Restriction map for
pFN21AE2606
(Linear) (Six-base) MAPSORT of: KIEE2606.seq Check: 7690 from: 1 to: 2118
KIEE2606.orf
With 182 enzymes: SgfI *
February 12, 2016 18:20 ..
Acc65I G'GTAC_C
Cuts at: 0 1496 2118
Size: 1496 622
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1953 2118
Size: 1953 165
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1984 2016 2118
Size: 1984 32 102
Fragments arranged by size:
1984 102 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1199 1584 2118
Size: 1199 385 534
Fragments arranged by size:
1199 534 385
ApaI G_GGCC'C
Cuts at: 0 1323 1434 2118
Size: 1323 111 684
Fragments arranged by size:
1323 684 111
ApoI r'AATT_y
Cuts at: 0 307 1016 1666 2118
Size: 307 709 650 452
Fragments arranged by size:
709 650 452 307
AvaI C'yCGr_G
Cuts at: 0 386 1564 2118
Size: 386 1178 554
Fragments arranged by size:
1178 554 386
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1552 1585 2118
Size: 1552 33 533
Fragments arranged by size:
1552 533 33
BamHI G'GATC_C
Cuts at: 0 1841 2118
Size: 1841 277
BanI G'GyrC_C
Cuts at: 0 619 1496 1646 1708 2118
Size: 619 877 150 62 410
Fragments arranged by size:
877 619 410 150 62
BanII G_rGCy'C
Cuts at: 0 1323 1434 2118
Size: 1323 111 684
Fragments arranged by size:
1323 684 111
BbeI G_GCGC'C
Cuts at: 0 623 2118
Size: 623 1495
BbsI GAAGACnn'nnnn_
Cuts at: 0 1140 2057 2118
Size: 1140 917 61
Fragments arranged by size:
1140 917 61
BbvCI CC'TCA_GC
Cuts at: 0 449 2118
Size: 449 1669
BciVI GTATCCnnnnn_n'
Cuts at: 0 979 1057 1881 2118
Size: 979 78 824 237
Fragments arranged by size:
979 824 237 78
BclI T'GATC_A
Cuts at: 0 791 956 2118
Size: 791 165 1162
Fragments arranged by size:
1162 791 165
BglI GCCn_nnn'nGGC
Cuts at: 0 1054 1132 2118
Size: 1054 78 986
Fragments arranged by size:
1054 986 78
BglII A'GATC_T
Cuts at: 0 329 1181 1337 2118
Size: 329 852 156 781
Fragments arranged by size:
852 781 329 156
Bme1580I G_kGCm'C
Cuts at: 0 235 1323 1434 2118
Size: 235 1088 111 684
Fragments arranged by size:
1088 684 235 111
BmrI ACTGGGnnnn_n'
Cuts at: 0 1140 1437 1923 2118
Size: 1140 297 486 195
Fragments arranged by size:
1140 486 297 195
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 888 2118
Size: 888 1230
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1672 2044 2118
Size: 1672 372 74
Fragments arranged by size:
1672 372 74
Bpu10I CC'TnA_GC
Cuts at: 0 449 1492 2118
Size: 449 1043 626
Fragments arranged by size:
1043 626 449
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2059 2118
Size: 2059 59
BsaI GGTCTCn'nnnn_
Cuts at: 0 167 1225 2118
Size: 167 1058 893
Fragments arranged by size:
1058 893 167
BsaAI yAC'GTr
Cuts at: 0 141 365 2118
Size: 141 224 1753
Fragments arranged by size:
1753 224 141
BsaBI GATnn'nnATC
Cuts at: 0 793 1699 2118
Size: 793 906 419
Fragments arranged by size:
906 793 419
BsaHI Gr'CG_yC
Cuts at: 0 620 2118
Size: 620 1498
BsaWI w'CCGG_w
Cuts at: 0 1007 2118
Size: 1007 1111
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1492 1522 2118
Size: 1492 30 596
Fragments arranged by size:
1492 596 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1233 2118
Size: 1233 885
BseYI C'CCAG_C
Cuts at: 0 490 813 1326 1386 2118
Size: 490 323 513 60 732
Fragments arranged by size:
732 513 490 323 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 910 1564 1704 1903 2118
Size: 75 835 654 140 199 215
Fragments arranged by size:
835 654 215 199 140 75
BsiEI CG_ry'CG
Cuts at: 0 354 2045 2118
Size: 354 1691 73
Fragments arranged by size:
1691 354 73
BsiHKAI G_wGCw'C
Cuts at: 0 1078 2118
Size: 1078 1040
BsmI GAATG_Cn'
Cuts at: 0 378 1686 1740 2118
Size: 378 1308 54 378
Fragments arranged by size:
1308 378 378 54
BsmBI CGTCTCn'nnnn_
Cuts at: 0 556 693 2118
Size: 556 137 1425
Fragments arranged by size:
1425 556 137
Bsp1286I G_dGCh'C
Cuts at: 0 235 1078 1323 1434 2118
Size: 235 843 245 111 684
Fragments arranged by size:
843 684 245 235 111
BspEI T'CCGG_A
Cuts at: 0 1007 2118
Size: 1007 1111
BspHI T'CATG_A
Cuts at: 0 1619 1691 1751 2118
Size: 1619 72 60 367
Fragments arranged by size:
1619 367 72 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 932 1228 1460 2118
Size: 932 296 232 658
Fragments arranged by size:
932 658 296 232
BsrBI CCG'CTC
Cuts at: 0 12 1467 2118
Size: 12 1455 651
Fragments arranged by size:
1455 651 12
BsrDI GCAATG_nn'
Cuts at: 0 564 719 2118
Size: 564 155 1399
Fragments arranged by size:
1399 564 155
BsrFI r'CCGG_y
Cuts at: 0 868 1455 2118
Size: 868 587 663
Fragments arranged by size:
868 663 587
BssHII G'CGCG_C
Cuts at: 0 1523 2118
Size: 1523 595
BstEII G'GTnAC_C
Cuts at: 0 1095 2118
Size: 1095 1023
BstXI CCAn_nnnn'nTGG
Cuts at: 0 294 2118
Size: 294 1824
BstYI r'GATC_y
Cuts at: 0 329 1181 1337 1841 2118
Size: 329 852 156 504 277
Fragments arranged by size:
852 504 329 277 156
BtgI C'CryG_G
Cuts at: 0 725 2118
Size: 725 1393
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 745 1008 1107 2118
Size: 745 263 99 1011
Fragments arranged by size:
1011 745 263 99
BtsI GCAGTG_nn'
Cuts at: 0 831 1139 2118
Size: 831 308 979
Fragments arranged by size:
979 831 308
EaeI y'GGCC_r
Cuts at: 0 125 1367 1403 1482 1535 1727 2042 2118
Size: 125 1242 36 79 53 192 315 76
Fragments arranged by size:
1242 315 192 125 79 76 53 36
EagI C'GGCC_G
Cuts at: 0 2042 2118
Size: 2042 76
EarI CTCTTCn'nnn_
Cuts at: 0 1006 1474 1807 2118
Size: 1006 468 333 311
Fragments arranged by size:
1006 468 333 311
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1672 1953 2044 2118
Size: 1672 281 91 74
Fragments arranged by size:
1672 281 91 74
EcoNI CCTnn'n_nnAGG
Cuts at: 0 613 1728 2118
Size: 613 1115 390
Fragments arranged by size:
1115 613 390
EcoO109I rG'GnC_Cy
Cuts at: 0 70 1320 1409 1431 2019 2118
Size: 70 1250 89 22 588 99
Fragments arranged by size:
1250 588 99 89 70 22
EcoRI G'AATT_C
Cuts at: 0 307 1016 2118
Size: 307 709 1102
Fragments arranged by size:
1102 709 307
EcoRV GAT'ATC
Cuts at: 0 99 2118
Size: 99 2019
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2100 2118
Size: 2100 18
FspI TGC'GCA
Cuts at: 0 667 1438 2118
Size: 667 771 680
Fragments arranged by size:
771 680 667
FspAI rTGC'GCAy
Cuts at: 0 667 2118
Size: 667 1451
HaeII r_GCGC'y
Cuts at: 0 623 1387 1492 2118
Size: 623 764 105 626
Fragments arranged by size:
764 626 623 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1760 1792 2118
Size: 1760 32 326
Fragments arranged by size:
1760 326 32
KasI G'GCGC_C
Cuts at: 0 619 2118
Size: 619 1499
KpnI G_GTAC'C
Cuts at: 0 1500 2118
Size: 1500 618
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1187 2118
Size: 1187 931
MscI TGG'CCA
Cuts at: 0 127 1369 1537 2118
Size: 127 1242 168 581
Fragments arranged by size:
1242 581 168 127
MslI CAynn'nnrTG
Cuts at: 0 724 730 799 1555 1768 1987 2118
Size: 724 6 69 756 213 219 131
Fragments arranged by size:
756 724 219 213 131 69 6
MspA1I CmG'CkG
Cuts at: 0 1581 2110 2118
Size: 1581 529 8
Fragments arranged by size:
1581 529 8
NaeI GCC'GGC
Cuts at: 0 870 1457 2118
Size: 870 587 661
Fragments arranged by size:
870 661 587
NarI GG'CG_CC
Cuts at: 0 620 2118
Size: 620 1498
NcoI C'CATG_G
Cuts at: 0 725 2118
Size: 725 1393
NdeI CA'TA_TG
Cuts at: 0 103 2118
Size: 103 2015
NgoMIV G'CCGG_C
Cuts at: 0 868 1455 2118
Size: 868 587 663
Fragments arranged by size:
868 663 587
NruI TCG'CGA
Cuts at: 0 1027 2118
Size: 1027 1091
NspI r_CATG'y
Cuts at: 0 1986 2118
Size: 1986 132
PfoI T'CCnGG_A
Cuts at: 0 589 1778 2118
Size: 589 1189 340
Fragments arranged by size:
1189 589 340
PpuMI rG'GwC_Cy
Cuts at: 0 70 2019 2118
Size: 70 1949 99
Fragments arranged by size:
1949 99 70
PspOMI G'GGCC_C
Cuts at: 0 1319 1430 2118
Size: 1319 111 688
Fragments arranged by size:
1319 688 111
PspXI vC'TCGA_Gb
Cuts at: 0 1564 2118
Size: 1564 554
PstI C_TGCA'G
Cuts at: 0 1337 1596 2118
Size: 1337 259 522
Fragments arranged by size:
1337 522 259
SapI GCTCTTCn'nnn_
Cuts at: 0 1474 2118
Size: 1474 644
ScaI AGT'ACT
Cuts at: 0 400 847 2118
Size: 400 447 1271
Fragments arranged by size:
1271 447 400
SexAI A'CCwGG_T
Cuts at: 0 608 940 2118
Size: 608 332 1178
Fragments arranged by size:
1178 608 332
SfcI C'TryA_G
Cuts at: 0 146 1333 1592 2118
Size: 146 1187 259 526
Fragments arranged by size:
1187 526 259 146
SfoI GGC'GCC
Cuts at: 0 621 2118
Size: 621 1497
SmaI CCC'GGG
Cuts at: 0 388 2118
Size: 388 1730
SmlI C'TyrA_G
Cuts at: 0 1564 2074 2118
Size: 1564 510 44
Fragments arranged by size:
1564 510 44
SspI AAT'ATT
Cuts at: 0 827 2118
Size: 827 1291
StuI AGG'CCT
Cuts at: 0 713 2052 2118
Size: 713 1339 66
Fragments arranged by size:
1339 713 66
StyI C'CwwG_G
Cuts at: 0 500 725 2118
Size: 500 225 1393
Fragments arranged by size:
1393 500 225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 774 1879 2118
Size: 774 1105 239
Fragments arranged by size:
1105 774 239
TatI w'GTAC_w
Cuts at: 0 398 845 2118
Size: 398 447 1273
Fragments arranged by size:
1273 447 398
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1492 1524 2118
Size: 1492 32 594
Fragments arranged by size:
1492 594 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 498 1056 1377 2118
Size: 498 558 321 741
Fragments arranged by size:
741 558 498 321
XhoI C'TCGA_G
Cuts at: 0 1564 2118
Size: 1564 554
XmaI C'CCGG_G
Cuts at: 0 386 2118
Size: 386 1732
XmnI GAAnn'nnTTC
Cuts at: 0 1738 2118
Size: 1738 380
Enzymes that do cut:
Acc65I AcuI AloI AlwNI ApaI ApoI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BstEII BstXI BstYI
BtgI BtgZI BtsI EaeI EagI EarI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NruI NspI PfoI PpuMI PspOMI
PspXI PstI SapI ScaI SexAI SfcI SfoI SmaI
SmlI SspI StuI StyI TaqII TatI TstI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI ApaLI AscI AseI AsiSI AvrII
BcgI BfrBI BlpI BmgBI BmtI BsiWI BsrGI BssSI
BstAPI BstBI BstZ17I Bsu36I ClaI CspCI DraI DraIII
DrdI EciI EcoICRI FseI HincII HindIII HpaI MfeI
MluI NheI NotI NsiI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI
ZraI