Restriction map for
pFN21AE2737
(Linear) (Six-base) MAPSORT of: KIEE2737.seq Check: 3530 from: 1 to: 843
KIEE2737.orf
With 182 enzymes: SgfI *
December 20, 2018 16:25 ..
AatII G_ACGT'C
Cuts at: 0 365 843
Size: 365 478
AccI GT'mk_AC
Cuts at: 0 818 843
Size: 818 25
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 663 843
Size: 663 180
ApaI G_GGCC'C
Cuts at: 0 237 339 843
Size: 237 102 504
Fragments arranged by size:
504 237 102
AvaI C'yCGr_G
Cuts at: 0 400 763 769 843
Size: 400 363 6 74
Fragments arranged by size:
400 363 74 6
BamHI G'GATC_C
Cuts at: 0 104 843
Size: 104 739
BanI G'GyrC_C
Cuts at: 0 391 843
Size: 391 452
BanII G_rGCy'C
Cuts at: 0 237 248 339 770 843
Size: 237 11 91 431 73
Fragments arranged by size:
431 237 91 73 11
BbvCI CC'TCA_GC
Cuts at: 0 22 843
Size: 22 821
BglI GCCn_nnn'nGGC
Cuts at: 0 260 843
Size: 260 583
BglII A'GATC_T
Cuts at: 0 164 843
Size: 164 679
BlpI GC'TnA_GC
Cuts at: 0 601 843
Size: 601 242
Bme1580I G_kGCm'C
Cuts at: 0 237 339 533 843
Size: 237 102 194 310
Fragments arranged by size:
310 237 194 102
BmgBI CAC'GTC
Cuts at: 0 816 843
Size: 816 27
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 685 843
Size: 364 321 158
Fragments arranged by size:
364 321 158
Bpu10I CC'TnA_GC
Cuts at: 0 22 843
Size: 22 821
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 843
Size: 223 620
BsaI GGTCTCn'nnnn_
Cuts at: 0 400 843
Size: 400 443
BsaHI Gr'CG_yC
Cuts at: 0 362 752 843
Size: 362 390 91
Fragments arranged by size:
390 362 91
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 418 433 843
Size: 418 15 410
Fragments arranged by size:
418 410 15
BseYI C'CCAG_C
Cuts at: 0 338 425 596 843
Size: 338 87 171 247
Fragments arranged by size:
338 247 171 87
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 659 697 819 843
Size: 457 202 38 122 24
Fragments arranged by size:
457 202 122 38 24
BsiEI CG_ry'CG
Cuts at: 0 416 843
Size: 416 427
BsiHKAI G_wGCw'C
Cuts at: 0 248 770 843
Size: 248 522 73
Fragments arranged by size:
522 248 73
Bsp1286I G_dGCh'C
Cuts at: 0 237 248 339 533 770 843
Size: 237 11 91 194 237 73
Fragments arranged by size:
237 237 194 91 73 11
BspHI T'CATG_A
Cuts at: 0 776 843
Size: 776 67
BsrFI r'CCGG_y
Cuts at: 0 824 843
Size: 824 19
BssHII G'CGCG_C
Cuts at: 0 689 843
Size: 689 154
BstAPI GCAn_nnn'nTGC
Cuts at: 0 528 843
Size: 528 315
BstXI CCAn_nnnn'nTGG
Cuts at: 0 180 843
Size: 180 663
BstYI r'GATC_y
Cuts at: 0 104 164 843
Size: 104 60 679
Fragments arranged by size:
679 104 60
BtgI C'CryG_G
Cuts at: 0 203 410 843
Size: 203 207 433
Fragments arranged by size:
433 207 203
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 195 843
Size: 195 648
DraIII CAC_nnn'GTG
Cuts at: 0 528 843
Size: 528 315
EaeI y'GGCC_r
Cuts at: 0 182 413 843
Size: 182 231 430
Fragments arranged by size:
430 231 182
EagI C'GGCC_G
Cuts at: 0 413 843
Size: 413 430
EarI CTCTTCn'nnn_
Cuts at: 0 49 64 843
Size: 49 15 779
Fragments arranged by size:
779 49 15
EcoICRI GAG'CTC
Cuts at: 0 246 768 843
Size: 246 522 75
Fragments arranged by size:
522 246 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 685 843
Size: 364 321 158
Fragments arranged by size:
364 321 158
EcoNI CCTnn'n_nnAGG
Cuts at: 0 580 843
Size: 580 263
EcoO109I rG'GnC_Cy
Cuts at: 0 233 234 696 843
Size: 233 1 462 147
Fragments arranged by size:
462 233 147 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 141 606 843
Size: 141 465 237
Fragments arranged by size:
465 237 141
HindIII A'AGCT_T
Cuts at: 0 351 843
Size: 351 492
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 494 843
Size: 494 349
MscI TGG'CCA
Cuts at: 0 184 843
Size: 184 659
MspA1I CmG'CkG
Cuts at: 0 251 333 342 412 447 843
Size: 251 82 9 70 35 396
Fragments arranged by size:
396 251 82 70 35 9
NcoI C'CATG_G
Cuts at: 0 203 843
Size: 203 640
PasI CC'CwG_GG
Cuts at: 0 309 843
Size: 309 534
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 757 789 843
Size: 757 32 54
Fragments arranged by size:
757 54 32
PspOMI G'GGCC_C
Cuts at: 0 233 335 843
Size: 233 102 508
Fragments arranged by size:
508 233 102
PspXI vC'TCGA_Gb
Cuts at: 0 763 843
Size: 763 80
PstI C_TGCA'G
Cuts at: 0 164 663 843
Size: 164 499 180
Fragments arranged by size:
499 180 164
PvuII CAG'CTG
Cuts at: 0 342 447 843
Size: 342 105 396
Fragments arranged by size:
396 342 105
SacI G_AGCT'C
Cuts at: 0 248 770 843
Size: 248 522 73
Fragments arranged by size:
522 248 73
SacII CC_GC'GG
Cuts at: 0 413 843
Size: 413 430
ScaI AGT'ACT
Cuts at: 0 37 843
Size: 37 806
SfcI C'TryA_G
Cuts at: 0 160 659 843
Size: 160 499 184
Fragments arranged by size:
499 184 160
SmlI C'TyrA_G
Cuts at: 0 238 763 843
Size: 238 525 80
Fragments arranged by size:
525 238 80
StyI C'CwwG_G
Cuts at: 0 203 843
Size: 203 640
TatI w'GTAC_w
Cuts at: 0 35 843
Size: 35 808
Tth111I GACn'n_nGTC
Cuts at: 0 814 843
Size: 814 29
XhoI C'TCGA_G
Cuts at: 0 763 843
Size: 763 80
ZraI GAC'GTC
Cuts at: 0 363 843
Size: 363 480
Enzymes that do cut:
AatII AccI AlfI ApaI AvaI BamHI BanI BanII
BbvCI BglI BglII BlpI Bme1580I BmgBI BpmI Bpu10I
BpuEI BsaI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BsrFI BssHII BstAPI BstXI BstYI BtgI
BtgZI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I FalI HindIII MmeI MscI MspA1I NcoI PasI
PpiI PspOMI PspXI PstI PvuII SacI SacII ScaI
SfcI SmlI StyI TatI Tth111I XhoI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbeI BbsI BcgI BciVI BclI
BfrBI BmrI BmtI BplI BsaAI BsaBI BsaWI BsaXI
BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI BsrGI
BssSI BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI
DraI DrdI EciI EcoRI EcoRV FseI FspI FspAI
HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI
MslI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PflMI PfoI PmeI PmlI
PpuMI PshAI PsiI PsrI PvuI RsrII SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TstI XbaI XcmI XmaI XmnI