Restriction map for
pFN21AE2732
(Linear) (Six-base) MAPSORT of: KIEE2732.seq Check: 3651 from: 1 to: 4824
KIEE2732.orf
With 182 enzymes: SgfI *
October 2, 2018 18:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2811 4084 4824
Size: 2811 1273 740
Fragments arranged by size:
2811 1273 740
AccI GT'mk_AC
Cuts at: 0 908 1554 1589 4088 4824
Size: 908 646 35 2499 736
Fragments arranged by size:
2499 908 736 646 35
Acc65I G'GTAC_C
Cuts at: 0 244 1410 3459 3923 4824
Size: 244 1166 2049 464 901
Fragments arranged by size:
2049 1166 901 464 244
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 944 1105 1621 1831 1912 1996 2422 2989
Size: 944 161 516 210 81 84 426 567
Cuts at: 2989 4288 4553 4736 4824
Size: 1299 265 183 88
Fragments arranged by size:
1299 944 567 516 426 265 210 183
161 88 84 81
AflIII A'CryG_T
Cuts at: 0 3029 4311 4824
Size: 3029 1282 513
Fragments arranged by size:
3029 1282 513
AhdI GACnn_n'nnGTC
Cuts at: 0 650 1452 2933 4824
Size: 650 802 1481 1891
Fragments arranged by size:
1891 1481 802 650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1931 4824
Size: 1931 2893
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1829 1861 3016 3048 4824
Size: 1829 32 1155 32 1776
Fragments arranged by size:
1829 1776 1155 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 601 2967 4824
Size: 601 2366 1857
Fragments arranged by size:
2366 1857 601
ApoI r'AATT_y
Cuts at: 0 1251 1714 2190 2299 2326 2491 2561 3043
Size: 1251 463 476 109 27 165 70 482
Cuts at: 3043 4824
Size: 1781
Fragments arranged by size:
1781 1251 482 476 463 165 109 70
27
AseI AT'TA_AT
Cuts at: 0 884 4426 4824
Size: 884 3542 398
Fragments arranged by size:
3542 884 398
AvaI C'yCGr_G
Cuts at: 0 112 130 4824
Size: 112 18 4694
Fragments arranged by size:
4694 112 18
AvrII C'CTAG_G
Cuts at: 0 1405 4824
Size: 1405 3419
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 947 980 4824
Size: 947 33 3844
Fragments arranged by size:
3844 947 33
BamHI G'GATC_C
Cuts at: 0 2123 4824
Size: 2123 2701
BanI G'GyrC_C
Cuts at: 0 244 1410 2752 3459 3923 4396 4527 4824
Size: 244 1166 1342 707 464 473 131 297
Fragments arranged by size:
1342 1166 707 473 464 297 244 131
BanII G_rGCy'C
Cuts at: 0 2400 2411 2730 4229 4824
Size: 2400 11 319 1499 595
Fragments arranged by size:
2400 1499 595 319 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 212 4824
Size: 212 4612
BbvCI CC'TCA_GC
Cuts at: 0 565 2609 3053 4824
Size: 565 2044 444 1771
Fragments arranged by size:
2044 1771 565 444
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2704 2738 4068 4102 4824
Size: 2704 34 1330 34 722
Fragments arranged by size:
2704 1330 722 34 34
BclI T'GATC_A
Cuts at: 0 465 4824
Size: 465 4359
BfrBI ATG'CAT
Cuts at: 0 2980 3374 4824
Size: 2980 394 1450
Fragments arranged by size:
2980 1450 394
BlpI GC'TnA_GC
Cuts at: 0 587 4824
Size: 587 4237
Bme1580I G_kGCm'C
Cuts at: 0 163 608 1234 1671 2755 3663 4824
Size: 163 445 626 437 1084 908 1161
Fragments arranged by size:
1161 1084 908 626 445 437 163
BmrI ACTGGGnnnn_n'
Cuts at: 0 2503 4444 4824
Size: 2503 1941 380
Fragments arranged by size:
2503 1941 380
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1551 1940 2050 3494 4605 4824
Size: 1551 389 110 1444 1111 219
Fragments arranged by size:
1551 1444 1111 389 219 110
Bpu10I CC'TnA_GC
Cuts at: 0 565 2609 3053 4824
Size: 565 2044 444 1771
Fragments arranged by size:
2044 1771 565 444
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1501 1956 2132 3263 3366 3735 4026 4765
Size: 1501 455 176 1131 103 369 291 739
Cuts at: 4765 4824
Size: 59
Fragments arranged by size:
1501 1131 739 455 369 291 176 103
59
BsaI GGTCTCn'nnnn_
Cuts at: 0 109 1050 1905 2510 4824
Size: 109 941 855 605 2314
Fragments arranged by size:
2314 941 855 605 109
BsaAI yAC'GTr
Cuts at: 0 1672 4824
Size: 1672 3152
BsaBI GATnn'nnATC
Cuts at: 0 545 2696 4824
Size: 545 2151 2128
Fragments arranged by size:
2151 2128 545
BsaWI w'CCGG_w
Cuts at: 0 105 1413 4824
Size: 105 1308 3411
Fragments arranged by size:
3411 1308 105
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 20 82 1792 1819 2240 2626 3778 3806
Size: 20 62 1710 27 421 386 1152 28
Cuts at: 3806 3951 4774 4824
Size: 145 823 50
Fragments arranged by size:
1710 1152 823 421 386 145 62 50
28 27 20
BseYI C'CCAG_C
Cuts at: 0 256 1514 2747 3613 4296 4824
Size: 256 1258 1233 866 683 528
Fragments arranged by size:
1258 1233 866 683 528 256
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 611 1189 1768 4824
Size: 611 578 579 3056
Fragments arranged by size:
3056 611 579 578
BsiEI CG_ry'CG
Cuts at: 0 1695 4577 4824
Size: 1695 2882 247
Fragments arranged by size:
2882 1695 247
BsiHKAI G_wGCw'C
Cuts at: 0 490 1524 1537 2411 4229 4649 4824
Size: 490 1034 13 874 1818 420 175
Fragments arranged by size:
1818 1034 874 490 420 175 13
BsmI GAATG_Cn'
Cuts at: 0 357 2273 3376 4824
Size: 357 1916 1103 1448
Fragments arranged by size:
1916 1448 1103 357
Bsp1286I G_dGCh'C
Cuts at: 0 163 490 608 1234 1524 1537 1671 2400
Size: 163 327 118 626 290 13 134 729
Cuts at: 2400 2411 2730 2755 3663 4229 4649 4824
Size: 11 319 25 908 566 420 175
Fragments arranged by size:
908 729 626 566 420 327 319 290
175 163 134 118 25 13 11
BspHI T'CATG_A
Cuts at: 0 1020 4824
Size: 1020 3804
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 633 1047 1848 1923 2811 3318 4084 4824
Size: 633 414 801 75 888 507 766 740
Fragments arranged by size:
888 801 766 740 633 507 414 75
BsrDI GCAATG_nn'
Cuts at: 0 588 1108 2258 3012 3110 4343 4824
Size: 588 520 1150 754 98 1233 481
Fragments arranged by size:
1233 1150 754 588 520 481 98
BsrFI r'CCGG_y
Cuts at: 0 2940 4824
Size: 2940 1884
BsrGI T'GTAC_A
Cuts at: 0 480 4070 4244 4824
Size: 480 3590 174 580
Fragments arranged by size:
3590 580 480 174
BssSI C'ACGA_G
Cuts at: 0 4544 4824
Size: 4544 280
BstBI TT'CG_AA
Cuts at: 0 1835 4181 4824
Size: 1835 2346 643
Fragments arranged by size:
2346 1835 643
BstEII G'GTnAC_C
Cuts at: 0 4014 4824
Size: 4014 810
BstXI CCAn_nnnn'nTGG
Cuts at: 0 4369 4824
Size: 4369 455
BstYI r'GATC_y
Cuts at: 0 434 514 546 1722 2076 2123 4824
Size: 434 80 32 1176 354 47 2701
Fragments arranged by size:
2701 1176 434 354 80 47 32
Bsu36I CC'TnA_GG
Cuts at: 0 2786 4824
Size: 2786 2038
BtgI C'CryG_G
Cuts at: 0 62 1817 3555 3758 4563 4824
Size: 62 1755 1738 203 805 261
Fragments arranged by size:
1755 1738 805 261 203 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2138 2931 4280 4824
Size: 2138 793 1349 544
Fragments arranged by size:
2138 1349 793 544
BtsI GCAGTG_nn'
Cuts at: 0 979 1794 2358 3663 4737 4824
Size: 979 815 564 1305 1074 87
Fragments arranged by size:
1305 1074 979 815 564 87
ClaI AT'CG_AT
Cuts at: 0 2690 3699 4824
Size: 2690 1009 1125
Fragments arranged by size:
2690 1125 1009
DraI TTT'AAA
Cuts at: 0 668 2165 4824
Size: 668 1497 2659
Fragments arranged by size:
2659 1497 668
EaeI y'GGCC_r
Cuts at: 0 1270 3378 4106 4574 4824
Size: 1270 2108 728 468 250
Fragments arranged by size:
2108 1270 728 468 250
EagI C'GGCC_G
Cuts at: 0 4574 4824
Size: 4574 250
EarI CTCTTCn'nnn_
Cuts at: 0 1124 2066 2416 2611 4277 4824
Size: 1124 942 350 195 1666 547
Fragments arranged by size:
1666 1124 942 547 350 195
EcoICRI GAG'CTC
Cuts at: 0 2409 4227 4824
Size: 2409 1818 597
Fragments arranged by size:
2409 1818 597
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 944 1105 1551 1621 1831 1912 1940 1996
Size: 944 161 446 70 210 81 28 56
Cuts at: 1996 2050 2422 2989 3494 4288 4553 4605 4736
Size: 54 372 567 505 794 265 52 131
Cuts at: 4736 4824
Size: 88
Fragments arranged by size:
944 794 567 505 446 372 265 210
161 131 88 81 70 56 54 52
28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1662 3410 4824
Size: 1662 1748 1414
Fragments arranged by size:
1748 1662 1414
EcoO109I rG'GnC_Cy
Cuts at: 0 1971 2472 3734 3874 4824
Size: 1971 501 1262 140 950
Fragments arranged by size:
1971 1262 950 501 140
EcoRI G'AATT_C
Cuts at: 0 2491 4824
Size: 2491 2333
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2427 4035 4824
Size: 2427 1608 789
Fragments arranged by size:
2427 1608 789
FspI TGC'GCA
Cuts at: 0 2858 4824
Size: 2858 1966
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 106 138 636 668 1438 1453 1470 1485
Size: 106 32 498 32 770 15 17 15
Cuts at: 1485 2914 2919 2946 2951 4824
Size: 1429 5 27 5 1873
Fragments arranged by size:
1873 1429 770 498 106 32 32 27
17 15 15 5 5
HincII GTy'rAC
Cuts at: 0 287 909 2802 4089 4824
Size: 287 622 1893 1287 735
Fragments arranged by size:
1893 1287 735 622 287
HindIII A'AGCT_T
Cuts at: 0 589 700 1477 2245 2879 2954 3201 4824
Size: 589 111 777 768 634 75 247 1623
Fragments arranged by size:
1623 777 768 634 589 247 111 75
KpnI G_GTAC'C
Cuts at: 0 248 1414 3463 3927 4824
Size: 248 1166 2049 464 897
Fragments arranged by size:
2049 1166 897 464 248
MfeI C'AATT_G
Cuts at: 0 3302 4824
Size: 3302 1522
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1056 1340 2384 3566 4824
Size: 1056 284 1044 1182 1258
Fragments arranged by size:
1258 1182 1056 1044 284
MscI TGG'CCA
Cuts at: 0 1272 3380 4108 4824
Size: 1272 2108 728 716
Fragments arranged by size:
2108 1272 728 716
MslI CAynn'nnrTG
Cuts at: 0 2821 4252 4367 4568 4609 4640 4824
Size: 2821 1431 115 201 41 31 184
Fragments arranged by size:
2821 1431 201 184 115 41 31
MspA1I CmG'CkG
Cuts at: 0 13 1950 2647 2747 2967 4824
Size: 13 1937 697 100 220 1857
Fragments arranged by size:
1937 1857 697 220 100 13
NcoI C'CATG_G
Cuts at: 0 62 1817 3555 4563 4824
Size: 62 1755 1738 1008 261
Fragments arranged by size:
1755 1738 1008 261 62
NsiI A_TGCA'T
Cuts at: 0 2982 3376 4824
Size: 2982 394 1448
Fragments arranged by size:
2982 1448 394
NspI r_CATG'y
Cuts at: 0 285 817 2820 3033 3537 4315 4824
Size: 285 532 2003 213 504 778 509
Fragments arranged by size:
2003 778 532 509 504 285 213
PasI CC'CwG_GG
Cuts at: 0 4506 4824
Size: 4506 318
PciI A'CATG_T
Cuts at: 0 3029 4311 4824
Size: 3029 1282 513
Fragments arranged by size:
3029 1282 513
PfoI T'CCnGG_A
Cuts at: 0 987 1157 2522 4824
Size: 987 170 1365 2302
Fragments arranged by size:
2302 1365 987 170
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1916 1948 4824
Size: 1916 32 2876
Fragments arranged by size:
2876 1916 32
PpuMI rG'GwC_Cy
Cuts at: 0 1971 2472 3734 3874 4824
Size: 1971 501 1262 140 950
Fragments arranged by size:
1971 1262 950 501 140
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1993 2025 4824
Size: 1993 32 2799
Fragments arranged by size:
2799 1993 32
PstI C_TGCA'G
Cuts at: 0 2021 2652 4164 4824
Size: 2021 631 1512 660
Fragments arranged by size:
2021 1512 660 631
PvuI CG_AT'CG
Cuts at: 0 1695 4824
Size: 1695 3129
PvuII CAG'CTG
Cuts at: 0 1950 2647 2747 2967 4824
Size: 1950 697 100 220 1857
Fragments arranged by size:
1950 1857 697 220 100
SacI G_AGCT'C
Cuts at: 0 2411 4229 4824
Size: 2411 1818 595
Fragments arranged by size:
2411 1818 595
SalI G'TCGA_C
Cuts at: 0 907 4087 4824
Size: 907 3180 737
Fragments arranged by size:
3180 907 737
SapI GCTCTTCn'nnn_
Cuts at: 0 1124 2416 4824
Size: 1124 1292 2408
Fragments arranged by size:
2408 1292 1124
ScaI AGT'ACT
Cuts at: 0 2002 3091 4261 4824
Size: 2002 1089 1170 563
Fragments arranged by size:
2002 1170 1089 563
SfcI C'TryA_G
Cuts at: 0 576 614 2017 2134 2282 2648 4160 4824
Size: 576 38 1403 117 148 366 1512 664
Fragments arranged by size:
1512 1403 664 576 366 148 117 38
SmlI C'TyrA_G
Cuts at: 0 1480 1935 2147 3278 3345 3750 4005 4744
Size: 1480 455 212 1131 67 405 255 739
Cuts at: 4744 4824
Size: 80
Fragments arranged by size:
1480 1131 739 455 405 255 212 80
67
SpeI A'CTAG_T
Cuts at: 0 2335 2838 4824
Size: 2335 503 1986
Fragments arranged by size:
2335 1986 503
SphI G_CATG'C
Cuts at: 0 817 2820 3537 4824
Size: 817 2003 717 1287
Fragments arranged by size:
2003 1287 817 717
SspI AAT'ATT
Cuts at: 0 4178 4824
Size: 4178 646
StuI AGG'CCT
Cuts at: 0 4629 4824
Size: 4629 195
StyI C'CwwG_G
Cuts at: 0 62 570 1405 1817 3555 4563 4630 4824
Size: 62 508 835 412 1738 1008 67 194
Fragments arranged by size:
1738 1008 835 508 412 194 67 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 133 317 499 4824
Size: 133 184 182 4325
Fragments arranged by size:
4325 184 182 133
TatI w'GTAC_w
Cuts at: 0 480 1085 2000 3089 4070 4244 4259 4824
Size: 480 605 915 1089 981 174 15 565
Fragments arranged by size:
1089 981 915 605 565 480 174 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 377 409 4824
Size: 377 32 4415
Fragments arranged by size:
4415 377 32
Tth111I GACn'n_nGTC
Cuts at: 0 120 1559 4824
Size: 120 1439 3265
Fragments arranged by size:
3265 1439 120
XbaI T'CTAG_A
Cuts at: 0 201 4824
Size: 201 4623
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1727 3018 4483 4763 4824
Size: 1727 1291 1465 280 61
Fragments arranged by size:
1727 1465 1291 280 61
XmnI GAAnn'nnTTC
Cuts at: 0 2248 3149 4177 4824
Size: 2248 901 1028 647
Fragments arranged by size:
2248 1028 901 647
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AhdI AlfI AloI
AlwNI ApoI AseI AvaI AvrII BaeI BamHI BanI
BanII BbsI BbvCI BcgI BclI BfrBI BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrDI BsrFI BsrGI BssSI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI Hin4I HincII HindIII KpnI MfeI MmeI MscI
MslI MspA1I NcoI NsiI NspI PasI PciI PfoI
PpiI PpuMI PsrI PstI PvuI PvuII SacI SalI
SapI ScaI SfcI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AleI ApaI ApaLI
AscI AsiSI BbeI BciVI BglI BglII BmgBI BmtI
BplI BsaHI BsaXI BsiWI BsmBI BspEI BsrBI BssHII
BstAPI BstZ17I CspCI DraIII DrdI EciI EcoRV FseI
FspAI HaeII HpaI KasI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI PflMI PmeI PmlI
PshAI PsiI PspOMI PspXI RsrII SacII SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI
SwaI XhoI XmaI ZraI