Restriction map for
pFN21AE2622
(Linear) (Six-base) MAPSORT of: KIEE2622.seq Check: 5973 from: 1 to: 1047
KIEE2622.orf
With 182 enzymes: SgfI *
May 6, 2016 19:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 657 716 1047
Size: 657 59 331
Fragments arranged by size:
657 331 59
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 868 900 1047
Size: 868 32 147
Fragments arranged by size:
868 147 32
AlwNI CAG_nnn'CTG
Cuts at: 0 277 916 955 1047
Size: 277 639 39 92
Fragments arranged by size:
639 277 92 39
ApaI G_GGCC'C
Cuts at: 0 8 1047
Size: 8 1039
BamHI G'GATC_C
Cuts at: 0 352 883 1047
Size: 352 531 164
Fragments arranged by size:
531 352 164
BanII G_rGCy'C
Cuts at: 0 8 53 1047
Size: 8 45 994
Fragments arranged by size:
994 45 8
BclI T'GATC_A
Cuts at: 0 944 1047
Size: 944 103
BfrBI ATG'CAT
Cuts at: 0 313 1047
Size: 313 734
BglI GCCn_nnn'nGGC
Cuts at: 0 1036 1047
Size: 1036 11
BlpI GC'TnA_GC
Cuts at: 0 101 1047
Size: 101 946
Bme1580I G_kGCm'C
Cuts at: 0 8 1047
Size: 8 1039
BmrI ACTGGGnnnn_n'
Cuts at: 0 676 1047
Size: 676 371
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 1047
Size: 432 615
BsaWI w'CCGG_w
Cuts at: 0 423 1047
Size: 423 624
BseYI C'CCAG_C
Cuts at: 0 657 1047
Size: 657 390
Bsp1286I G_dGCh'C
Cuts at: 0 8 53 1047
Size: 8 45 994
Fragments arranged by size:
994 45 8
BspEI T'CCGG_A
Cuts at: 0 423 1047
Size: 423 624
BspHI T'CATG_A
Cuts at: 0 1013 1047
Size: 1013 34
BssSI C'ACGA_G
Cuts at: 0 185 1047
Size: 185 862
BstXI CCAn_nnnn'nTGG
Cuts at: 0 468 1047
Size: 468 579
BstYI r'GATC_y
Cuts at: 0 352 883 1047
Size: 352 531 164
Fragments arranged by size:
531 352 164
BtgI C'CryG_G
Cuts at: 0 467 1047
Size: 467 580
BtsI GCAGTG_nn'
Cuts at: 0 95 1047
Size: 95 952
EaeI y'GGCC_r
Cuts at: 0 405 918 1028 1047
Size: 405 513 110 19
Fragments arranged by size:
513 405 110 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 657 716 1047
Size: 432 225 59 331
Fragments arranged by size:
432 331 225 59
EcoO109I rG'GnC_Cy
Cuts at: 0 4 180 274 593 631 1047
Size: 4 176 94 319 38 416
Fragments arranged by size:
416 319 176 94 38 4
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 20 52 337 369 585 617 989 1021
Size: 20 32 285 32 216 32 372 32
Cuts at: 1021 1047
Size: 26
Fragments arranged by size:
372 285 216 32 32 32 32 26
20
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 1047
Size: 485 562
MscI TGG'CCA
Cuts at: 0 407 1030 1047
Size: 407 623 17
Fragments arranged by size:
623 407 17
MspA1I CmG'CkG
Cuts at: 0 576 881 1047
Size: 576 305 166
Fragments arranged by size:
576 305 166
NsiI A_TGCA'T
Cuts at: 0 315 1047
Size: 315 732
PasI CC'CwG_GG
Cuts at: 0 271 1047
Size: 271 776
PpuMI rG'GwC_Cy
Cuts at: 0 180 593 631 1047
Size: 180 413 38 416
Fragments arranged by size:
416 413 180 38
PshAI GACnn'nnGTC
Cuts at: 0 934 1047
Size: 934 113
PspOMI G'GGCC_C
Cuts at: 0 4 1047
Size: 4 1043
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 508 540 1047
Size: 508 32 507
Fragments arranged by size:
508 507 32
PstI C_TGCA'G
Cuts at: 0 28 138 575 1047
Size: 28 110 437 472
Fragments arranged by size:
472 437 110 28
PvuII CAG'CTG
Cuts at: 0 576 1047
Size: 576 471
ScaI AGT'ACT
Cuts at: 0 1025 1047
Size: 1025 22
SfcI C'TryA_G
Cuts at: 0 24 134 571 1047
Size: 24 110 437 476
Fragments arranged by size:
476 437 110 24
TatI w'GTAC_w
Cuts at: 0 966 1023 1047
Size: 966 57 24
Fragments arranged by size:
966 57 24
Tth111I GACn'n_nGTC
Cuts at: 0 682 1047
Size: 682 365
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 184 1047
Size: 184 863
Enzymes that do cut:
AcuI AloI AlwNI ApaI BamHI BanII BclI BfrBI
BglI BlpI Bme1580I BmrI BpmI BsaWI BseYI Bsp1286I
BspEI BspHI BssSI BstXI BstYI BtgI BtsI EaeI
Eco57MI EcoO109I Hin4I MmeI MscI MspA1I NsiI PasI
PpuMI PshAI PspOMI PsrI PstI PvuII ScaI SfcI
TatI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BbsI BbvCI
BcgI BciVI BglII BmgBI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaXI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EarI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PacI PciI PflMI PfoI PmeI PmlI
PpiI PsiI PspXI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TaqII TstI XbaI XhoI XmaI XmnI
ZraI