Restriction map for
pFN21AE2686
(Linear) (Six-base) MAPSORT of: KIEE2686.seq Check: 5495 from: 1 to: 1524
KIEE2686.orf
With 182 enzymes: SgfI *
May 11, 2017 11:17 ..
AclI AA'CG_TT
Cuts at: 0 1127 1524
Size: 1127 397
AhdI GACnn_n'nnGTC
Cuts at: 0 312 1524
Size: 312 1212
AleI CACnn'nnGTG
Cuts at: 0 925 1524
Size: 925 599
AlwNI CAG_nnn'CTG
Cuts at: 0 423 1524
Size: 423 1101
ApoI r'AATT_y
Cuts at: 0 757 1524
Size: 757 767
BanI G'GyrC_C
Cuts at: 0 62 70 205 1217 1398 1524
Size: 62 8 135 1012 181 126
Fragments arranged by size:
1012 181 135 126 62 8
BanII G_rGCy'C
Cuts at: 0 11 1355 1524
Size: 11 1344 169
Fragments arranged by size:
1344 169 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 504 1524
Size: 504 1020
BbvCI CC'TCA_GC
Cuts at: 0 1511 1524
Size: 1511 13
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 352 386 1524
Size: 352 34 1138
Fragments arranged by size:
1138 352 34
BfrBI ATG'CAT
Cuts at: 0 147 1524
Size: 147 1377
Bme1580I G_kGCm'C
Cuts at: 0 67 208 1168 1524
Size: 67 141 960 356
Fragments arranged by size:
960 356 141 67
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 1078 1119 1414 1515 1524
Size: 445 633 41 295 101 9
Fragments arranged by size:
633 445 295 101 41 9
Bpu10I CC'TnA_GC
Cuts at: 0 625 1241 1511 1524
Size: 625 616 270 13
Fragments arranged by size:
625 616 270 13
BsaI GGTCTCn'nnnn_
Cuts at: 0 907 1524
Size: 907 617
BsaBI GATnn'nnATC
Cuts at: 0 413 1524
Size: 413 1111
BsaWI w'CCGG_w
Cuts at: 0 761 1524
Size: 761 763
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1117 1147 1524
Size: 1117 30 377
Fragments arranged by size:
1117 377 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1003 1039 1524
Size: 1003 36 485
Fragments arranged by size:
1003 485 36
BseYI C'CCAG_C
Cuts at: 0 1268 1524
Size: 1268 256
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 940 1524
Size: 940 584
BsiHKAI G_wGCw'C
Cuts at: 0 11 1355 1524
Size: 11 1344 169
Fragments arranged by size:
1344 169 11
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1051 1524
Size: 1051 473
Bsp1286I G_dGCh'C
Cuts at: 0 11 67 208 1168 1355 1524
Size: 11 56 141 960 187 169
Fragments arranged by size:
960 187 169 141 56 11
BspEI T'CCGG_A
Cuts at: 0 761 1524
Size: 761 763
BsrDI GCAATG_nn'
Cuts at: 0 834 1524
Size: 834 690
BssSI C'ACGA_G
Cuts at: 0 10 1524
Size: 10 1514
BstEII G'GTnAC_C
Cuts at: 0 522 1524
Size: 522 1002
BstXI CCAn_nnnn'nTGG
Cuts at: 0 786 927 1524
Size: 786 141 597
Fragments arranged by size:
786 597 141
BstYI r'GATC_y
Cuts at: 0 1334 1524
Size: 1334 190
Bsu36I CC'TnA_GG
Cuts at: 0 965 1087 1524
Size: 965 122 437
Fragments arranged by size:
965 437 122
BtgI C'CryG_G
Cuts at: 0 1094 1524
Size: 1094 430
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 294 397 1524
Size: 294 103 1127
Fragments arranged by size:
1127 294 103
EaeI y'GGCC_r
Cuts at: 0 138 228 242 386 470 1524
Size: 138 90 14 144 84 1054
Fragments arranged by size:
1054 144 138 90 84 14
EarI CTCTTCn'nnn_
Cuts at: 0 1360 1524
Size: 1360 164
EcoICRI GAG'CTC
Cuts at: 0 9 1353 1524
Size: 9 1344 171
Fragments arranged by size:
1344 171 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 1078 1119 1414 1515 1524
Size: 445 633 41 295 101 9
Fragments arranged by size:
633 445 295 101 41 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 403 1057 1524
Size: 403 654 467
Fragments arranged by size:
654 467 403
EcoO109I rG'GnC_Cy
Cuts at: 0 573 1265 1312 1524
Size: 573 692 47 212
Fragments arranged by size:
692 573 212 47
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1464 1524
Size: 1464 60
HaeII r_GCGC'y
Cuts at: 0 1195 1524
Size: 1195 329
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 298 330 423 455 832 864 1524
Size: 298 32 93 32 377 32 660
Fragments arranged by size:
660 377 298 93 32 32 32
HincII GTy'rAC
Cuts at: 0 1131 1524
Size: 1131 393
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 1524
Size: 341 1183
MscI TGG'CCA
Cuts at: 0 244 388 472 1524
Size: 244 144 84 1052
Fragments arranged by size:
1052 244 144 84
MslI CAynn'nnrTG
Cuts at: 0 781 796 925 1202 1524
Size: 781 15 129 277 322
Fragments arranged by size:
781 322 277 129 15
MspA1I CmG'CkG
Cuts at: 0 240 366 1261 1416 1524
Size: 240 126 895 155 108
Fragments arranged by size:
895 240 155 126 108
NdeI CA'TA_TG
Cuts at: 0 143 1524
Size: 143 1381
NsiI A_TGCA'T
Cuts at: 0 149 1524
Size: 149 1375
NspI r_CATG'y
Cuts at: 0 786 1524
Size: 786 738
PflMI CCAn_nnn'nTGG
Cuts at: 0 240 1005 1408 1524
Size: 240 765 403 116
Fragments arranged by size:
765 403 240 116
PpuMI rG'GwC_Cy
Cuts at: 0 573 1265 1312 1524
Size: 573 692 47 212
Fragments arranged by size:
692 573 212 47
PstI C_TGCA'G
Cuts at: 0 170 1382 1524
Size: 170 1212 142
Fragments arranged by size:
1212 170 142
PvuII CAG'CTG
Cuts at: 0 240 366 1416 1524
Size: 240 126 1050 108
Fragments arranged by size:
1050 240 126 108
SacI G_AGCT'C
Cuts at: 0 11 1355 1524
Size: 11 1344 169
Fragments arranged by size:
1344 169 11
SanDI GG'GwC_CC
Cuts at: 0 573 1265 1524
Size: 573 692 259
Fragments arranged by size:
692 573 259
SapI GCTCTTCn'nnn_
Cuts at: 0 1360 1524
Size: 1360 164
SfcI C'TryA_G
Cuts at: 0 166 215 1378 1524
Size: 166 49 1163 146
Fragments arranged by size:
1163 166 146 49
StuI AGG'CCT
Cuts at: 0 1492 1524
Size: 1492 32
StyI C'CwwG_G
Cuts at: 0 245 1439 1524
Size: 245 1194 85
Fragments arranged by size:
1194 245 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 490 1524
Size: 490 1034
TatI w'GTAC_w
Cuts at: 0 451 1524
Size: 451 1073
Tth111I GACn'n_nGTC
Cuts at: 0 649 1524
Size: 649 875
XbaI T'CTAG_A
Cuts at: 0 303 1524
Size: 303 1221
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 681 924 1524
Size: 681 243 600
Fragments arranged by size:
681 600 243
XmnI GAAnn'nnTTC
Cuts at: 0 667 1524
Size: 667 857
Enzymes that do cut:
AclI AhdI AleI AlwNI ApoI BanI BanII BbsI
BbvCI BcgI BfrBI Bme1580I BpmI Bpu10I BsaI BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspEI BsrDI BssSI BstEII BstXI BstYI Bsu36I BtgI
BtgZI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HincII MmeI MscI MslI MspA1I NdeI
NsiI NspI PflMI PpuMI PstI PvuII SacI SanDI
SapI SfcI StuI StyI TaqII TatI Tth111I XbaI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AflIII
AgeI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BciVI BclI BglI
BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaAI
BsaHI BsiEI BsiWI BsmI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BstAPI BstBI BstZ17I BtsI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoRI EcoRV FseI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NgoMIV NheI NotI NruI PacI
PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TstI XhoI
XmaI ZraI