Restriction map for
pFN21AE2720
(Linear) (Six-base) MAPSORT of: KIEE2720.seq Check: 4915 from: 1 to: 924
KIEE2720.orf
With 182 enzymes: SgfI *
June 6, 2018 17:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 517 924
Size: 517 407
AccI GT'mk_AC
Cuts at: 0 683 924
Size: 683 241
Acc65I G'GTAC_C
Cuts at: 0 835 924
Size: 835 89
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 924
Size: 577 347
AflIII A'CryG_T
Cuts at: 0 249 924
Size: 249 675
ApoI r'AATT_y
Cuts at: 0 451 849 908 924
Size: 451 398 59 16
Fragments arranged by size:
451 398 59 16
BanI G'GyrC_C
Cuts at: 0 706 835 924
Size: 706 129 89
Fragments arranged by size:
706 129 89
BanII G_rGCy'C
Cuts at: 0 377 924
Size: 377 547
BbsI GAAGACnn'nnnn_
Cuts at: 0 179 924
Size: 179 745
BciVI GTATCCnnnnn_n'
Cuts at: 0 197 924
Size: 197 727
BclI T'GATC_A
Cuts at: 0 108 222 924
Size: 108 114 702
Fragments arranged by size:
702 114 108
BglII A'GATC_T
Cuts at: 0 555 805 924
Size: 555 250 119
Fragments arranged by size:
555 250 119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 198 924
Size: 198 726
BsaWI w'CCGG_w
Cuts at: 0 200 924
Size: 200 724
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 360 363 924
Size: 360 3 561
Fragments arranged by size:
561 360 3
BseYI C'CCAG_C
Cuts at: 0 710 924
Size: 710 214
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 873 924
Size: 873 51
BsiHKAI G_wGCw'C
Cuts at: 0 377 924
Size: 377 547
Bsp1286I G_dGCh'C
Cuts at: 0 377 924
Size: 377 547
BspEI T'CCGG_A
Cuts at: 0 200 924
Size: 200 724
BspHI T'CATG_A
Cuts at: 0 333 692 924
Size: 333 359 232
Fragments arranged by size:
359 333 232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 517 924
Size: 517 407
BstXI CCAn_nnnn'nTGG
Cuts at: 0 138 627 924
Size: 138 489 297
Fragments arranged by size:
489 297 138
BstYI r'GATC_y
Cuts at: 0 495 555 805 924
Size: 495 60 250 119
Fragments arranged by size:
495 250 119 60
Bsu36I CC'TnA_GG
Cuts at: 0 343 924
Size: 343 581
BtgI C'CryG_G
Cuts at: 0 7 924
Size: 7 917
ClaI AT'CG_AT
Cuts at: 0 896 924
Size: 896 28
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 633 668 924
Size: 633 35 256
Fragments arranged by size:
633 256 35
DraIII CAC_nnn'GTG
Cuts at: 0 918 924
Size: 918 6
EaeI y'GGCC_r
Cuts at: 0 4 188 924
Size: 4 184 736
Fragments arranged by size:
736 184 4
EarI CTCTTCn'nnn_
Cuts at: 0 237 254 924
Size: 237 17 670
Fragments arranged by size:
670 237 17
EcoICRI GAG'CTC
Cuts at: 0 375 924
Size: 375 549
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 924
Size: 577 347
EcoO109I rG'GnC_Cy
Cuts at: 0 56 214 924
Size: 56 158 710
Fragments arranged by size:
710 158 56
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 283 924
Size: 283 641
HaeII r_GCGC'y
Cuts at: 0 747 924
Size: 747 177
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 797 829 924
Size: 797 32 95
Fragments arranged by size:
797 95 32
HindIII A'AGCT_T
Cuts at: 0 145 924
Size: 145 779
KpnI G_GTAC'C
Cuts at: 0 839 924
Size: 839 85
MluI A'CGCG_T
Cuts at: 0 249 924
Size: 249 675
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 924
Size: 7 917
MslI CAynn'nnrTG
Cuts at: 0 446 924
Size: 446 478
MspA1I CmG'CkG
Cuts at: 0 576 882 924
Size: 576 306 42
Fragments arranged by size:
576 306 42
NcoI C'CATG_G
Cuts at: 0 7 924
Size: 7 917
NdeI CA'TA_TG
Cuts at: 0 702 924
Size: 702 222
NspI r_CATG'y
Cuts at: 0 445 797 924
Size: 445 352 127
Fragments arranged by size:
445 352 127
PflMI CCAn_nnn'nTGG
Cuts at: 0 13 678 924
Size: 13 665 246
Fragments arranged by size:
665 246 13
PpuMI rG'GwC_Cy
Cuts at: 0 56 924
Size: 56 868
PshAI GACnn'nnGTC
Cuts at: 0 828 924
Size: 828 96
PstI C_TGCA'G
Cuts at: 0 613 924
Size: 613 311
PvuII CAG'CTG
Cuts at: 0 882 924
Size: 882 42
SacI G_AGCT'C
Cuts at: 0 377 924
Size: 377 547
SapI GCTCTTCn'nnn_
Cuts at: 0 254 924
Size: 254 670
SfcI C'TryA_G
Cuts at: 0 236 388 609 924
Size: 236 152 221 315
Fragments arranged by size:
315 236 221 152
SmlI C'TyrA_G
Cuts at: 0 177 924
Size: 177 747
StuI AGG'CCT
Cuts at: 0 780 924
Size: 780 144
StyI C'CwwG_G
Cuts at: 0 7 602 924
Size: 7 595 322
Fragments arranged by size:
595 322 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 724 924
Size: 724 200
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 318 924
Size: 318 606
XmnI GAAnn'nnTTC
Cuts at: 0 569 924
Size: 569 355
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII ApoI BanI BanII
BbsI BciVI BclI BglII BpuEI BsaWI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI BstXI BstYI
Bsu36I BtgI ClaI CspCI DraIII EaeI EarI EcoICRI
Eco57MI EcoO109I FalI HaeII Hin4I HindIII KpnI MluI
MmeI MslI MspA1I NcoI NdeI NspI PflMI PpuMI
PshAI PstI PvuII SacI SapI SfcI SmlI StuI
StyI TaqII XcmI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AleI AlfI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BfrBI BglI
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BsaI BsaAI BsaBI BsaHI BsaXI BsiEI BsiWI BsmI
BsmBI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I BtgZI BtsI DraI DrdI EagI
EciI EcoNI EcoRI EcoRV FseI FspI FspAI HincII
HpaI KasI MfeI MscI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TatI TstI Tth111I XbaI XhoI XmaI ZraI