Restriction map for
pFN21AE2687
(Linear) (Six-base) MAPSORT of: KIEE2687.seq Check: 9269 from: 1 to: 1737
KIEE2687.orf
With 182 enzymes: SgfI *
May 19, 2017 16:27 ..
AccI GT'mk_AC
Cuts at: 0 953 1737
Size: 953 784
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 817 924 1125 1243 1737
Size: 541 276 107 201 118 494
Fragments arranged by size:
541 494 276 201 118 107
AfeI AGC'GCT
Cuts at: 0 181 1737
Size: 181 1556
AflIII A'CryG_T
Cuts at: 0 902 1737
Size: 902 835
AleI CACnn'nnGTG
Cuts at: 0 298 1531 1737
Size: 298 1233 206
Fragments arranged by size:
1233 298 206
AlwNI CAG_nnn'CTG
Cuts at: 0 396 972 1737
Size: 396 576 765
Fragments arranged by size:
765 576 396
ApaI G_GGCC'C
Cuts at: 0 669 844 1737
Size: 669 175 893
Fragments arranged by size:
893 669 175
ApaLI G'TGCA_C
Cuts at: 0 547 1737
Size: 547 1190
AvaI C'yCGr_G
Cuts at: 0 851 1737
Size: 851 886
BanI G'GyrC_C
Cuts at: 0 34 98 483 532 1518 1737
Size: 34 64 385 49 986 219
Fragments arranged by size:
986 385 219 64 49 34
BanII G_rGCy'C
Cuts at: 0 649 669 844 888 1139 1737
Size: 649 20 175 44 251 598
Fragments arranged by size:
649 598 251 175 44 20
BbeI G_GCGC'C
Cuts at: 0 38 102 1737
Size: 38 64 1635
Fragments arranged by size:
1635 64 38
BbvCI CC'TCA_GC
Cuts at: 0 239 781 1297 1541 1737
Size: 239 542 516 244 196
Fragments arranged by size:
542 516 244 239 196
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 693 727 1737
Size: 693 34 1010
Fragments arranged by size:
1010 693 34
BglI GCCn_nnn'nGGC
Cuts at: 0 280 644 800 1465 1737
Size: 280 364 156 665 272
Fragments arranged by size:
665 364 280 272 156
Bme1580I G_kGCm'C
Cuts at: 0 542 551 669 844 1073 1737
Size: 542 9 118 175 229 664
Fragments arranged by size:
664 542 229 175 118 9
BmgBI CAC'GTC
Cuts at: 0 858 1737
Size: 858 879
BmrI ACTGGGnnnn_n'
Cuts at: 0 1374 1737
Size: 1374 363
BmtI G_CTAG'C
Cuts at: 0 226 1737
Size: 226 1511
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 701 1060 1135 1737
Size: 175 526 359 75 602
Fragments arranged by size:
602 526 359 175 75
Bpu10I CC'TnA_GC
Cuts at: 0 239 699 781 1297 1541 1737
Size: 239 460 82 516 244 196
Fragments arranged by size:
516 460 244 239 196 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 486 745 1078 1737
Size: 406 80 259 333 659
Fragments arranged by size:
659 406 333 259 80
BsaI GGTCTCn'nnnn_
Cuts at: 0 1036 1111 1218 1737
Size: 1036 75 107 519
Fragments arranged by size:
1036 519 107 75
BsaHI Gr'CG_yC
Cuts at: 0 35 99 407 1737
Size: 35 64 308 1330
Fragments arranged by size:
1330 308 64 35
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 912 942 1108 1138 1737
Size: 912 30 166 30 599
Fragments arranged by size:
912 599 166 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 639 726 1737
Size: 639 87 1011
Fragments arranged by size:
1011 639 87
BseYI C'CCAG_C
Cuts at: 0 459 1737
Size: 459 1278
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 268 1666 1737
Size: 25 243 1398 71
Fragments arranged by size:
1398 243 71 25
BsiEI CG_ry'CG
Cuts at: 0 77 1737
Size: 77 1660
BsiHKAI G_wGCw'C
Cuts at: 0 290 551 1737
Size: 290 261 1186
Fragments arranged by size:
1186 290 261
BsmI GAATG_Cn'
Cuts at: 0 1449 1737
Size: 1449 288
BsmBI CGTCTCn'nnnn_
Cuts at: 0 835 1737
Size: 835 902
Bsp1286I G_dGCh'C
Cuts at: 0 290 542 551 649 669 844 888 1073
Size: 290 252 9 98 20 175 44 185
Cuts at: 1073 1139 1737
Size: 66 598
Fragments arranged by size:
598 290 252 185 175 98 66 44
20 9
BsrBI CCG'CTC
Cuts at: 0 148 1737
Size: 148 1589
BsrFI r'CCGG_y
Cuts at: 0 486 1635 1737
Size: 486 1149 102
Fragments arranged by size:
1149 486 102
BsrGI T'GTAC_A
Cuts at: 0 1487 1737
Size: 1487 250
BssSI C'ACGA_G
Cuts at: 0 1345 1737
Size: 1345 392
BstAPI GCAn_nnn'nTGC
Cuts at: 0 396 1737
Size: 396 1341
Bsu36I CC'TnA_GG
Cuts at: 0 278 651 1737
Size: 278 373 1086
Fragments arranged by size:
1086 373 278
BtgI C'CryG_G
Cuts at: 0 77 619 836 845 1529 1737
Size: 77 542 217 9 684 208
Fragments arranged by size:
684 542 217 208 77 9
BtsI GCAGTG_nn'
Cuts at: 0 830 1654 1737
Size: 830 824 83
Fragments arranged by size:
830 824 83
DrdI GACnn_nn'nnGTC
Cuts at: 0 372 1737
Size: 372 1365
EaeI y'GGCC_r
Cuts at: 0 74 603 1019 1152 1640 1737
Size: 74 529 416 133 488 97
Fragments arranged by size:
529 488 416 133 97 74
EagI C'GGCC_G
Cuts at: 0 74 1737
Size: 74 1663
EarI CTCTTCn'nnn_
Cuts at: 0 295 941 1144 1615 1667 1737
Size: 295 646 203 471 52 70
Fragments arranged by size:
646 471 295 203 70 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 505 1737
Size: 505 1232
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 541 701 817 924 1060 1125 1135
Size: 175 366 160 116 107 136 65 10
Cuts at: 1135 1243 1737
Size: 108 494
Fragments arranged by size:
494 366 175 160 136 116 108 107
65 10
EcoO109I rG'GnC_Cy
Cuts at: 0 393 665 666 841 1206 1737
Size: 393 272 1 175 365 531
Fragments arranged by size:
531 393 365 272 175 1
EcoRV GAT'ATC
Cuts at: 0 711 1587 1737
Size: 711 876 150
Fragments arranged by size:
876 711 150
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 447 1737
Size: 447 1290
HaeII r_GCGC'y
Cuts at: 0 38 102 183 220 1393 1737
Size: 38 64 81 37 1173 344
Fragments arranged by size:
1173 344 81 64 38 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 193 225 333 365 707 739 935 967
Size: 193 32 108 32 342 32 196 32
Cuts at: 967 1108 1140 1198 1230 1737
Size: 141 32 58 32 507
Fragments arranged by size:
507 342 196 193 141 108 58 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1127 1737
Size: 1127 610
HindIII A'AGCT_T
Cuts at: 0 1225 1737
Size: 1225 512
KasI G'GCGC_C
Cuts at: 0 34 98 1737
Size: 34 64 1639
Fragments arranged by size:
1639 64 34
MscI TGG'CCA
Cuts at: 0 605 1154 1737
Size: 605 549 583
Fragments arranged by size:
605 583 549
MslI CAynn'nnrTG
Cuts at: 0 298 554 1531 1737
Size: 298 256 977 206
Fragments arranged by size:
977 298 256 206
MspA1I CmG'CkG
Cuts at: 0 243 372 1737
Size: 243 129 1365
Fragments arranged by size:
1365 243 129
NaeI GCC'GGC
Cuts at: 0 488 1737
Size: 488 1249
NarI GG'CG_CC
Cuts at: 0 35 99 1737
Size: 35 64 1638
Fragments arranged by size:
1638 64 35
NcoI C'CATG_G
Cuts at: 0 619 845 1737
Size: 619 226 892
Fragments arranged by size:
892 619 226
NgoMIV G'CCGG_C
Cuts at: 0 486 1737
Size: 486 1251
NheI G'CTAG_C
Cuts at: 0 222 1737
Size: 222 1515
NspI r_CATG'y
Cuts at: 0 517 906 1737
Size: 517 389 831
Fragments arranged by size:
831 517 389
PasI CC'CwG_GG
Cuts at: 0 640 693 1737
Size: 640 53 1044
Fragments arranged by size:
1044 640 53
PciI A'CATG_T
Cuts at: 0 902 1737
Size: 902 835
PflMI CCAn_nnn'nTGG
Cuts at: 0 1113 1737
Size: 1113 624
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 889 921 1737
Size: 889 32 816
Fragments arranged by size:
889 816 32
PpuMI rG'GwC_Cy
Cuts at: 0 1206 1737
Size: 1206 531
PspOMI G'GGCC_C
Cuts at: 0 665 840 1737
Size: 665 175 897
Fragments arranged by size:
897 665 175
PstI C_TGCA'G
Cuts at: 0 530 589 1343 1512 1607 1737
Size: 530 59 754 169 95 130
Fragments arranged by size:
754 530 169 130 95 59
PvuII CAG'CTG
Cuts at: 0 243 372 1737
Size: 243 129 1365
Fragments arranged by size:
1365 243 129
SapI GCTCTTCn'nnn_
Cuts at: 0 295 1144 1737
Size: 295 849 593
Fragments arranged by size:
849 593 295
SbfI CC_TGCA'GG
Cuts at: 0 1512 1737
Size: 1512 225
SexAI A'CCwGG_T
Cuts at: 0 1208 1737
Size: 1208 529
SfcI C'TryA_G
Cuts at: 0 526 585 1165 1339 1508 1603 1737
Size: 526 59 580 174 169 95 134
Fragments arranged by size:
580 526 174 169 134 95 59
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 280 1465 1737
Size: 280 1185 272
Fragments arranged by size:
1185 280 272
SfoI GGC'GCC
Cuts at: 0 36 100 1737
Size: 36 64 1637
Fragments arranged by size:
1637 64 36
SmaI CCC'GGG
Cuts at: 0 853 1737
Size: 853 884
SmlI C'TyrA_G
Cuts at: 0 421 465 760 1057 1737
Size: 421 44 295 297 680
Fragments arranged by size:
680 421 297 295 44
StuI AGG'CCT
Cuts at: 0 310 1737
Size: 310 1427
StyI C'CwwG_G
Cuts at: 0 619 845 1279 1737
Size: 619 226 434 458
Fragments arranged by size:
619 458 434 226
TatI w'GTAC_w
Cuts at: 0 1487 1737
Size: 1487 250
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 321 353 543 575 912 944 1065 1097
Size: 321 32 190 32 337 32 121 32
Cuts at: 1097 1737
Size: 640
Fragments arranged by size:
640 337 321 190 121 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 1122 1210 1737
Size: 1122 88 527
Fragments arranged by size:
1122 527 88
XmaI C'CCGG_G
Cuts at: 0 851 1737
Size: 851 886
Enzymes that do cut:
AccI AcuI AfeI AflIII AleI AlwNI ApaI ApaLI
AvaI BanI BanII BbeI BbvCI BcgI BglI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BsrBI BsrFI BsrGI BssSI BstAPI Bsu36I BtgI
BtsI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRV FalI HaeII Hin4I HincII HindIII KasI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PasI PciI PflMI PpiI PpuMI PspOMI PstI PvuII
SapI SbfI SexAI SfcI SfiI SfoI SmaI SmlI
StuI StyI TatI TstI Tth111I XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AlfI
AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BciVI BclI BfrBI BglII BlpI BplI BsaAI
BsaBI BsaWI BsiWI BspEI BspHI BspMI BsrDI BssHII
BstBI BstEII BstXI BstYI BstZ17I BtgZI ClaI CspCI
DraI DraIII EcoICRI EcoNI EcoRI FseI FspI FspAI
HpaI KpnI MfeI MluI MmeI NdeI NotI NruI
NsiI PacI PfoI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI ScaI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TaqII XbaI XcmI XhoI XmnI ZraI