Restriction map for
pFN21AE2682
(Linear) (Six-base) MAPSORT of: KIEE2682.seq Check: 3823 from: 1 to: 1977
KIEE2682.orf
With 182 enzymes: SgfI *
March 10, 2017 10:38 ..
Acc65I G'GTAC_C
Cuts at: 0 1702 1977
Size: 1702 275
AclI AA'CG_TT
Cuts at: 0 1953 1977
Size: 1953 24
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 1977
Size: 97 1880
AflIII A'CryG_T
Cuts at: 0 1367 1977
Size: 1367 610
AlwNI CAG_nnn'CTG
Cuts at: 0 444 1977
Size: 444 1533
ApaI G_GGCC'C
Cuts at: 0 1354 1977
Size: 1354 623
ApoI r'AATT_y
Cuts at: 0 175 1332 1731 1977
Size: 175 1157 399 246
Fragments arranged by size:
1157 399 246 175
AvaI C'yCGr_G
Cuts at: 0 614 1617 1977
Size: 614 1003 360
Fragments arranged by size:
1003 614 360
AvrII C'CTAG_G
Cuts at: 0 702 1977
Size: 702 1275
BanI G'GyrC_C
Cuts at: 0 1159 1203 1702 1977
Size: 1159 44 499 275
Fragments arranged by size:
1159 499 275 44
BanII G_rGCy'C
Cuts at: 0 968 1187 1354 1977
Size: 968 219 167 623
Fragments arranged by size:
968 623 219 167
BbsI GAAGACnn'nnnn_
Cuts at: 0 11 346 1175 1297 1977
Size: 11 335 829 122 680
Fragments arranged by size:
829 680 335 122 11
BbvCI CC'TCA_GC
Cuts at: 0 1969 1977
Size: 1969 8
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 680 714 1977
Size: 680 34 1263
Fragments arranged by size:
1263 680 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 432 718 1977
Size: 432 286 1259
Fragments arranged by size:
1259 432 286
BglII A'GATC_T
Cuts at: 0 108 182 1782 1977
Size: 108 74 1600 195
Fragments arranged by size:
1600 195 108 74
Bme1580I G_kGCm'C
Cuts at: 0 299 1164 1354 1821 1977
Size: 299 865 190 467 156
Fragments arranged by size:
865 467 299 190 156
BmgBI CAC'GTC
Cuts at: 0 673 1977
Size: 673 1304
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 785 1201 1977
Size: 785 416 776
Fragments arranged by size:
785 776 416
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 437 791 1235 1977
Size: 386 51 354 444 742
Fragments arranged by size:
742 444 386 354 51
Bpu10I CC'TnA_GC
Cuts at: 0 1969 1977
Size: 1969 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 755 1855 1977
Size: 755 1100 122
Fragments arranged by size:
1100 755 122
BsaI GGTCTCn'nnnn_
Cuts at: 0 1010 1977
Size: 1010 967
BsaHI Gr'CG_yC
Cuts at: 0 290 1977
Size: 290 1687
BsaWI w'CCGG_w
Cuts at: 0 424 1324 1977
Size: 424 900 653
Fragments arranged by size:
900 653 424
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1051 1195 1977
Size: 1051 144 782
Fragments arranged by size:
1051 782 144
BseYI C'CCAG_C
Cuts at: 0 364 954 1002 1255 1977
Size: 364 590 48 253 722
Fragments arranged by size:
722 590 364 253 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 771 881 1977
Size: 771 110 1096
Fragments arranged by size:
1096 771 110
BsiEI CG_ry'CG
Cuts at: 0 690 1977
Size: 690 1287
BsiHKAI G_wGCw'C
Cuts at: 0 1187 1977
Size: 1187 790
BsmBI CGTCTCn'nnnn_
Cuts at: 0 663 1977
Size: 663 1314
Bsp1286I G_dGCh'C
Cuts at: 0 299 968 1164 1187 1354 1821 1977
Size: 299 669 196 23 167 467 156
Fragments arranged by size:
669 467 299 196 167 156 23
BspEI T'CCGG_A
Cuts at: 0 424 1324 1977
Size: 424 900 653
Fragments arranged by size:
900 653 424
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 386 586 1548 1977
Size: 386 200 962 429
Fragments arranged by size:
962 429 386 200
BsrDI GCAATG_nn'
Cuts at: 0 222 860 1977
Size: 222 638 1117
Fragments arranged by size:
1117 638 222
BssSI C'ACGA_G
Cuts at: 0 302 1977
Size: 302 1675
BstAPI GCAn_nnn'nTGC
Cuts at: 0 859 1977
Size: 859 1118
BstEII G'GTnAC_C
Cuts at: 0 1750 1977
Size: 1750 227
BstYI r'GATC_y
Cuts at: 0 15 108 182 1023 1782 1977
Size: 15 93 74 841 759 195
Fragments arranged by size:
841 759 195 93 74 15
BtgI C'CryG_G
Cuts at: 0 1190 1977
Size: 1190 787
DraIII CAC_nnn'GTG
Cuts at: 0 1435 1977
Size: 1435 542
EarI CTCTTCn'nnn_
Cuts at: 0 1840 1977
Size: 1840 137
EcoICRI GAG'CTC
Cuts at: 0 1185 1977
Size: 1185 792
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 386 437 791 1235 1977
Size: 97 289 51 354 444 742
Fragments arranged by size:
742 444 354 289 97 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1033 1825 1977
Size: 1033 792 152
Fragments arranged by size:
1033 792 152
EcoO109I rG'GnC_Cy
Cuts at: 0 618 1260 1350 1351 1977
Size: 618 642 90 1 626
Fragments arranged by size:
642 626 618 90 1
EcoRI G'AATT_C
Cuts at: 0 175 1977
Size: 175 1802
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 127 1977
Size: 127 1850
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 397 429 706 738 913 945 1015 1047
Size: 397 32 277 32 175 32 70 32
Cuts at: 1047 1524 1556 1977
Size: 477 32 421
Fragments arranged by size:
477 421 397 277 175 70 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 773 1805 1898 1977
Size: 773 1032 93 79
Fragments arranged by size:
1032 773 93 79
KpnI G_GTAC'C
Cuts at: 0 1706 1977
Size: 1706 271
MfeI C'AATT_G
Cuts at: 0 359 1742 1977
Size: 359 1383 235
Fragments arranged by size:
1383 359 235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 1122 1977
Size: 212 910 855
Fragments arranged by size:
910 855 212
MslI CAynn'nnrTG
Cuts at: 0 256 1977
Size: 256 1721
MspA1I CmG'CkG
Cuts at: 0 1255 1977
Size: 1255 722
NcoI C'CATG_G
Cuts at: 0 1190 1977
Size: 1190 787
NspI r_CATG'y
Cuts at: 0 261 358 1371 1977
Size: 261 97 1013 606
Fragments arranged by size:
1013 606 261 97
PciI A'CATG_T
Cuts at: 0 1367 1977
Size: 1367 610
PflMI CCAn_nnn'nTGG
Cuts at: 0 285 334 1977
Size: 285 49 1643
Fragments arranged by size:
1643 285 49
PfoI T'CCnGG_A
Cuts at: 0 941 1977
Size: 941 1036
PpuMI rG'GwC_Cy
Cuts at: 0 618 1260 1977
Size: 618 642 717
Fragments arranged by size:
717 642 618
PspOMI G'GGCC_C
Cuts at: 0 1350 1977
Size: 1350 627
PstI C_TGCA'G
Cuts at: 0 1665 1977
Size: 1665 312
PvuII CAG'CTG
Cuts at: 0 1255 1977
Size: 1255 722
SacI G_AGCT'C
Cuts at: 0 1187 1977
Size: 1187 790
SfcI C'TryA_G
Cuts at: 0 608 886 1661 1977
Size: 608 278 775 316
Fragments arranged by size:
775 608 316 278
SmaI CCC'GGG
Cuts at: 0 616 1619 1977
Size: 616 1003 358
Fragments arranged by size:
1003 616 358
SmlI C'TyrA_G
Cuts at: 0 770 1870 1977
Size: 770 1100 107
Fragments arranged by size:
1100 770 107
SrfI GCCC'GGGC
Cuts at: 0 1619 1977
Size: 1619 358
SspI AAT'ATT
Cuts at: 0 1199 1977
Size: 1199 778
StyI C'CwwG_G
Cuts at: 0 702 1190 1977
Size: 702 488 787
Fragments arranged by size:
787 702 488
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 704 1499 1977
Size: 704 795 478
Fragments arranged by size:
795 704 478
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 528 1197 1713 1977
Size: 528 669 516 264
Fragments arranged by size:
669 528 516 264
XmaI C'CCGG_G
Cuts at: 0 614 1617 1977
Size: 614 1003 360
Fragments arranged by size:
1003 614 360
XmnI GAAnn'nnTTC
Cuts at: 0 110 1072 1977
Size: 110 962 905
Fragments arranged by size:
962 905 110
Enzymes that do cut:
Acc65I AclI AcuI AflIII AlwNI ApaI ApoI AvaI
AvrII BanI BanII BbsI BbvCI BcgI BciVI BglII
Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspMI BsrDI BssSI BstAPI BstEII BstYI BtgI
DraIII EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
Hin4I HindIII KpnI MfeI MmeI MslI MspA1I NcoI
NspI PciI PflMI PfoI PpuMI PspOMI PstI PvuII
SacI SfcI SmaI SmlI SrfI SspI StyI TaqII
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI BaeI BamHI
BbeI BclI BfrBI BglI BlpI BmrI BmtI BsaAI
BsaBI BsaXI BsiWI BsmI BspHI BsrBI BsrFI BsrGI
BssHII BstBI BstXI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EciI EcoRV FseI
FspI FspAI HaeII HincII HpaI KasI MluI MscI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI StuI SwaI TatI TstI Tth111I XbaI XhoI
ZraI