Restriction map for
pFN21AE2634
(Linear) (Six-base) MAPSORT of: KIEE2634.seq Check: 1786 from: 1 to: 4434
KIEE2634.orf
With 182 enzymes: SgfI *
August 9, 2016 17:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1635 1690 4434
Size: 1635 55 2744
Fragments arranged by size:
2744 1635 55
AclI AA'CG_TT
Cuts at: 0 863 4434
Size: 863 3571
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1288 2509 2914 3166 4001 4042 4185 4434
Size: 1288 1221 405 252 835 41 143 249
Fragments arranged by size:
1288 1221 835 405 252 249 143 41
AfeI AGC'GCT
Cuts at: 0 3661 4434
Size: 3661 773
AflII C'TTAA_G
Cuts at: 0 3052 4434
Size: 3052 1382
AflIII A'CryG_T
Cuts at: 0 1581 4434
Size: 1581 2853
AhdI GACnn_n'nnGTC
Cuts at: 0 492 4434
Size: 492 3942
AleI CACnn'nnGTG
Cuts at: 0 385 2018 4434
Size: 385 1633 2416
Fragments arranged by size:
2416 1633 385
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 139 640 3549 4348 4434
Size: 139 501 2909 799 86
Fragments arranged by size:
2909 799 501 139 86
AlwNI CAG_nnn'CTG
Cuts at: 0 398 765 786 963 1551 1902 2133 2163
Size: 398 367 21 177 588 351 231 30
Cuts at: 2163 2487 2532 4434
Size: 324 45 1902
Fragments arranged by size:
1902 588 398 367 351 324 231 177
45 30 21
ApaI G_GGCC'C
Cuts at: 0 2616 2967 3779 4434
Size: 2616 351 812 655
Fragments arranged by size:
2616 812 655 351
ApaLI G'TGCA_C
Cuts at: 0 337 4434
Size: 337 4097
ApoI r'AATT_y
Cuts at: 0 79 130 3475 4434
Size: 79 51 3345 959
Fragments arranged by size:
3345 959 79 51
AvaI C'yCGr_G
Cuts at: 0 1322 1657 2197 3409 4434
Size: 1322 335 540 1212 1025
Fragments arranged by size:
1322 1212 1025 540 335
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 133 166 1796 1829 3862 3895 4434
Size: 133 33 1630 33 2033 33 539
Fragments arranged by size:
2033 1630 539 133 33 33 33
BamHI G'GATC_C
Cuts at: 0 260 4434
Size: 260 4174
BanI G'GyrC_C
Cuts at: 0 3575 4434
Size: 3575 859
BanII G_rGCy'C
Cuts at: 0 290 442 893 1444 1841 1975 2196 2520
Size: 290 152 451 551 397 134 221 324
Cuts at: 2520 2616 2798 2967 2999 3746 3779 4434
Size: 96 182 169 32 747 33 655
Fragments arranged by size:
747 655 551 451 397 324 290 221
182 169 152 134 96 33 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 3263 3989 4164 4203 4434
Size: 3263 726 175 39 231
Fragments arranged by size:
3263 726 231 175 39
BbvCI CC'TCA_GC
Cuts at: 0 787 1636 2447 3125 3223 3497 4434
Size: 787 849 811 678 98 274 937
Fragments arranged by size:
937 849 811 787 678 274 98
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1648 1682 3913 3947 4434
Size: 1648 34 2231 34 487
Fragments arranged by size:
2231 1648 487 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3939 4434
Size: 3939 495
BclI T'GATC_A
Cuts at: 0 165 4040 4434
Size: 165 3875 394
Fragments arranged by size:
3875 394 165
BglI GCCn_nnn'nGGC
Cuts at: 0 232 959 1927 1999 2065 2083 2584 2812
Size: 232 727 968 72 66 18 501 228
Cuts at: 2812 3086 3996 4434
Size: 274 910 438
Fragments arranged by size:
968 910 727 501 438 274 232 228
72 66 18
BlpI GC'TnA_GC
Cuts at: 0 436 887 1124 2437 2675 2717 3178 4434
Size: 436 451 237 1313 238 42 461 1256
Fragments arranged by size:
1313 1256 461 451 436 238 237 42
Bme1580I G_kGCm'C
Cuts at: 0 341 1278 2562 2616 2967 3779 4434
Size: 341 937 1284 54 351 812 655
Fragments arranged by size:
1284 937 812 655 351 341 54
BmrI ACTGGGnnnn_n'
Cuts at: 0 219 1172 4434
Size: 219 953 3262
Fragments arranged by size:
3262 953 219
BmtI G_CTAG'C
Cuts at: 0 1117 1462 2314 4434
Size: 1117 345 852 2120
Fragments arranged by size:
2120 1117 852 345
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2835 3900 4434
Size: 2835 1065 534
Fragments arranged by size:
2835 1065 534
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 13 579 877 985 1039 1348 1354 1531
Size: 13 566 298 108 54 309 6 177
Cuts at: 1531 1573 1615 1719 1882 1924 2008 2368 2686
Size: 42 42 104 163 42 84 360 318
Cuts at: 2686 2782 2860 4049 4434
Size: 96 78 1189 385
Fragments arranged by size:
1189 566 385 360 318 309 298 177
163 108 104 96 84 78 54 42
42 42 13 6
Bpu10I CC'TnA_GC
Cuts at: 0 787 1636 2188 2369 2447 3125 3223 3433
Size: 787 849 552 181 78 678 98 210
Cuts at: 3433 3497 3634 3723 4434
Size: 64 137 89 711
Fragments arranged by size:
849 787 711 678 552 210 181 137
98 89 78 64
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 617 1072 2805 2992 3115 4291 4341
Size: 166 451 455 1733 187 123 1176 50
Cuts at: 4341 4434
Size: 93
Fragments arranged by size:
1733 1176 455 451 187 166 123 93
50
BsaI GGTCTCn'nnnn_
Cuts at: 0 1532 1549 4434
Size: 1532 17 2885
Fragments arranged by size:
2885 1532 17
BsaBI GATnn'nnATC
Cuts at: 0 4132 4434
Size: 4132 302
BsaHI Gr'CG_yC
Cuts at: 0 493 2804 4434
Size: 493 2311 1630
Fragments arranged by size:
2311 1630 493
BsaWI w'CCGG_w
Cuts at: 0 3822 4434
Size: 3822 612
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1360 1390 1426 1456 1510 1540 1780 1810
Size: 1360 30 36 30 54 30 240 30
Cuts at: 1810 2107 2137 2614 2644 4434
Size: 297 30 477 30 1790
Fragments arranged by size:
1790 1360 477 297 240 54 36 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1003 1267 1384 1465 1468 1510 1612 1780
Size: 1003 264 117 81 3 42 102 168
Cuts at: 1780 1783 2155 2584 2614 2617 2638 2782 3112
Size: 3 372 429 30 3 21 144 330
Cuts at: 3112 3427 3514 3876 4167 4434
Size: 315 87 362 291 267
Fragments arranged by size:
1003 429 372 362 330 315 291 267
264 168 144 117 102 87 81 42
30 21 3 3 3
BseYI C'CCAG_C
Cuts at: 0 1559 1669 2222 2306 2588 2721 2854 2939
Size: 1559 110 553 84 282 133 133 85
Cuts at: 2939 3745 4166 4434
Size: 806 421 268
Fragments arranged by size:
1559 806 553 421 282 268 133 133
110 85 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2446 2500 2677 2969 3168 3737 4434
Size: 2446 54 177 292 199 569 697
Fragments arranged by size:
2446 697 569 292 199 177 54
BsiHKAI G_wGCw'C
Cuts at: 0 290 341 442 893 1444 1841 2468 2672
Size: 290 51 101 451 551 397 627 204
Cuts at: 2672 2798 2999 4434
Size: 126 201 1435
Fragments arranged by size:
1435 627 551 451 397 290 204 201
126 101 51
BsmI GAATG_Cn'
Cuts at: 0 3063 4434
Size: 3063 1371
Bsp1286I G_dGCh'C
Cuts at: 0 290 341 442 893 1278 1444 1841 1975
Size: 290 51 101 451 385 166 397 134
Cuts at: 1975 2196 2468 2520 2562 2616 2672 2798 2967
Size: 221 272 52 42 54 56 126 169
Cuts at: 2967 2999 3746 3779 4434
Size: 32 747 33 655
Fragments arranged by size:
747 655 451 397 385 290 272 221
169 166 134 126 101 56 54 52
51 42 33 32
BspHI T'CATG_A
Cuts at: 0 374 4434
Size: 374 4060
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 632 1635 1690 4434
Size: 632 1003 55 2744
Fragments arranged by size:
2744 1003 632 55
BsrBI CCG'CTC
Cuts at: 0 1662 3417 4434
Size: 1662 1755 1017
Fragments arranged by size:
1755 1662 1017
BsrDI GCAATG_nn'
Cuts at: 0 2545 4434
Size: 2545 1889
BsrFI r'CCGG_y
Cuts at: 0 1160 2914 3107 4434
Size: 1160 1754 193 1327
Fragments arranged by size:
1754 1327 1160 193
BssSI C'ACGA_G
Cuts at: 0 1437 4434
Size: 1437 2997
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1425 1923 4434
Size: 1425 498 2511
Fragments arranged by size:
2511 1425 498
BstEII G'GTnAC_C
Cuts at: 0 909 2020 4434
Size: 909 1111 2414
Fragments arranged by size:
2414 1111 909
BstXI CCAn_nnnn'nTGG
Cuts at: 0 189 2854 4140 4434
Size: 189 2665 1286 294
Fragments arranged by size:
2665 1286 294 189
BstYI r'GATC_y
Cuts at: 0 260 4434
Size: 260 4174
Bsu36I CC'TnA_GG
Cuts at: 0 2458 3082 3971 4434
Size: 2458 624 889 463
Fragments arranged by size:
2458 889 624 463
BtgI C'CryG_G
Cuts at: 0 1118 2747 3992 4434
Size: 1118 1629 1245 442
Fragments arranged by size:
1629 1245 1118 442
BtsI GCAGTG_nn'
Cuts at: 0 373 845 907 2158 2560 2920 3531 3579
Size: 373 472 62 1251 402 360 611 48
Cuts at: 3579 3723 4434
Size: 144 711
Fragments arranged by size:
1251 711 611 472 402 373 360 144
62 48
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3627 3662 4434
Size: 3627 35 772
Fragments arranged by size:
3627 772 35
EaeI y'GGCC_r
Cuts at: 0 233 523 2900 3215 3594 4142 4434
Size: 233 290 2377 315 379 548 292
Fragments arranged by size:
2377 548 379 315 292 290 233
EarI CTCTTCn'nnn_
Cuts at: 0 306 564 1829 3062 4434
Size: 306 258 1265 1233 1372
Fragments arranged by size:
1372 1265 1233 306 258
EcoICRI GAG'CTC
Cuts at: 0 288 440 891 1442 1839 2796 2997 4434
Size: 288 152 451 551 397 957 201 1437
Fragments arranged by size:
1437 957 551 451 397 288 201 152
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 13 579 877 985 1039 1288 1348 1354
Size: 13 566 298 108 54 249 60 6
Cuts at: 1354 1531 1573 1615 1719 1882 1924 2008 2368
Size: 177 42 42 104 163 42 84 360
Cuts at: 2368 2509 2686 2782 2860 2914 3166 4001 4042
Size: 141 177 96 78 54 252 835 41
Cuts at: 4042 4049 4185 4434
Size: 7 136 249
Fragments arranged by size:
835 566 360 298 252 249 249 177
177 163 141 136 108 104 96 84
78 60 54 54 42 42 42 41
13 7 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 235 1345 1786 2320 2620 2977 4356 4434
Size: 235 1110 441 534 300 357 1379 78
Fragments arranged by size:
1379 1110 534 441 357 300 235 78
EcoO109I rG'GnC_Cy
Cuts at: 0 960 1716 1779 2235 2613 2835 3269 3697
Size: 960 756 63 456 378 222 434 428
Cuts at: 3697 3776 4251 4434
Size: 79 475 183
Fragments arranged by size:
960 756 475 456 434 428 378 222
183 79 63
EcoRI G'AATT_C
Cuts at: 0 3475 4434
Size: 3475 959
EcoRV GAT'ATC
Cuts at: 0 3843 4434
Size: 3843 591
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2895 4434
Size: 2895 1539
FspI TGC'GCA
Cuts at: 0 2739 4434
Size: 2739 1695
HaeII r_GCGC'y
Cuts at: 0 1341 2847 3663 4434
Size: 1341 1506 816 771
Fragments arranged by size:
1506 1341 816 771
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 478 510 628 660 1129 1161 1360 1392
Size: 478 32 118 32 469 32 199 32
Cuts at: 1392 1510 1542 2507 2539 2614 2646 3937 3969
Size: 118 32 965 32 75 32 1291 32
Cuts at: 3969 4383 4415 4434
Size: 414 32 19
Fragments arranged by size:
1291 965 478 469 414 199 118 118
75 32 32 32 32 32 32 32
32 32 19
HincII GTy'rAC
Cuts at: 0 1260 4434
Size: 1260 3174
HindIII A'AGCT_T
Cuts at: 0 1216 4434
Size: 1216 3218
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 671 704 2379 4434
Size: 671 33 1675 2055
Fragments arranged by size:
2055 1675 671 33
MscI TGG'CCA
Cuts at: 0 235 525 4434
Size: 235 290 3909
Fragments arranged by size:
3909 290 235
MslI CAynn'nnrTG
Cuts at: 0 385 1586 2018 3991 4138 4330 4434
Size: 385 1201 432 1973 147 192 104
Fragments arranged by size:
1973 1201 432 385 192 147 104
MspA1I CmG'CkG
Cuts at: 0 765 796 853 1290 1308 1542 1563 1953
Size: 765 31 57 437 18 234 21 390
Cuts at: 1953 2133 2226 2331 2487 2664 2691 2716 2961
Size: 180 93 105 156 177 27 25 245
Cuts at: 2961 3018 3114 3560 4166 4236 4434
Size: 57 96 446 606 70 198
Fragments arranged by size:
765 606 446 437 390 245 234 198
180 177 156 105 96 93 70 57
57 31 27 25 21 18
NaeI GCC'GGC
Cuts at: 0 1162 2916 3109 4434
Size: 1162 1754 193 1325
Fragments arranged by size:
1754 1325 1162 193
NcoI C'CATG_G
Cuts at: 0 1118 4434
Size: 1118 3316
NgoMIV G'CCGG_C
Cuts at: 0 1160 2914 3107 4434
Size: 1160 1754 193 1327
Fragments arranged by size:
1754 1327 1160 193
NheI G'CTAG_C
Cuts at: 0 1113 1458 2310 4434
Size: 1113 345 852 2124
Fragments arranged by size:
2124 1113 852 345
NspI r_CATG'y
Cuts at: 0 759 953 1585 3990 4335 4434
Size: 759 194 632 2405 345 99
Fragments arranged by size:
2405 759 632 345 194 99
PciI A'CATG_T
Cuts at: 0 1581 4434
Size: 1581 2853
PflMI CCAn_nnn'nTGG
Cuts at: 0 348 398 1647 1902 2163 4166 4434
Size: 348 50 1249 255 261 2003 268
Fragments arranged by size:
2003 1249 348 268 261 255 50
PfoI T'CCnGG_A
Cuts at: 0 1364 1550 1901 2525 2789 2798 4214 4434
Size: 1364 186 351 624 264 9 1416 220
Fragments arranged by size:
1416 1364 624 351 264 220 186 9
PpuMI rG'GwC_Cy
Cuts at: 0 2235 3269 4251 4434
Size: 2235 1034 982 183
Fragments arranged by size:
2235 1034 982 183
PsiI TTA'TAA
Cuts at: 0 140 4434
Size: 140 4294
PspOMI G'GGCC_C
Cuts at: 0 2612 2963 3775 4434
Size: 2612 351 812 659
Fragments arranged by size:
2612 812 659 351
PspXI vC'TCGA_Gb
Cuts at: 0 1657 4434
Size: 1657 2777
PstI C_TGCA'G
Cuts at: 0 428 629 824 852 947 959 1034 1421
Size: 428 201 195 28 95 12 75 387
Cuts at: 1421 1547 1787 1928 2366 2567 2621 2864 2936
Size: 126 240 141 438 201 54 243 72
Cuts at: 2936 3119 3586 4434
Size: 183 467 848
Fragments arranged by size:
848 467 438 428 387 243 240 201
201 195 183 141 126 95 75 72
54 28 12
PvuII CAG'CTG
Cuts at: 0 765 796 1290 1308 1542 1563 2133 2226
Size: 765 31 494 18 234 21 570 93
Cuts at: 2226 2331 2487 2664 2691 2716 3018 3114 3560
Size: 105 156 177 27 25 302 96 446
Cuts at: 3560 4166 4434
Size: 606 268
Fragments arranged by size:
765 606 570 494 446 302 268 234
177 156 105 96 93 31 27 25
21 18
SacI G_AGCT'C
Cuts at: 0 290 442 893 1444 1841 2798 2999 4434
Size: 290 152 451 551 397 957 201 1435
Fragments arranged by size:
1435 957 551 451 397 290 201 152
SapI GCTCTTCn'nnn_
Cuts at: 0 306 564 1829 4434
Size: 306 258 1265 2605
Fragments arranged by size:
2605 1265 306 258
SbfI CC_TGCA'GG
Cuts at: 0 959 1787 1928 2621 2936 4434
Size: 959 828 141 693 315 1498
Fragments arranged by size:
1498 959 828 693 315 141
SfcI C'TryA_G
Cuts at: 0 424 625 820 848 943 955 1030 1417
Size: 424 201 195 28 95 12 75 387
Cuts at: 1417 1543 1783 1873 1924 2104 2362 2563 2617
Size: 126 240 90 51 180 258 201 54
Cuts at: 2617 2860 2932 3115 3582 3813 4119 4434
Size: 243 72 183 467 231 306 315
Fragments arranged by size:
467 424 387 315 306 258 243 240
231 201 201 195 183 180 126 95
90 75 72 54 51 28 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1999 2065 2584 4434
Size: 1999 66 519 1850
Fragments arranged by size:
1999 1850 519 66
SmaI CCC'GGG
Cuts at: 0 1324 4434
Size: 1324 3110
SmlI C'TyrA_G
Cuts at: 0 145 632 1051 1657 2197 2820 3007 3052
Size: 145 487 419 606 540 623 187 45
Cuts at: 3052 3094 3409 4306 4356 4434
Size: 42 315 897 50 78
Fragments arranged by size:
897 623 606 540 487 419 315 187
145 78 50 45 42
SphI G_CATG'C
Cuts at: 0 759 4434
Size: 759 3675
SrfI GCCC'GGGC
Cuts at: 0 1324 4434
Size: 1324 3110
StuI AGG'CCT
Cuts at: 0 1750 4154 4434
Size: 1750 2404 280
Fragments arranged by size:
2404 1750 280
StyI C'CwwG_G
Cuts at: 0 236 299 332 1037 1118 1244 1808 1976
Size: 236 63 33 705 81 126 564 168
Cuts at: 1976 2060 2903 3795 4434
Size: 84 843 892 639
Fragments arranged by size:
892 843 705 639 564 236 168 126
84 81 63 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 928 937 2008 2289 3169 3918 4434
Size: 928 9 1071 281 880 749 516
Fragments arranged by size:
1071 928 880 749 516 281 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1426 1458 2107 2139 4434
Size: 1426 32 649 32 2295
Fragments arranged by size:
2295 1426 649 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1857 2263 4434
Size: 1857 406 2171
Fragments arranged by size:
2171 1857 406
XhoI C'TCGA_G
Cuts at: 0 1657 2197 3409 4434
Size: 1657 540 1212 1025
Fragments arranged by size:
1657 1212 1025 540
XmaI C'CCGG_G
Cuts at: 0 1322 4434
Size: 1322 3112
XmnI GAAnn'nnTTC
Cuts at: 0 1172 4434
Size: 1172 3262
Enzymes that do cut:
AarI AclI AcuI AfeI AflII AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI
BanI BanII BbsI BbvCI BcgI BciVI BclI BglI
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI
CspCI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII MmeI
MscI MslI MspA1I NaeI NcoI NgoMIV NheI NspI
PciI PflMI PfoI PpuMI PsiI PspOMI PspXI PstI
PvuII SacI SapI SbfI SfcI SfiI SmaI SmlI
SphI SrfI StuI StyI TaqII TstI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AgeI AloI AscI AseI AsiSI
AvrII BbeI BfrBI BglII BmgBI BsaAI BsiEI BsiWI
BsmBI BspEI BsrGI BssHII BstBI BstZ17I BtgZI ClaI
DraI DraIII DrdI EagI EciI FseI FspAI HpaI
KasI KpnI MfeI MluI NarI NdeI NotI NruI
NsiI PacI PasI PmeI PmlI PpiI PshAI PsrI
PvuI RsrII SacII SalI SanDI ScaI SexAI SfoI
SgfI SgrAI SnaBI SpeI SspI SwaI TatI Tth111I
XbaI ZraI