Restriction map for
pFN21AE2620
(Linear) (Six-base) MAPSORT of: KIEE2620.seq Check: 8709 from: 1 to: 1644
KIEE2620.orf
With 182 enzymes: SgfI *
May 6, 2016 19:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 388 793 1644
Size: 388 405 851
Fragments arranged by size:
851 405 388
AatII G_ACGT'C
Cuts at: 0 626 1644
Size: 626 1018
AflIII A'CryG_T
Cuts at: 0 203 1644
Size: 203 1441
AleI CACnn'nnGTG
Cuts at: 0 1327 1644
Size: 1327 317
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 493 1067 1644
Size: 493 574 577
Fragments arranged by size:
577 574 493
ApaI G_GGCC'C
Cuts at: 0 925 1099 1644
Size: 925 174 545
Fragments arranged by size:
925 545 174
AvaI C'yCGr_G
Cuts at: 0 51 87 501 1342 1375 1644
Size: 51 36 414 841 33 269
Fragments arranged by size:
841 414 269 51 36 33
BanI G'GyrC_C
Cuts at: 0 1420 1644
Size: 1420 224
BanII G_rGCy'C
Cuts at: 0 307 371 925 1099 1343 1644
Size: 307 64 554 174 244 301
Fragments arranged by size:
554 307 301 244 174 64
BbsI GAAGACnn'nnnn_
Cuts at: 0 773 1644
Size: 773 871
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1284 1318 1644
Size: 1284 34 326
Fragments arranged by size:
1284 326 34
BclI T'GATC_A
Cuts at: 0 686 699 1644
Size: 686 13 945
Fragments arranged by size:
945 686 13
BglI GCCn_nnn'nGGC
Cuts at: 0 89 443 1369 1644
Size: 89 354 926 275
Fragments arranged by size:
926 354 275 89
BglII A'GATC_T
Cuts at: 0 1589 1644
Size: 1589 55
BlpI GC'TnA_GC
Cuts at: 0 109 481 1282 1644
Size: 109 372 801 362
Fragments arranged by size:
801 372 362 109
Bme1580I G_kGCm'C
Cuts at: 0 100 295 467 925 1056 1099 1423 1644
Size: 100 195 172 458 131 43 324 221
Fragments arranged by size:
458 324 221 195 172 131 100 43
BmgBI CAC'GTC
Cuts at: 0 1383 1644
Size: 1383 261
BmrI ACTGGGnnnn_n'
Cuts at: 0 1467 1644
Size: 1467 177
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1354 1453 1624 1644
Size: 1354 99 171 20
Fragments arranged by size:
1354 171 99 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1372 1393 1644
Size: 1372 21 251
Fragments arranged by size:
1372 251 21
BsaI GGTCTCn'nnnn_
Cuts at: 0 508 1018 1644
Size: 508 510 626
Fragments arranged by size:
626 510 508
BsaAI yAC'GTr
Cuts at: 0 477 1644
Size: 477 1167
BsaHI Gr'CG_yC
Cuts at: 0 131 425 623 770 1070 1544 1644
Size: 131 294 198 147 300 474 100
Fragments arranged by size:
474 300 294 198 147 131 100
BsaWI w'CCGG_w
Cuts at: 0 1265 1644
Size: 1265 379
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2 32 1644
Size: 2 30 1612
Fragments arranged by size:
1612 30 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 28 1351 1644
Size: 25 3 1323 293
Fragments arranged by size:
1323 293 25 3
BseYI C'CCAG_C
Cuts at: 0 75 249 370 493 805 871 879 1109
Size: 75 174 121 123 312 66 8 230
Cuts at: 1109 1121 1644
Size: 12 523
Fragments arranged by size:
523 312 230 174 123 121 75 66
12 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 433 610 771 900 928 1111 1644
Size: 199 234 177 161 129 28 183 533
Fragments arranged by size:
533 234 199 183 177 161 129 28
BsiEI CG_ry'CG
Cuts at: 0 354 633 890 1644
Size: 354 279 257 754
Fragments arranged by size:
754 354 279 257
BsiHKAI G_wGCw'C
Cuts at: 0 142 482 749 1388 1644
Size: 142 340 267 639 256
Fragments arranged by size:
639 340 267 256 142
Bsp1286I G_dGCh'C
Cuts at: 0 100 142 295 307 371 467 482 749
Size: 100 42 153 12 64 96 15 267
Cuts at: 749 925 1056 1099 1343 1388 1423 1644
Size: 176 131 43 244 45 35 221
Fragments arranged by size:
267 244 221 176 153 131 100 96
64 45 43 42 35 15 12
BspEI T'CCGG_A
Cuts at: 0 1265 1644
Size: 1265 379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 388 582 793 1644
Size: 388 194 211 851
Fragments arranged by size:
851 388 211 194
BsrFI r'CCGG_y
Cuts at: 0 772 852 1351 1546 1644
Size: 772 80 499 195 98
Fragments arranged by size:
772 499 195 98 80
BsrGI T'GTAC_A
Cuts at: 0 563 779 1644
Size: 563 216 865
Fragments arranged by size:
865 563 216
BssHII G'CGCG_C
Cuts at: 0 793 1644
Size: 793 851
BstAPI GCAn_nnn'nTGC
Cuts at: 0 744 1644
Size: 744 900
BstEII G'GTnAC_C
Cuts at: 0 723 1644
Size: 723 921
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1431 1644
Size: 1431 213
BstYI r'GATC_y
Cuts at: 0 1589 1644
Size: 1589 55
BtgI C'CryG_G
Cuts at: 0 993 1014 1056 1644
Size: 993 21 42 588
Fragments arranged by size:
993 588 42 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1344 1538 1644
Size: 1344 194 106
Fragments arranged by size:
1344 194 106
BtsI GCAGTG_nn'
Cuts at: 0 518 615 1153 1644
Size: 518 97 538 491
Fragments arranged by size:
538 518 491 97
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 189 224 1644
Size: 189 35 1420
Fragments arranged by size:
1420 189 35
DraIII CAC_nnn'GTG
Cuts at: 0 462 1644
Size: 462 1182
EaeI y'GGCC_r
Cuts at: 0 487 605 630 854 887 1532 1644
Size: 487 118 25 224 33 645 112
Fragments arranged by size:
645 487 224 118 112 33 25
EagI C'GGCC_G
Cuts at: 0 630 887 1644
Size: 630 257 757
Fragments arranged by size:
757 630 257
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1354 1453 1624 1644
Size: 1354 99 171 20
Fragments arranged by size:
1354 171 99 20
EcoO109I rG'GnC_Cy
Cuts at: 0 444 922 1095 1096 1644
Size: 444 478 173 1 548
Fragments arranged by size:
548 478 444 173 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1608 1644
Size: 1608 36
HaeII r_GCGC'y
Cuts at: 0 1282 1375 1644
Size: 1282 93 269
Fragments arranged by size:
1282 269 93
MslI CAynn'nnrTG
Cuts at: 0 202 1327 1644
Size: 202 1125 317
Fragments arranged by size:
1125 317 202
MspA1I CmG'CkG
Cuts at: 0 221 253 268 344 356 413 485 629
Size: 221 32 15 76 12 57 72 144
Cuts at: 629 741 966 1016 1058 1137 1500 1644
Size: 112 225 50 42 79 363 144
Fragments arranged by size:
363 225 221 144 144 112 79 76
72 57 50 42 32 15 12
NaeI GCC'GGC
Cuts at: 0 774 854 1353 1548 1644
Size: 774 80 499 195 96
Fragments arranged by size:
774 499 195 96 80
NgoMIV G'CCGG_C
Cuts at: 0 772 852 1351 1546 1644
Size: 772 80 499 195 98
Fragments arranged by size:
772 499 195 98 80
NotI GC'GGCC_GC
Cuts at: 0 630 1644
Size: 630 1014
NspI r_CATG'y
Cuts at: 0 1040 1644
Size: 1040 604
PflMI CCAn_nnn'nTGG
Cuts at: 0 122 1644
Size: 122 1522
PfoI T'CCnGG_A
Cuts at: 0 1637 1644
Size: 1637 7
PmlI CAC'GTG
Cuts at: 0 477 1644
Size: 477 1167
PspOMI G'GGCC_C
Cuts at: 0 921 1095 1644
Size: 921 174 549
Fragments arranged by size:
921 549 174
PspXI vC'TCGA_Gb
Cuts at: 0 1375 1644
Size: 1375 269
PstI C_TGCA'G
Cuts at: 0 1145 1644
Size: 1145 499
PvuII CAG'CTG
Cuts at: 0 221 253 485 741 1644
Size: 221 32 232 256 903
Fragments arranged by size:
903 256 232 221 32
SacII CC_GC'GG
Cuts at: 0 1017 1059 1644
Size: 1017 42 585
Fragments arranged by size:
1017 585 42
SfcI C'TryA_G
Cuts at: 0 1128 1141 1644
Size: 1128 13 503
Fragments arranged by size:
1128 503 13
SgrAI Cr'CCGG_yG
Cuts at: 0 1351 1644
Size: 1351 293
SmaI CCC'GGG
Cuts at: 0 89 503 1644
Size: 89 414 1141
Fragments arranged by size:
1141 414 89
SmlI C'TyrA_G
Cuts at: 0 1375 1387 1408 1644
Size: 1375 12 21 236
Fragments arranged by size:
1375 236 21 12
SphI G_CATG'C
Cuts at: 0 1040 1644
Size: 1040 604
StuI AGG'CCT
Cuts at: 0 433 913 1048 1615 1644
Size: 433 480 135 567 29
Fragments arranged by size:
567 480 433 135 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 114 1501 1644
Size: 114 1387 143
Fragments arranged by size:
1387 143 114
TatI w'GTAC_w
Cuts at: 0 563 779 1644
Size: 563 216 865
Fragments arranged by size:
865 563 216
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 926 958 1644
Size: 926 32 686
Fragments arranged by size:
926 686 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 123 1644
Size: 123 1521
XhoI C'TCGA_G
Cuts at: 0 1375 1644
Size: 1375 269
XmaI C'CCGG_G
Cuts at: 0 87 501 1644
Size: 87 414 1143
Fragments arranged by size:
1143 414 87
ZraI GAC'GTC
Cuts at: 0 624 1644
Size: 624 1020
Enzymes that do cut:
AarI AatII AflIII AleI AlfI ApaI AvaI BanI
BanII BbsI BcgI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BpmI BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspMI BsrFI BsrGI BssHII BstAPI BstEII BstXI BstYI
BtgI BtgZI BtsI CspCI DraIII EaeI EagI Eco57MI
EcoO109I FalI HaeII MslI MspA1I NaeI NgoMIV NotI
NspI PflMI PfoI PmlI PspOMI PspXI PstI PvuII
SacII SfcI SgrAI SmaI SmlI SphI StuI TaqII
TatI TstI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI
AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BciVI BfrBI BmtI BplI
Bpu10I BsaBI BsiWI BsmI BsmBI BspHI BsrBI BsrDI
BssSI BstBI BstZ17I Bsu36I ClaI DraI DrdI EarI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NarI NcoI NdeI NheI NruI NsiI
PacI PasI PciI PmeI PpiI PpuMI PshAI PsiI
PsrI PvuI RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SnaBI SpeI SrfI
SspI StyI SwaI Tth111I XbaI XmnI