Restriction map for
pFN21AE2723
(Linear) (Six-base) MAPSORT of: KIEE2723.seq Check: 4750 from: 1 to: 906
KIEE2723.orf
With 182 enzymes: SgfI *
July 7, 2018 15:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 886 906
Size: 886 20
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 742 906
Size: 247 495 164
Fragments arranged by size:
495 247 164
AleI CACnn'nnGTG
Cuts at: 0 646 906
Size: 646 260
ApoI r'AATT_y
Cuts at: 0 859 906
Size: 859 47
AvaI C'yCGr_G
Cuts at: 0 844 906
Size: 844 62
BanI G'GyrC_C
Cuts at: 0 184 550 906
Size: 184 366 356
Fragments arranged by size:
366 356 184
BbeI G_GCGC'C
Cuts at: 0 188 906
Size: 188 718
BfrBI ATG'CAT
Cuts at: 0 801 906
Size: 801 105
Bme1580I G_kGCm'C
Cuts at: 0 262 906
Size: 262 644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 906
Size: 508 398
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 906
Size: 406 500
BsaHI Gr'CG_yC
Cuts at: 0 153 185 906
Size: 153 32 721
Fragments arranged by size:
721 153 32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 292 322 906
Size: 292 30 584
Fragments arranged by size:
584 292 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 256 316 409 769 772 775 862 906
Size: 256 60 93 360 3 3 87 44
Fragments arranged by size:
360 256 93 87 60 44 3 3
BseYI C'CCAG_C
Cuts at: 0 15 906
Size: 15 891
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 549 781 824 906
Size: 549 232 43 82
Fragments arranged by size:
549 232 82 43
BsmBI CGTCTCn'nnnn_
Cuts at: 0 226 724 906
Size: 226 498 182
Fragments arranged by size:
498 226 182
Bsp1286I G_dGCh'C
Cuts at: 0 262 906
Size: 262 644
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 886 906
Size: 886 20
BsrFI r'CCGG_y
Cuts at: 0 130 906
Size: 130 776
BssHII G'CGCG_C
Cuts at: 0 20 906
Size: 20 886
BstAPI GCAn_nnn'nTGC
Cuts at: 0 266 538 906
Size: 266 272 368
Fragments arranged by size:
368 272 266
BstYI r'GATC_y
Cuts at: 0 245 851 906
Size: 245 606 55
Fragments arranged by size:
606 245 55
BtgI C'CryG_G
Cuts at: 0 374 381 790 906
Size: 374 7 409 116
Fragments arranged by size:
409 374 116 7
DraIII CAC_nnn'GTG
Cuts at: 0 266 510 906
Size: 266 244 396
Fragments arranged by size:
396 266 244
EaeI y'GGCC_r
Cuts at: 0 317 501 906
Size: 317 184 405
Fragments arranged by size:
405 317 184
EarI CTCTTCn'nnn_
Cuts at: 0 382 906
Size: 382 524
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 508 742 906
Size: 247 261 234 164
Fragments arranged by size:
261 247 234 164
EcoO109I rG'GnC_Cy
Cuts at: 0 102 774 906
Size: 102 672 132
Fragments arranged by size:
672 132 102
HaeII r_GCGC'y
Cuts at: 0 188 641 906
Size: 188 453 265
Fragments arranged by size:
453 265 188
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 21 36 53 68 906
Size: 21 15 17 15 838
Fragments arranged by size:
838 21 17 15 15
KasI G'GCGC_C
Cuts at: 0 184 906
Size: 184 722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 906
Size: 54 852
MscI TGG'CCA
Cuts at: 0 503 906
Size: 503 403
MslI CAynn'nnrTG
Cuts at: 0 358 646 673 880 906
Size: 358 288 27 207 26
Fragments arranged by size:
358 288 207 27 26
NarI GG'CG_CC
Cuts at: 0 185 906
Size: 185 721
NcoI C'CATG_G
Cuts at: 0 381 790 906
Size: 381 409 116
Fragments arranged by size:
409 381 116
NsiI A_TGCA'T
Cuts at: 0 803 906
Size: 803 103
NspI r_CATG'y
Cuts at: 0 805 906
Size: 805 101
PasI CC'CwG_GG
Cuts at: 0 444 609 906
Size: 444 165 297
Fragments arranged by size:
444 297 165
PfoI T'CCnGG_A
Cuts at: 0 329 627 906
Size: 329 298 279
Fragments arranged by size:
329 298 279
PpuMI rG'GwC_Cy
Cuts at: 0 102 906
Size: 102 804
PspXI vC'TCGA_Gb
Cuts at: 0 844 906
Size: 844 62
PstI C_TGCA'G
Cuts at: 0 497 906
Size: 497 409
SapI GCTCTTCn'nnn_
Cuts at: 0 382 906
Size: 382 524
SfcI C'TryA_G
Cuts at: 0 40 493 906
Size: 40 453 413
Fragments arranged by size:
453 413 40
SfoI GGC'GCC
Cuts at: 0 186 906
Size: 186 720
SgrAI Cr'CCGG_yG
Cuts at: 0 130 906
Size: 130 776
SmlI C'TyrA_G
Cuts at: 0 421 844 906
Size: 421 423 62
Fragments arranged by size:
423 421 62
SphI G_CATG'C
Cuts at: 0 805 906
Size: 805 101
StuI AGG'CCT
Cuts at: 0 469 906
Size: 469 437
StyI C'CwwG_G
Cuts at: 0 320 381 464 790 906
Size: 320 61 83 326 116
Fragments arranged by size:
326 320 116 83 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 331 906
Size: 331 575
XhoI C'TCGA_G
Cuts at: 0 844 906
Size: 844 62
XmnI GAAnn'nnTTC
Cuts at: 0 889 906
Size: 889 17
Enzymes that do cut:
AarI AcuI AleI ApoI AvaI BanI BbeI BfrBI
Bme1580I BpmI BpuEI BsaHI BsaXI BseRI BseYI BsgI
BsmBI Bsp1286I BspMI BsrFI BssHII BstAPI BstYI BtgI
DraIII EaeI EarI Eco57MI EcoO109I HaeII Hin4I KasI
MmeI MscI MslI NarI NcoI NsiI NspI PasI
PfoI PpuMI PspXI PstI SapI SfcI SfoI SgrAI
SmlI SphI StuI StyI TaqII XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI BmgBI BmrI BmtI
BplI Bpu10I BsaI BsaAI BsaBI BsaWI BsiEI BsiHKAI
BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MspA1I NaeI NdeI NgoMIV NheI
NotI NruI PacI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI
SmaI SnaBI SpeI SrfI SspI SwaI TatI TstI
Tth111I XbaI XcmI XmaI ZraI