Restriction map for
pFN21AE2722
(Linear) (Six-base) MAPSORT of: KIEE2722.seq Check: 2665 from: 1 to: 900
KIEE2722.orf
With 182 enzymes: SgfI *
June 6, 2018 17:50 ..
AatII G_ACGT'C
Cuts at: 0 165 900
Size: 165 735
AccI GT'mk_AC
Cuts at: 0 341 551 900
Size: 341 210 349
Fragments arranged by size:
349 341 210
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 648 900
Size: 648 252
AfeI AGC'GCT
Cuts at: 0 572 900
Size: 572 328
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 424 456 900
Size: 424 32 444
Fragments arranged by size:
444 424 32
AlwNI CAG_nnn'CTG
Cuts at: 0 468 900
Size: 468 432
ApaI G_GGCC'C
Cuts at: 0 183 900
Size: 183 717
AvaI C'yCGr_G
Cuts at: 0 17 170 900
Size: 17 153 730
Fragments arranged by size:
730 153 17
BanII G_rGCy'C
Cuts at: 0 183 900
Size: 183 717
BbvCI CC'TCA_GC
Cuts at: 0 290 900
Size: 290 610
BglII A'GATC_T
Cuts at: 0 407 900
Size: 407 493
BlpI GC'TnA_GC
Cuts at: 0 457 900
Size: 457 443
Bme1580I G_kGCm'C
Cuts at: 0 183 900
Size: 183 717
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 775 900
Size: 343 432 125
Fragments arranged by size:
432 343 125
Bpu10I CC'TnA_GC
Cuts at: 0 290 900
Size: 290 610
BsaI GGTCTCn'nnnn_
Cuts at: 0 513 586 900
Size: 513 73 314
Fragments arranged by size:
513 314 73
BsaAI yAC'GTr
Cuts at: 0 186 279 900
Size: 186 93 621
Fragments arranged by size:
621 186 93
BsaHI Gr'CG_yC
Cuts at: 0 95 162 338 900
Size: 95 67 176 562
Fragments arranged by size:
562 176 95 67
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 870 900
Size: 870 30
BseYI C'CCAG_C
Cuts at: 0 444 543 576 900
Size: 444 99 33 324
Fragments arranged by size:
444 324 99 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 375 784 900
Size: 375 409 116
Fragments arranged by size:
409 375 116
BsiHKAI G_wGCw'C
Cuts at: 0 329 388 789 900
Size: 329 59 401 111
Fragments arranged by size:
401 329 111 59
Bsp1286I G_dGCh'C
Cuts at: 0 183 329 388 789 900
Size: 183 146 59 401 111
Fragments arranged by size:
401 183 146 111 59
BsrDI GCAATG_nn'
Cuts at: 0 515 820 900
Size: 515 305 80
Fragments arranged by size:
515 305 80
BstAPI GCAn_nnn'nTGC
Cuts at: 0 272 900
Size: 272 628
BstXI CCAn_nnnn'nTGG
Cuts at: 0 321 900
Size: 321 579
BstYI r'GATC_y
Cuts at: 0 407 419 900
Size: 407 12 481
Fragments arranged by size:
481 407 12
BtgI C'CryG_G
Cuts at: 0 116 227 900
Size: 116 111 673
Fragments arranged by size:
673 116 111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 138 900
Size: 138 762
EaeI y'GGCC_r
Cuts at: 0 230 311 900
Size: 230 81 589
Fragments arranged by size:
589 230 81
EarI CTCTTCn'nnn_
Cuts at: 0 516 900
Size: 516 384
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 648 775 900
Size: 343 305 127 125
Fragments arranged by size:
343 305 127 125
EcoNI CCTnn'n_nnAGG
Cuts at: 0 48 377 900
Size: 48 329 523
Fragments arranged by size:
523 329 48
EcoO109I rG'GnC_Cy
Cuts at: 0 179 318 900
Size: 179 139 582
Fragments arranged by size:
582 179 139
HaeII r_GCGC'y
Cuts at: 0 544 574 900
Size: 544 30 326
Fragments arranged by size:
544 326 30
HincII GTy'rAC
Cuts at: 0 342 369 809 900
Size: 342 27 440 91
Fragments arranged by size:
440 342 91 27
MscI TGG'CCA
Cuts at: 0 232 313 900
Size: 232 81 587
Fragments arranged by size:
587 232 81
MslI CAynn'nnrTG
Cuts at: 0 44 694 825 900
Size: 44 650 131 75
Fragments arranged by size:
650 131 75 44
MspA1I CmG'CkG
Cuts at: 0 306 734 900
Size: 306 428 166
Fragments arranged by size:
428 306 166
NcoI C'CATG_G
Cuts at: 0 227 900
Size: 227 673
PfoI T'CCnGG_A
Cuts at: 0 642 900
Size: 642 258
PmlI CAC'GTG
Cuts at: 0 186 279 900
Size: 186 93 621
Fragments arranged by size:
621 186 93
PsiI TTA'TAA
Cuts at: 0 527 900
Size: 527 373
PspOMI G'GGCC_C
Cuts at: 0 179 900
Size: 179 721
PstI C_TGCA'G
Cuts at: 0 242 739 900
Size: 242 497 161
Fragments arranged by size:
497 242 161
PvuII CAG'CTG
Cuts at: 0 306 734 900
Size: 306 428 166
Fragments arranged by size:
428 306 166
SalI G'TCGA_C
Cuts at: 0 340 900
Size: 340 560
SfcI C'TryA_G
Cuts at: 0 238 735 900
Size: 238 497 165
Fragments arranged by size:
497 238 165
SmaI CCC'GGG
Cuts at: 0 19 172 900
Size: 19 153 728
Fragments arranged by size:
728 153 19
StyI C'CwwG_G
Cuts at: 0 227 314 614 900
Size: 227 87 300 286
Fragments arranged by size:
300 286 227 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 535 900
Size: 535 365
XmaI C'CCGG_G
Cuts at: 0 17 170 900
Size: 17 153 730
Fragments arranged by size:
730 153 17
XmnI GAAnn'nnTTC
Cuts at: 0 714 900
Size: 714 186
ZraI GAC'GTC
Cuts at: 0 163 900
Size: 163 737
Enzymes that do cut:
AatII AccI AcuI AfeI AloI AlwNI ApaI AvaI
BanII BbvCI BglII BlpI Bme1580I BpmI Bpu10I BsaI
BsaAI BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrDI
BstAPI BstXI BstYI BtgI BtgZI EaeI EarI Eco57MI
EcoNI EcoO109I HaeII HincII MscI MslI MspA1I NcoI
PfoI PmlI PsiI PspOMI PstI PvuII SalI SfcI
SmaI StyI TaqII XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbsI BcgI BciVI BclI BfrBI
BglI BmgBI BmrI BmtI BplI BpuEI BsaBI BsaWI
BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PspXI PsrI PvuI RsrII SacI SacII SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI Tth111I XbaI XcmI XhoI