(Linear) (Six-base) MAPSORT of: KIEE2609.seq  Check: 8863  from: 1  to: 795



KIEE2609.orf

 With 182 enzymes: SgfI * 

                        February 12, 2016 18:20  ..

AflIII A'CryG_T

Cuts at:      0     369     795 
   Size:        369     426
 
ApaI G_GGCC'C

Cuts at:      0     281     795 
   Size:        281     514
 
BanI G'GyrC_C

Cuts at:      0     667     795 
   Size:        667     128
 
BanII G_rGCy'C

Cuts at:      0      53     281     795 
   Size:         53     228     514

  Fragments arranged by size: 

                 514     228      53
 
Bme1580I G_kGCm'C

Cuts at:      0     102     281     795 
   Size:        102     179     514

  Fragments arranged by size: 

                 514     179     102
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     655     679     795 
   Size:        655      24     116

  Fragments arranged by size: 

                 655     116      24
 
BsaAI yAC'GTr

Cuts at:      0     420     795 
   Size:        420     375
 
BsaWI w'CCGG_w

Cuts at:      0     468     795 
   Size:        468     327
 
BseYI C'CCAG_C

Cuts at:      0     742     778     795 
   Size:        742      36      17

  Fragments arranged by size: 

                 742      36      17
 
BsiEI CG_ry'CG

Cuts at:      0     294     795 
   Size:        294     501
 
BsmI GAATG_Cn'

Cuts at:      0      35     795 
   Size:         35     760
 
Bsp1286I G_dGCh'C

Cuts at:      0      53     102     281     795 
   Size:         53      49     179     514

  Fragments arranged by size: 

                 514     179      53      49
 
BspHI T'CATG_A

Cuts at:      0     761     795 
   Size:        761      34
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      95     795 
   Size:         95     700
 
BtsI GCAGTG_nn'

Cuts at:      0     684     795 
   Size:        684     111
 
EcoO109I rG'GnC_Cy

Cuts at:      0     150     277     596     634     795 
   Size:        150     127     319      38     161

  Fragments arranged by size: 

                 319     161     150     127      38
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     403     795 
   Size:        403     392
 
HaeII r_GCGC'y

Cuts at:      0     617     795 
   Size:        617     178
 
HincII GTy'rAC

Cuts at:      0     505     795 
   Size:        505     290
 
MspA1I CmG'CkG

Cuts at:      0     742     751     795 
   Size:        742       9      44

  Fragments arranged by size: 

                 742      44       9
 
NspI r_CATG'y

Cuts at:      0     223     795 
   Size:        223     572
 
PasI CC'CwG_GG

Cuts at:      0     274     795 
   Size:        274     521
 
PpuMI rG'GwC_Cy

Cuts at:      0     150     596     634     795 
   Size:        150     446      38     161

  Fragments arranged by size: 

                 446     161     150      38
 
PshAI GACnn'nnGTC

Cuts at:      0     751     795 
   Size:        751      44
 
PsiI TTA'TAA

Cuts at:      0     376     795 
   Size:        376     419
 
PspOMI G'GGCC_C

Cuts at:      0     277     795 
   Size:        277     518
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     511     543     795 
   Size:        511      32     252

  Fragments arranged by size: 

                 511     252      32
 
PvuII CAG'CTG

Cuts at:      0     742     795 
   Size:        742      53
 
ScaI AGT'ACT

Cuts at:      0     773     795 
   Size:        773      22
 
SnaBI TAC'GTA

Cuts at:      0     420     795 
   Size:        420     375
 
SphI G_CATG'C

Cuts at:      0     223     795 
   Size:        223     572
 
SspI AAT'ATT

Cuts at:      0     111     795 
   Size:        111     684
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     355     450     655     795 
   Size:        355      95     205     140

  Fragments arranged by size: 

                 355     205     140      95
 
TatI w'GTAC_w

Cuts at:      0     714     771     795 
   Size:        714      57      24

  Fragments arranged by size: 

                 714      57      24
 
Tth111I GACn'n_nGTC

Cuts at:      0     685     795 
   Size:        685     110
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     184     795 
   Size:        184     611
 

 Enzymes that do cut:

   AflIII     ApaI     BanI    BanII Bme1580I     BmrI    BsaAI    BsaWI
    BseYI    BsiEI     BsmI Bsp1286I    BspHI    BspMI     BtsI EcoO109I
     FalI    HaeII   HincII   MspA1I     NspI     PasI    PpuMI    PshAI
     PsiI   PspOMI     PsrI    PvuII     ScaI    SnaBI     SphI     SspI
    TaqII     TatI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaXI    BseRI     BsgI  BsiHKAI    BsiWI    BsmBI
    BspEI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FseI     FspI
    FspAI    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PspXI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI     SpeI     SrfI     StuI
     StyI     SwaI     TstI     XbaI     XhoI     XmaI     XmnI     ZraI