Restriction map for
pFN21AE2612
(Linear) (Six-base) MAPSORT of: KIEE2612.seq Check: 3678 from: 1 to: 1155
KIEE2612.orf
With 182 enzymes: SgfI *
March 9, 2016 16:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 90 882 1155
Size: 90 792 273
Fragments arranged by size:
792 273 90
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 936 1155
Size: 706 230 219
Fragments arranged by size:
706 230 219
AfeI AGC'GCT
Cuts at: 0 402 1155
Size: 402 753
AflIII A'CryG_T
Cuts at: 0 74 659 1155
Size: 74 585 496
Fragments arranged by size:
585 496 74
AgeI A'CCGG_T
Cuts at: 0 707 1155
Size: 707 448
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 551 583 1155
Size: 551 32 572
Fragments arranged by size:
572 551 32
ApoI r'AATT_y
Cuts at: 0 715 1155
Size: 715 440
AvaI C'yCGr_G
Cuts at: 0 58 476 1155
Size: 58 418 679
Fragments arranged by size:
679 418 58
AvrII C'CTAG_G
Cuts at: 0 502 1155
Size: 502 653
BanI G'GyrC_C
Cuts at: 0 1021 1155
Size: 1021 134
BanII G_rGCy'C
Cuts at: 0 317 950 1155
Size: 317 633 205
Fragments arranged by size:
633 317 205
BbsI GAAGACnn'nnnn_
Cuts at: 0 685 1036 1155
Size: 685 351 119
Fragments arranged by size:
685 351 119
BlpI GC'TnA_GC
Cuts at: 0 1066 1155
Size: 1066 89
BmrI ACTGGGnnnn_n'
Cuts at: 0 1088 1155
Size: 1088 67
BmtI G_CTAG'C
Cuts at: 0 791 1155
Size: 791 364
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 385 417 1155
Size: 331 54 32 738
Fragments arranged by size:
738 331 54 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 871 1155
Size: 212 659 284
Fragments arranged by size:
659 284 212
BsaWI w'CCGG_w
Cuts at: 0 707 739 864 1155
Size: 707 32 125 291
Fragments arranged by size:
707 291 125 32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 736 766 1155
Size: 736 30 389
Fragments arranged by size:
736 389 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 76 726 1155
Size: 76 650 429
Fragments arranged by size:
650 429 76
BseYI C'CCAG_C
Cuts at: 0 390 460 814 1155
Size: 390 70 354 341
Fragments arranged by size:
390 354 341 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 1155
Size: 118 1037
BsiHKAI G_wGCw'C
Cuts at: 0 950 1155
Size: 950 205
BsmI GAATG_Cn'
Cuts at: 0 1020 1155
Size: 1020 135
Bsp1286I G_dGCh'C
Cuts at: 0 317 950 1155
Size: 317 633 205
Fragments arranged by size:
633 317 205
BspEI T'CCGG_A
Cuts at: 0 739 1155
Size: 739 416
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 90 882 1155
Size: 90 792 273
Fragments arranged by size:
792 273 90
BsrBI CCG'CTC
Cuts at: 0 549 1155
Size: 549 606
BsrDI GCAATG_nn'
Cuts at: 0 928 1155
Size: 928 227
BsrFI r'CCGG_y
Cuts at: 0 707 980 1155
Size: 707 273 175
Fragments arranged by size:
707 273 175
BssHII G'CGCG_C
Cuts at: 0 117 1155
Size: 117 1038
BssSI C'ACGA_G
Cuts at: 0 7 94 1155
Size: 7 87 1061
Fragments arranged by size:
1061 87 7
BstEII G'GTnAC_C
Cuts at: 0 45 1155
Size: 45 1110
BstXI CCAn_nnnn'nTGG
Cuts at: 0 309 1078 1155
Size: 309 769 77
Fragments arranged by size:
769 309 77
Bsu36I CC'TnA_GG
Cuts at: 0 68 1155
Size: 68 1087
BtgI C'CryG_G
Cuts at: 0 155 914 1155
Size: 155 759 241
Fragments arranged by size:
759 241 155
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 956 1155
Size: 956 199
BtsI GCAGTG_nn'
Cuts at: 0 352 680 1155
Size: 352 328 475
Fragments arranged by size:
475 352 328
EaeI y'GGCC_r
Cuts at: 0 335 620 1155
Size: 335 285 535
Fragments arranged by size:
535 335 285
EarI CTCTTCn'nnn_
Cuts at: 0 955 1142 1155
Size: 955 187 13
Fragments arranged by size:
955 187 13
EcoICRI GAG'CTC
Cuts at: 0 948 1155
Size: 948 207
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 385 417 706 936 1155
Size: 331 54 32 289 230 219
Fragments arranged by size:
331 289 230 219 54 32
EcoNI CCTnn'n_nnAGG
Cuts at: 0 97 760 1155
Size: 97 663 395
Fragments arranged by size:
663 395 97
EcoO109I rG'GnC_Cy
Cuts at: 0 765 1155
Size: 765 390
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 784 1155
Size: 784 371
HaeII r_GCGC'y
Cuts at: 0 404 585 1155
Size: 404 181 570
Fragments arranged by size:
570 404 181
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 208 240 459 491 1155
Size: 208 32 219 32 664
Fragments arranged by size:
664 219 208 32 32
HincII GTy'rAC
Cuts at: 0 1076 1155
Size: 1076 79
MfeI C'AATT_G
Cuts at: 0 519 920 1155
Size: 519 401 235
Fragments arranged by size:
519 401 235
MscI TGG'CCA
Cuts at: 0 337 622 1155
Size: 337 285 533
Fragments arranged by size:
533 337 285
MslI CAynn'nnrTG
Cuts at: 0 596 616 962 1155
Size: 596 20 346 193
Fragments arranged by size:
596 346 193 20
MspA1I CmG'CkG
Cuts at: 0 240 1155
Size: 240 915
NcoI C'CATG_G
Cuts at: 0 914 1155
Size: 914 241
NheI G'CTAG_C
Cuts at: 0 787 1155
Size: 787 368
NspI r_CATG'y
Cuts at: 0 78 663 733 1155
Size: 78 585 70 422
Fragments arranged by size:
585 422 78 70
PciI A'CATG_T
Cuts at: 0 74 659 1155
Size: 74 585 496
Fragments arranged by size:
585 496 74
PfoI T'CCnGG_A
Cuts at: 0 698 1155
Size: 698 457
PshAI GACnn'nnGTC
Cuts at: 0 52 1155
Size: 52 1103
PvuII CAG'CTG
Cuts at: 0 240 1155
Size: 240 915
SacI G_AGCT'C
Cuts at: 0 950 1155
Size: 950 205
SapI GCTCTTCn'nnn_
Cuts at: 0 955 1155
Size: 955 200
ScaI AGT'ACT
Cuts at: 0 751 1155
Size: 751 404
SmlI C'TyrA_G
Cuts at: 0 227 886 1155
Size: 227 659 269
Fragments arranged by size:
659 269 227
StuI AGG'CCT
Cuts at: 0 382 434 1155
Size: 382 52 721
Fragments arranged by size:
721 382 52
StyI C'CwwG_G
Cuts at: 0 482 502 542 830 914 1155
Size: 482 20 40 288 84 241
Fragments arranged by size:
482 288 241 84 40 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 697 1155
Size: 697 458
TatI w'GTAC_w
Cuts at: 0 749 1155
Size: 749 406
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 736 768 1155
Size: 736 32 387
Fragments arranged by size:
736 387 32
Tth111I GACn'n_nGTC
Cuts at: 0 147 1155
Size: 147 1008
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 162 912 1155
Size: 162 750 243
Fragments arranged by size:
750 243 162
Enzymes that do cut:
AarI AcuI AfeI AflIII AgeI AloI ApoI AvaI
AvrII BanI BanII BbsI BlpI BmrI BmtI BpmI
BpuEI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstEII BstXI Bsu36I BtgI BtgZI BtsI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII
MfeI MscI MslI MspA1I NcoI NheI NspI PciI
PfoI PshAI PvuII SacI SapI ScaI SmlI StuI
StyI TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AhdI AleI AlfI
AlwNI ApaI ApaLI AscI AseI AsiSI BaeI BamHI
BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII
Bme1580I BmgBI BplI Bpu10I BsaI BsaAI BsaBI BsaHI
BsiEI BsiWI BsmBI BspHI BsrGI BstAPI BstBI BstYI
BstZ17I ClaI CspCI DraI DraIII DrdI EagI EciI
EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI
KpnI MluI MmeI NaeI NarI NdeI NgoMIV NotI
NruI NsiI PacI PasI PflMI PmeI PmlI PpiI
PpuMI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacII SalI SanDI SbfI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI XbaI XhoI XmaI XmnI ZraI