Restriction map for
pFN21AE2725
(Linear) (Six-base) MAPSORT of: KIEE2725.seq Check: 6405 from: 1 to: 1209
KIEE2725.orf
With 182 enzymes: SgfI *
October 2, 2018 18:08 ..
AccI GT'mk_AC
Cuts at: 0 980 1209
Size: 980 229
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 306 886 1209
Size: 306 580 323
Fragments arranged by size:
580 323 306
AfeI AGC'GCT
Cuts at: 0 669 1209
Size: 669 540
AflIII A'CryG_T
Cuts at: 0 939 1025 1209
Size: 939 86 184
Fragments arranged by size:
939 184 86
AgeI A'CCGG_T
Cuts at: 0 105 1209
Size: 105 1104
AhdI GACnn_n'nnGTC
Cuts at: 0 1095 1209
Size: 1095 114
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 214 1209
Size: 214 995
AlwNI CAG_nnn'CTG
Cuts at: 0 300 411 969 1209
Size: 300 111 558 240
Fragments arranged by size:
558 300 240 111
ApaI G_GGCC'C
Cuts at: 0 475 535 1209
Size: 475 60 674
Fragments arranged by size:
674 475 60
AscI GG'CGCG_CC
Cuts at: 0 593 1209
Size: 593 616
AvaI C'yCGr_G
Cuts at: 0 231 237 307 328 1080 1105 1209
Size: 231 6 70 21 752 25 104
Fragments arranged by size:
752 231 104 70 25 21 6
BanI G'GyrC_C
Cuts at: 0 198 222 433 439 601 730 1209
Size: 198 24 211 6 162 129 479
Fragments arranged by size:
479 211 198 162 129 24 6
BanII G_rGCy'C
Cuts at: 0 160 238 475 535 1127 1209
Size: 160 78 237 60 592 82
Fragments arranged by size:
592 237 160 82 78 60
BbeI G_GCGC'C
Cuts at: 0 226 443 605 734 1209
Size: 226 217 162 129 475
Fragments arranged by size:
475 226 217 162 129
BbsI GAAGACnn'nnnn_
Cuts at: 0 272 1209
Size: 272 937
BciVI GTATCCnnnnn_n'
Cuts at: 0 1147 1209
Size: 1147 62
BglI GCCn_nnn'nGGC
Cuts at: 0 137 421 625 1209
Size: 137 284 204 584
Fragments arranged by size:
584 284 204 137
BglII A'GATC_T
Cuts at: 0 998 1209
Size: 998 211
Bme1580I G_kGCm'C
Cuts at: 0 203 475 535 1209
Size: 203 272 60 674
Fragments arranged by size:
674 272 203 60
BmgBI CAC'GTC
Cuts at: 0 942 1209
Size: 942 267
BmrI ACTGGGnnnn_n'
Cuts at: 0 900 1209
Size: 900 309
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 967 987 1209
Size: 355 612 20 222
Fragments arranged by size:
612 355 222 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 973 1209
Size: 973 236
BsaI GGTCTCn'nnnn_
Cuts at: 0 106 1209
Size: 106 1103
BsaHI Gr'CG_yC
Cuts at: 0 223 440 602 731 1209
Size: 223 217 162 129 478
Fragments arranged by size:
478 223 217 162 129
BsaWI w'CCGG_w
Cuts at: 0 105 1209
Size: 105 1104
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 766 796 1209
Size: 766 30 413
Fragments arranged by size:
766 413 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 478 865 1209
Size: 478 387 344
Fragments arranged by size:
478 387 344
BseYI C'CCAG_C
Cuts at: 0 211 226 315 637 670 1175 1209
Size: 211 15 89 322 33 505 34
Fragments arranged by size:
505 322 211 89 34 33 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 721 748 949 1209
Size: 210 511 27 201 260
Fragments arranged by size:
511 260 210 201 27
BsiEI CG_ry'CG
Cuts at: 0 120 141 341 425 512 590 620 629
Size: 120 21 200 84 87 78 30 9
Cuts at: 629 777 1209
Size: 148 432
Fragments arranged by size:
432 200 148 120 87 84 78 30
21 9
BsiHKAI G_wGCw'C
Cuts at: 0 184 1209
Size: 184 1025
BsmBI CGTCTCn'nnnn_
Cuts at: 0 148 1209
Size: 148 1061
Bsp1286I G_dGCh'C
Cuts at: 0 160 184 203 238 475 535 1127 1209
Size: 160 24 19 35 237 60 592 82
Fragments arranged by size:
592 237 160 82 60 35 24 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 41 1209
Size: 41 1168
BsrBI CCG'CTC
Cuts at: 0 524 1209
Size: 524 685
BsrDI GCAATG_nn'
Cuts at: 0 23 1209
Size: 23 1186
BsrFI r'CCGG_y
Cuts at: 0 105 283 430 436 565 589 628 754
Size: 105 178 147 6 129 24 39 126
Cuts at: 754 824 1209
Size: 70 385
Fragments arranged by size:
385 178 147 129 126 105 70 39
24 6
BssHII G'CGCG_C
Cuts at: 0 394 593 1209
Size: 394 199 616
Fragments arranged by size:
616 394 199
BstAPI GCAn_nnn'nTGC
Cuts at: 0 188 1209
Size: 188 1021
BstEII G'GTnAC_C
Cuts at: 0 798 1209
Size: 798 411
BstXI CCAn_nnnn'nTGG
Cuts at: 0 48 1209
Size: 48 1161
BstYI r'GATC_y
Cuts at: 0 998 1209
Size: 998 211
BtgI C'CryG_G
Cuts at: 0 53 416 485 506 605 894 1209
Size: 53 363 69 21 99 289 315
Fragments arranged by size:
363 315 289 99 69 53 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 132 1209
Size: 132 1077
DraIII CAC_nnn'GTG
Cuts at: 0 188 1209
Size: 188 1021
EaeI y'GGCC_r
Cuts at: 0 50 138 413 422 509 567 587 617
Size: 50 88 275 9 87 58 20 30
Cuts at: 617 626 662 1209
Size: 9 36 547
Fragments arranged by size:
547 275 88 87 58 50 36 30
20 9 9
EagI C'GGCC_G
Cuts at: 0 138 422 509 587 617 626 1209
Size: 138 284 87 78 30 9 583
Fragments arranged by size:
583 284 138 87 78 30 9
EarI CTCTTCn'nnn_
Cuts at: 0 868 1209
Size: 868 341
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 483 713 894 1209
Size: 483 230 181 315
Fragments arranged by size:
483 315 230 181
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 306 355 886 967 987 1209
Size: 306 49 531 81 20 222
Fragments arranged by size:
531 306 222 81 49 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 991 1209
Size: 991 218
EcoO109I rG'GnC_Cy
Cuts at: 0 472 1209
Size: 472 737
HaeII r_GCGC'y
Cuts at: 0 226 316 349 443 605 638 671 734
Size: 226 90 33 94 162 33 33 63
Cuts at: 734 1209
Size: 475
Fragments arranged by size:
475 226 162 94 90 63 33 33
33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 766 798 1081 1113 1209
Size: 766 32 283 32 96
Fragments arranged by size:
766 283 96 32 32
HincII GTy'rAC
Cuts at: 0 981 1209
Size: 981 228
KasI G'GCGC_C
Cuts at: 0 222 439 601 730 1209
Size: 222 217 162 129 479
Fragments arranged by size:
479 222 217 162 129
MluI A'CGCG_T
Cuts at: 0 1025 1209
Size: 1025 184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 809 932 1118 1209
Size: 53 756 123 186 91
Fragments arranged by size:
756 186 123 91 53
MscI TGG'CCA
Cuts at: 0 52 1209
Size: 52 1157
MslI CAynn'nnrTG
Cuts at: 0 175 808 1209
Size: 175 633 401
Fragments arranged by size:
633 401 175
MspA1I CmG'CkG
Cuts at: 0 300 411 418 458 508 607 795 831
Size: 300 111 7 40 50 99 188 36
Cuts at: 831 896 966 1061 1209
Size: 65 70 95 148
Fragments arranged by size:
300 188 148 111 99 95 70 65
50 40 36 7
NaeI GCC'GGC
Cuts at: 0 438 567 591 756 1209
Size: 438 129 24 165 453
Fragments arranged by size:
453 438 165 129 24
NarI GG'CG_CC
Cuts at: 0 223 440 602 731 1209
Size: 223 217 162 129 478
Fragments arranged by size:
478 223 217 162 129
NcoI C'CATG_G
Cuts at: 0 53 1209
Size: 53 1156
NgoMIV G'CCGG_C
Cuts at: 0 436 565 589 754 1209
Size: 436 129 24 165 455
Fragments arranged by size:
455 436 165 129 24
NotI GC'GGCC_GC
Cuts at: 0 509 617 1209
Size: 509 108 592
Fragments arranged by size:
592 509 108
PflMI CCAn_nnn'nTGG
Cuts at: 0 1166 1209
Size: 1166 43
PfoI T'CCnGG_A
Cuts at: 0 944 1209
Size: 944 265
PspOMI G'GGCC_C
Cuts at: 0 471 531 1209
Size: 471 60 678
Fragments arranged by size:
678 471 60
PspXI vC'TCGA_Gb
Cuts at: 0 1080 1209
Size: 1080 129
PstI C_TGCA'G
Cuts at: 0 974 1209
Size: 974 235
PvuII CAG'CTG
Cuts at: 0 300 411 795 831 966 1209
Size: 300 111 384 36 135 243
Fragments arranged by size:
384 300 243 135 111 36
SacII CC_GC'GG
Cuts at: 0 419 509 608 897 1209
Size: 419 90 99 289 312
Fragments arranged by size:
419 312 289 99 90
SalI G'TCGA_C
Cuts at: 0 979 1209
Size: 979 230
SfcI C'TryA_G
Cuts at: 0 970 1209
Size: 970 239
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 421 625 1209
Size: 421 204 584
Fragments arranged by size:
584 421 204
SfoI GGC'GCC
Cuts at: 0 224 441 603 732 1209
Size: 224 217 162 129 477
Fragments arranged by size:
477 224 217 162 129
SgrAI Cr'CCGG_yG
Cuts at: 0 754 1209
Size: 754 455
SmaI CCC'GGG
Cuts at: 0 233 1209
Size: 233 976
SmlI C'TyrA_G
Cuts at: 0 988 1080 1209
Size: 988 92 129
Fragments arranged by size:
988 129 92
SrfI GCCC'GGGC
Cuts at: 0 233 1209
Size: 233 976
StyI C'CwwG_G
Cuts at: 0 53 446 1209
Size: 53 393 763
Fragments arranged by size:
763 393 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 130 1209
Size: 130 1079
TatI w'GTAC_w
Cuts at: 0 1185 1209
Size: 1185 24
Tth111I GACn'n_nGTC
Cuts at: 0 337 804 1209
Size: 337 467 405
Fragments arranged by size:
467 405 337
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 45 1134 1209
Size: 45 1089 75
Fragments arranged by size:
1089 75 45
XhoI C'TCGA_G
Cuts at: 0 1080 1209
Size: 1080 129
XmaI C'CCGG_G
Cuts at: 0 231 1209
Size: 231 978
Enzymes that do cut:
AccI AcuI AfeI AflIII AgeI AhdI AlfI AlwNI
ApaI AscI AvaI BanI BanII BbeI BbsI BciVI
BglI BglII Bme1580I BmgBI BmrI BpmI BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BstAPI
BstEII BstXI BstYI BtgI BtgZI DraIII EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII
KasI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI PflMI PfoI PspOMI PspXI PstI
PvuII SacII SalI SfcI SfiI SfoI SgrAI SmaI
SmlI SrfI StyI TaqII TatI Tth111I XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AleI AloI ApaLI
ApoI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI
BclI BfrBI BlpI BmtI BplI Bpu10I BsaAI BsaBI
BsiWI BsmI BspEI BspHI BsrGI BssSI BstBI BstZ17I
Bsu36I BtsI ClaI CspCI DraI DrdI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI
MfeI NdeI NheI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI
PvuI RsrII SacI SanDI SapI SbfI ScaI SexAI
SgfI SnaBI SpeI SphI SspI StuI SwaI TstI
XbaI XmnI ZraI