Restriction map for
pFN21AE2696
(Linear) (Six-base) MAPSORT of: KIEE2696.seq Check: 8141 from: 1 to: 921
KIEE2696.orf
With 182 enzymes: SgfI *
June 29, 2017 17:53 ..
AatII G_ACGT'C
Cuts at: 0 122 921
Size: 122 799
AccI GT'mk_AC
Cuts at: 0 398 921
Size: 398 523
Acc65I G'GTAC_C
Cuts at: 0 305 921
Size: 305 616
AleI CACnn'nnGTG
Cuts at: 0 475 921
Size: 475 446
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 740 921
Size: 740 181
ApaLI G'TGCA_C
Cuts at: 0 592 921
Size: 592 329
BanI G'GyrC_C
Cuts at: 0 305 921
Size: 305 616
BbsI GAAGACnn'nnnn_
Cuts at: 0 361 921
Size: 361 560
BbvCI CC'TCA_GC
Cuts at: 0 310 921
Size: 310 611
BlpI GC'TnA_GC
Cuts at: 0 220 921
Size: 220 701
Bme1580I G_kGCm'C
Cuts at: 0 596 921
Size: 596 325
BmgBI CAC'GTC
Cuts at: 0 237 374 921
Size: 237 137 547
Fragments arranged by size:
547 237 137
BmrI ACTGGGnnnn_n'
Cuts at: 0 282 921
Size: 282 639
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 232 921
Size: 94 138 689
Fragments arranged by size:
689 138 94
Bpu10I CC'TnA_GC
Cuts at: 0 310 389 921
Size: 310 79 532
Fragments arranged by size:
532 310 79
BsaI GGTCTCn'nnnn_
Cuts at: 0 434 921
Size: 434 487
BsaAI yAC'GTr
Cuts at: 0 591 921
Size: 591 330
BsaHI Gr'CG_yC
Cuts at: 0 119 921
Size: 119 802
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 292 538 921
Size: 292 246 383
Fragments arranged by size:
383 292 246
BseYI C'CCAG_C
Cuts at: 0 38 44 152 921
Size: 38 6 108 769
Fragments arranged by size:
769 108 38 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 838 921
Size: 211 627 83
Fragments arranged by size:
627 211 83
BsiEI CG_ry'CG
Cuts at: 0 245 654 783 921
Size: 245 409 129 138
Fragments arranged by size:
409 245 138 129
BsiHKAI G_wGCw'C
Cuts at: 0 596 921
Size: 596 325
Bsp1286I G_dGCh'C
Cuts at: 0 596 921
Size: 596 325
BspHI T'CATG_A
Cuts at: 0 867 921
Size: 867 54
BssHII G'CGCG_C
Cuts at: 0 201 921
Size: 201 720
BstBI TT'CG_AA
Cuts at: 0 730 921
Size: 730 191
BstYI r'GATC_y
Cuts at: 0 680 921
Size: 680 241
BtgI C'CryG_G
Cuts at: 0 245 291 921
Size: 245 46 630
Fragments arranged by size:
630 245 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 140 554 921
Size: 140 414 367
Fragments arranged by size:
414 367 140
BtsI GCAGTG_nn'
Cuts at: 0 205 921
Size: 205 716
DraIII CAC_nnn'GTG
Cuts at: 0 642 921
Size: 642 279
EaeI y'GGCC_r
Cuts at: 0 2 242 331 780 921
Size: 2 240 89 449 141
Fragments arranged by size:
449 240 141 89 2
EagI C'GGCC_G
Cuts at: 0 242 780 921
Size: 242 538 141
Fragments arranged by size:
538 242 141
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 232 921
Size: 94 138 689
Fragments arranged by size:
689 138 94
EcoO109I rG'GnC_Cy
Cuts at: 0 104 567 666 921
Size: 104 463 99 255
Fragments arranged by size:
463 255 104 99
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 903 921
Size: 903 18
FspI TGC'GCA
Cuts at: 0 187 921
Size: 187 734
KpnI G_GTAC'C
Cuts at: 0 309 921
Size: 309 612
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 921
Size: 428 493
MscI TGG'CCA
Cuts at: 0 333 921
Size: 333 588
MslI CAynn'nnrTG
Cuts at: 0 181 475 921
Size: 181 294 446
Fragments arranged by size:
446 294 181
MspA1I CmG'CkG
Cuts at: 0 247 624 779 791 921
Size: 247 377 155 12 130
Fragments arranged by size:
377 247 155 130 12
NotI GC'GGCC_GC
Cuts at: 0 242 921
Size: 242 679
NspI r_CATG'y
Cuts at: 0 495 600 921
Size: 495 105 321
Fragments arranged by size:
495 321 105
PflMI CCAn_nnn'nTGG
Cuts at: 0 297 642 921
Size: 297 345 279
Fragments arranged by size:
345 297 279
PfoI T'CCnGG_A
Cuts at: 0 110 921
Size: 110 811
PmlI CAC'GTG
Cuts at: 0 591 921
Size: 591 330
PpuMI rG'GwC_Cy
Cuts at: 0 104 666 921
Size: 104 562 255
Fragments arranged by size:
562 255 104
SacII CC_GC'GG
Cuts at: 0 248 921
Size: 248 673
SanDI GG'GwC_CC
Cuts at: 0 104 666 921
Size: 104 562 255
Fragments arranged by size:
562 255 104
SfcI C'TryA_G
Cuts at: 0 399 921
Size: 399 522
SspI AAT'ATT
Cuts at: 0 862 921
Size: 862 59
StyI C'CwwG_G
Cuts at: 0 752 921
Size: 752 169
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 487 921
Size: 487 434
XmnI GAAnn'nnTTC
Cuts at: 0 863 886 921
Size: 863 23 35
Fragments arranged by size:
863 35 23
ZraI GAC'GTC
Cuts at: 0 120 921
Size: 120 801
Enzymes that do cut:
AatII AccI Acc65I AleI AlfI ApaLI BanI BbsI
BbvCI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI
BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BssHII BstBI BstYI BtgI BtgZI BtsI DraIII
EaeI EagI Eco57MI EcoO109I FalI FspI KpnI MmeI
MscI MslI MspA1I NotI NspI PflMI PfoI PmlI
PpuMI SacII SanDI SfcI SspI StyI TaqII XmnI
ZraI
Enzymes that do not cut:
AarI AclI AcuI AfeI AflII AflIII AgeI AhdI
AloI AlwNI ApaI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanII BbeI BcgI BciVI BclI
BfrBI BglI BglII BmtI BplI BpuEI BsaBI BsaWI
BsaXI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraI DrdI EarI EciI EcoICRI EcoNI
EcoRI EcoRV FseI FspAI HaeII Hin4I HincII HindIII
HpaI KasI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NruI NsiI PacI PasI PciI PmeI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI StuI SwaI TatI TstI Tth111I XbaI
XcmI XhoI XmaI