Restriction map for
pFN21AE2624
(Linear) (Six-base) MAPSORT of: KIEE2624.seq Check: 645 from: 1 to: 780
KIEE2624.orf
With 182 enzymes: SgfI *
May 26, 2016 12:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 581 780
Size: 581 199
AlwNI CAG_nnn'CTG
Cuts at: 0 336 609 690 780
Size: 336 273 81 90
Fragments arranged by size:
336 273 90 81
ApaI G_GGCC'C
Cuts at: 0 105 165 602 780
Size: 105 60 437 178
Fragments arranged by size:
437 178 105 60
ApoI r'AATT_y
Cuts at: 0 562 780
Size: 562 218
AscI GG'CGCG_CC
Cuts at: 0 174 780
Size: 174 606
AvaI C'yCGr_G
Cuts at: 0 774 780
Size: 774 6
BanI G'GyrC_C
Cuts at: 0 35 228 241 291 405 780
Size: 35 193 13 50 114 375
Fragments arranged by size:
375 193 114 50 35 13
BanII G_rGCy'C
Cuts at: 0 105 165 386 602 780
Size: 105 60 221 216 178
Fragments arranged by size:
221 216 178 105 60
BbeI G_GCGC'C
Cuts at: 0 39 232 295 409 780
Size: 39 193 63 114 371
Fragments arranged by size:
371 193 114 63 39
BglI GCCn_nnn'nGGC
Cuts at: 0 10 780
Size: 10 770
Bme1580I G_kGCm'C
Cuts at: 0 105 165 602 780
Size: 105 60 437 178
Fragments arranged by size:
437 178 105 60
BmgBI CAC'GTC
Cuts at: 0 495 780
Size: 495 285
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 780
Size: 283 497
Bpu10I CC'TnA_GC
Cuts at: 0 739 780
Size: 739 41
BsaI GGTCTCn'nnnn_
Cuts at: 0 613 780
Size: 613 167
BsaHI Gr'CG_yC
Cuts at: 0 36 229 268 292 406 416 780
Size: 36 193 39 24 114 10 364
Fragments arranged by size:
364 193 114 39 36 24 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 33 265 712 780
Size: 33 232 447 68
Fragments arranged by size:
447 232 68 33
BseYI C'CCAG_C
Cuts at: 0 90 198 232 522 602 780
Size: 90 108 34 290 80 178
Fragments arranged by size:
290 178 108 90 80 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 553 780
Size: 349 204 227
Fragments arranged by size:
349 227 204
BsiEI CG_ry'CG
Cuts at: 0 169 483 780
Size: 169 314 297
Fragments arranged by size:
314 297 169
BsiHKAI G_wGCw'C
Cuts at: 0 665 780
Size: 665 115
BsmI GAATG_Cn'
Cuts at: 0 612 780
Size: 612 168
Bsp1286I G_dGCh'C
Cuts at: 0 105 165 386 602 665 780
Size: 105 60 221 216 63 115
Fragments arranged by size:
221 216 115 105 63 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 461 581 693 780
Size: 461 120 112 87
Fragments arranged by size:
461 120 112 87
BsrBI CCG'CTC
Cuts at: 0 630 780
Size: 630 150
BsrFI r'CCGG_y
Cuts at: 0 294 576 780
Size: 294 282 204
Fragments arranged by size:
294 282 204
BssHII G'CGCG_C
Cuts at: 0 107 174 780
Size: 107 67 606
Fragments arranged by size:
606 107 67
BstAPI GCAn_nnn'nTGC
Cuts at: 0 336 708 780
Size: 336 372 72
Fragments arranged by size:
372 336 72
BtgI C'CryG_G
Cuts at: 0 5 354 780
Size: 5 349 426
Fragments arranged by size:
426 349 5
EaeI y'GGCC_r
Cuts at: 0 2 166 296 357 480 780
Size: 2 164 130 61 123 300
Fragments arranged by size:
300 164 130 123 61 2
EagI C'GGCC_G
Cuts at: 0 166 480 780
Size: 166 314 300
Fragments arranged by size:
314 300 166
EarI CTCTTCn'nnn_
Cuts at: 0 640 780
Size: 640 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 166 780
Size: 166 614
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 780
Size: 283 497
EcoNI CCTnn'n_nnAGG
Cuts at: 0 433 742 780
Size: 433 309 38
Fragments arranged by size:
433 309 38
EcoO109I rG'GnC_Cy
Cuts at: 0 95 161 275 486 527 599 771 780
Size: 95 66 114 211 41 72 172 9
Fragments arranged by size:
211 172 114 95 72 66 41 9
EcoRI G'AATT_C
Cuts at: 0 562 780
Size: 562 218
HaeII r_GCGC'y
Cuts at: 0 39 232 259 295 409 691 780
Size: 39 193 27 36 114 282 89
Fragments arranged by size:
282 193 114 89 39 36 27
HincII GTy'rAC
Cuts at: 0 375 780
Size: 375 405
KasI G'GCGC_C
Cuts at: 0 35 228 291 405 780
Size: 35 193 63 114 375
Fragments arranged by size:
375 193 114 63 35
MspA1I CmG'CkG
Cuts at: 0 7 181 464 609 780
Size: 7 174 283 145 171
Fragments arranged by size:
283 174 171 145 7
NaeI GCC'GGC
Cuts at: 0 296 578 780
Size: 296 282 202
Fragments arranged by size:
296 282 202
NarI GG'CG_CC
Cuts at: 0 36 229 292 406 780
Size: 36 193 63 114 374
Fragments arranged by size:
374 193 114 63 36
NgoMIV G'CCGG_C
Cuts at: 0 294 576 780
Size: 294 282 204
Fragments arranged by size:
294 282 204
PflMI CCAn_nnn'nTGG
Cuts at: 0 756 780
Size: 756 24
PfoI T'CCnGG_A
Cuts at: 0 116 488 780
Size: 116 372 292
Fragments arranged by size:
372 292 116
PpuMI rG'GwC_Cy
Cuts at: 0 95 275 486 780
Size: 95 180 211 294
Fragments arranged by size:
294 211 180 95
PspOMI G'GGCC_C
Cuts at: 0 101 161 598 780
Size: 101 60 437 182
Fragments arranged by size:
437 182 101 60
PstI C_TGCA'G
Cuts at: 0 139 458 780
Size: 139 319 322
Fragments arranged by size:
322 319 139
PvuII CAG'CTG
Cuts at: 0 609 780
Size: 609 171
SacII CC_GC'GG
Cuts at: 0 8 780
Size: 8 772
SanDI GG'GwC_CC
Cuts at: 0 275 780
Size: 275 505
SapI GCTCTTCn'nnn_
Cuts at: 0 640 780
Size: 640 140
SbfI CC_TGCA'GG
Cuts at: 0 139 780
Size: 139 641
SfcI C'TryA_G
Cuts at: 0 135 454 780
Size: 135 319 326
Fragments arranged by size:
326 319 135
SfoI GGC'GCC
Cuts at: 0 37 230 293 407 780
Size: 37 193 63 114 373
Fragments arranged by size:
373 193 114 63 37
StuI AGG'CCT
Cuts at: 0 133 780
Size: 133 647
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 484 507 780
Size: 484 23 273
Fragments arranged by size:
484 273 23
TatI w'GTAC_w
Cuts at: 0 647 780
Size: 647 133
Enzymes that do cut:
AarI AlwNI ApaI ApoI AscI AvaI BanI BanII
BbeI BglI Bme1580I BmgBI BpmI Bpu10I BsaI BsaHI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI
BsrBI BsrFI BssHII BstAPI BtgI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI HaeII HincII KasI
MspA1I NaeI NarI NgoMIV PflMI PfoI PpuMI PspOMI
PstI PvuII SacII SanDI SapI SbfI SfcI SfoI
StuI TaqII TatI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmrI BmtI BplI BpuEI BsaAI
BsaBI BsaWI BsaXI BsiWI BsmBI BspEI BspHI BsrDI
BsrGI BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EcoICRI
EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SalI ScaI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StyI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI