Restriction map for
pFN21AE2619
(Linear) (Six-base) MAPSORT of: KIEE2619.seq Check: 47 from: 1 to: 1368
KIEE2619.orf
With 182 enzymes: SgfI *
April 15, 2016 10:56 ..
AflIII A'CryG_T
Cuts at: 0 129 1248 1368
Size: 129 1119 120
Fragments arranged by size:
1119 129 120
ApoI r'AATT_y
Cuts at: 0 312 384 1069 1368
Size: 312 72 685 299
Fragments arranged by size:
685 312 299 72
AseI AT'TA_AT
Cuts at: 0 469 1368
Size: 469 899
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 290 323 1368
Size: 290 33 1045
Fragments arranged by size:
1045 290 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 451 962 1368
Size: 451 511 406
Fragments arranged by size:
511 451 406
BclI T'GATC_A
Cuts at: 0 90 1368
Size: 90 1278
BglII A'GATC_T
Cuts at: 0 277 781 1368
Size: 277 504 587
Fragments arranged by size:
587 504 277
BlpI GC'TnA_GC
Cuts at: 0 652 1368
Size: 652 716
BmgBI CAC'GTC
Cuts at: 0 1028 1368
Size: 1028 340
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1350 1368
Size: 1350 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 798 1368
Size: 798 570
Bpu10I CC'TnA_GC
Cuts at: 0 614 1368
Size: 614 754
BsaAI yAC'GTr
Cuts at: 0 130 1251 1368
Size: 130 1121 117
Fragments arranged by size:
1121 130 117
BsaBI GATnn'nnATC
Cuts at: 0 153 683 1368
Size: 153 530 685
Fragments arranged by size:
685 530 153
BsiEI CG_ry'CG
Cuts at: 0 1131 1368
Size: 1131 237
BsiHKAI G_wGCw'C
Cuts at: 0 870 1368
Size: 870 498
Bsp1286I G_dGCh'C
Cuts at: 0 870 1368
Size: 870 498
BspHI T'CATG_A
Cuts at: 0 675 1368
Size: 675 693
BstYI r'GATC_y
Cuts at: 0 277 781 1368
Size: 277 504 587
Fragments arranged by size:
587 504 277
Bsu36I CC'TnA_GG
Cuts at: 0 1349 1368
Size: 1349 19
BtsI GCAGTG_nn'
Cuts at: 0 794 1051 1308 1368
Size: 794 257 257 60
Fragments arranged by size:
794 257 257 60
EarI CTCTTCn'nnn_
Cuts at: 0 159 1368
Size: 159 1209
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 798 1368
Size: 798 570
EcoNI CCTnn'n_nnAGG
Cuts at: 0 57 1368
Size: 57 1311
EcoO109I rG'GnC_Cy
Cuts at: 0 923 1368
Size: 923 445
EcoRI G'AATT_C
Cuts at: 0 1069 1368
Size: 1069 299
EcoRV GAT'ATC
Cuts at: 0 271 393 645 1368
Size: 271 122 252 723
Fragments arranged by size:
723 271 252 122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 6 38 852 884 895 927 1368
Size: 6 32 814 32 11 32 441
Fragments arranged by size:
814 441 32 32 32 11 6
HindIII A'AGCT_T
Cuts at: 0 435 616 1368
Size: 435 181 752
Fragments arranged by size:
752 435 181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 1003 1368
Size: 354 649 365
Fragments arranged by size:
649 365 354
MslI CAynn'nnrTG
Cuts at: 0 112 692 1109 1368
Size: 112 580 417 259
Fragments arranged by size:
580 417 259 112
MspA1I CmG'CkG
Cuts at: 0 667 1271 1368
Size: 667 604 97
Fragments arranged by size:
667 604 97
PflMI CCAn_nnn'nTGG
Cuts at: 0 732 1368
Size: 732 636
PmlI CAC'GTG
Cuts at: 0 1251 1368
Size: 1251 117
PpuMI rG'GwC_Cy
Cuts at: 0 923 1368
Size: 923 445
PshAI GACnn'nnGTC
Cuts at: 0 890 1368
Size: 890 478
PsiI TTA'TAA
Cuts at: 0 341 1368
Size: 341 1027
PstI C_TGCA'G
Cuts at: 0 789 1286 1368
Size: 789 497 82
Fragments arranged by size:
789 497 82
PvuI CG_AT'CG
Cuts at: 0 1131 1368
Size: 1131 237
PvuII CAG'CTG
Cuts at: 0 667 1271 1368
Size: 667 604 97
Fragments arranged by size:
667 604 97
ScaI AGT'ACT
Cuts at: 0 478 761 1368
Size: 478 283 607
Fragments arranged by size:
607 478 283
SfcI C'TryA_G
Cuts at: 0 785 1282 1368
Size: 785 497 86
Fragments arranged by size:
785 497 86
StyI C'CwwG_G
Cuts at: 0 29 637 1368
Size: 29 608 731
Fragments arranged by size:
731 608 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 191 1099 1368
Size: 191 908 269
Fragments arranged by size:
908 269 191
TatI w'GTAC_w
Cuts at: 0 476 759 1368
Size: 476 283 609
Fragments arranged by size:
609 476 283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 360 678 1025 1240 1368
Size: 360 318 347 215 128
Fragments arranged by size:
360 347 318 215 128
XmnI GAAnn'nnTTC
Cuts at: 0 168 1368
Size: 168 1200
Enzymes that do cut:
AflIII ApoI AseI BaeI BbsI BclI BglII BlpI
BmgBI BplI BpmI Bpu10I BsaAI BsaBI BsiEI BsiHKAI
Bsp1286I BspHI BstYI Bsu36I BtsI EarI Eco57MI EcoNI
EcoO109I EcoRI EcoRV Hin4I HindIII MmeI MslI MspA1I
PflMI PmlI PpuMI PshAI PsiI PstI PvuI PvuII
ScaI SfcI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbvCI BcgI BciVI BfrBI BglI Bme1580I BmrI BmtI
BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
BtgI BtgZI ClaI CspCI DraI DraIII DrdI EaeI
EagI EciI EcoICRI FalI FseI FspI FspAI HaeII
HincII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PpiI PspOMI
PspXI PsrI RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XhoI XmaI ZraI