Restriction map for
pFN21AE2605
(Linear) (Six-base) MAPSORT of: KIEE2605.seq Check: 3315 from: 1 to: 1785
KIEE2605.orf
With 182 enzymes: SgfI *
January 12, 2016 10:05 ..
AccI GT'mk_AC
Cuts at: 0 620 1785
Size: 620 1165
Acc65I G'GTAC_C
Cuts at: 0 329 1785
Size: 329 1456
AfeI AGC'GCT
Cuts at: 0 1483 1785
Size: 1483 302
AflIII A'CryG_T
Cuts at: 0 341 1785
Size: 341 1444
AleI CACnn'nnGTG
Cuts at: 0 649 1785
Size: 649 1136
AlwNI CAG_nnn'CTG
Cuts at: 0 786 1242 1785
Size: 786 456 543
Fragments arranged by size:
786 543 456
ApaI G_GGCC'C
Cuts at: 0 1127 1343 1785
Size: 1127 216 442
Fragments arranged by size:
1127 442 216
ApaLI G'TGCA_C
Cuts at: 0 1351 1785
Size: 1351 434
AvaI C'yCGr_G
Cuts at: 0 889 1052 1096 1485 1785
Size: 889 163 44 389 300
Fragments arranged by size:
889 389 300 163 44
BamHI G'GATC_C
Cuts at: 0 158 1785
Size: 158 1627
BanI G'GyrC_C
Cuts at: 0 271 329 610 1381 1785
Size: 271 58 281 771 404
Fragments arranged by size:
771 404 281 271 58
BanII G_rGCy'C
Cuts at: 0 389 1127 1343 1785
Size: 389 738 216 442
Fragments arranged by size:
738 442 389 216
BbeI G_GCGC'C
Cuts at: 0 614 1785
Size: 614 1171
BbsI GAAGACnn'nnnn_
Cuts at: 0 592 1462 1785
Size: 592 870 323
Fragments arranged by size:
870 592 323
BbvCI CC'TCA_GC
Cuts at: 0 415 1288 1785
Size: 415 873 497
Fragments arranged by size:
873 497 415
BciVI GTATCCnnnnn_n'
Cuts at: 0 705 1785
Size: 705 1080
BglI GCCn_nnn'nGGC
Cuts at: 0 1379 1785
Size: 1379 406
BglII A'GATC_T
Cuts at: 0 1223 1785
Size: 1223 562
Bme1580I G_kGCm'C
Cuts at: 0 1127 1343 1355 1785
Size: 1127 216 12 430
Fragments arranged by size:
1127 430 216 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 86 1262 1533 1785
Size: 86 1176 271 252
Fragments arranged by size:
1176 271 252 86
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 621 1434 1785
Size: 621 813 351
Fragments arranged by size:
813 621 351
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 1003 1588 1785
Size: 446 557 585 197
Fragments arranged by size:
585 557 446 197
Bpu10I CC'TnA_GC
Cuts at: 0 415 484 1243 1288 1785
Size: 415 69 759 45 497
Fragments arranged by size:
759 497 415 69 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 271 1759 1785
Size: 37 234 1488 26
Fragments arranged by size:
1488 234 37 26
BsaI GGTCTCn'nnnn_
Cuts at: 0 14 37 538 1194 1202 1785
Size: 14 23 501 656 8 583
Fragments arranged by size:
656 583 501 23 14 8
BsaAI yAC'GTr
Cuts at: 0 1527 1785
Size: 1527 258
BsaHI Gr'CG_yC
Cuts at: 0 611 1785
Size: 611 1174
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 419 449 1495 1525 1588 1618 1672 1702
Size: 419 30 1046 30 63 30 54 30
Cuts at: 1702 1785
Size: 83
Fragments arranged by size:
1046 419 83 63 54 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 613 751 1735 1785
Size: 613 138 984 50
Fragments arranged by size:
984 613 138 50
BseYI C'CCAG_C
Cuts at: 0 1626 1785
Size: 1626 159
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1516 1684 1785
Size: 1516 168 101
Fragments arranged by size:
1516 168 101
BsiEI CG_ry'CG
Cuts at: 0 1374 1785
Size: 1374 411
BsiHKAI G_wGCw'C
Cuts at: 0 449 1355 1785
Size: 449 906 430
Fragments arranged by size:
906 449 430
BsmI GAATG_Cn'
Cuts at: 0 660 1644 1785
Size: 660 984 141
Fragments arranged by size:
984 660 141
BsmBI CGTCTCn'nnnn_
Cuts at: 0 355 1785
Size: 355 1430
Bsp1286I G_dGCh'C
Cuts at: 0 389 449 1127 1343 1355 1785
Size: 389 60 678 216 12 430
Fragments arranged by size:
678 430 389 216 60 12
BspHI T'CATG_A
Cuts at: 0 1043 1785
Size: 1043 742
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 632 1706 1785
Size: 632 1074 79
Fragments arranged by size:
1074 632 79
BsrBI CCG'CTC
Cuts at: 0 483 1785
Size: 483 1302
BsrFI r'CCGG_y
Cuts at: 0 1363 1785
Size: 1363 422
BssSI C'ACGA_G
Cuts at: 0 645 1785
Size: 645 1140
BstEII G'GTnAC_C
Cuts at: 0 144 489 1785
Size: 144 345 1296
Fragments arranged by size:
1296 345 144
BstXI CCAn_nnnn'nTGG
Cuts at: 0 346 1431 1467 1785
Size: 346 1085 36 318
Fragments arranged by size:
1085 346 318 36
BstYI r'GATC_y
Cuts at: 0 158 1223 1785
Size: 158 1065 562
Fragments arranged by size:
1065 562 158
Bsu36I CC'TnA_GG
Cuts at: 0 606 1785
Size: 606 1179
BtgI C'CryG_G
Cuts at: 0 69 333 989 1257 1785
Size: 69 264 656 268 528
Fragments arranged by size:
656 528 268 264 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 948 1518 1785
Size: 948 570 267
Fragments arranged by size:
948 570 267
BtsI GCAGTG_nn'
Cuts at: 0 1353 1785
Size: 1353 432
DrdI GACnn_nn'nnGTC
Cuts at: 0 1261 1785
Size: 1261 524
EaeI y'GGCC_r
Cuts at: 0 336 799 1371 1529 1785
Size: 336 463 572 158 256
Fragments arranged by size:
572 463 336 256 158
EagI C'GGCC_G
Cuts at: 0 1371 1785
Size: 1371 414
EarI CTCTTCn'nnn_
Cuts at: 0 1753 1785
Size: 1753 32
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 1003 1588 1785
Size: 446 557 585 197
Fragments arranged by size:
585 557 446 197
EcoNI CCTnn'n_nnAGG
Cuts at: 0 52 1334 1785
Size: 52 1282 451
Fragments arranged by size:
1282 451 52
EcoO109I rG'GnC_Cy
Cuts at: 0 937 1124 1263 1339 1428 1572 1785
Size: 937 187 139 76 89 144 213
Fragments arranged by size:
937 213 187 144 139 89 76
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 744 1785
Size: 744 1041
HaeII r_GCGC'y
Cuts at: 0 614 1364 1485 1785
Size: 614 750 121 300
Fragments arranged by size:
750 614 300 121
KasI G'GCGC_C
Cuts at: 0 610 1785
Size: 610 1175
KpnI G_GTAC'C
Cuts at: 0 333 1785
Size: 333 1452
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 662 920 1580 1785
Size: 662 258 660 205
Fragments arranged by size:
662 660 258 205
MscI TGG'CCA
Cuts at: 0 338 1531 1785
Size: 338 1193 254
Fragments arranged by size:
1193 338 254
MslI CAynn'nnrTG
Cuts at: 0 649 1042 1348 1399 1405 1465 1525 1785
Size: 649 393 306 51 6 60 60 260
Fragments arranged by size:
649 393 306 260 60 60 51 6
MspA1I CmG'CkG
Cuts at: 0 297 798 930 1197 1314 1740 1785
Size: 297 501 132 267 117 426 45
Fragments arranged by size:
501 426 297 267 132 117 45
NaeI GCC'GGC
Cuts at: 0 1365 1785
Size: 1365 420
NarI GG'CG_CC
Cuts at: 0 611 1785
Size: 611 1174
NcoI C'CATG_G
Cuts at: 0 333 1257 1785
Size: 333 924 528
Fragments arranged by size:
924 528 333
NgoMIV G'CCGG_C
Cuts at: 0 1363 1785
Size: 1363 422
NspI r_CATG'y
Cuts at: 0 345 1110 1404 1785
Size: 345 765 294 381
Fragments arranged by size:
765 381 345 294
PciI A'CATG_T
Cuts at: 0 341 1785
Size: 341 1444
PflMI CCAn_nnn'nTGG
Cuts at: 0 786 1785
Size: 786 999
PfoI T'CCnGG_A
Cuts at: 0 1295 1785
Size: 1295 490
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1752 1784 1785
Size: 1752 32 1
Fragments arranged by size:
1752 32 1
PpuMI rG'GwC_Cy
Cuts at: 0 1263 1572 1785
Size: 1263 309 213
Fragments arranged by size:
1263 309 213
PspOMI G'GGCC_C
Cuts at: 0 1123 1339 1785
Size: 1123 216 446
Fragments arranged by size:
1123 446 216
PstI C_TGCA'G
Cuts at: 0 260 374 869 1360 1580 1785
Size: 260 114 495 491 220 205
Fragments arranged by size:
495 491 260 220 205 114
PvuII CAG'CTG
Cuts at: 0 930 1785
Size: 930 855
SanDI GG'GwC_CC
Cuts at: 0 1263 1785
Size: 1263 522
SapI GCTCTTCn'nnn_
Cuts at: 0 1753 1785
Size: 1753 32
SbfI CC_TGCA'GG
Cuts at: 0 260 869 1785
Size: 260 609 916
Fragments arranged by size:
916 609 260
ScaI AGT'ACT
Cuts at: 0 232 1785
Size: 232 1553
SexAI A'CCwGG_T
Cuts at: 0 566 1785
Size: 566 1219
SfcI C'TryA_G
Cuts at: 0 256 370 865 1356 1576 1785
Size: 256 114 495 491 220 209
Fragments arranged by size:
495 491 256 220 209 114
SfoI GGC'GCC
Cuts at: 0 612 1785
Size: 612 1173
SmaI CCC'GGG
Cuts at: 0 891 1785
Size: 891 894
SmlI C'TyrA_G
Cuts at: 0 52 286 1774 1785
Size: 52 234 1488 11
Fragments arranged by size:
1488 234 52 11
SpeI A'CTAG_T
Cuts at: 0 316 1785
Size: 316 1469
SphI G_CATG'C
Cuts at: 0 1110 1785
Size: 1110 675
StuI AGG'CCT
Cuts at: 0 604 1654 1785
Size: 604 1050 131
Fragments arranged by size:
1050 604 131
StyI C'CwwG_G
Cuts at: 0 333 377 461 719 1257 1424 1649 1785
Size: 333 44 84 258 538 167 225 136
Fragments arranged by size:
538 333 258 225 167 136 84 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 508 523 1785
Size: 508 15 1262
Fragments arranged by size:
1262 508 15
TatI w'GTAC_w
Cuts at: 0 230 1511 1785
Size: 230 1281 274
Fragments arranged by size:
1281 274 230
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 419 451 1785
Size: 419 32 1334
Fragments arranged by size:
1334 419 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 463 1056 1428 1785
Size: 463 593 372 357
Fragments arranged by size:
593 463 372 357
XmaI C'CCGG_G
Cuts at: 0 889 1785
Size: 889 896
Enzymes that do cut:
AccI Acc65I AfeI AflIII AleI AlwNI ApaI ApaLI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI
BssSI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I FalI
HaeII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PpiI
PpuMI PspOMI PstI PvuII SanDI SapI SbfI ScaI
SexAI SfcI SfoI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AcuI AflII AgeI AhdI AlfI
AloI ApoI AscI AseI AsiSI AvrII BaeI BcgI
BclI BfrBI BlpI BmgBI BmtI BsaBI BsaWI BsiWI
BspEI BsrDI BsrGI BssHII BstAPI BstBI BstZ17I ClaI
CspCI DraI DraIII EciI EcoICRI EcoRI EcoRV FseI
FspI FspAI Hin4I HincII HindIII HpaI MfeI MluI
NdeI NheI NotI NruI NsiI PacI PasI PmeI
PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SfiI SgfI SgrAI SnaBI SrfI SspI
SwaI Tth111I XbaI XhoI XmnI ZraI