Restriction map for
pFN21AE2711
(Linear) (Six-base) MAPSORT of: KIEE2711.seq Check: 675 from: 1 to: 2793
KIEE2711.orf
With 182 enzymes: SgfI *
January 19, 2018 17:31 ..
AccI GT'mk_AC
Cuts at: 0 1355 2793
Size: 1355 1438
Acc65I G'GTAC_C
Cuts at: 0 2536 2793
Size: 2536 257
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 782 911 1217 2743 2793
Size: 637 145 129 306 1526 50
Fragments arranged by size:
1526 637 306 145 129 50
AgeI A'CCGG_T
Cuts at: 0 1661 2793
Size: 1661 1132
AleI CACnn'nnGTG
Cuts at: 0 2122 2793
Size: 2122 671
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1034 1066 2793
Size: 1034 32 1727
Fragments arranged by size:
1727 1034 32
ApoI r'AATT_y
Cuts at: 0 63 721 1006 1342 2793
Size: 63 658 285 336 1451
Fragments arranged by size:
1451 658 336 285 63
AvaI C'yCGr_G
Cuts at: 0 95 2194 2576 2793
Size: 95 2099 382 217
Fragments arranged by size:
2099 382 217 95
BanI G'GyrC_C
Cuts at: 0 2020 2536 2793
Size: 2020 516 257
Fragments arranged by size:
2020 516 257
BanII G_rGCy'C
Cuts at: 0 958 1077 1271 1382 2793
Size: 958 119 194 111 1411
Fragments arranged by size:
1411 958 194 119 111
BbeI G_GCGC'C
Cuts at: 0 2024 2793
Size: 2024 769
BbsI GAAGACnn'nnnn_
Cuts at: 0 904 2079 2479 2793
Size: 904 1175 400 314
Fragments arranged by size:
1175 904 400 314
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2175 2209 2793
Size: 2175 34 584
Fragments arranged by size:
2175 584 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 520 2793
Size: 520 2273
BglI GCCn_nnn'nGGC
Cuts at: 0 1147 2793
Size: 1147 1646
BlpI GC'TnA_GC
Cuts at: 0 1654 2793
Size: 1654 1139
Bme1580I G_kGCm'C
Cuts at: 0 2268 2793
Size: 2268 525
BmrI ACTGGGnnnn_n'
Cuts at: 0 645 1082 1534 2782 2793
Size: 645 437 452 1248 11
Fragments arranged by size:
1248 645 452 437 11
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 570 1825 1975 2793
Size: 570 1255 150 818
Fragments arranged by size:
1255 818 570 150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 2542 2793
Size: 227 2315 251
Fragments arranged by size:
2315 251 227
Bpu10I CC'TnA_GC
Cuts at: 0 1943 2793
Size: 1943 850
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 994 1566 2793
Size: 994 572 1227
Fragments arranged by size:
1227 994 572
BsaI GGTCTCn'nnnn_
Cuts at: 0 253 2586 2793
Size: 253 2333 207
Fragments arranged by size:
2333 253 207
BsaAI yAC'GTr
Cuts at: 0 2177 2793
Size: 2177 616
BsaBI GATnn'nnATC
Cuts at: 0 205 2793
Size: 205 2588
BsaHI Gr'CG_yC
Cuts at: 0 2021 2793
Size: 2021 772
BsaWI w'CCGG_w
Cuts at: 0 1363 1661 2793
Size: 1363 298 1132
Fragments arranged by size:
1363 1132 298
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 147 177 1306 1336 1814 1844 2793
Size: 147 30 1129 30 478 30 949
Fragments arranged by size:
1129 949 478 147 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 42 1361 1564 1567 1964 2793
Size: 42 1319 203 3 397 829
Fragments arranged by size:
1319 829 397 203 42 3
BseYI C'CCAG_C
Cuts at: 0 756 1102 1422 2250 2793
Size: 756 346 320 828 543
Fragments arranged by size:
828 756 543 346 320
BsiEI CG_ry'CG
Cuts at: 0 1710 2049 2793
Size: 1710 339 744
Fragments arranged by size:
1710 744 339
BsiHKAI G_wGCw'C
Cuts at: 0 1101 1271 2793
Size: 1101 170 1522
Fragments arranged by size:
1522 1101 170
BsmI GAATG_Cn'
Cuts at: 0 2186 2793
Size: 2186 607
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1018 2686 2793
Size: 1018 1668 107
Fragments arranged by size:
1668 1018 107
Bsp1286I G_dGCh'C
Cuts at: 0 958 1077 1101 1271 1382 2268 2793
Size: 958 119 24 170 111 886 525
Fragments arranged by size:
958 886 525 170 119 111 24
BspEI T'CCGG_A
Cuts at: 0 1363 2793
Size: 1363 1430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1947 2793
Size: 1947 846
BsrBI CCG'CTC
Cuts at: 0 241 1322 2793
Size: 241 1081 1471
Fragments arranged by size:
1471 1081 241
BsrFI r'CCGG_y
Cuts at: 0 1661 2793
Size: 1661 1132
BssSI C'ACGA_G
Cuts at: 0 1270 1375 2793
Size: 1270 105 1418
Fragments arranged by size:
1418 1270 105
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1471 2793
Size: 1471 1322
BstEII G'GTnAC_C
Cuts at: 0 324 1533 2317 2793
Size: 324 1209 784 476
Fragments arranged by size:
1209 784 476 324
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1176 1296 2793
Size: 1176 120 1497
Fragments arranged by size:
1497 1176 120
BstYI r'GATC_y
Cuts at: 0 2525 2640 2793
Size: 2525 115 153
Fragments arranged by size:
2525 153 115
BtgI C'CryG_G
Cuts at: 0 577 1175 1326 2560 2793
Size: 577 598 151 1234 233
Fragments arranged by size:
1234 598 577 233 151
BtsI GCAGTG_nn'
Cuts at: 0 967 1073 2000 2793
Size: 967 106 927 793
Fragments arranged by size:
967 927 793 106
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 828 863 954 989 2132 2167 2793
Size: 828 35 91 35 1143 35 626
Fragments arranged by size:
1143 828 626 91 35 35 35
DraI TTT'AAA
Cuts at: 0 720 2793
Size: 720 2073
DraIII CAC_nnn'GTG
Cuts at: 0 526 2793
Size: 526 2267
DrdI GACnn_nn'nnGTC
Cuts at: 0 906 1759 2793
Size: 906 853 1034
Fragments arranged by size:
1034 906 853
EaeI y'GGCC_r
Cuts at: 0 2 468 741 782 1127 1707 1721 2696
Size: 2 466 273 41 345 580 14 975
Cuts at: 2696 2793
Size: 97
Fragments arranged by size:
975 580 466 345 273 97 41 14
2
EagI C'GGCC_G
Cuts at: 0 1707 2793
Size: 1707 1086
EarI CTCTTCn'nnn_
Cuts at: 0 40 946 2793
Size: 40 906 1847
Fragments arranged by size:
1847 906 40
EcoICRI GAG'CTC
Cuts at: 0 1269 2793
Size: 1269 1524
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 637 782 911 1217 2542 2743 2793
Size: 227 410 145 129 306 1325 201 50
Fragments arranged by size:
1325 410 306 227 201 145 129 50
EcoNI CCTnn'n_nnAGG
Cuts at: 0 88 2793
Size: 88 2705
EcoO109I rG'GnC_Cy
Cuts at: 0 1030 1149 1586 2793
Size: 1030 119 437 1207
Fragments arranged by size:
1207 1030 437 119
EcoRI G'AATT_C
Cuts at: 0 1006 1342 2793
Size: 1006 336 1451
Fragments arranged by size:
1451 1006 336
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2724 2793
Size: 2724 69
FspI TGC'GCA
Cuts at: 0 1434 2793
Size: 1434 1359
HaeII r_GCGC'y
Cuts at: 0 2024 2793
Size: 2024 769
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 147 179 874 906 1599 1631 1814 1846
Size: 147 32 695 32 693 32 183 32
Cuts at: 1846 1856 1888 2793
Size: 10 32 905
Fragments arranged by size:
905 695 693 183 147 32 32 32
32 32 10
HincII GTy'rAC
Cuts at: 0 456 1356 2273 2418 2493 2793
Size: 456 900 917 145 75 300
Fragments arranged by size:
917 900 456 300 145 75
KasI G'GCGC_C
Cuts at: 0 2020 2793
Size: 2020 773
KpnI G_GTAC'C
Cuts at: 0 2540 2793
Size: 2540 253
MscI TGG'CCA
Cuts at: 0 470 784 2793
Size: 470 314 2009
Fragments arranged by size:
2009 470 314
MslI CAynn'nnrTG
Cuts at: 0 428 547 1732 2122 2793
Size: 428 119 1185 390 671
Fragments arranged by size:
1185 671 428 390 119
MspA1I CmG'CkG
Cuts at: 0 247 615 632 760 2793
Size: 247 368 17 128 2033
Fragments arranged by size:
2033 368 247 128 17
NarI GG'CG_CC
Cuts at: 0 2021 2793
Size: 2021 772
NcoI C'CATG_G
Cuts at: 0 1175 1326 2793
Size: 1175 151 1467
Fragments arranged by size:
1467 1175 151
NdeI CA'TA_TG
Cuts at: 0 169 2793
Size: 169 2624
PfoI T'CCnGG_A
Cuts at: 0 1858 2467 2528 2793
Size: 1858 609 61 265
Fragments arranged by size:
1858 609 265 61
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1369 1401 2793
Size: 1369 32 1392
Fragments arranged by size:
1392 1369 32
PpuMI rG'GwC_Cy
Cuts at: 0 1586 2793
Size: 1586 1207
PshAI GACnn'nnGTC
Cuts at: 0 2488 2793
Size: 2488 305
PstI C_TGCA'G
Cuts at: 0 906 2044 2288 2793
Size: 906 1138 244 505
Fragments arranged by size:
1138 906 505 244
PvuII CAG'CTG
Cuts at: 0 247 615 760 2793
Size: 247 368 145 2033
Fragments arranged by size:
2033 368 247 145
SacI G_AGCT'C
Cuts at: 0 1271 2793
Size: 1271 1522
SalI G'TCGA_C
Cuts at: 0 1354 2793
Size: 1354 1439
SapI GCTCTTCn'nnn_
Cuts at: 0 946 2793
Size: 946 1847
SexAI A'CCwGG_T
Cuts at: 0 522 2793
Size: 522 2271
SfcI C'TryA_G
Cuts at: 0 144 561 902 1884 2040 2220 2284 2793
Size: 144 417 341 982 156 180 64 509
Fragments arranged by size:
982 509 417 341 180 156 144 64
SfoI GGC'GCC
Cuts at: 0 2022 2793
Size: 2022 771
SgrAI Cr'CCGG_yG
Cuts at: 0 1661 2793
Size: 1661 1132
SmaI CCC'GGG
Cuts at: 0 2578 2793
Size: 2578 215
SmlI C'TyrA_G
Cuts at: 0 973 1581 2793
Size: 973 608 1212
Fragments arranged by size:
1212 973 608
SspI AAT'ATT
Cuts at: 0 78 2793
Size: 78 2715
StuI AGG'CCT
Cuts at: 0 2720 2793
Size: 2720 73
StyI C'CwwG_G
Cuts at: 0 89 839 1175 1326 2120 2331 2426 2793
Size: 89 750 336 151 794 211 95 367
Fragments arranged by size:
794 750 367 336 211 151 95 89
SwaI ATTT'AAAT
Cuts at: 0 720 2793
Size: 720 2073
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1288 1747 2793
Size: 1288 459 1046
Fragments arranged by size:
1288 1046 459
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1776 1808 2793
Size: 1776 32 985
Fragments arranged by size:
1776 985 32
Tth111I GACn'n_nGTC
Cuts at: 0 1977 2793
Size: 1977 816
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 336 1110 1402 2428 2547 2793
Size: 336 774 292 1026 119 246
Fragments arranged by size:
1026 774 336 292 246 119
XmaI C'CCGG_G
Cuts at: 0 2576 2793
Size: 2576 217
XmnI GAAnn'nnTTC
Cuts at: 0 2707 2793
Size: 2707 86
Enzymes that do cut:
AccI Acc65I AcuI AgeI AleI AloI ApoI AvaI
BanI BanII BbeI BbsI BcgI BciVI BglI BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssSI
BstAPI BstEII BstXI BstYI BtgI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MscI MslI MspA1I NarI NcoI NdeI PfoI
PpiI PpuMI PshAI PstI PvuII SacI SalI SapI
SexAI SfcI SfoI SgrAI SmaI SmlI SspI StuI
StyI SwaI TaqII TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AhdI AlfI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BclI BfrBI BglII BmgBI BmtI BsgI
BsiWI BspHI BsrDI BsrGI BssHII BstBI BstZ17I Bsu36I
BtgZI ClaI EciI EcoRV FseI FspAI HindIII HpaI
MfeI MluI MmeI NaeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SanDI
SbfI ScaI SfiI SgfI SnaBI SpeI SphI SrfI
TatI XbaI XhoI ZraI