Restriction map for
pFN21AE2670
(Linear) (Six-base) MAPSORT of: KIEE2670.seq Check: 6702 from: 1 to: 2133
KIEE2670.orf
With 182 enzymes: SgfI *
February 2, 2017 14:35 ..
AccI GT'mk_AC
Cuts at: 0 1179 1234 2133
Size: 1179 55 899
Fragments arranged by size:
1179 899 55
Acc65I G'GTAC_C
Cuts at: 0 799 2133
Size: 799 1334
AclI AA'CG_TT
Cuts at: 0 468 2133
Size: 468 1665
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 620 2047 2133
Size: 620 1427 86
Fragments arranged by size:
1427 620 86
AflIII A'CryG_T
Cuts at: 0 29 2133
Size: 29 2104
AlwNI CAG_nnn'CTG
Cuts at: 0 2044 2133
Size: 2044 89
ApaLI G'TGCA_C
Cuts at: 0 21 2133
Size: 21 2112
ApoI r'AATT_y
Cuts at: 0 523 2133
Size: 523 1610
AvaI C'yCGr_G
Cuts at: 0 1463 2133
Size: 1463 670
BamHI G'GATC_C
Cuts at: 0 1056 1885 1956 2133
Size: 1056 829 71 177
Fragments arranged by size:
1056 829 177 71
BanI G'GyrC_C
Cuts at: 0 381 591 799 2133
Size: 381 210 208 1334
Fragments arranged by size:
1334 381 210 208
BanII G_rGCy'C
Cuts at: 0 959 1026 1068 1448 1748 2015 2133
Size: 959 67 42 380 300 267 118
Fragments arranged by size:
959 380 300 267 118 67 42
BbsI GAAGACnn'nnnn_
Cuts at: 0 1996 2133
Size: 1996 137
BciVI GTATCCnnnnn_n'
Cuts at: 0 1423 2133
Size: 1423 710
BglII A'GATC_T
Cuts at: 0 2020 2133
Size: 2020 113
Bme1580I G_kGCm'C
Cuts at: 0 25 268 596 2133
Size: 25 243 328 1537
Fragments arranged by size:
1537 328 243 25
BmgBI CAC'GTC
Cuts at: 0 1515 2133
Size: 1515 618
BmrI ACTGGGnnnn_n'
Cuts at: 0 1442 1733 2133
Size: 1442 291 400
Fragments arranged by size:
1442 400 291
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 397 678 883 1890 1988 2001 2133
Size: 30 367 281 205 1007 98 13 132
Fragments arranged by size:
1007 367 281 205 132 98 30 13
Bpu10I CC'TnA_GC
Cuts at: 0 953 1961 2133
Size: 953 1008 172
Fragments arranged by size:
1008 953 172
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1100 1492 1663 2133
Size: 1100 392 171 470
Fragments arranged by size:
1100 470 392 171
BsaI GGTCTCn'nnnn_
Cuts at: 0 1671 2133
Size: 1671 462
BsaWI w'CCGG_w
Cuts at: 0 110 2133
Size: 110 2023
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 951 981 1055 1085 2133
Size: 951 30 74 30 1048
Fragments arranged by size:
1048 951 74 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 33 384 521 2133
Size: 33 351 137 1612
Fragments arranged by size:
1612 351 137 33
BseYI C'CCAG_C
Cuts at: 0 281 708 1096 1130 1354 2133
Size: 281 427 388 34 224 779
Fragments arranged by size:
779 427 388 281 224 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 1071 2133
Size: 793 278 1062
Fragments arranged by size:
1062 793 278
BsiHKAI G_wGCw'C
Cuts at: 0 25 959 1537 2133
Size: 25 934 578 596
Fragments arranged by size:
934 596 578 25
BsmI GAATG_Cn'
Cuts at: 0 106 846 2133
Size: 106 740 1287
Fragments arranged by size:
1287 740 106
Bsp1286I G_dGCh'C
Cuts at: 0 25 268 596 959 1026 1068 1448 1537
Size: 25 243 328 363 67 42 380 89
Cuts at: 1537 1748 2015 2133
Size: 211 267 118
Fragments arranged by size:
380 363 328 267 243 211 118 89
67 42 25
BspEI T'CCGG_A
Cuts at: 0 110 2133
Size: 110 2023
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1292 2133
Size: 1292 841
BstYI r'GATC_y
Cuts at: 0 1056 1885 1956 2020 2110 2133
Size: 1056 829 71 64 90 23
Fragments arranged by size:
1056 829 90 71 64 23
BstZ17I GTA'TAC
Cuts at: 0 1235 2133
Size: 1235 898
Bsu36I CC'TnA_GG
Cuts at: 0 397 1939 2133
Size: 397 1542 194
Fragments arranged by size:
1542 397 194
BtgI C'CryG_G
Cuts at: 0 1138 2133
Size: 1138 995
BtsI GCAGTG_nn'
Cuts at: 0 485 1694 2133
Size: 485 1209 439
Fragments arranged by size:
1209 485 439
EaeI y'GGCC_r
Cuts at: 0 287 725 2133
Size: 287 438 1408
Fragments arranged by size:
1408 438 287
EarI CTCTTCn'nnn_
Cuts at: 0 539 995 2133
Size: 539 456 1138
Fragments arranged by size:
1138 539 456
EcoICRI GAG'CTC
Cuts at: 0 957 2133
Size: 957 1176
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 397 620 678 883 1890 1988 2001
Size: 30 367 223 58 205 1007 98 13
Cuts at: 2001 2047 2133
Size: 46 86
Fragments arranged by size:
1007 367 223 205 98 86 58 46
30 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 788 2133
Size: 788 1345
EcoO109I rG'GnC_Cy
Cuts at: 0 1722 1876 2133
Size: 1722 154 257
Fragments arranged by size:
1722 257 154
EcoRV GAT'ATC
Cuts at: 0 174 2133
Size: 174 1959
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2077 2133
Size: 2077 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 121 153 2133
Size: 121 32 1980
Fragments arranged by size:
1980 121 32
HincII GTy'rAC
Cuts at: 0 552 2133
Size: 552 1581
KpnI G_GTAC'C
Cuts at: 0 803 2133
Size: 803 1330
MluI A'CGCG_T
Cuts at: 0 29 2133
Size: 29 2104
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 321 632 1807 1847 2133
Size: 44 277 311 1175 40 286
Fragments arranged by size:
1175 311 286 277 44 40
MspA1I CmG'CkG
Cuts at: 0 598 880 989 1684 1732 1892 2133
Size: 598 282 109 695 48 160 241
Fragments arranged by size:
695 598 282 241 160 109 48
NcoI C'CATG_G
Cuts at: 0 1138 2133
Size: 1138 995
PasI CC'CwG_GG
Cuts at: 0 1043 2133
Size: 1043 1090
PflMI CCAn_nnn'nTGG
Cuts at: 0 1156 2133
Size: 1156 977
PfoI T'CCnGG_A
Cuts at: 0 1059 2133
Size: 1059 1074
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 730 762 2133
Size: 730 32 1371
Fragments arranged by size:
1371 730 32
PpuMI rG'GwC_Cy
Cuts at: 0 1722 1876 2133
Size: 1722 154 257
Fragments arranged by size:
1722 257 154
PstI C_TGCA'G
Cuts at: 0 994 1755 2133
Size: 994 761 378
Fragments arranged by size:
994 761 378
PvuII CAG'CTG
Cuts at: 0 880 989 1732 1892 2133
Size: 880 109 743 160 241
Fragments arranged by size:
880 743 241 160 109
SacI G_AGCT'C
Cuts at: 0 959 2133
Size: 959 1174
SfcI C'TryA_G
Cuts at: 0 990 1727 1751 1976 2133
Size: 990 737 24 225 157
Fragments arranged by size:
990 737 225 157 24
SmlI C'TyrA_G
Cuts at: 0 1115 1463 1507 1642 2133
Size: 1115 348 44 135 491
Fragments arranged by size:
1115 491 348 135 44
StyI C'CwwG_G
Cuts at: 0 1138 2133
Size: 1138 995
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 238 472 628 2133
Size: 238 234 156 1505
Fragments arranged by size:
1505 238 234 156
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 951 983 2133
Size: 951 32 1150
Fragments arranged by size:
1150 951 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 232 2005 2133
Size: 232 1773 128
Fragments arranged by size:
1773 232 128
XhoI C'TCGA_G
Cuts at: 0 1463 2133
Size: 1463 670
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AvaI BamHI BanI BanII BbsI BciVI BglII Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI
BstYI BstZ17I Bsu36I BtgI BtsI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FalI Hin4I HincII KpnI
MluI MmeI MspA1I NcoI PasI PflMI PfoI PpiI
PpuMI PstI PvuII SacI SfcI SmlI StyI TaqII
TstI XcmI XhoI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BcgI BclI BfrBI BglI BlpI BmtI BplI
BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BtgZI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoRI FseI FspI FspAI HaeII HindIII HpaI
KasI MfeI MscI MslI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PmeI
PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI Tth111I XbaI XmaI XmnI
ZraI