(Linear) (Six-base) MAPSORT of: KIEE2716.seq  Check: 4268  from: 1  to: 705



KIEE2716.orf

 With 182 enzymes: SgfI * 

                        January 30, 2018 10:51  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     675     705 
   Size:        675      30
 
AhdI GACnn_n'nnGTC

Cuts at:      0     621     705 
   Size:        621      84
 
AlwNI CAG_nnn'CTG

Cuts at:      0     389     705 
   Size:        389     316
 
ApoI r'AATT_y

Cuts at:      0     459     705 
   Size:        459     246
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     332     628     705 
   Size:        332     296      77

  Fragments arranged by size: 

                 332     296      77
 
BbvCI CC'TCA_GC

Cuts at:      0     409     705 
   Size:        409     296
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     231     705 
   Size:        231     474
 
BglI GCCn_nnn'nGGC

Cuts at:      0     386     705 
   Size:        386     319
 
BglII A'GATC_T

Cuts at:      0     331     705 
   Size:        331     374
 
Bpu10I CC'TnA_GC

Cuts at:      0     409     705 
   Size:        409     296
 
BsiHKAI G_wGCw'C

Cuts at:      0     303     705 
   Size:        303     402
 
BsmI GAATG_Cn'

Cuts at:      0     691     705 
   Size:        691      14
 
Bsp1286I G_dGCh'C

Cuts at:      0     303     705 
   Size:        303     402
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     675     705 
   Size:        675      30
 
BsrDI GCAATG_nn'

Cuts at:      0     520     705 
   Size:        520     185
 
BstYI r'GATC_y

Cuts at:      0     331     705 
   Size:        331     374
 
BtgI C'CryG_G

Cuts at:      0     175     224     351     705 
   Size:        175      49     127     354

  Fragments arranged by size: 

                 354     175     127      49
 
EaeI y'GGCC_r

Cuts at:      0     178     416     705 
   Size:        178     238     289

  Fragments arranged by size: 

                 289     238     178
 
EarI CTCTTCn'nnn_

Cuts at:      0     285     705 
   Size:        285     420
 
EcoO109I rG'GnC_Cy

Cuts at:      0     198     705 
   Size:        198     507
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      53      85     607     639     705 
   Size:         53      32     522      32      66

  Fragments arranged by size: 

                 522      66      53      32      32
 
HindIII A'AGCT_T

Cuts at:      0     440     705 
   Size:        440     265
 
MscI TGG'CCA

Cuts at:      0     180     418     705 
   Size:        180     238     287

  Fragments arranged by size: 

                 287     238     180
 
MspA1I CmG'CkG

Cuts at:      0     549     705 
   Size:        549     156
 
NcoI C'CATG_G

Cuts at:      0     175     224     705 
   Size:        175      49     481

  Fragments arranged by size: 

                 481     175      49
 
PpuMI rG'GwC_Cy

Cuts at:      0     198     705 
   Size:        198     507
 
PvuII CAG'CTG

Cuts at:      0     549     705 
   Size:        549     156
 
SapI GCTCTTCn'nnn_

Cuts at:      0     285     705 
   Size:        285     420
 
StyI C'CwwG_G

Cuts at:      0     175     224     705 
   Size:        175      49     481

  Fragments arranged by size: 

                 481     175      49
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     364     705 
   Size:        364     341
 
TatI w'GTAC_w

Cuts at:      0      51     705 
   Size:         51     654
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     188     705 
   Size:        188     517
 

 Enzymes that do cut:

     AarI     AhdI    AlwNI     ApoI     BbsI    BbvCI    BciVI     BglI
    BglII   Bpu10I  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI    BstYI
     BtgI     EaeI     EarI EcoO109I    Hin4I  HindIII     MscI   MspA1I
     NcoI    PpuMI    PvuII     SapI     StyI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BcgI     BclI    BfrBI     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI  Eco57MI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI