Restriction map for
pFN21AE2632
(Linear) (Six-base) MAPSORT of: KIEE2632.seq Check: 9690 from: 1 to: 6219
KIEE2632.orf
With 182 enzymes: SgfI *
August 8, 2016 10:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3114 6219
Size: 3114 3105
AccI GT'mk_AC
Cuts at: 0 4436 4988 6219
Size: 4436 552 1231
Fragments arranged by size:
4436 1231 552
Acc65I G'GTAC_C
Cuts at: 0 1444 2153 6219
Size: 1444 709 4066
Fragments arranged by size:
4066 1444 709
AclI AA'CG_TT
Cuts at: 0 2446 6219
Size: 2446 3773
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 397 1639 2087 3014 3869 4508 5427
Size: 104 293 1242 448 927 855 639 919
Cuts at: 5427 6219
Size: 792
Fragments arranged by size:
1242 927 919 855 792 639 448 293
104
AflII C'TTAA_G
Cuts at: 0 783 6219
Size: 783 5436
AflIII A'CryG_T
Cuts at: 0 3985 6219
Size: 3985 2234
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3122 5218 5879 6219
Size: 3122 2096 661 340
Fragments arranged by size:
3122 2096 661 340
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3056 3088 5095 5127 6219
Size: 3056 32 2007 32 1092
Fragments arranged by size:
3056 2007 1092 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 142 453 821 2992 3183 5081 5295 5806
Size: 142 311 368 2171 191 1898 214 511
Cuts at: 5806 6219
Size: 413
Fragments arranged by size:
2171 1898 511 413 368 311 214 191
142
ApoI r'AATT_y
Cuts at: 0 748 2170 2254 2339 2393 3813 6219
Size: 748 1422 84 85 54 1420 2406
Fragments arranged by size:
2406 1422 1420 748 85 84 54
AvaI C'yCGr_G
Cuts at: 0 1686 5353 6219
Size: 1686 3667 866
Fragments arranged by size:
3667 1686 866
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 541 574 6219
Size: 541 33 5645
Fragments arranged by size:
5645 541 33
BamHI G'GATC_C
Cuts at: 0 1081 6219
Size: 1081 5138
BanI G'GyrC_C
Cuts at: 0 549 1222 1444 2153 6085 6219
Size: 549 673 222 709 3932 134
Fragments arranged by size:
3932 709 673 549 222 134
BanII G_rGCy'C
Cuts at: 0 1301 1513 3476 4466 6219
Size: 1301 212 1963 990 1753
Fragments arranged by size:
1963 1753 1301 990 212
BbsI GAAGACnn'nnnn_
Cuts at: 0 139 2147 3802 4205 6219
Size: 139 2008 1655 403 2014
Fragments arranged by size:
2014 2008 1655 403 139
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4548 4582 6219
Size: 4548 34 1637
Fragments arranged by size:
4548 1637 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 569 2515 6219
Size: 569 1946 3704
Fragments arranged by size:
3704 1946 569
BclI T'GATC_A
Cuts at: 0 1980 2576 4218 5484 6219
Size: 1980 596 1642 1266 735
Fragments arranged by size:
1980 1642 1266 735 596
BfrBI ATG'CAT
Cuts at: 0 1093 6219
Size: 1093 5126
BlpI GC'TnA_GC
Cuts at: 0 3023 6219
Size: 3023 3196
Bme1580I G_kGCm'C
Cuts at: 0 552 1586 2803 3993 6088 6219
Size: 552 1034 1217 1190 2095 131
Fragments arranged by size:
2095 1217 1190 1034 552 131
BmrI ACTGGGnnnn_n'
Cuts at: 0 469 557 1508 3899 3950 4789 6219
Size: 469 88 951 2391 51 839 1430
Fragments arranged by size:
2391 1430 951 839 469 88 51
BmtI G_CTAG'C
Cuts at: 0 3047 6219
Size: 3047 3172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2312 3205 3535 5115 6219
Size: 2312 893 330 1580 1104
Fragments arranged by size:
2312 1580 1104 893 330
Bpu10I CC'TnA_GC
Cuts at: 0 1591 6219
Size: 1591 4628
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 1167 3944 4679 5237 6219
Size: 373 794 2777 735 558 982
Fragments arranged by size:
2777 982 794 735 558 373
BsaI GGTCTCn'nnnn_
Cuts at: 0 1407 2669 3433 6219
Size: 1407 1262 764 2786
Fragments arranged by size:
2786 1407 1262 764
BsaHI Gr'CG_yC
Cuts at: 0 4433 6219
Size: 4433 1786
BsaWI w'CCGG_w
Cuts at: 0 1501 3799 6219
Size: 1501 2298 2420
Fragments arranged by size:
2420 2298 1501
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1530 1560 1789 1819 4115 4145 5095 5113
Size: 1530 30 229 30 2296 30 950 18
Cuts at: 5113 5125 5143 5643 5673 6219
Size: 12 18 500 30 546
Fragments arranged by size:
2296 1530 950 546 500 229 30 30
30 30 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 360 370 691 1791 1794 1797 2193 3232
Size: 360 10 321 1100 3 3 396 1039
Cuts at: 3232 3235 3238 3247 3250 3262 3289 3937 3940
Size: 3 3 9 3 12 27 648 3
Cuts at: 3940 4078 4081 4084 4087 4136 4243 4246 4525
Size: 138 3 3 3 49 107 3 279
Cuts at: 4525 5240 5645 5648 6219
Size: 715 405 3 571
Fragments arranged by size:
1100 1039 715 648 571 405 396 360
321 279 138 107 49 27 12 10
9 3 3 3 3 3 3 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 435 1814 2585 3050 4477 4593 5165 5413
Size: 435 1379 771 465 1427 116 572 248
Cuts at: 5413 5993 6089 6101 6219
Size: 580 96 12 118
Fragments arranged by size:
1427 1379 771 580 572 465 435 248
118 116 96 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 731 977 1596 2048 4190 4411 4751 5325
Size: 731 246 619 452 2142 221 340 574
Cuts at: 5325 5849 6219
Size: 524 370
Fragments arranged by size:
2142 731 619 574 524 452 370 340
246 221
BsiEI CG_ry'CG
Cuts at: 0 1033 6219
Size: 1033 5186
BsiHKAI G_wGCw'C
Cuts at: 0 5026 6219
Size: 5026 1193
BsiWI C'GTAC_G
Cuts at: 0 4830 6219
Size: 4830 1389
BsmI GAATG_Cn'
Cuts at: 0 568 808 898 923 1093 1095 3138 3574
Size: 568 240 90 25 170 2 2043 436
Cuts at: 3574 3651 6219
Size: 77 2568
Fragments arranged by size:
2568 2043 568 436 240 170 90 77
25 2
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2701 3445 5350 5795 6219
Size: 2701 744 1905 445 424
Fragments arranged by size:
2701 1905 744 445 424
Bsp1286I G_dGCh'C
Cuts at: 0 552 1301 1513 1586 2803 3476 3993 4466
Size: 552 749 212 73 1217 673 517 473
Cuts at: 4466 5026 6088 6219
Size: 560 1062 131
Fragments arranged by size:
1217 1062 749 673 560 552 517 473
212 131 73
BspHI T'CATG_A
Cuts at: 0 981 6219
Size: 981 5238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3114 3846 5242 6219
Size: 3114 732 1396 977
Fragments arranged by size:
3114 1396 977 732
BsrBI CCG'CTC
Cuts at: 0 3027 6219
Size: 3027 3192
BsrDI GCAATG_nn'
Cuts at: 0 315 2810 3633 5491 6219
Size: 315 2495 823 1858 728
Fragments arranged by size:
2495 1858 823 728 315
BsrFI r'CCGG_y
Cuts at: 0 2150 3152 6219
Size: 2150 1002 3067
Fragments arranged by size:
3067 2150 1002
BsrGI T'GTAC_A
Cuts at: 0 3818 6140 6219
Size: 3818 2322 79
Fragments arranged by size:
3818 2322 79
BssSI C'ACGA_G
Cuts at: 0 1352 6219
Size: 1352 4867
BstAPI GCAn_nnn'nTGC
Cuts at: 0 453 803 812 1890 5536 6111 6219
Size: 453 350 9 1078 3646 575 108
Fragments arranged by size:
3646 1078 575 453 350 108 9
BstEII G'GTnAC_C
Cuts at: 0 1109 6219
Size: 1109 5110
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1762 5340 6219
Size: 1762 3578 879
Fragments arranged by size:
3578 1762 879
BstYI r'GATC_y
Cuts at: 0 1081 4140 6219
Size: 1081 3059 2079
Fragments arranged by size:
3059 2079 1081
Bsu36I CC'TnA_GG
Cuts at: 0 4049 6219
Size: 4049 2170
BtgI C'CryG_G
Cuts at: 0 117 1736 3839 4187 4601 6126 6219
Size: 117 1619 2103 348 414 1525 93
Fragments arranged by size:
2103 1619 1525 414 348 117 93
BtsI GCAGTG_nn'
Cuts at: 0 799 822 1584 2993 3210 3432 6219
Size: 799 23 762 1409 217 222 2787
Fragments arranged by size:
2787 1409 799 762 222 217 23
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2292 2327 6219
Size: 2292 35 3892
Fragments arranged by size:
3892 2292 35
DraI TTT'AAA
Cuts at: 0 1842 3807 6219
Size: 1842 1965 2412
Fragments arranged by size:
2412 1965 1842
DraIII CAC_nnn'GTG
Cuts at: 0 1357 4018 6219
Size: 1357 2661 2201
Fragments arranged by size:
2661 2201 1357
DrdI GACnn_nn'nnGTC
Cuts at: 0 4432 6219
Size: 4432 1787
EaeI y'GGCC_r
Cuts at: 0 1535 2148 3154 3742 4821 6219
Size: 1535 613 1006 588 1079 1398
Fragments arranged by size:
1535 1398 1079 1006 613 588
EarI CTCTTCn'nnn_
Cuts at: 0 80 2063 2434 2640 2713 2993 3209 3239
Size: 80 1983 371 206 73 280 216 30
Cuts at: 3239 3251 3257 3266 3278 3488 3608 4049 4055
Size: 12 6 9 12 210 120 441 6
Cuts at: 4055 4073 4082 4091 4220 4232 4241 4319 5100
Size: 18 9 9 129 12 9 78 781
Cuts at: 5100 6219
Size: 1119
Fragments arranged by size:
1983 1119 781 441 371 280 216 210
206 129 120 80 78 73 30 18
12 12 12 9 9 9 9 6
6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 397 1639 2087 2312 3014 3205 3535
Size: 104 293 1242 448 225 702 191 330
Cuts at: 3535 3869 4508 5115 5427 6219
Size: 334 639 607 312 792
Fragments arranged by size:
1242 792 702 639 607 448 334 330
312 293 225 191 104
EcoO109I rG'GnC_Cy
Cuts at: 0 77 216 1931 4482 5611 5860 6219
Size: 77 139 1715 2551 1129 249 359
Fragments arranged by size:
2551 1715 1129 359 249 139 77
EcoRI G'AATT_C
Cuts at: 0 2254 6219
Size: 2254 3965
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1644 1971 6219
Size: 1644 327 4248
Fragments arranged by size:
4248 1644 327
HaeII r_GCGC'y
Cuts at: 0 237 5838 5923 6219
Size: 237 5601 85 296
Fragments arranged by size:
5601 296 237 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1066 1098 2600 2632 2852 2884 4942 4974
Size: 1066 32 1502 32 220 32 2058 32
Cuts at: 4974 5063 5095 5643 5675 6219
Size: 89 32 548 32 544
Fragments arranged by size:
2058 1502 1066 548 544 220 89 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1281 3080 4437 4842 5468 5937 6219
Size: 1281 1799 1357 405 626 469 282
Fragments arranged by size:
1799 1357 1281 626 469 405 282
HindIII A'AGCT_T
Cuts at: 0 2239 2539 4341 6219
Size: 2239 300 1802 1878
Fragments arranged by size:
2239 1878 1802 300
HpaI GTT'AAC
Cuts at: 0 1281 5468 6219
Size: 1281 4187 751
Fragments arranged by size:
4187 1281 751
KpnI G_GTAC'C
Cuts at: 0 1448 2157 6219
Size: 1448 709 4062
Fragments arranged by size:
4062 1448 709
MfeI C'AATT_G
Cuts at: 0 341 1847 6219
Size: 341 1506 4372
Fragments arranged by size:
4372 1506 341
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 2499 4760 5201 5254 6219
Size: 104 2395 2261 441 53 965
Fragments arranged by size:
2395 2261 965 441 104 53
MscI TGG'CCA
Cuts at: 0 3744 4823 6219
Size: 3744 1079 1396
Fragments arranged by size:
3744 1396 1079
MslI CAynn'nnrTG
Cuts at: 0 2750 4909 5770 5896 5902 5953 6178 6219
Size: 2750 2159 861 126 6 51 225 41
Fragments arranged by size:
2750 2159 861 225 126 51 41 6
MspA1I CmG'CkG
Cuts at: 0 150 450 1617 3180 3513 5108 5153 5186
Size: 150 300 1167 1563 333 1595 45 33
Cuts at: 5186 5295 5340 5512 5806 6219
Size: 109 45 172 294 413
Fragments arranged by size:
1595 1563 1167 413 333 300 294 172
150 109 45 45 33
NaeI GCC'GGC
Cuts at: 0 3154 6219
Size: 3154 3065
NcoI C'CATG_G
Cuts at: 0 117 3839 4601 6219
Size: 117 3722 762 1618
Fragments arranged by size:
3722 1618 762 117
NdeI CA'TA_TG
Cuts at: 0 887 6096 6219
Size: 887 5209 123
Fragments arranged by size:
5209 887 123
NgoMIV G'CCGG_C
Cuts at: 0 3152 6219
Size: 3152 3067
NheI G'CTAG_C
Cuts at: 0 3043 6219
Size: 3043 3176
NsiI A_TGCA'T
Cuts at: 0 1095 6219
Size: 1095 5124
NspI r_CATG'y
Cuts at: 0 815 2799 3989 5202 5991 6183 6219
Size: 815 1984 1190 1213 789 192 36
Fragments arranged by size:
1984 1213 1190 815 789 192 36
PasI CC'CwG_GG
Cuts at: 0 229 5605 6219
Size: 229 5376 614
Fragments arranged by size:
5376 614 229
PciI A'CATG_T
Cuts at: 0 3985 6219
Size: 3985 2234
PflMI CCAn_nnn'nTGG
Cuts at: 0 123 5295 6219
Size: 123 5172 924
Fragments arranged by size:
5172 924 123
PfoI T'CCnGG_A
Cuts at: 0 2432 6219
Size: 2432 3787
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1500 1532 5095 5127 6219
Size: 1500 32 3563 32 1092
Fragments arranged by size:
3563 1500 1092 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 216 1931 4482 5611 5860 6219
Size: 216 1715 2551 1129 249 359
Fragments arranged by size:
2551 1715 1129 359 249 216
PshAI GACnn'nnGTC
Cuts at: 0 1135 4739 6219
Size: 1135 3604 1480
Fragments arranged by size:
3604 1480 1135
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 5998 6030 6219
Size: 5998 32 189
Fragments arranged by size:
5998 189 32
PstI C_TGCA'G
Cuts at: 0 952 2828 2988 4925 5197 5207 5239 5462
Size: 952 1876 160 1937 272 10 32 223
Cuts at: 5462 6219
Size: 757
Fragments arranged by size:
1937 1876 952 757 272 223 160 32
10
PvuII CAG'CTG
Cuts at: 0 150 1617 3180 3513 5108 5153 5295 5340
Size: 150 1467 1563 333 1595 45 142 45
Cuts at: 5340 6219
Size: 879
Fragments arranged by size:
1595 1563 1467 879 333 150 142 45
45
RsrII CG'GwC_CG
Cuts at: 0 505 6219
Size: 505 5714
SalI G'TCGA_C
Cuts at: 0 4435 6219
Size: 4435 1784
SanDI GG'GwC_CC
Cuts at: 0 1931 4482 5611 6219
Size: 1931 2551 1129 608
Fragments arranged by size:
2551 1931 1129 608
SapI GCTCTTCn'nnn_
Cuts at: 0 2434 2713 2993 4241 4319 5100 6219
Size: 2434 279 280 1248 78 781 1119
Fragments arranged by size:
2434 1248 1119 781 280 279 78
ScaI AGT'ACT
Cuts at: 0 111 3464 4355 6219
Size: 111 3353 891 1864
Fragments arranged by size:
3353 1864 891 111
SexAI A'CCwGG_T
Cuts at: 0 1113 2194 4014 6219
Size: 1113 1081 1820 2205
Fragments arranged by size:
2205 1820 1113 1081
SfcI C'TryA_G
Cuts at: 0 822 948 1439 2824 2984 4704 4714 4921
Size: 822 126 491 1385 160 1720 10 207
Cuts at: 4921 5115 5193 5203 5235 5458 5846 6219
Size: 194 78 10 32 223 388 373
Fragments arranged by size:
1720 1385 822 491 388 373 223 207
194 160 126 78 32 10 10
SmlI C'TyrA_G
Cuts at: 0 352 783 1182 3959 4694 5252 6219
Size: 352 431 399 2777 735 558 967
Fragments arranged by size:
2777 967 735 558 431 399 352
SphI G_CATG'C
Cuts at: 0 5202 6219
Size: 5202 1017
SspI AAT'ATT
Cuts at: 0 2286 3133 3315 5712 6219
Size: 2286 847 182 2397 507
Fragments arranged by size:
2397 2286 847 507 182
StuI AGG'CCT
Cuts at: 0 200 781 3375 4702 5274 6219
Size: 200 581 2594 1327 572 945
Fragments arranged by size:
2594 1327 945 581 572 200
StyI C'CwwG_G
Cuts at: 0 117 275 831 1390 3839 4466 4601 6219
Size: 117 158 556 559 2449 627 135 1618
Fragments arranged by size:
2449 1618 627 559 556 158 135 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 749 1047 1716 3852 5179 5346 5827 6103
Size: 749 298 669 2136 1327 167 481 276
Cuts at: 6103 6219
Size: 116
Fragments arranged by size:
2136 1327 749 669 481 298 276 167
116
TatI w'GTAC_w
Cuts at: 0 109 512 3114 3381 3462 3818 4353 6140
Size: 109 403 2602 267 81 356 535 1787
Cuts at: 6140 6219
Size: 79
Fragments arranged by size:
2602 1787 535 403 356 267 109 81
79
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1530 1562 1789 1821 5585 5617 6219
Size: 1530 32 227 32 3764 32 602
Fragments arranged by size:
3764 1530 602 227 32 32 32
XbaI T'CTAG_A
Cuts at: 0 283 2641 6219
Size: 283 2358 3578
Fragments arranged by size:
3578 2358 283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 929 2430 3586 4816 4944 6025 6219
Size: 929 1501 1156 1230 128 1081 194
Fragments arranged by size:
1501 1230 1156 1081 929 194 128
XmnI GAAnn'nnTTC
Cuts at: 0 1093 3314 6219
Size: 1093 2221 2905
Fragments arranged by size:
2905 2221 1093
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AlfI
AloI AlwNI ApoI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BlpI Bme1580I BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI
DraI DraIII DrdI EaeI EarI Eco57MI EcoO109I EcoRI
FalI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NsiI NspI PasI PciI PflMI PfoI PpiI
PpuMI PshAI PsrI PstI PvuII RsrII SalI SanDI
SapI ScaI SexAI SfcI SmlI SphI SspI StuI
StyI TaqII TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AleI ApaI ApaLI AscI
AseI AsiSI AvrII BbeI BbvCI BglI BglII BmgBI
BplI BsaAI BsaBI BspEI BssHII BstBI BstZ17I BtgZI
ClaI EagI EciI EcoICRI EcoNI EcoRV FseI FspI
FspAI KasI MluI NarI NotI NruI PacI PmeI
PmlI PsiI PspOMI PspXI PvuI SacI SacII SbfI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SwaI Tth111I XhoI XmaI ZraI