Restriction map for
pFN21AE2667
(Linear) (Six-base) MAPSORT of: KIEE2667.seq Check: 2438 from: 1 to: 3315
KIEE2667.orf
With 182 enzymes: SgfI *
January 20, 2017 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2508 2826 3315
Size: 2508 318 489
Fragments arranged by size:
2508 489 318
AccI GT'mk_AC
Cuts at: 0 74 3315
Size: 74 3241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 895 1724 2089 3315
Size: 301 594 829 365 1226
Fragments arranged by size:
1226 829 594 365 301
AhdI GACnn_n'nnGTC
Cuts at: 0 1812 2159 3315
Size: 1812 347 1156
Fragments arranged by size:
1812 1156 347
AleI CACnn'nnGTG
Cuts at: 0 31 3315
Size: 31 3284
AlwNI CAG_nnn'CTG
Cuts at: 0 1621 2523 3255 3315
Size: 1621 902 732 60
Fragments arranged by size:
1621 902 732 60
ApaI G_GGCC'C
Cuts at: 0 1167 1407 1528 1719 2583 3315
Size: 1167 240 121 191 864 732
Fragments arranged by size:
1167 864 732 240 191 121
ApoI r'AATT_y
Cuts at: 0 1288 3315
Size: 1288 2027
AvaI C'yCGr_G
Cuts at: 0 373 677 2165 2776 3315
Size: 373 304 1488 611 539
Fragments arranged by size:
1488 611 539 373 304
BanI G'GyrC_C
Cuts at: 0 472 858 1479 2252 3285 3315
Size: 472 386 621 773 1033 30
Fragments arranged by size:
1033 773 621 472 386 30
BanII G_rGCy'C
Cuts at: 0 494 1132 1167 1253 1407 1528 1640 1719
Size: 494 638 35 86 154 121 112 79
Cuts at: 1719 2291 2299 2583 3315
Size: 572 8 284 732
Fragments arranged by size:
732 638 572 494 284 154 121 112
86 79 35 8
BbeI G_GCGC'C
Cuts at: 0 862 2256 3315
Size: 862 1394 1059
Fragments arranged by size:
1394 1059 862
BbsI GAAGACnn'nnnn_
Cuts at: 0 342 1730 3315
Size: 342 1388 1585
Fragments arranged by size:
1585 1388 342
BbvCI CC'TCA_GC
Cuts at: 0 1157 1974 3315
Size: 1157 817 1341
Fragments arranged by size:
1341 1157 817
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3004 3038 3315
Size: 3004 34 277
Fragments arranged by size:
3004 277 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1686 2341 2945 2995 3019 3315
Size: 1686 655 604 50 24 296
Fragments arranged by size:
1686 655 604 296 50 24
BlpI GC'TnA_GC
Cuts at: 0 553 1627 3315
Size: 553 1074 1688
Fragments arranged by size:
1688 1074 553
Bme1580I G_kGCm'C
Cuts at: 0 309 1167 1407 1484 1528 1719 2583 2654
Size: 309 858 240 77 44 191 864 71
Cuts at: 2654 3315
Size: 661
Fragments arranged by size:
864 858 661 309 240 191 77 71
44
BmgBI CAC'GTC
Cuts at: 0 39 465 3315
Size: 39 426 2850
Fragments arranged by size:
2850 426 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 919 2342 2847 3315
Size: 919 1423 505 468
Fragments arranged by size:
1423 919 505 468
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 679 731 1098 1118 2545 2590 2713
Size: 544 135 52 367 20 1427 45 123
Cuts at: 2713 2789 3238 3315
Size: 76 449 77
Fragments arranged by size:
1427 544 449 367 135 123 77 76
52 45 20
Bpu10I CC'TnA_GC
Cuts at: 0 1157 1444 1622 1974 3315
Size: 1157 287 178 352 1341
Fragments arranged by size:
1341 1157 352 287 178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1552 1912 2275 3315
Size: 1552 360 363 1040
Fragments arranged by size:
1552 1040 363 360
BsaI GGTCTCn'nnnn_
Cuts at: 0 1759 2256 3315
Size: 1759 497 1059
Fragments arranged by size:
1759 1059 497
BsaAI yAC'GTr
Cuts at: 0 72 513 1377 3315
Size: 72 441 864 1938
Fragments arranged by size:
1938 864 441 72
BsaBI GATnn'nnATC
Cuts at: 0 2236 3315
Size: 2236 1079
BsaHI Gr'CG_yC
Cuts at: 0 479 785 859 979 2253 2948 3315
Size: 479 306 74 120 1274 695 367
Fragments arranged by size:
1274 695 479 367 306 120 74
BsaWI w'CCGG_w
Cuts at: 0 122 353 803 3315
Size: 122 231 450 2512
Fragments arranged by size:
2512 450 231 122
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 91 121 1182 1212 1570 1600 1828 1858
Size: 91 30 1061 30 358 30 228 30
Cuts at: 1858 2527 2557 3187 3217 3315
Size: 669 30 630 30 98
Fragments arranged by size:
1061 669 630 358 228 98 91 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 643 733 1534 1591 1648 1777 1818 1848
Size: 643 90 801 57 57 129 41 30
Cuts at: 1848 1962 2010 2013 2022 2025 2028 2952 3190
Size: 114 48 3 9 3 3 924 238
Cuts at: 3190 3315
Size: 125
Fragments arranged by size:
924 801 643 238 129 125 114 90
57 57 48 41 30 9 3 3
3
BseYI C'CCAG_C
Cuts at: 0 440 638 854 1400 1498 1676 2146 2391
Size: 440 198 216 546 98 178 470 245
Cuts at: 2391 2576 3059 3281 3315
Size: 185 483 222 34
Fragments arranged by size:
546 483 470 440 245 222 216 198
185 178 98 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 757 1399 2536 3315
Size: 37 720 642 1137 779
Fragments arranged by size:
1137 779 720 642 37
BsiHKAI G_wGCw'C
Cuts at: 0 622 3315
Size: 622 2693
BsmI GAATG_Cn'
Cuts at: 0 1600 1947 3315
Size: 1600 347 1368
Fragments arranged by size:
1600 1368 347
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3153 3315
Size: 3153 162
Bsp1286I G_dGCh'C
Cuts at: 0 309 494 622 1132 1167 1253 1407 1484
Size: 309 185 128 510 35 86 154 77
Cuts at: 1484 1528 1640 1719 2291 2299 2583 2654 3315
Size: 44 112 79 572 8 284 71 661
Fragments arranged by size:
661 572 510 309 284 185 154 128
112 86 79 77 71 44 35 8
BspEI T'CCGG_A
Cuts at: 0 803 3315
Size: 803 2512
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 155 902 971 2508 2826 3315
Size: 155 747 69 1537 318 489
Fragments arranged by size:
1537 747 489 318 155 69
BsrBI CCG'CTC
Cuts at: 0 602 2078 2540 3315
Size: 602 1476 462 775
Fragments arranged by size:
1476 775 602 462
BsrDI GCAATG_nn'
Cuts at: 0 1031 1074 1280 3315
Size: 1031 43 206 2035
Fragments arranged by size:
2035 1031 206 43
BsrFI r'CCGG_y
Cuts at: 0 919 1685 1695 3315
Size: 919 766 10 1620
Fragments arranged by size:
1620 919 766 10
BstXI CCAn_nnnn'nTGG
Cuts at: 0 33 501 657 2281 3315
Size: 33 468 156 1624 1034
Fragments arranged by size:
1624 1034 468 156 33
BstYI r'GATC_y
Cuts at: 0 233 3251 3315
Size: 233 3018 64
Fragments arranged by size:
3018 233 64
BstZ17I GTA'TAC
Cuts at: 0 75 3315
Size: 75 3240
Bsu36I CC'TnA_GG
Cuts at: 0 1656 2796 3315
Size: 1656 1140 519
Fragments arranged by size:
1656 1140 519
BtgI C'CryG_G
Cuts at: 0 29 1088 1558 1640 2100 2280 2501 3206
Size: 29 1059 470 82 460 180 221 705
Cuts at: 3206 3315
Size: 109
Fragments arranged by size:
1059 705 470 460 221 180 109 82
29
BtsI GCAGTG_nn'
Cuts at: 0 1089 2972 3315
Size: 1089 1883 343
Fragments arranged by size:
1883 1089 343
ClaI AT'CG_AT
Cuts at: 0 290 1421 3315
Size: 290 1131 1894
Fragments arranged by size:
1894 1131 290
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2040 2075 3315
Size: 2040 35 1240
Fragments arranged by size:
2040 1240 35
DraIII CAC_nnn'GTG
Cuts at: 0 340 3315
Size: 340 2975
DrdI GACnn_nn'nnGTC
Cuts at: 0 1108 3315
Size: 1108 2207
EaeI y'GGCC_r
Cuts at: 0 186 917 1142 1792 2927 3011 3315
Size: 186 731 225 650 1135 84 304
Fragments arranged by size:
1135 731 650 304 225 186 84
EarI CTCTTCn'nnn_
Cuts at: 0 244 268 710 1707 1754 2031 2046 3315
Size: 244 24 442 997 47 277 15 1269
Fragments arranged by size:
1269 997 442 277 244 47 24 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 544 679 731 895 1098 1118 1724
Size: 301 243 135 52 164 203 20 606
Cuts at: 1724 2089 2545 2590 2713 2789 3238 3315
Size: 365 456 45 123 76 449 77
Fragments arranged by size:
606 456 449 365 301 243 203 164
135 123 77 76 52 45 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 877 1759 3315
Size: 877 882 1556
Fragments arranged by size:
1556 882 877
EcoO109I rG'GnC_Cy
Cuts at: 0 195 642 1026 1404 1524 1525 2580 2849
Size: 195 447 384 378 120 1 1055 269
Cuts at: 2849 3315
Size: 466
Fragments arranged by size:
1055 466 447 384 378 269 195 120
1
EcoRI G'AATT_C
Cuts at: 0 1288 3315
Size: 1288 2027
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 255 3315
Size: 255 3060
FseI GG_CCGG'CC
Cuts at: 0 923 3315
Size: 923 2392
HaeII r_GCGC'y
Cuts at: 0 862 2256 2465 3315
Size: 862 1394 209 850
Fragments arranged by size:
1394 862 850 209
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 91 123 1140 1172 1182 1214 1342 1374
Size: 91 32 1017 32 10 32 128 32
Cuts at: 1374 1639 1671 1798 1828 1830 1860 2145 2177
Size: 265 32 127 30 2 30 285 32
Cuts at: 2177 3187 3219 3315
Size: 1010 32 96
Fragments arranged by size:
1017 1010 285 265 128 127 96 91
32 32 32 32 32 32 32 30
30 10 2
HincII GTy'rAC
Cuts at: 0 384 2733 3315
Size: 384 2349 582
Fragments arranged by size:
2349 582 384
KasI G'GCGC_C
Cuts at: 0 858 2252 3315
Size: 858 1394 1063
Fragments arranged by size:
1394 1063 858
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 548 713 1254 2399 2976 3315
Size: 548 165 541 1145 577 339
Fragments arranged by size:
1145 577 548 541 339 165
MscI TGG'CCA
Cuts at: 0 188 1144 3315
Size: 188 956 2171
Fragments arranged by size:
2171 956 188
MslI CAynn'nnrTG
Cuts at: 0 31 3076 3315
Size: 31 3045 239
Fragments arranged by size:
3045 239 31
MspA1I CmG'CkG
Cuts at: 0 440 1138 1161 1560 1713 2102 2259 2503
Size: 440 698 23 399 153 389 157 244
Cuts at: 2503 2809 2986 3315
Size: 306 177 329
Fragments arranged by size:
698 440 399 389 329 306 244 177
157 153 23
NaeI GCC'GGC
Cuts at: 0 921 1697 3315
Size: 921 776 1618
Fragments arranged by size:
1618 921 776
NarI GG'CG_CC
Cuts at: 0 859 2253 3315
Size: 859 1394 1062
Fragments arranged by size:
1394 1062 859
NcoI C'CATG_G
Cuts at: 0 3206 3315
Size: 3206 109
NdeI CA'TA_TG
Cuts at: 0 1797 3315
Size: 1797 1518
NgoMIV G'CCGG_C
Cuts at: 0 919 1695 3315
Size: 919 776 1620
Fragments arranged by size:
1620 919 776
NspI r_CATG'y
Cuts at: 0 2459 3315
Size: 2459 856
PasI CC'CwG_GG
Cuts at: 0 1125 3315
Size: 1125 2190
PflMI CCAn_nnn'nTGG
Cuts at: 0 2595 3255 3315
Size: 2595 660 60
Fragments arranged by size:
2595 660 60
PfoI T'CCnGG_A
Cuts at: 0 257 500 2477 3191 3254 3315
Size: 257 243 1977 714 63 61
Fragments arranged by size:
1977 714 257 243 63 61
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1957 1989 3315
Size: 1957 32 1326
Fragments arranged by size:
1957 1326 32
PpuMI rG'GwC_Cy
Cuts at: 0 195 642 3315
Size: 195 447 2673
Fragments arranged by size:
2673 447 195
PspOMI G'GGCC_C
Cuts at: 0 1163 1403 1524 1715 2579 3315
Size: 1163 240 121 191 864 736
Fragments arranged by size:
1163 864 736 240 191 121
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 121 153 3315
Size: 121 32 3162
Fragments arranged by size:
3162 121 32
PstI C_TGCA'G
Cuts at: 0 152 1712 3315
Size: 152 1560 1603
Fragments arranged by size:
1603 1560 152
PvuII CAG'CTG
Cuts at: 0 440 1138 2259 3315
Size: 440 698 1121 1056
Fragments arranged by size:
1121 1056 698 440
RsrII CG'GwC_CG
Cuts at: 0 2181 3315
Size: 2181 1134
SacII CC_GC'GG
Cuts at: 0 1561 2103 2504 3315
Size: 1561 542 401 811
Fragments arranged by size:
1561 811 542 401
SanDI GG'GwC_CC
Cuts at: 0 642 3315
Size: 642 2673
ScaI AGT'ACT
Cuts at: 0 1273 3315
Size: 1273 2042
SexAI A'CCwGG_T
Cuts at: 0 91 3315
Size: 91 3224
SfcI C'TryA_G
Cuts at: 0 148 1708 3315
Size: 148 1560 1607
Fragments arranged by size:
1607 1560 148
SfoI GGC'GCC
Cuts at: 0 860 2254 3315
Size: 860 1394 1061
Fragments arranged by size:
1394 1061 860
SmaI CCC'GGG
Cuts at: 0 2167 2778 3315
Size: 2167 611 537
Fragments arranged by size:
2167 611 537
SmlI C'TyrA_G
Cuts at: 0 1567 1927 2290 3301 3315
Size: 1567 360 363 1011 14
Fragments arranged by size:
1567 1011 363 360 14
SnaBI TAC'GTA
Cuts at: 0 72 3315
Size: 72 3243
StyI C'CwwG_G
Cuts at: 0 884 1475 1508 2111 2657 2879 2999 3206
Size: 884 591 33 603 546 222 120 207
Cuts at: 3206 3315
Size: 109
Fragments arranged by size:
884 603 591 546 222 207 120 109
33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1720 2169 2892 3273 3315
Size: 1720 449 723 381 42
Fragments arranged by size:
1720 723 449 381 42
TatI w'GTAC_w
Cuts at: 0 1271 3315
Size: 1271 2044
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 808 840 2527 2559 3315
Size: 808 32 1687 32 756
Fragments arranged by size:
1687 808 756 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 93 346 3315
Size: 93 253 2969
Fragments arranged by size:
2969 253 93
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3006 3213 3256 3315
Size: 3006 207 43 59
Fragments arranged by size:
3006 207 59 43
XmaI C'CCGG_G
Cuts at: 0 2165 2776 3315
Size: 2165 611 539
Fragments arranged by size:
2165 611 539
XmnI GAAnn'nnTTC
Cuts at: 0 3271 3315
Size: 3271 44
Enzymes that do cut:
AarI AccI AcuI AhdI AleI AlwNI ApaI ApoI
AvaI BanI BanII BbeI BbsI BbvCI BcgI BglI
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BstXI BstYI BstZ17I Bsu36I BtgI BtsI ClaI
CspCI DraIII DrdI EaeI EarI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI HaeII Hin4I HincII KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PasI PflMI PfoI PpiI PpuMI PspOMI PsrI
PstI PvuII RsrII SacII SanDI ScaI SexAI SfcI
SfoI SmaI SmlI SnaBI StyI TaqII TatI TstI
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BciVI BclI BfrBI BglII BmtI BplI BsiEI BsiWI
BspHI BsrGI BssHII BssSI BstAPI BstBI BstEII BtgZI
DraI EagI EciI EcoICRI EcoRV FspI FspAI HindIII
HpaI KpnI MfeI MluI NheI NotI NruI NsiI
PacI PciI PmeI PmlI PshAI PsiI PspXI PvuI
SacI SalI SapI SbfI SfiI SgfI SgrAI SpeI
SphI SrfI SspI StuI SwaI XbaI XhoI ZraI