(Linear) (Six-base) MAPSORT of: KIEE2713.seq  Check: 2330  from: 1  to: 957



KIEE2713.orf

 With 182 enzymes: SgfI * 

                        January 30, 2018 10:51  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     957 
   Size:        119     838
 
AflIII A'CryG_T

Cuts at:      0     326     527     957 
   Size:        326     201     430

  Fragments arranged by size: 

                 430     326     201
 
ApoI r'AATT_y

Cuts at:      0     445     957 
   Size:        445     512
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     267     300     957 
   Size:        267      33     657

  Fragments arranged by size: 

                 657     267      33
 
BamHI G'GATC_C

Cuts at:      0     141     957 
   Size:        141     816
 
BanI G'GyrC_C

Cuts at:      0     768     957 
   Size:        768     189
 
BanII G_rGCy'C

Cuts at:      0     186     957 
   Size:        186     771
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     615     957 
   Size:        615     342
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     146     176     718     748     957 
   Size:        146      30     542      30     209

  Fragments arranged by size: 

                 542     209     146      30      30
 
BseYI C'CCAG_C

Cuts at:      0      68     642     922     957 
   Size:         68     574     280      35

  Fragments arranged by size: 

                 574     280      68      35
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      15     388     957 
   Size:         15     373     569

  Fragments arranged by size: 

                 569     373      15
 
Bsp1286I G_dGCh'C

Cuts at:      0     186     957 
   Size:        186     771
 
BspHI T'CATG_A

Cuts at:      0     591     957 
   Size:        591     366
 
BsrDI GCAATG_nn'

Cuts at:      0     764     957 
   Size:        764     193
 
BstEII G'GTnAC_C

Cuts at:      0     267     957 
   Size:        267     690
 
BstYI r'GATC_y

Cuts at:      0     141     557     944     957 
   Size:        141     416     387      13

  Fragments arranged by size: 

                 416     387     141      13
 
BtgI C'CryG_G

Cuts at:      0     454     957 
   Size:        454     503
 
BtsI GCAGTG_nn'

Cuts at:      0     364     957 
   Size:        364     593
 
DraI TTT'AAA

Cuts at:      0     258     957 
   Size:        258     699
 
DraIII CAC_nnn'GTG

Cuts at:      0     935     957 
   Size:        935      22
 
EarI CTCTTCn'nnn_

Cuts at:      0     424     957 
   Size:        424     533
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     957 
   Size:        119     838
 
EcoO109I rG'GnC_Cy

Cuts at:      0      33     513     957 
   Size:         33     480     444

  Fragments arranged by size: 

                 480     444      33
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     549     581     957 
   Size:        549      32     376

  Fragments arranged by size: 

                 549     376      32
 
MspA1I CmG'CkG

Cuts at:      0      72     631     957 
   Size:         72     559     326

  Fragments arranged by size: 

                 559     326      72
 
PpuMI rG'GwC_Cy

Cuts at:      0      33     957 
   Size:         33     924
 
PvuII CAG'CTG

Cuts at:      0     631     957 
   Size:        631     326
 
SanDI GG'GwC_CC

Cuts at:      0      33     957 
   Size:         33     924
 
SexAI A'CCwGG_T

Cuts at:      0     931     957 
   Size:        931      26
 
StyI C'CwwG_G

Cuts at:      0     263     957 
   Size:        263     694
 
SwaI ATTT'AAAT

Cuts at:      0     258     957 
   Size:        258     699
 
TatI w'GTAC_w

Cuts at:      0     524     878     957 
   Size:        524     354      79

  Fragments arranged by size: 

                 524     354      79
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     146     178     957 
   Size:        146      32     779

  Fragments arranged by size: 

                 779     146      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     756     957 
   Size:        756     201
 

 Enzymes that do cut:

     AcuI   AflIII     ApoI     BaeI    BamHI     BanI    BanII     BmrI
    BsaXI    BseYI    BsmBI Bsp1286I    BspHI    BsrDI   BstEII    BstYI
     BtgI     BtsI     DraI   DraIII     EarI  Eco57MI EcoO109I    Hin4I
   MspA1I    PpuMI    PvuII    SanDI    SexAI     StyI     SwaI     TatI
     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI     SapI     SbfI     ScaI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI    TaqII  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI