 111
Fragments arranged by size:
408 111 66 54
SfoI GGC'GCC
Cuts at: 0 195 639
Size: 195 444
StuI AGG'CCT
Cuts at: 0 76 639
Size: 76 563
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 396 639
Size: 396 243
Enzymes that do cut:
AcuI ApaI AvaI BanI BanII BbeI BlpI Bme1580I
BmrI BpmI BsaAI BsaHI BsaXI BseYI BsiHKAI Bsp1286I
BstAPI BstXI BtgI BtsI EaeI EarI Eco57MI EcoO109I
HaeII Hin4I KasI MscI MslI MspA1I NarI PflMI
PspOMI PstI PvuII SexAI SfcI SfoI StuI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BmgBI BmtI
BplI Bpu10I BpuEI BsaI BsaBI BsaWI BseRI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StyI SwaI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI