Restriction map for
pFN21AE2651
(Linear) (Six-base) MAPSORT of: KIEE2651.seq Check: 6456 from: 1 to: 2481
KIEE2651.orf
With 182 enzymes: SgfI *
November 16, 2016 14:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1191 2481
Size: 1191 1290
AatII G_ACGT'C
Cuts at: 0 407 2481
Size: 407 2074
AccI GT'mk_AC
Cuts at: 0 1493 2481
Size: 1493 988
Acc65I G'GTAC_C
Cuts at: 0 1230 2481
Size: 1230 1251
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 727 898 2203 2481
Size: 286 441 171 1305 278
Fragments arranged by size:
1305 441 286 278 171
AflIII A'CryG_T
Cuts at: 0 887 2252 2481
Size: 887 1365 229
Fragments arranged by size:
1365 887 229
AleI CACnn'nnGTG
Cuts at: 0 730 985 2481
Size: 730 255 1496
Fragments arranged by size:
1496 730 255
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1930 1962 2481
Size: 1930 32 519
Fragments arranged by size:
1930 519 32
AlwNI CAG_nnn'CTG
Cuts at: 0 414 834 2481
Size: 414 420 1647
Fragments arranged by size:
1647 420 414
ApaI G_GGCC'C
Cuts at: 0 1538 2481
Size: 1538 943
AvaI C'yCGr_G
Cuts at: 0 48 2341 2481
Size: 48 2293 140
Fragments arranged by size:
2293 140 48
AvrII C'CTAG_G
Cuts at: 0 1071 2481
Size: 1071 1410
BamHI G'GATC_C
Cuts at: 0 2142 2481
Size: 2142 339
BanI G'GyrC_C
Cuts at: 0 87 1230 1504 1570 2481
Size: 87 1143 274 66 911
Fragments arranged by size:
1143 911 274 87 66
BanII G_rGCy'C
Cuts at: 0 1096 1538 2111 2339 2481
Size: 1096 442 573 228 142
Fragments arranged by size:
1096 573 442 228 142
BbeI G_GCGC'C
Cuts at: 0 1574 2481
Size: 1574 907
BbsI GAAGACnn'nnnn_
Cuts at: 0 1965 2481
Size: 1965 516
BbvCI CC'TCA_GC
Cuts at: 0 2228 2481
Size: 2228 253
BfrBI ATG'CAT
Cuts at: 0 1140 2481
Size: 1140 1341
BglII A'GATC_T
Cuts at: 0 881 2481
Size: 881 1600
BlpI GC'TnA_GC
Cuts at: 0 10 2481
Size: 10 2471
Bme1580I G_kGCm'C
Cuts at: 0 722 1538 2481
Size: 722 816 943
Fragments arranged by size:
943 816 722
BmgBI CAC'GTC
Cuts at: 0 2253 2481
Size: 2253 228
BmrI ACTGGGnnnn_n'
Cuts at: 0 1644 1892 2481
Size: 1644 248 589
Fragments arranged by size:
1644 589 248
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 411 2481
Size: 411 2070
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 2232 2326 2399 2481
Size: 470 1762 94 73 82
Fragments arranged by size:
1762 470 94 82 73
Bpu10I CC'TnA_GC
Cuts at: 0 2228 2481
Size: 2228 253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 730 1627 2029 2443 2481
Size: 730 897 402 414 38
Fragments arranged by size:
897 730 414 402 38
BsaI GGTCTCn'nnnn_
Cuts at: 0 382 587 1522 2068 2481
Size: 382 205 935 546 413
Fragments arranged by size:
935 546 413 382 205
BsaAI yAC'GTr
Cuts at: 0 384 684 888 2481
Size: 384 300 204 1593
Fragments arranged by size:
1593 384 300 204
BsaHI Gr'CG_yC
Cuts at: 0 404 1571 2481
Size: 404 1167 910
Fragments arranged by size:
1167 910 404
BsaWI w'CCGG_w
Cuts at: 0 350 537 1058 2481
Size: 350 187 521 1423
Fragments arranged by size:
1423 521 350 187
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1714 1744 2481
Size: 1714 30 737
Fragments arranged by size:
1714 737 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2017 2020 2347 2481
Size: 2017 3 327 134
Fragments arranged by size:
2017 327 134 3
BseYI C'CCAG_C
Cuts at: 0 687 1163 1599 2056 2356 2481
Size: 687 476 436 457 300 125
Fragments arranged by size:
687 476 457 436 300 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 737 1015 1219 2015 2481
Size: 301 436 278 204 796 466
Fragments arranged by size:
796 466 436 301 278 204
BsiEI CG_ry'CG
Cuts at: 0 716 1019 2481
Size: 716 303 1462
Fragments arranged by size:
1462 716 303
BsiHKAI G_wGCw'C
Cuts at: 0 2339 2481
Size: 2339 142
Bsp1286I G_dGCh'C
Cuts at: 0 722 1096 1538 2111 2339 2481
Size: 722 374 442 573 228 142
Fragments arranged by size:
722 573 442 374 228 142
BspEI T'CCGG_A
Cuts at: 0 350 2481
Size: 350 2131
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 842 1191 1289 1331 2481
Size: 842 349 98 42 1150
Fragments arranged by size:
1150 842 349 98 42
BsrBI CCG'CTC
Cuts at: 0 603 1698 2481
Size: 603 1095 783
Fragments arranged by size:
1095 783 603
BsrFI r'CCGG_y
Cuts at: 0 738 2481
Size: 738 1743
BssSI C'ACGA_G
Cuts at: 0 844 2104 2481
Size: 844 1260 377
Fragments arranged by size:
1260 844 377
BstAPI GCAn_nnn'nTGC
Cuts at: 0 87 1323 2029 2481
Size: 87 1236 706 452
Fragments arranged by size:
1236 706 452 87
BstXI CCAn_nnnn'nTGG
Cuts at: 0 84 252 532 1264 1567 2088 2481
Size: 84 168 280 732 303 521 393
Fragments arranged by size:
732 521 393 303 280 168 84
BstYI r'GATC_y
Cuts at: 0 587 881 1427 1904 2142 2481
Size: 587 294 546 477 238 339
Fragments arranged by size:
587 546 477 339 294 238
Bsu36I CC'TnA_GG
Cuts at: 0 1065 1151 2481
Size: 1065 86 1330
Fragments arranged by size:
1330 1065 86
BtgI C'CryG_G
Cuts at: 0 1097 2481
Size: 1097 1384
BtsI GCAGTG_nn'
Cuts at: 0 2027 2222 2481
Size: 2027 195 259
Fragments arranged by size:
2027 259 195
DraIII CAC_nnn'GTG
Cuts at: 0 1089 2481
Size: 1089 1392
EaeI y'GGCC_r
Cuts at: 0 198 578 713 817 1016 1100 1706 1895
Size: 198 380 135 104 199 84 606 189
Cuts at: 1895 2481
Size: 586
Fragments arranged by size:
606 586 380 199 198 189 135 104
84
EagI C'GGCC_G
Cuts at: 0 713 1016 2481
Size: 713 303 1465
Fragments arranged by size:
1465 713 303
EarI CTCTTCn'nnn_
Cuts at: 0 466 1039 1928 2481
Size: 466 573 889 553
Fragments arranged by size:
889 573 553 466
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2203 2481
Size: 2203 278
EcoICRI GAG'CTC
Cuts at: 0 2337 2481
Size: 2337 144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 470 727 898 2203 2232 2326 2399
Size: 286 184 257 171 1305 29 94 73
Cuts at: 2399 2481
Size: 82
Fragments arranged by size:
1305 286 257 184 171 94 82 73
29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 865 2481
Size: 865 1616
EcoO109I rG'GnC_Cy
Cuts at: 0 831 873 1434 1535 1596 1803 2178 2481
Size: 831 42 561 101 61 207 375 303
Fragments arranged by size:
831 561 375 303 207 101 61 42
HaeII r_GCGC'y
Cuts at: 0 17 1574 1890 2481
Size: 17 1557 316 591
Fragments arranged by size:
1557 591 316 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1117 1149 1714 1746 2481
Size: 1117 32 565 32 735
Fragments arranged by size:
1117 735 565 32 32
HindIII A'AGCT_T
Cuts at: 0 114 514 2481
Size: 114 400 1967
Fragments arranged by size:
1967 400 114
KasI G'GCGC_C
Cuts at: 0 1570 2481
Size: 1570 911
KpnI G_GTAC'C
Cuts at: 0 1234 2481
Size: 1234 1247
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1079 1898 2481
Size: 1079 819 583
Fragments arranged by size:
1079 819 583
MscI TGG'CCA
Cuts at: 0 200 580 1102 1708 1897 2481
Size: 200 380 522 606 189 584
Fragments arranged by size:
606 584 522 380 200 189
MslI CAynn'nnrTG
Cuts at: 0 82 250 550 730 985 1135 1262 2086
Size: 82 168 300 180 255 150 127 824
Cuts at: 2086 2089 2481
Size: 3 392
Fragments arranged by size:
824 392 300 255 180 168 150 127
82 3
MspA1I CmG'CkG
Cuts at: 0 232 2060 2419 2481
Size: 232 1828 359 62
Fragments arranged by size:
1828 359 232 62
NaeI GCC'GGC
Cuts at: 0 740 2481
Size: 740 1741
NarI GG'CG_CC
Cuts at: 0 1571 2481
Size: 1571 910
NgoMIV G'CCGG_C
Cuts at: 0 738 2481
Size: 738 1743
NsiI A_TGCA'T
Cuts at: 0 1142 2481
Size: 1142 1339
NspI r_CATG'y
Cuts at: 0 424 1144 1996 2481
Size: 424 720 852 485
Fragments arranged by size:
852 720 485 424
PasI CC'CwG_GG
Cuts at: 0 1791 2481
Size: 1791 690
PflMI CCAn_nnn'nTGG
Cuts at: 0 687 1089 1781 2481
Size: 687 402 692 700
Fragments arranged by size:
700 692 687 402
PfoI T'CCnGG_A
Cuts at: 0 466 500 860 1394 1732 2481
Size: 466 34 360 534 338 749
Fragments arranged by size:
749 534 466 360 338 34
PmlI CAC'GTG
Cuts at: 0 384 684 2481
Size: 384 300 1797
Fragments arranged by size:
1797 384 300
PpuMI rG'GwC_Cy
Cuts at: 0 831 1434 1596 2178 2481
Size: 831 603 162 582 303
Fragments arranged by size:
831 603 582 303 162
PspOMI G'GGCC_C
Cuts at: 0 1534 2481
Size: 1534 947
PstI C_TGCA'G
Cuts at: 0 59 2481
Size: 59 2422
PvuII CAG'CTG
Cuts at: 0 232 2419 2481
Size: 232 2187 62
Fragments arranged by size:
2187 232 62
SacI G_AGCT'C
Cuts at: 0 2339 2481
Size: 2339 142
SanDI GG'GwC_CC
Cuts at: 0 1434 2481
Size: 1434 1047
SapI GCTCTTCn'nnn_
Cuts at: 0 1039 2481
Size: 1039 1442
SexAI A'CCwGG_T
Cuts at: 0 1841 1967 2481
Size: 1841 126 514
Fragments arranged by size:
1841 514 126
SfcI C'TryA_G
Cuts at: 0 55 1828 2327 2481
Size: 55 1773 499 154
Fragments arranged by size:
1773 499 154 55
SfoI GGC'GCC
Cuts at: 0 1572 2481
Size: 1572 909
SmaI CCC'GGG
Cuts at: 0 50 2481
Size: 50 2431
SmlI C'TyrA_G
Cuts at: 0 745 1642 2044 2458 2481
Size: 745 897 402 414 23
Fragments arranged by size:
897 745 414 402 23
StuI AGG'CCT
Cuts at: 0 316 341 1070 1588 2401 2481
Size: 316 25 729 518 813 80
Fragments arranged by size:
813 729 518 316 80 25
StyI C'CwwG_G
Cuts at: 0 507 581 1071 1583 2481
Size: 507 74 490 512 898
Fragments arranged by size:
898 512 507 490 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1783 2481
Size: 1783 698
TatI w'GTAC_w
Cuts at: 0 1406 2481
Size: 1406 1075
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1231 1263 1472 1504 1535 1567 2481
Size: 1231 32 209 32 31 32 914
Fragments arranged by size:
1231 914 209 32 32 32 31
Tth111I GACn'n_nGTC
Cuts at: 0 1050 1650 1969 2481
Size: 1050 600 319 512
Fragments arranged by size:
1050 600 512 319
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 193 1408 1778 1782 2301 2481
Size: 193 1215 370 4 519 180
Fragments arranged by size:
1215 519 370 193 180 4
XmaI C'CCGG_G
Cuts at: 0 48 2481
Size: 48 2433
XmnI GAAnn'nnTTC
Cuts at: 0 475 2481
Size: 475 2006
ZraI GAC'GTC
Cuts at: 0 405 2481
Size: 405 2076
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AleI AloI
AlwNI ApaI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspMI BsrBI BsrFI BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtsI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII KasI KpnI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI
NspI PasI PflMI PfoI PmlI PpuMI PspOMI PstI
PvuII SacI SanDI SapI SexAI SfcI SfoI SmaI
SmlI StuI StyI TaqII TatI TstI Tth111I XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflII AgeI AhdI AlfI ApaLI ApoI
AscI AseI AsiSI BaeI BcgI BciVI BclI BglI
BmtI BsaBI BsiWI BsmI BsmBI BspHI BsrDI BsrGI
BssHII BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DrdI EcoRI EcoRV FalI FseI FspI FspAI HincII
HpaI MfeI MluI NcoI NdeI NheI NotI NruI
PacI PciI PmeI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SbfI ScaI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI
XhoI