Restriction map for
pFN21AE2683
(Linear) (Six-base) MAPSORT of: KIEE2683.seq Check: 6782 from: 1 to: 3069
KIEE2683.orf
With 182 enzymes: SgfI *
March 22, 2017 11:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 175 2009 3069
Size: 175 1834 1060
Fragments arranged by size:
1834 1060 175
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 2598 2773 3069
Size: 332 2266 175 296
Fragments arranged by size:
2266 332 296 175
AgeI A'CCGG_T
Cuts at: 0 2843 3069
Size: 2843 226
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 122 690 3069
Size: 122 568 2379
Fragments arranged by size:
2379 568 122
AlwNI CAG_nnn'CTG
Cuts at: 0 208 442 1948 2225 3063 3069
Size: 208 234 1506 277 838 6
Fragments arranged by size:
1506 838 277 234 208 6
ApaI G_GGCC'C
Cuts at: 0 1214 1462 3069
Size: 1214 248 1607
Fragments arranged by size:
1607 1214 248
AvaI C'yCGr_G
Cuts at: 0 762 791 2744 3069
Size: 762 29 1953 325
Fragments arranged by size:
1953 762 325 29
BamHI G'GATC_C
Cuts at: 0 1851 3069
Size: 1851 1218
BanI G'GyrC_C
Cuts at: 0 188 536 826 989 1715 2176 2236 3069
Size: 188 348 290 163 726 461 60 833
Fragments arranged by size:
833 726 461 348 290 188 163 60
BanII G_rGCy'C
Cuts at: 0 41 245 287 458 769 1214 1414 1462
Size: 41 204 42 171 311 445 200 48
Cuts at: 1462 1505 1642 1793 1991 2356 2518 2743 2909
Size: 43 137 151 198 365 162 225 166
Cuts at: 2909 2936 3069
Size: 27 133
Fragments arranged by size:
445 365 311 225 204 200 198 171
166 162 151 137 133 48 43 42
41 27
BbeI G_GCGC'C
Cuts at: 0 540 2240 3069
Size: 540 1700 829
Fragments arranged by size:
1700 829 540
BbsI GAAGACnn'nnnn_
Cuts at: 0 851 1301 2222 2339 2384 3069
Size: 851 450 921 117 45 685
Fragments arranged by size:
921 851 685 450 117 45
BbvCI CC'TCA_GC
Cuts at: 0 375 2125 2189 3069
Size: 375 1750 64 880
Fragments arranged by size:
1750 880 375 64
BglI GCCn_nnn'nGGC
Cuts at: 0 277 545 1009 2032 2185 3069
Size: 277 268 464 1023 153 884
Fragments arranged by size:
1023 884 464 277 268 153
BglII A'GATC_T
Cuts at: 0 553 3069
Size: 553 2516
BlpI GC'TnA_GC
Cuts at: 0 2920 3069
Size: 2920 149
Bme1580I G_kGCm'C
Cuts at: 0 193 831 1214 1423 1462 2181 3069
Size: 193 638 383 209 39 719 888
Fragments arranged by size:
888 719 638 383 209 193 39
BmgBI CAC'GTC
Cuts at: 0 321 1050 3069
Size: 321 729 2019
Fragments arranged by size:
2019 729 321
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 60 444 1941 3069
Size: 60 384 1497 1128
Fragments arranged by size:
1497 1128 384 60
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 764 1261 1426 1654 2332 2344 2712
Size: 694 70 497 165 228 678 12 368
Cuts at: 2712 3069
Size: 357
Fragments arranged by size:
694 678 497 368 357 228 165 70
12
Bpu10I CC'TnA_GC
Cuts at: 0 375 926 1451 2125 2189 3069
Size: 375 551 525 674 64 880
Fragments arranged by size:
880 674 551 525 375 64
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1028 1915 2176 2544 3069
Size: 1028 887 261 368 525
Fragments arranged by size:
1028 887 525 368 261
BsaI GGTCTCn'nnnn_
Cuts at: 0 2063 2261 2308 3069
Size: 2063 198 47 761
Fragments arranged by size:
2063 761 198 47
BsaHI Gr'CG_yC
Cuts at: 0 537 2105 2237 3069
Size: 537 1568 132 832
Fragments arranged by size:
1568 832 537 132
BsaWI w'CCGG_w
Cuts at: 0 2843 3069
Size: 2843 226
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 169 199 692 722 1333 1363 2326 2356
Size: 169 30 493 30 611 30 963 30
Cuts at: 2356 2725 2755 2893 2923 3069
Size: 369 30 138 30 146
Fragments arranged by size:
963 611 493 369 169 146 138 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 33 109 466 1189 1303 1324 1327 1351
Size: 33 76 357 723 114 21 3 24
Cuts at: 1351 1354 1357 1360 1861 2341 2434 2455 2458
Size: 3 3 3 501 480 93 21 3
Cuts at: 2458 2461 2464 2800 3069
Size: 3 3 336 269
Fragments arranged by size:
723 501 480 357 336 269 114 93
76 33 24 21 21 3 3 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 134 339 406 435 770 959 1392 1455
Size: 134 205 67 29 335 189 433 63
Cuts at: 1455 1563 1678 2018 2168 3053 3069
Size: 108 115 340 150 885 16
Fragments arranged by size:
885 433 340 335 205 189 150 134
115 108 67 63 29 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 3069
Size: 383 2686
BsiHKAI G_wGCw'C
Cuts at: 0 41 245 287 458 1055 1207 1414 1515
Size: 41 204 42 171 597 152 207 101
Cuts at: 1515 1793 1991 2093 2743 2858 3069
Size: 278 198 102 650 115 211
Fragments arranged by size:
650 597 278 211 207 204 198 171
152 115 102 101 42 41
BsmBI CGTCTCn'nnnn_
Cuts at: 0 350 3069
Size: 350 2719
Bsp1286I G_dGCh'C
Cuts at: 0 41 193 245 287 458 769 831 1055
Size: 41 152 52 42 171 311 62 224
Cuts at: 1055 1207 1214 1414 1423 1462 1505 1515 1642
Size: 152 7 200 9 39 43 10 127
Cuts at: 1642 1793 1991 2093 2181 2356 2518 2743 2858
Size: 151 198 102 88 175 162 225 115
Cuts at: 2858 2909 2936 3069
Size: 51 27 133
Fragments arranged by size:
311 225 224 200 198 175 171 162
152 152 151 133 127 115 102 88
62 52 51 43 42 41 39 27
10 9 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 175 954 2009 3069
Size: 175 779 1055 1060
Fragments arranged by size:
1060 1055 779 175
BsrBI CCG'CTC
Cuts at: 0 1557 2558 3069
Size: 1557 1001 511
Fragments arranged by size:
1557 1001 511
BsrFI r'CCGG_y
Cuts at: 0 1262 2534 2843 3069
Size: 1262 1272 309 226
Fragments arranged by size:
1272 1262 309 226
BsrGI T'GTAC_A
Cuts at: 0 637 2711 3069
Size: 637 2074 358
Fragments arranged by size:
2074 637 358
BssHII G'CGCG_C
Cuts at: 0 20 3069
Size: 20 3049
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3063 3069
Size: 3063 6
BstEII G'GTnAC_C
Cuts at: 0 2591 3069
Size: 2591 478
BstXI CCAn_nnnn'nTGG
Cuts at: 0 297 3069
Size: 297 2772
BstYI r'GATC_y
Cuts at: 0 553 970 1622 1851 2276 3069
Size: 553 417 652 229 425 793
Fragments arranged by size:
793 652 553 425 417 229
Bsu36I CC'TnA_GG
Cuts at: 0 443 1916 2890 3069
Size: 443 1473 974 179
Fragments arranged by size:
1473 974 443 179
BtgI C'CryG_G
Cuts at: 0 1381 1574 3023 3069
Size: 1381 193 1449 46
Fragments arranged by size:
1449 1381 193 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 871 3069
Size: 871 2198
BtsI GCAGTG_nn'
Cuts at: 0 190 460 1231 2499 3069
Size: 190 270 771 1268 570
Fragments arranged by size:
1268 771 570 270 190
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 153 188 3069
Size: 153 35 2881
Fragments arranged by size:
2881 153 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 2476 3069
Size: 2476 593
EaeI y'GGCC_r
Cuts at: 0 161 482 1082 1590 2037 3069
Size: 161 321 600 508 447 1032
Fragments arranged by size:
1032 600 508 447 321 161
EarI CTCTTCn'nnn_
Cuts at: 0 29 250 1301 1313 1756 2420 2459 2577
Size: 29 221 1051 12 443 664 39 118
Cuts at: 2577 2749 2978 3069
Size: 172 229 91
Fragments arranged by size:
1051 664 443 229 221 172 118 91
39 29 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 598 1249 3069
Size: 598 651 1820
Fragments arranged by size:
1820 651 598
EcoICRI GAG'CTC
Cuts at: 0 39 243 285 456 1412 1791 1989 2741
Size: 39 204 42 171 956 379 198 752
Cuts at: 2741 3069
Size: 328
Fragments arranged by size:
956 752 379 328 204 198 171 42
39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 694 764 1261 1426 1654 2332 2344
Size: 332 362 70 497 165 228 678 12
Cuts at: 2344 2598 2712 2773 3069
Size: 254 114 61 296
Fragments arranged by size:
678 497 362 332 296 254 228 165
114 70 61 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2326 2416 2999 3069
Size: 2326 90 583 70
Fragments arranged by size:
2326 583 90 70
EcoO109I rG'GnC_Cy
Cuts at: 0 270 1127 1459 1615 1674 1920 2129 2199
Size: 270 857 332 156 59 246 209 70
Cuts at: 2199 2988 3069
Size: 789 81
Fragments arranged by size:
857 789 332 270 246 209 156 81
70 59
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1782 2067 2778 2847 3007 3069
Size: 1782 285 711 69 160 62
Fragments arranged by size:
1782 711 285 160 69 62
HaeII r_GCGC'y
Cuts at: 0 540 2240 2763 2885 3069
Size: 540 1700 523 122 184
Fragments arranged by size:
1700 540 523 184 122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 142 174 349 381 442 474 606 638
Size: 142 32 175 32 61 32 132 32
Cuts at: 638 1607 1639 1978 2010 2326 2358 2725 2757
Size: 969 32 339 32 316 32 367 32
Cuts at: 2757 3069
Size: 312
Fragments arranged by size:
969 367 339 316 312 175 142 132
61 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 565 3069
Size: 565 2504
KasI G'GCGC_C
Cuts at: 0 536 2236 3069
Size: 536 1700 833
Fragments arranged by size:
1700 833 536
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2118 3069
Size: 2118 951
MscI TGG'CCA
Cuts at: 0 484 1084 1592 2039 3069
Size: 484 600 508 447 1030
Fragments arranged by size:
1030 600 508 484 447
MspA1I CmG'CkG
Cuts at: 0 138 1488 1682 2396 2919 3069
Size: 138 1350 194 714 523 150
Fragments arranged by size:
1350 714 523 194 150 138
NaeI GCC'GGC
Cuts at: 0 1264 3069
Size: 1264 1805
NarI GG'CG_CC
Cuts at: 0 537 2237 3069
Size: 537 1700 832
Fragments arranged by size:
1700 832 537
NcoI C'CATG_G
Cuts at: 0 1381 3023 3069
Size: 1381 1642 46
Fragments arranged by size:
1642 1381 46
NgoMIV G'CCGG_C
Cuts at: 0 1262 3069
Size: 1262 1807
NspI r_CATG'y
Cuts at: 0 852 882 2011 3069
Size: 852 30 1129 1058
Fragments arranged by size:
1129 1058 852 30
PasI CC'CwG_GG
Cuts at: 0 1942 3069
Size: 1942 1127
PflMI CCAn_nnn'nTGG
Cuts at: 0 1753 3069
Size: 1753 1316
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 113 145 3069
Size: 113 32 2924
Fragments arranged by size:
2924 113 32
PpuMI rG'GwC_Cy
Cuts at: 0 1615 1674 2129 3069
Size: 1615 59 455 940
Fragments arranged by size:
1615 940 455 59
PshAI GACnn'nnGTC
Cuts at: 0 323 3069
Size: 323 2746
PspOMI G'GGCC_C
Cuts at: 0 1210 1458 3069
Size: 1210 248 1611
Fragments arranged by size:
1611 1210 248
PstI C_TGCA'G
Cuts at: 0 185 470 683 698 949 1280 3069
Size: 185 285 213 15 251 331 1789
Fragments arranged by size:
1789 331 285 251 213 185 15
PvuII CAG'CTG
Cuts at: 0 138 1682 2919 3069
Size: 138 1544 1237 150
Fragments arranged by size:
1544 1237 150 138
SacI G_AGCT'C
Cuts at: 0 41 245 287 458 1414 1793 1991 2743
Size: 41 204 42 171 956 379 198 752
Cuts at: 2743 3069
Size: 326
Fragments arranged by size:
956 752 379 326 204 198 171 42
41
SanDI GG'GwC_CC
Cuts at: 0 1615 3069
Size: 1615 1454
SapI GCTCTTCn'nnn_
Cuts at: 0 29 250 1756 2577 3069
Size: 29 221 1506 821 492
Fragments arranged by size:
1506 821 492 221 29
SbfI CC_TGCA'GG
Cuts at: 0 683 3069
Size: 683 2386
SfcI C'TryA_G
Cuts at: 0 181 466 679 694 945 1276 3069
Size: 181 285 213 15 251 331 1793
Fragments arranged by size:
1793 331 285 251 213 181 15
SfoI GGC'GCC
Cuts at: 0 538 2238 3069
Size: 538 1700 831
Fragments arranged by size:
1700 831 538
SmaI CCC'GGG
Cuts at: 0 793 3069
Size: 793 2276
SmlI C'TyrA_G
Cuts at: 0 1043 1930 2155 2559 3069
Size: 1043 887 225 404 510
Fragments arranged by size:
1043 887 510 404 225
StuI AGG'CCT
Cuts at: 0 548 943 1147 1376 1543 3069
Size: 548 395 204 229 167 1526
Fragments arranged by size:
1526 548 395 229 204 167
StyI C'CwwG_G
Cuts at: 0 731 938 1381 1506 1538 2182 2246 3023
Size: 731 207 443 125 32 644 64 777
Cuts at: 3023 3069
Size: 46
Fragments arranged by size:
777 731 644 443 207 125 64 46
32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 529 607 1103 1212 1643 1833 1988 2259
Size: 529 78 496 109 431 190 155 271
Cuts at: 2259 3069
Size: 810
Fragments arranged by size:
810 529 496 431 271 190 155 109
78
TatI w'GTAC_w
Cuts at: 0 221 637 2711 3069
Size: 221 416 2074 358
Fragments arranged by size:
2074 416 358 221
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 169 201 692 724 1333 1365 3069
Size: 169 32 491 32 609 32 1704
Fragments arranged by size:
1704 609 491 169 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2368 3069
Size: 2368 701
XmaI C'CCGG_G
Cuts at: 0 791 3069
Size: 791 2278
Enzymes that do cut:
AarI AcuI AgeI AlfI AlwNI ApaI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BglI BglII BlpI
Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HincII KasI MmeI MscI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PflMI PpiI PpuMI PshAI
PspOMI PstI PvuII SacI SanDI SapI SbfI SfcI
SfoI SmaI SmlI StuI StyI TaqII TatI TstI
Tth111I XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BcgI BciVI BclI BfrBI BmrI BmtI BsaAI
BsaBI BsiEI BsiWI BsmI BspEI BspHI BsrDI BssSI
BstBI BstZ17I ClaI DraI DraIII EagI EcoRI EcoRV
FseI FspI FspAI HindIII HpaI KpnI MfeI MluI
MslI NdeI NheI NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PsiI PspXI PsrI PvuI RsrII
SacII SalI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI XbaI XcmI XhoI
XmnI ZraI