Restriction map for
pFN21AE2616
(Linear) (Six-base) MAPSORT of: KIEE2616.seq Check: 2524 from: 1 to: 1350
KIEE2616.orf
With 182 enzymes: SgfI *
April 1, 2016 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 690 1350
Size: 690 660
AatII G_ACGT'C
Cuts at: 0 422 1350
Size: 422 928
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 907 1350
Size: 907 443
AflIII A'CryG_T
Cuts at: 0 911 1350
Size: 911 439
AhdI GACnn_n'nnGTC
Cuts at: 0 177 1350
Size: 177 1173
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 790 822 1350
Size: 790 32 528
Fragments arranged by size:
790 528 32
AlwNI CAG_nnn'CTG
Cuts at: 0 370 635 705 795 1005 1340 1350
Size: 370 265 70 90 210 335 10
Fragments arranged by size:
370 335 265 210 90 70 10
ApaI G_GGCC'C
Cuts at: 0 1193 1201 1349 1350
Size: 1193 8 148 1
Fragments arranged by size:
1193 148 8 1
ApaLI G'TGCA_C
Cuts at: 0 1102 1350
Size: 1102 248
ApoI r'AATT_y
Cuts at: 0 595 1350
Size: 595 755
AvaI C'yCGr_G
Cuts at: 0 448 1201 1281 1350
Size: 448 753 80 69
Fragments arranged by size:
753 448 80 69
BanI G'GyrC_C
Cuts at: 0 26 186 564 874 993 1350
Size: 26 160 378 310 119 357
Fragments arranged by size:
378 357 310 160 119 26
BanII G_rGCy'C
Cuts at: 0 449 808 853 1193 1201 1349 1350
Size: 449 359 45 340 8 148 1
Fragments arranged by size:
449 359 340 148 45 8 1
BbeI G_GCGC'C
Cuts at: 0 190 1350
Size: 190 1160
BglI GCCn_nnn'nGGC
Cuts at: 0 379 1350
Size: 379 971
BlpI GC'TnA_GC
Cuts at: 0 55 1350
Size: 55 1295
Bme1580I G_kGCm'C
Cuts at: 0 134 569 1106 1193 1201 1349 1350
Size: 134 435 537 87 8 148 1
Fragments arranged by size:
537 435 148 134 87 8 1
BmrI ACTGGGnnnn_n'
Cuts at: 0 36 1263 1350
Size: 36 1227 87
Fragments arranged by size:
1227 87 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 703 997 1350
Size: 464 239 294 353
Fragments arranged by size:
464 353 294 239
BsaI GGTCTCn'nnnn_
Cuts at: 0 1284 1350
Size: 1284 66
BsaAI yAC'GTr
Cuts at: 0 411 914 1350
Size: 411 503 436
Fragments arranged by size:
503 436 411
BsaHI Gr'CG_yC
Cuts at: 0 187 419 1350
Size: 187 232 931
Fragments arranged by size:
931 232 187
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 790 820 1350
Size: 790 30 530
Fragments arranged by size:
790 530 30
BseYI C'CCAG_C
Cuts at: 0 157 705 1324 1350
Size: 157 548 619 26
Fragments arranged by size:
619 548 157 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1278 1350
Size: 1278 72
BsiEI CG_ry'CG
Cuts at: 0 756 1350
Size: 756 594
BsiHKAI G_wGCw'C
Cuts at: 0 119 410 449 808 1106 1350
Size: 119 291 39 359 298 244
Fragments arranged by size:
359 298 291 244 119 39
BsmBI CGTCTCn'nnnn_
Cuts at: 0 896 1350
Size: 896 454
Bsp1286I G_dGCh'C
Cuts at: 0 119 134 410 449 569 808 853 1106
Size: 119 15 276 39 120 239 45 253
Cuts at: 1106 1193 1201 1349 1350
Size: 87 8 148 1
Fragments arranged by size:
276 253 239 148 120 119 87 45
39 15 8 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 690 1350
Size: 690 660
BsrBI CCG'CTC
Cuts at: 0 291 1350
Size: 291 1059
BsrFI r'CCGG_y
Cuts at: 0 183 544 653 713 877 1077 1350
Size: 183 361 109 60 164 200 273
Fragments arranged by size:
361 273 200 183 164 109 60
BsrGI T'GTAC_A
Cuts at: 0 611 1350
Size: 611 739
BssSI C'ACGA_G
Cuts at: 0 442 1350
Size: 442 908
BstEII G'GTnAC_C
Cuts at: 0 13 1350
Size: 13 1337
BstYI r'GATC_y
Cuts at: 0 950 1350
Size: 950 400
BtgI C'CryG_G
Cuts at: 0 35 65 1193 1218 1274 1350
Size: 35 30 1128 25 56 76
Fragments arranged by size:
1128 76 56 35 30 25
BtsI GCAGTG_nn'
Cuts at: 0 368 1235 1290 1350
Size: 368 867 55 60
Fragments arranged by size:
867 368 60 55
DraIII CAC_nnn'GTG
Cuts at: 0 1230 1350
Size: 1230 120
EaeI y'GGCC_r
Cuts at: 0 95 260 747 1221 1350
Size: 95 165 487 474 129
Fragments arranged by size:
487 474 165 129 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 798 1289 1350
Size: 798 491 61
Fragments arranged by size:
798 491 61
EcoICRI GAG'CTC
Cuts at: 0 447 806 1350
Size: 447 359 544
Fragments arranged by size:
544 447 359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 703 907 997 1350
Size: 464 239 204 90 353
Fragments arranged by size:
464 353 239 204 90
EcoO109I rG'GnC_Cy
Cuts at: 0 792 1189 1198 1286 1337 1345 1350
Size: 792 397 9 88 51 8 5
Fragments arranged by size:
792 397 88 51 9 8 5
EcoRI G'AATT_C
Cuts at: 0 595 1350
Size: 595 755
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 855 1350
Size: 855 495
HaeII r_GCGC'y
Cuts at: 0 190 1268 1350
Size: 190 1078 82
Fragments arranged by size:
1078 190 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 163 195 1035 1067 1350
Size: 163 32 840 32 283
Fragments arranged by size:
840 283 163 32 32
HincII GTy'rAC
Cuts at: 0 1134 1350
Size: 1134 216
KasI G'GCGC_C
Cuts at: 0 186 1350
Size: 186 1164
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 1350
Size: 227 1123
MscI TGG'CCA
Cuts at: 0 1223 1350
Size: 1223 127
MslI CAynn'nnrTG
Cuts at: 0 499 1350
Size: 499 851
MspA1I CmG'CkG
Cuts at: 0 635 1140 1350
Size: 635 505 210
Fragments arranged by size:
635 505 210
NaeI GCC'GGC
Cuts at: 0 185 1350
Size: 185 1165
NarI GG'CG_CC
Cuts at: 0 187 1350
Size: 187 1163
NcoI C'CATG_G
Cuts at: 0 65 1218 1274 1350
Size: 65 1153 56 76
Fragments arranged by size:
1153 76 65 56
NgoMIV G'CCGG_C
Cuts at: 0 183 1350
Size: 183 1167
NspI r_CATG'y
Cuts at: 0 329 1350
Size: 329 1021
PflMI CCAn_nnn'nTGG
Cuts at: 0 1143 1275 1350
Size: 1143 132 75
Fragments arranged by size:
1143 132 75
PmlI CAC'GTG
Cuts at: 0 411 1350
Size: 411 939
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 790 822 1350
Size: 790 32 528
Fragments arranged by size:
790 528 32
PpuMI rG'GwC_Cy
Cuts at: 0 1286 1337 1350
Size: 1286 51 13
Fragments arranged by size:
1286 51 13
PshAI GACnn'nnGTC
Cuts at: 0 418 1350
Size: 418 932
PspOMI G'GGCC_C
Cuts at: 0 1189 1197 1345 1350
Size: 1189 8 148 5
Fragments arranged by size:
1189 148 8 5
PvuII CAG'CTG
Cuts at: 0 635 1140 1350
Size: 635 505 210
Fragments arranged by size:
635 505 210
SacI G_AGCT'C
Cuts at: 0 449 808 1350
Size: 449 359 542
Fragments arranged by size:
542 449 359
SanDI GG'GwC_CC
Cuts at: 0 1286 1350
Size: 1286 64
SfcI C'TryA_G
Cuts at: 0 1039 1057 1350
Size: 1039 18 293
Fragments arranged by size:
1039 293 18
SfoI GGC'GCC
Cuts at: 0 188 1350
Size: 188 1162
SgrAI Cr'CCGG_yG
Cuts at: 0 183 1350
Size: 183 1167
SmaI CCC'GGG
Cuts at: 0 1203 1350
Size: 1203 147
StyI C'CwwG_G
Cuts at: 0 65 870 1218 1274 1350
Size: 65 805 348 56 76
Fragments arranged by size:
805 348 76 65 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 151 430 770 1194 1350
Size: 151 279 340 424 156
Fragments arranged by size:
424 340 279 156 151
TatI w'GTAC_w
Cuts at: 0 611 1350
Size: 611 739
Tth111I GACn'n_nGTC
Cuts at: 0 538 1350
Size: 538 812
XmaI C'CCGG_G
Cuts at: 0 1201 1350
Size: 1201 149
ZraI GAC'GTC
Cuts at: 0 420 1350
Size: 420 930
Enzymes that do cut:
AarI AatII AcuI AflIII AhdI AloI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbeI BglI BlpI
Bme1580I BmrI BpmI BsaI BsaAI BsaHI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI
BsrGI BssSI BstEII BstYI BtgI BtsI DraIII EaeI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I
HincII KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PflMI PmlI PpiI PpuMI PshAI
PspOMI PvuII SacI SanDI SfcI SfoI SgrAI SmaI
StyI TaqII TatI Tth111I XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AleI AlfI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BmgBI BmtI BplI
Bpu10I BpuEI BsaBI BsaWI BseRI BsiWI BsmI BspEI
BspHI BsrDI BssHII BstAPI BstBI BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DrdI EagI EarI EcoNI
EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI NdeI NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PsiI PspXI PsrI PstI PvuI
RsrII SacII SalI SapI SbfI ScaI SexAI SfiI
SgfI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI XbaI XcmI XhoI XmnI