Restriction map for
pFN21AE2733
(Linear) (Six-base) MAPSORT of: KIEE2733.seq Check: 3681 from: 1 to: 1044
KIEE2733.orf
With 182 enzymes: SgfI *
September 11, 2018 18:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 605 1044
Size: 605 439
AccI GT'mk_AC
Cuts at: 0 587 1044
Size: 587 457
Acc65I G'GTAC_C
Cuts at: 0 184 1044
Size: 184 860
AhdI GACnn_n'nnGTC
Cuts at: 0 957 1044
Size: 957 87
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 59 1044
Size: 59 985
AlwNI CAG_nnn'CTG
Cuts at: 0 145 171 690 830 856 1044
Size: 145 26 519 140 26 188
Fragments arranged by size:
519 188 145 140 26 26
ApaI G_GGCC'C
Cuts at: 0 27 1044
Size: 27 1017
ApoI r'AATT_y
Cuts at: 0 134 1044
Size: 134 910
AvaI C'yCGr_G
Cuts at: 0 81 353 458 510 805 1044
Size: 81 272 105 52 295 239
Fragments arranged by size:
295 272 239 105 81 52
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 719 752 1044
Size: 719 33 292
Fragments arranged by size:
719 292 33
BanI G'GyrC_C
Cuts at: 0 184 793 1044
Size: 184 609 251
Fragments arranged by size:
609 251 184
BanII G_rGCy'C
Cuts at: 0 27 332 473 935 1044
Size: 27 305 141 462 109
Fragments arranged by size:
462 305 141 109 27
BglI GCCn_nnn'nGGC
Cuts at: 0 892 1024 1044
Size: 892 132 20
Fragments arranged by size:
892 132 20
Bme1580I G_kGCm'C
Cuts at: 0 27 1044
Size: 27 1017
BmgBI CAC'GTC
Cuts at: 0 735 843 1044
Size: 735 108 201
Fragments arranged by size:
735 201 108
BmrI ACTGGGnnnn_n'
Cuts at: 0 181 250 395 1044
Size: 181 69 145 649
Fragments arranged by size:
649 181 145 69
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 405 432 976 1044
Size: 405 27 544 68
Fragments arranged by size:
544 405 68 27
Bpu10I CC'TnA_GC
Cuts at: 0 517 834 1044
Size: 517 317 210
Fragments arranged by size:
517 317 210
BsaI GGTCTCn'nnnn_
Cuts at: 0 93 490 1044
Size: 93 397 554
Fragments arranged by size:
554 397 93
BsaHI Gr'CG_yC
Cuts at: 0 269 1044
Size: 269 775
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 168 198 521 551 1044
Size: 168 30 323 30 493
Fragments arranged by size:
493 323 168 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 481 547 630 1044
Size: 481 66 83 414
Fragments arranged by size:
481 414 83 66
BseYI C'CCAG_C
Cuts at: 0 200 250 786 849 877 1044
Size: 200 50 536 63 28 167
Fragments arranged by size:
536 200 167 63 50 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 472 634 925 1044
Size: 229 243 162 291 119
Fragments arranged by size:
291 243 229 162 119
BsiHKAI G_wGCw'C
Cuts at: 0 332 421 977 1044
Size: 332 89 556 67
Fragments arranged by size:
556 332 89 67
BsmBI CGTCTCn'nnnn_
Cuts at: 0 276 833 1044
Size: 276 557 211
Fragments arranged by size:
557 276 211
Bsp1286I G_dGCh'C
Cuts at: 0 27 332 421 473 935 977 1044
Size: 27 305 89 52 462 42 67
Fragments arranged by size:
462 305 89 67 52 42 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 605 1044
Size: 605 439
BsrBI CCG'CTC
Cuts at: 0 387 504 937 1044
Size: 387 117 433 107
Fragments arranged by size:
433 387 117 107
BsrFI r'CCGG_y
Cuts at: 0 565 600 790 1044
Size: 565 35 190 254
Fragments arranged by size:
565 254 190 35
BsrGI T'GTAC_A
Cuts at: 0 11 1044
Size: 11 1033
BssSI C'ACGA_G
Cuts at: 0 928 1044
Size: 928 116
BstAPI GCAn_nnn'nTGC
Cuts at: 0 830 1044
Size: 830 214
BstXI CCAn_nnnn'nTGG
Cuts at: 0 110 1044
Size: 110 934
BstYI r'GATC_y
Cuts at: 0 395 826 1044
Size: 395 431 218
Fragments arranged by size:
431 395 218
Bsu36I CC'TnA_GG
Cuts at: 0 674 1044
Size: 674 370
BtgI C'CryG_G
Cuts at: 0 491 1044
Size: 491 553
BtsI GCAGTG_nn'
Cuts at: 0 750 917 1003 1044
Size: 750 167 86 41
Fragments arranged by size:
750 167 86 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 596 1044
Size: 596 448
EcoICRI GAG'CTC
Cuts at: 0 330 1044
Size: 330 714
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 405 432 976 1044
Size: 405 27 544 68
Fragments arranged by size:
544 405 68 27
EcoO109I rG'GnC_Cy
Cuts at: 0 23 24 142 407 670 1044
Size: 23 1 118 265 263 374
Fragments arranged by size:
374 265 263 118 23 1
HaeII r_GCGC'y
Cuts at: 0 777 1044
Size: 777 267
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 943 975 1044
Size: 943 32 69
Fragments arranged by size:
943 69 32
KpnI G_GTAC'C
Cuts at: 0 188 1044
Size: 188 856
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 1044
Size: 76 968
MslI CAynn'nnrTG
Cuts at: 0 947 1044
Size: 947 97
MspA1I CmG'CkG
Cuts at: 0 425 624 760 1044
Size: 425 199 136 284
Fragments arranged by size:
425 284 199 136
NaeI GCC'GGC
Cuts at: 0 567 602 792 1044
Size: 567 35 190 252
Fragments arranged by size:
567 252 190 35
NgoMIV G'CCGG_C
Cuts at: 0 565 600 790 1044
Size: 565 35 190 254
Fragments arranged by size:
565 254 190 35
PasI CC'CwG_GG
Cuts at: 0 898 1044
Size: 898 146
PflMI CCAn_nnn'nTGG
Cuts at: 0 1014 1044
Size: 1014 30
PfoI T'CCnGG_A
Cuts at: 0 389 443 1044
Size: 389 54 601
Fragments arranged by size:
601 389 54
PpuMI rG'GwC_Cy
Cuts at: 0 407 670 1044
Size: 407 263 374
Fragments arranged by size:
407 374 263
PshAI GACnn'nnGTC
Cuts at: 0 583 1044
Size: 583 461
PspOMI G'GGCC_C
Cuts at: 0 23 1044
Size: 23 1021
PstI C_TGCA'G
Cuts at: 0 434 618 826 998 1044
Size: 434 184 208 172 46
Fragments arranged by size:
434 208 184 172 46
PvuII CAG'CTG
Cuts at: 0 425 1044
Size: 425 619
SacI G_AGCT'C
Cuts at: 0 332 1044
Size: 332 712
SbfI CC_TGCA'GG
Cuts at: 0 434 1044
Size: 434 610
SfcI C'TryA_G
Cuts at: 0 430 614 822 994 1044
Size: 430 184 208 172 50
Fragments arranged by size:
430 208 184 172 50
SmaI CCC'GGG
Cuts at: 0 83 460 512 807 1044
Size: 83 377 52 295 237
Fragments arranged by size:
377 295 237 83 52
StyI C'CwwG_G
Cuts at: 0 908 1044
Size: 908 136
TatI w'GTAC_w
Cuts at: 0 11 745 1044
Size: 11 734 299
Fragments arranged by size:
734 299 11
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1011 1044
Size: 1011 33
XmaI C'CCGG_G
Cuts at: 0 81 458 510 805 1044
Size: 81 377 52 295 239
Fragments arranged by size:
377 295 239 81 52
Enzymes that do cut:
AarI AccI Acc65I AhdI AlfI AlwNI ApaI ApoI
AvaI BaeI BanI BanII BglI Bme1580I BmgBI BmrI
BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtsI EciI EcoICRI
Eco57MI EcoO109I HaeII Hin4I KpnI MmeI MslI MspA1I
NaeI NgoMIV PasI PflMI PfoI PpuMI PshAI PspOMI
PstI PvuII SacI SbfI SfcI SmaI StyI TatI
XcmI XmaI
Enzymes that do not cut:
AatII AclI AcuI AfeI AflII AflIII AgeI AleI
AloI ApaLI AscI AseI AsiSI AvrII BamHI BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BmtI BplI BpuEI BsaAI BsaBI BsaWI BsiEI BsiWI
BsmI BspEI BspHI BsrDI BssHII BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI MfeI MluI MscI NarI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PciI PmeI PmlI PpiI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TstI Tth111I XbaI XhoI
XmnI ZraI