Restriction map for
pFN21AE2629
(Linear) (Six-base) MAPSORT of: KIEE2629.seq Check: 7195 from: 1 to: 2850
KIEE2629.orf
With 182 enzymes: SgfI *
June 10, 2016 16:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1014 1209 2029 2615 2850
Size: 1014 195 820 586 235
Fragments arranged by size:
1014 820 586 235 195
AccI GT'mk_AC
Cuts at: 0 191 2850
Size: 191 2659
Acc65I G'GTAC_C
Cuts at: 0 2001 2850
Size: 2001 849
AleI CACnn'nnGTG
Cuts at: 0 2660 2850
Size: 2660 190
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 343 2179 2850
Size: 343 1836 671
Fragments arranged by size:
1836 671 343
AlwNI CAG_nnn'CTG
Cuts at: 0 657 872 1557 2607 2616 2652 2796 2850
Size: 657 215 685 1050 9 36 144 54
Fragments arranged by size:
1050 685 657 215 144 54 36 9
ApaI G_GGCC'C
Cuts at: 0 73 2813 2850
Size: 73 2740 37
Fragments arranged by size:
2740 73 37
ApaLI G'TGCA_C
Cuts at: 0 1993 2850
Size: 1993 857
AvaI C'yCGr_G
Cuts at: 0 25 160 176 360 415 2105 2850
Size: 25 135 16 184 55 1690 745
Fragments arranged by size:
1690 745 184 135 55 25 16
AvrII C'CTAG_G
Cuts at: 0 744 2850
Size: 744 2106
BamHI G'GATC_C
Cuts at: 0 12 154 2474 2850
Size: 12 142 2320 376
Fragments arranged by size:
2320 376 142 12
BanI G'GyrC_C
Cuts at: 0 125 261 405 1151 1319 1494 2001 2188
Size: 125 136 144 746 168 175 507 187
Cuts at: 2188 2313 2700 2850
Size: 125 387 150
Fragments arranged by size:
746 507 387 187 175 168 150 144
136 125 125
BanII G_rGCy'C
Cuts at: 0 49 73 88 313 361 1622 1755 2402
Size: 49 24 15 225 48 1261 133 647
Cuts at: 2402 2486 2723 2813 2850
Size: 84 237 90 37
Fragments arranged by size:
1261 647 237 225 133 90 84 49
48 37 24 15
BbeI G_GCGC'C
Cuts at: 0 129 1323 1498 2850
Size: 129 1194 175 1352
Fragments arranged by size:
1352 1194 175 129
BbsI GAAGACnn'nnnn_
Cuts at: 0 2379 2850
Size: 2379 471
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1572 1606 2850
Size: 1572 34 1244
Fragments arranged by size:
1572 1244 34
BglI GCCn_nnn'nGGC
Cuts at: 0 44 717 1267 1279 1483 2307 2780 2850
Size: 44 673 550 12 204 824 473 70
Fragments arranged by size:
824 673 550 473 204 70 44 12
BlpI GC'TnA_GC
Cuts at: 0 611 1105 1871 2850
Size: 611 494 766 979
Fragments arranged by size:
979 766 611 494
Bme1580I G_kGCm'C
Cuts at: 0 73 266 1952 1997 2191 2813 2850
Size: 73 193 1686 45 194 622 37
Fragments arranged by size:
1686 622 194 193 73 45 37
BmgBI CAC'GTC
Cuts at: 0 135 2202 2850
Size: 135 2067 648
Fragments arranged by size:
2067 648 135
BmrI ACTGGGnnnn_n'
Cuts at: 0 52 1188 1381 1659 1797 2850
Size: 52 1136 193 278 138 1053
Fragments arranged by size:
1136 1053 278 193 138 52
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 361 649 976 985 1198 1306 1417
Size: 79 282 288 327 9 213 108 111
Cuts at: 1417 1441 1459 1606 1984 2047 2500 2707 2850
Size: 24 18 147 378 63 453 207 143
Fragments arranged by size:
453 378 327 288 282 213 207 147
143 111 108 79 63 24 18 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1747 2386 2850
Size: 1747 639 464
Fragments arranged by size:
1747 639 464
BsaI GGTCTCn'nnnn_
Cuts at: 0 1501 2632 2850
Size: 1501 1131 218
Fragments arranged by size:
1501 1131 218
BsaAI yAC'GTr
Cuts at: 0 2560 2850
Size: 2560 290
BsaHI Gr'CG_yC
Cuts at: 0 126 900 1320 1495 1577 2582 2850
Size: 126 774 420 175 82 1005 268
Fragments arranged by size:
1005 774 420 268 175 126 82
BsaWI w'CCGG_w
Cuts at: 0 237 1706 2850
Size: 237 1469 1144
Fragments arranged by size:
1469 1144 237
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 982 1012 1144 1174 1432 1462 2850
Size: 982 30 132 30 258 30 1388
Fragments arranged by size:
1388 982 258 132 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 982 985 988 1225 1732 2704 2731 2850
Size: 982 3 3 237 507 972 27 119
Fragments arranged by size:
982 972 507 237 119 27 3 3
BseYI C'CCAG_C
Cuts at: 0 316 662 921 1010 1464 1851 1889 2145
Size: 316 346 259 89 454 387 38 256
Cuts at: 2145 2150 2416 2600 2749 2778 2812 2850
Size: 5 266 184 149 29 34 38
Fragments arranged by size:
454 387 346 316 266 259 256 184
149 89 38 38 34 29 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 889 947 1612 1879 2063 2638 2850
Size: 889 58 665 267 184 575 212
Fragments arranged by size:
889 665 575 267 212 184 58
BsiEI CG_ry'CG
Cuts at: 0 1256 1370 1478 1525 2661 2850
Size: 1256 114 108 47 1136 189
Fragments arranged by size:
1256 1136 189 114 108 47
BsiHKAI G_wGCw'C
Cuts at: 0 1706 1808 1997 2090 2198 2402 2486 2565
Size: 1706 102 189 93 108 204 84 79
Cuts at: 2565 2850
Size: 285
Fragments arranged by size:
1706 285 204 189 108 102 93 84
79
BsmBI CGTCTCn'nnnn_
Cuts at: 0 781 2278 2773 2850
Size: 781 1497 495 77
Fragments arranged by size:
1497 781 495 77
Bsp1286I G_dGCh'C
Cuts at: 0 49 73 88 266 313 361 1622 1706
Size: 49 24 15 178 47 48 1261 84
Cuts at: 1706 1755 1808 1952 1997 2090 2191 2198 2402
Size: 49 53 144 45 93 101 7 204
Cuts at: 2402 2486 2565 2723 2813 2850
Size: 84 79 158 90 37
Fragments arranged by size:
1261 204 178 158 144 101 93 90
84 84 79 53 49 49 48 47
45 37 24 15 7
BspEI T'CCGG_A
Cuts at: 0 237 2850
Size: 237 2613
BspHI T'CATG_A
Cuts at: 0 2018 2850
Size: 2018 832
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 598 840 1014 1209 2029 2471 2507 2615
Size: 598 242 174 195 820 442 36 108
Cuts at: 2615 2850
Size: 235
Fragments arranged by size:
820 598 442 242 235 195 174 108
36
BsrDI GCAATG_nn'
Cuts at: 0 2163 2850
Size: 2163 687
BsrFI r'CCGG_y
Cuts at: 0 635 1291 1369 1470 2850
Size: 635 656 78 101 1380
Fragments arranged by size:
1380 656 635 101 78
BssHII G'CGCG_C
Cuts at: 0 1433 1528 1606 2850
Size: 1433 95 78 1244
Fragments arranged by size:
1433 1244 95 78
BssSI C'ACGA_G
Cuts at: 0 1633 2564 2850
Size: 1633 931 286
Fragments arranged by size:
1633 931 286
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1902 2652 2850
Size: 1902 750 198
Fragments arranged by size:
1902 750 198
BstEII G'GTnAC_C
Cuts at: 0 291 2850
Size: 291 2559
BstXI CCAn_nnnn'nTGG
Cuts at: 0 57 670 1866 2310 2850
Size: 57 613 1196 444 540
Fragments arranged by size:
1196 613 540 444 57
BstYI r'GATC_y
Cuts at: 0 12 154 2474 2850
Size: 12 142 2320 376
Fragments arranged by size:
2320 376 142 12
BtgI C'CryG_G
Cuts at: 0 252 910 1131 1459 1911 2661 2850
Size: 252 658 221 328 452 750 189
Fragments arranged by size:
750 658 452 328 252 221 189
BtsI GCAGTG_nn'
Cuts at: 0 935 1099 2051 2736 2850
Size: 935 164 952 685 114
Fragments arranged by size:
952 935 685 164 114
ClaI AT'CG_AT
Cuts at: 0 2804 2850
Size: 2804 46
DraIII CAC_nnn'GTG
Cuts at: 0 1090 1635 2202 2850
Size: 1090 545 567 648
Fragments arranged by size:
1090 648 567 545
EaeI y'GGCC_r
Cuts at: 0 255 549 1052 1253 1268 1367 1475 2568
Size: 255 294 503 201 15 99 108 1093
Cuts at: 2568 2850
Size: 282
Fragments arranged by size:
1093 503 294 282 255 201 108 99
15
EagI C'GGCC_G
Cuts at: 0 1253 1367 1475 2850
Size: 1253 114 108 1375
Fragments arranged by size:
1375 1253 114 108
EarI CTCTTCn'nnn_
Cuts at: 0 59 1873 2850
Size: 59 1814 977
Fragments arranged by size:
1814 977 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 301 2761 2850
Size: 301 2460 89
Fragments arranged by size:
2460 301 89
EcoICRI GAG'CTC
Cuts at: 0 2400 2484 2850
Size: 2400 84 366
Fragments arranged by size:
2400 366 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 361 649 976 985 1198 1306 1417
Size: 79 282 288 327 9 213 108 111
Cuts at: 1417 1441 1459 1606 1984 2047 2500 2707 2850
Size: 24 18 147 378 63 453 207 143
Fragments arranged by size:
453 378 327 288 282 213 207 147
143 111 108 79 63 24 18 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 988 1207 1540 2788 2850
Size: 988 219 333 1248 62
Fragments arranged by size:
1248 988 333 219 62
EcoO109I rG'GnC_Cy
Cuts at: 0 22 54 69 70 474 544 831 2613
Size: 22 32 15 1 404 70 287 1782
Cuts at: 2613 2649 2694 2793 2850
Size: 36 45 99 57
Fragments arranged by size:
1782 404 287 99 70 57 45 36
32 22 15 1
EcoRV GAT'ATC
Cuts at: 0 843 2850
Size: 843 2007
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 250 2850
Size: 250 2600
FseI GG_CCGG'CC
Cuts at: 0 1295 2850
Size: 1295 1555
HaeII r_GCGC'y
Cuts at: 0 129 879 952 1189 1323 1498 1571 2850
Size: 129 750 73 237 134 175 73 1279
Fragments arranged by size:
1279 750 237 175 134 129 73 73
HincII GTy'rAC
Cuts at: 0 2496 2850
Size: 2496 354
KasI G'GCGC_C
Cuts at: 0 125 1319 1494 2850
Size: 125 1194 175 1356
Fragments arranged by size:
1356 1194 175 125
KpnI G_GTAC'C
Cuts at: 0 2005 2850
Size: 2005 845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1060 2273 2850
Size: 1060 1213 577
Fragments arranged by size:
1213 1060 577
MscI TGG'CCA
Cuts at: 0 2570 2850
Size: 2570 280
MslI CAynn'nnrTG
Cuts at: 0 1910 2660 2850
Size: 1910 750 190
Fragments arranged by size:
1910 750 190
MspA1I CmG'CkG
Cuts at: 0 254 912 1014 1133 1366 1461 1500 1554
Size: 254 658 102 119 233 95 39 54
Cuts at: 1554 1626 1855 1893 2354 2850
Size: 72 229 38 461 496
Fragments arranged by size:
658 496 461 254 233 229 119 102
95 72 54 39 38
NaeI GCC'GGC
Cuts at: 0 1293 1371 1472 2850
Size: 1293 78 101 1378
Fragments arranged by size:
1378 1293 101 78
NarI GG'CG_CC
Cuts at: 0 126 1320 1495 2850
Size: 126 1194 175 1355
Fragments arranged by size:
1355 1194 175 126
NcoI C'CATG_G
Cuts at: 0 1911 2850
Size: 1911 939
NgoMIV G'CCGG_C
Cuts at: 0 1291 1369 1470 2850
Size: 1291 78 101 1380
Fragments arranged by size:
1380 1291 101 78
NspI r_CATG'y
Cuts at: 0 372 2301 2850
Size: 372 1929 549
Fragments arranged by size:
1929 549 372
PasI CC'CwG_GG
Cuts at: 0 74 469 834 2850
Size: 74 395 365 2016
Fragments arranged by size:
2016 395 365 74
PflMI CCAn_nnn'nTGG
Cuts at: 0 135 2140 2577 2796 2850
Size: 135 2005 437 219 54
Fragments arranged by size:
2005 437 219 135 54
PfoI T'CCnGG_A
Cuts at: 0 485 2095 2477 2850
Size: 485 1610 382 373
Fragments arranged by size:
1610 485 382 373
PmlI CAC'GTG
Cuts at: 0 2560 2850
Size: 2560 290
PpuMI rG'GwC_Cy
Cuts at: 0 22 54 2850
Size: 22 32 2796
Fragments arranged by size:
2796 32 22
PspOMI G'GGCC_C
Cuts at: 0 69 2809 2850
Size: 69 2740 41
Fragments arranged by size:
2740 69 41
PstI C_TGCA'G
Cuts at: 0 212 224 851 1846 2612 2648 2731 2850
Size: 212 12 627 995 766 36 83 119
Fragments arranged by size:
995 766 627 212 119 83 36 12
PvuII CAG'CTG
Cuts at: 0 1014 1554 1626 1855 1893 2850
Size: 1014 540 72 229 38 957
Fragments arranged by size:
1014 957 540 229 72 38
SacI G_AGCT'C
Cuts at: 0 2402 2486 2850
Size: 2402 84 364
Fragments arranged by size:
2402 364 84
SacII CC_GC'GG
Cuts at: 0 255 913 1134 1462 2850
Size: 255 658 221 328 1388
Fragments arranged by size:
1388 658 328 255 221
SanDI GG'GwC_CC
Cuts at: 0 22 2850
Size: 22 2828
SapI GCTCTTCn'nnn_
Cuts at: 0 1873 2850
Size: 1873 977
SbfI CC_TGCA'GG
Cuts at: 0 851 2612 2648 2850
Size: 851 1761 36 202
Fragments arranged by size:
1761 851 202 36
SexAI A'CCwGG_T
Cuts at: 0 1997 2207 2850
Size: 1997 210 643
Fragments arranged by size:
1997 643 210
SfcI C'TryA_G
Cuts at: 0 198 208 220 847 1842 2608 2644 2727
Size: 198 10 12 627 995 766 36 83
Cuts at: 2727 2850
Size: 123
Fragments arranged by size:
995 766 627 198 123 83 36 12
10
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1267 2850
Size: 1267 1583
SfoI GGC'GCC
Cuts at: 0 127 1321 1496 2850
Size: 127 1194 175 1354
Fragments arranged by size:
1354 1194 175 127
SmaI CCC'GGG
Cuts at: 0 178 362 417 2850
Size: 178 184 55 2433
Fragments arranged by size:
2433 184 178 55
SmlI C'TyrA_G
Cuts at: 0 1762 2401 2850
Size: 1762 639 449
Fragments arranged by size:
1762 639 449
SphI G_CATG'C
Cuts at: 0 2301 2850
Size: 2301 549
SrfI GCCC'GGGC
Cuts at: 0 178 2850
Size: 178 2672
StuI AGG'CCT
Cuts at: 0 533 2850
Size: 533 2317
StyI C'CwwG_G
Cuts at: 0 50 449 744 1911 2850
Size: 50 399 295 1167 939
Fragments arranged by size:
1167 939 399 295 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 826 913 1903 2038 2147 2440 2850
Size: 826 87 990 135 109 293 410
Fragments arranged by size:
990 826 410 293 135 109 87
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 261 293 1074 1106 1144 1176 1432 1464
Size: 261 32 781 32 38 32 256 32
Cuts at: 1464 1645 1677 2850
Size: 181 32 1173
Fragments arranged by size:
1173 781 261 256 181 38 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 496 2850
Size: 496 2354
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 522 2137 2169 2850
Size: 522 1615 32 681
Fragments arranged by size:
1615 681 522 32
XmaI C'CCGG_G
Cuts at: 0 176 360 415 2850
Size: 176 184 55 2435
Fragments arranged by size:
2435 184 176 55
Enzymes that do cut:
AarI AccI Acc65I AleI AlfI AlwNI ApaI ApaLI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BglI BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI
BssHII BssSI BstAPI BstEII BstXI BstYI BtgI BtsI
ClaI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV FalI FseI HaeII HincII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PflMI PfoI PmlI PpuMI PspOMI
PstI PvuII SacI SacII SanDI SapI SbfI SexAI
SfcI SfiI SfoI SmaI SmlI SphI SrfI StuI
StyI TaqII TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AclI AcuI AfeI AflII AflIII AgeI AhdI
AloI ApoI AscI AseI AsiSI BaeI BbvCI BciVI
BclI BfrBI BglII BmtI BplI Bpu10I BsaBI BsiWI
BsmI BsrBI BsrGI BstBI BstZ17I Bsu36I BtgZI CspCI
DraI DrdI EcoRI FspI FspAI Hin4I HindIII HpaI
MfeI MluI NdeI NheI NotI NruI NsiI PacI
PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SalI ScaI SgfI SgrAI SnaBI SpeI SspI
SwaI TatI XbaI XhoI XmnI ZraI