Restriction map for
pFN21AE2625
(Linear) (Six-base) MAPSORT of: KIEE2625.seq Check: 9500 from: 1 to: 1338
KIEE2625.orf
With 182 enzymes: SgfI *
May 20, 2016 17:05 ..
AatII G_ACGT'C
Cuts at: 0 179 1338
Size: 179 1159
AccI GT'mk_AC
Cuts at: 0 179 801 1338
Size: 179 622 537
Fragments arranged by size:
622 537 179
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 1157 1338
Size: 476 681 181
Fragments arranged by size:
681 476 181
AflIII A'CryG_T
Cuts at: 0 251 1338
Size: 251 1087
AvaI C'yCGr_G
Cuts at: 0 27 1338
Size: 27 1311
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 936 969 1338
Size: 936 33 369
Fragments arranged by size:
936 369 33
BamHI G'GATC_C
Cuts at: 0 1029 1338
Size: 1029 309
BanI G'GyrC_C
Cuts at: 0 1185 1338
Size: 1185 153
BbsI GAAGACnn'nnnn_
Cuts at: 0 403 1338
Size: 403 935
BfrBI ATG'CAT
Cuts at: 0 894 1338
Size: 894 444
BglII A'GATC_T
Cuts at: 0 278 1338
Size: 278 1060
BlpI GC'TnA_GC
Cuts at: 0 49 1338
Size: 49 1289
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 282 732 843 1338
Size: 186 96 450 111 495
Fragments arranged by size:
495 450 186 111 96
Bpu10I CC'TnA_GC
Cuts at: 0 1083 1338
Size: 1083 255
BsaHI Gr'CG_yC
Cuts at: 0 176 1338
Size: 176 1162
BsaWI w'CCGG_w
Cuts at: 0 674 934 1338
Size: 674 260 404
Fragments arranged by size:
674 404 260
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 280 310 1037 1067 1338
Size: 280 30 727 30 271
Fragments arranged by size:
727 280 271 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1058 1235 1338
Size: 1058 177 103
Fragments arranged by size:
1058 177 103
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 522 902 1338
Size: 60 462 380 436
Fragments arranged by size:
462 436 380 60
BsiEI CG_ry'CG
Cuts at: 0 1151 1338
Size: 1151 187
BsiHKAI G_wGCw'C
Cuts at: 0 441 1338
Size: 441 897
BsmBI CGTCTCn'nnnn_
Cuts at: 0 754 1338
Size: 754 584
Bsp1286I G_dGCh'C
Cuts at: 0 441 1338
Size: 441 897
BspEI T'CCGG_A
Cuts at: 0 674 934 1338
Size: 674 260 404
Fragments arranged by size:
674 404 260
BsrDI GCAATG_nn'
Cuts at: 0 168 1338
Size: 168 1170
BsrFI r'CCGG_y
Cuts at: 0 326 1338
Size: 326 1012
BsrGI T'GTAC_A
Cuts at: 0 1019 1338
Size: 1019 319
BstAPI GCAn_nnn'nTGC
Cuts at: 0 336 1338
Size: 336 1002
BstEII G'GTnAC_C
Cuts at: 0 789 1338
Size: 789 549
BstXI CCAn_nnnn'nTGG
Cuts at: 0 210 1338
Size: 210 1128
BstYI r'GATC_y
Cuts at: 0 278 1029 1338
Size: 278 751 309
Fragments arranged by size:
751 309 278
Bsu36I CC'TnA_GG
Cuts at: 0 588 1034 1338
Size: 588 446 304
Fragments arranged by size:
588 446 304
BtgI C'CryG_G
Cuts at: 0 14 153 238 979 1181 1305 1338
Size: 14 139 85 741 202 124 33
Fragments arranged by size:
741 202 139 124 85 33 14
BtsI GCAGTG_nn'
Cuts at: 0 914 1338
Size: 914 424
DrdI GACnn_nn'nnGTC
Cuts at: 0 1262 1338
Size: 1262 76
EarI CTCTTCn'nnn_
Cuts at: 0 202 347 803 1091 1338
Size: 202 145 456 288 247
Fragments arranged by size:
456 288 247 202 145
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 282 476 732 843 1157 1338
Size: 186 96 194 256 111 314 181
Fragments arranged by size:
314 256 194 186 181 111 96
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 506 1187 1338
Size: 506 681 151
Fragments arranged by size:
681 506 151
HaeII r_GCGC'y
Cuts at: 0 49 195 1216 1338
Size: 49 146 1021 122
Fragments arranged by size:
1021 146 122 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 64 96 505 537 1037 1069 1338
Size: 64 32 409 32 500 32 269
Fragments arranged by size:
500 409 269 64 32 32 32
HincII GTy'rAC
Cuts at: 0 802 1338
Size: 802 536
MfeI C'AATT_G
Cuts at: 0 89 1338
Size: 89 1249
MslI CAynn'nnrTG
Cuts at: 0 634 1338
Size: 634 704
MspA1I CmG'CkG
Cuts at: 0 16 53 141 1338
Size: 16 37 88 1197
Fragments arranged by size:
1197 88 37 16
NsiI A_TGCA'T
Cuts at: 0 896 1338
Size: 896 442
PfoI T'CCnGG_A
Cuts at: 0 170 404 477 1338
Size: 170 234 73 861
Fragments arranged by size:
861 234 170 73
PvuII CAG'CTG
Cuts at: 0 53 141 1338
Size: 53 88 1197
Fragments arranged by size:
1197 88 53
SacII CC_GC'GG
Cuts at: 0 17 1338
Size: 17 1321
SalI G'TCGA_C
Cuts at: 0 800 1338
Size: 800 538
SapI GCTCTTCn'nnn_
Cuts at: 0 803 1338
Size: 803 535
SmaI CCC'GGG
Cuts at: 0 29 1338
Size: 29 1309
TatI w'GTAC_w
Cuts at: 0 1019 1338
Size: 1019 319
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 210 465 1075 1338
Size: 210 255 610 263
Fragments arranged by size:
610 263 255 210
XmaI C'CCGG_G
Cuts at: 0 27 1338
Size: 27 1311
XmnI GAAnn'nnTTC
Cuts at: 0 1229 1338
Size: 1229 109
ZraI GAC'GTC
Cuts at: 0 177 1338
Size: 177 1161
Enzymes that do cut:
AatII AccI AcuI AflIII AvaI BaeI BamHI BanI
BbsI BfrBI BglII BlpI BpmI Bpu10I BsaHI BsaWI
BsaXI BseRI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BsrDI BsrFI BsrGI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtsI DrdI EarI Eco57MI FalI HaeII Hin4I
HincII MfeI MslI MspA1I NsiI PfoI PvuII SacII
SalI SapI SmaI TatI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BanII BbeI BbvCI BcgI BciVI BclI
BglI Bme1580I BmgBI BmrI BmtI BplI BpuEI BsaI
BsaAI BsaBI BseYI BsiWI BsmI BspHI BspMI BsrBI
BssHII BssSI BstBI BstZ17I BtgZI ClaI CspCI DraI
DraIII EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRI
EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI
MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspI PacI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SanDI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TaqII
TstI Tth111I XbaI XhoI