Restriction map for
pFN21AE2594
(Linear) (Six-base) MAPSORT of: KIEE2594.seq Check: 6642 from: 1 to: 10992
KIEE2594.orf
With 182 enzymes: SgfI *
January 6, 2016 09:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3265 6742 10992
Size: 3265 3477 4250
Fragments arranged by size:
4250 3477 3265
AatII G_ACGT'C
Cuts at: 0 2199 10992
Size: 2199 8793
AccI GT'mk_AC
Cuts at: 0 5099 7534 10992
Size: 5099 2435 3458
Fragments arranged by size:
5099 3458 2435
AclI AA'CG_TT
Cuts at: 0 10838 10992
Size: 10838 154
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 392 1082 1103 2489 2734 3485 4019
Size: 149 243 690 21 1386 245 751 534
Cuts at: 4019 5426 5797 8453 8902 10058 10525 10643 10992
Size: 1407 371 2656 449 1156 467 118 349
Fragments arranged by size:
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449 371 349 245 243 149 118 21
AfeI AGC'GCT
Cuts at: 0 1378 2350 9347 10992
Size: 1378 972 6997 1645
Fragments arranged by size:
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AflIII A'CryG_T
Cuts at: 0 3062 6025 6455 9603 10992
Size: 3062 2963 430 3148 1389
Fragments arranged by size:
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AgeI A'CCGG_T
Cuts at: 0 7314 9917 10992
Size: 7314 2603 1075
Fragments arranged by size:
7314 2603 1075
AleI CACnn'nnGTG
Cuts at: 0 2683 6046 8128 10992
Size: 2683 3363 2082 2864
Fragments arranged by size:
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AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 9205 10119 10168 10992
Size: 9205 914 49 824
Fragments arranged by size:
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AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2376 2408 3762 3794 10992
Size: 2376 32 1354 32 7198
Fragments arranged by size:
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AlwNI CAG_nnn'CTG
Cuts at: 0 771 946 4864 5257 5347 5404 5434 6765
Size: 771 175 3918 393 90 57 30 1331
Cuts at: 6765 7681 9514 9840 10533 10561 10992
Size: 916 1833 326 693 28 431
Fragments arranged by size:
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326 175 90 57 30 28
ApaI G_GGCC'C
Cuts at: 0 6055 8066 9014 9399 10030 10634 10992
Size: 6055 2011 948 385 631 604 358
Fragments arranged by size:
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ApaLI G'TGCA_C
Cuts at: 0 173 6755 9886 10992
Size: 173 6582 3131 1106
Fragments arranged by size:
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ApoI r'AATT_y
Cuts at: 0 107 1072 1081 1261 1291 3128 3922 3993
Size: 107 965 9 180 30 1837 794 71
Cuts at: 3993 4288 4416 7227 7896 8536 10429 10992
Size: 295 128 2811 669 640 1893 563
Fragments arranged by size:
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295 180 128 107 71 30 9
AvaI C'yCGr_G
Cuts at: 0 40 252 263 518 674 1955 2030 2642
Size: 40 212 11 255 156 1281 75 612
Cuts at: 2642 5212 5573 8734 9006 9091 9359 9466 9500
Size: 2570 361 3161 272 85 268 107 34
Cuts at: 9500 10992
Size: 1492
Fragments arranged by size:
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255 212 156 107 85 75 40 34
11
AvrII C'CTAG_G
Cuts at: 0 607 3835 10992
Size: 607 3228 7157
Fragments arranged by size:
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BamHI G'GATC_C
Cuts at: 0 3819 7463 10992
Size: 3819 3644 3529
Fragments arranged by size:
3819 3644 3529
BanI G'GyrC_C
Cuts at: 0 244 4131 4992 5699 6992 9654 10992
Size: 244 3887 861 707 1293 2662 1338
Fragments arranged by size:
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BanII G_rGCy'C
Cuts at: 0 545 615 992 4769 5768 6055 6079 7480
Size: 545 70 377 3777 999 287 24 1401
Cuts at: 7480 7530 8023 8066 8404 8741 9014 9366 9399
Size: 50 493 43 338 337 273 352 33
Cuts at: 9399 10030 10049 10634 10992
Size: 631 19 585 358
Fragments arranged by size:
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358 352 338 337 287 273 70 50
43 33 24 19
BbeI G_GCGC'C
Cuts at: 0 4996 5703 6996 10992
Size: 4996 707 1293 3996
Fragments arranged by size:
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BbsI GAAGACnn'nnnn_
Cuts at: 0 2495 3386 3397 4241 4972 5020 6458 8473
Size: 2495 891 11 844 731 48 1438 2015
Cuts at: 8473 10992
Size: 2519
Fragments arranged by size:
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11
BbvCI CC'TCA_GC
Cuts at: 0 3088 6098 6545 6873 9677 10061 10992
Size: 3088 3010 447 328 2804 384 931
Fragments arranged by size:
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BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1592 1626 3468 3502 10992
Size: 1592 34 1842 34 7490
Fragments arranged by size:
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BciVI GTATCCnnnnn_n'
Cuts at: 0 9814 10992
Size: 9814 1178
BclI T'GATC_A
Cuts at: 0 3360 10992
Size: 3360 7632
BglI GCCn_nnn'nGGC
Cuts at: 0 6571 9670 10819 10992
Size: 6571 3099 1149 173
Fragments arranged by size:
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BglII A'GATC_T
Cuts at: 0 4113 6235 6735 10880 10992
Size: 4113 2122 500 4145 112
Fragments arranged by size:
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BlpI GC'TnA_GC
Cuts at: 0 5348 10992
Size: 5348 5644
Bme1580I G_kGCm'C
Cuts at: 0 177 6055 6759 7283 8066 8149 9014 9194
Size: 177 5878 704 524 783 83 865 180
Cuts at: 9194 9399 9890 10030 10266 10634 10992
Size: 205 491 140 236 368 358
Fragments arranged by size:
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236 205 180 177 140 83
BmgBI CAC'GTC
Cuts at: 0 3063 6028 6456 10992
Size: 3063 2965 428 4536
Fragments arranged by size:
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BmrI ACTGGGnnnn_n'
Cuts at: 0 629 738 954 3271 3779 6399 6483 9181
Size: 629 109 216 2317 508 2620 84 2698
Cuts at: 9181 9189 9311 9815 10992
Size: 8 122 504 1177
Fragments arranged by size:
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122 109 84 8
BmtI G_CTAG'C
Cuts at: 0 779 1463 10992
Size: 779 684 9529
Fragments arranged by size:
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BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 278 10992
Size: 278 10714
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 819 899 1898 2231 2953 5411 6231 6532
Size: 819 80 999 333 722 2458 820 301
Cuts at: 6532 6740 6836 7529 7575 7748 7817 8111 9189
Size: 208 96 693 46 173 69 294 1078
Cuts at: 9189 9817 9876 10684 10992
Size: 628 59 808 308
Fragments arranged by size:
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628 333 308 301 294 208 173 96
80 69 59 46
Bpu10I CC'TnA_GC
Cuts at: 0 2897 3088 6098 6545 6873 7204 8403 9268
Size: 2897 191 3010 447 328 331 1199 865
Cuts at: 9268 9677 10022 10061 10238 10992
Size: 409 345 39 177 754
Fragments arranged by size:
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331 328 191 177 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1501 2875 3216 3629 5539 5908 7070 7316
Size: 1501 1374 341 413 1910 369 1162 246
Cuts at: 7316 8597 10142 10528 10768 10992
Size: 1281 1545 386 240 224
Fragments arranged by size:
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369 341 246 240 224
BsaI GGTCTCn'nnnn_
Cuts at: 0 209 1705 2138 2760 3863 8899 10473 10992
Size: 209 1496 433 622 1103 5036 1574 519
Fragments arranged by size:
5036 1574 1496 1103 622 519 433 209
BsaAI yAC'GTr
Cuts at: 0 4246 10872 10992
Size: 4246 6626 120
Fragments arranged by size:
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BsaBI GATnn'nnATC
Cuts at: 0 3818 10992
Size: 3818 7174
BsaHI Gr'CG_yC
Cuts at: 0 2196 4993 5333 5700 6993 10992
Size: 2196 2797 340 367 1293 3999
Fragments arranged by size:
3999 2797 2196 1293 367 340
BsaWI w'CCGG_w
Cuts at: 0 2282 2394 7314 9917 10992
Size: 2282 112 4920 2603 1075
Fragments arranged by size:
4920 2603 2282 1075 112
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 552 582 1376 1406 7527 7557 7573 7603
Size: 552 30 794 30 6121 30 16 30
Cuts at: 7603 7746 7776 8768 8798 10837 10867 10992
Size: 143 30 992 30 2039 30 125
Fragments arranged by size:
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30 30 30 30 30 30 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 149 338 752 2169 2271 3656 5561 5902
Size: 149 189 414 1417 102 1385 1905 341
Cuts at: 5902 5905 6115 6118 7169 7172 7339 7531 7919
Size: 3 210 3 1051 3 167 192 388
Cuts at: 7919 9065 9347 9484 10022 10049 10992
Size: 1146 282 137 538 27 943
Fragments arranged by size:
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388 341 282 210 192 189 167 149
137 102 27 3 3 3
BseYI C'CCAG_C
Cuts at: 0 1521 3238 5267 5397 6699 7304 7384 8059
Size: 1521 1717 2029 130 1302 605 80 675
Cuts at: 8059 8971 9013 9021 9124 9377 9429 9455 9953
Size: 912 42 8 103 253 52 26 498
Cuts at: 9953 10088 10115 10212 10393 10992
Size: 135 27 97 181 599
Fragments arranged by size:
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498 253 181 135 130 103 97 80
52 42 27 26 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 932 1123 3696 6602 8210 8231 9428 9538
Size: 932 191 2573 2906 1608 21 1197 110
Cuts at: 9538 9541 9898 10079 10090 10585 10992
Size: 3 357 181 11 495 407
Fragments arranged by size:
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191 181 110 21 11 3
BsiEI CG_ry'CG
Cuts at: 0 5947 8385 10992
Size: 5947 2438 2607
Fragments arranged by size:
5947 2607 2438
BsiHKAI G_wGCw'C
Cuts at: 0 177 4769 6079 6759 8053 8447 9890 10992
Size: 177 4592 1310 680 1294 394 1443 1102
Fragments arranged by size:
4592 1443 1310 1294 1102 680 394 177
BsmI GAATG_Cn'
Cuts at: 0 7350 8001 8160 8202 10992
Size: 7350 651 159 42 2790
Fragments arranged by size:
7350 2790 651 159 42
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2222 2409 5040 7983 10584 10992
Size: 2222 187 2631 2943 2601 408
Fragments arranged by size:
2943 2631 2601 2222 408 187
Bsp1286I G_dGCh'C
Cuts at: 0 177 545 615 992 4769 5768 6055 6079
Size: 177 368 70 377 3777 999 287 24
Cuts at: 6079 6759 7283 7480 7530 8023 8053 8066 8149
Size: 680 524 197 50 493 30 13 83
Cuts at: 8149 8404 8447 8741 9014 9194 9366 9399 9890
Size: 255 43 294 273 180 172 33 491
Cuts at: 9890 10030 10049 10266 10634 10992
Size: 140 19 217 368 358
Fragments arranged by size:
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368 358 294 287 273 255 217 197
180 177 172 140 83 70 50 43
33 30 24 19 13
BspEI T'CCGG_A
Cuts at: 0 2394 10992
Size: 2394 8598
BspHI T'CATG_A
Cuts at: 0 588 10970 10992
Size: 588 10382 22
Fragments arranged by size:
10382 588 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3265 3339 5073 5111 5919 6742 10230 10814
Size: 3265 74 1734 38 808 823 3488 584
Cuts at: 10814 10992
Size: 178
Fragments arranged by size:
3488 3265 1734 823 808 584 178 74
38
BsrBI CCG'CTC
Cuts at: 0 390 525 702 5750 10992
Size: 390 135 177 5048 5242
Fragments arranged by size:
5242 5048 390 177 135
BsrDI GCAATG_nn'
Cuts at: 0 7670 7838 8531 10979 10992
Size: 7670 168 693 2448 13
Fragments arranged by size:
7670 2448 693 168 13
BsrFI r'CCGG_y
Cuts at: 0 5870 6198 6877 7314 8922 9917 10992
Size: 5870 328 679 437 1608 995 1075
Fragments arranged by size:
5870 1608 1075 995 679 437 328
BssSI C'ACGA_G
Cuts at: 0 371 3625 4370 10992
Size: 371 3254 745 6622
Fragments arranged by size:
6622 3254 745 371
BstAPI GCAn_nnn'nTGC
Cuts at: 0 946 5053 8197 8299 8629 9885 10533 10992
Size: 946 4107 3144 102 330 1256 648 459
Fragments arranged by size:
4107 3144 1256 946 648 459 330 102
BstBI TT'CG_AA
Cuts at: 0 1576 1908 4095 4422 10992
Size: 1576 332 2187 327 6570
Fragments arranged by size:
6570 2187 1576 332 327
BstEII G'GTnAC_C
Cuts at: 0 728 5173 5734 7374 9864 10992
Size: 728 4445 561 1640 2490 1128
Fragments arranged by size:
4445 2490 1640 1128 728 561
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1381 6048 6504 8526 10992
Size: 1381 4667 456 2022 2466
Fragments arranged by size:
4667 2466 2022 1381 456
BstYI r'GATC_y
Cuts at: 0 124 513 829 2928 3819 4113 4676 5247
Size: 124 389 316 2099 891 294 563 571
Cuts at: 5247 6235 6735 7463 8507 10880 10992
Size: 988 500 728 1044 2373 112
Fragments arranged by size:
2373 2099 1044 988 891 728 571 563
500 389 316 294 124 112
Bsu36I CC'TnA_GG
Cuts at: 0 1928 5933 6032 7600 10992
Size: 1928 4005 99 1568 3392
Fragments arranged by size:
4005 3392 1928 1568 99
BtgI C'CryG_G
Cuts at: 0 5729 6080 6521 7522 8138 9574 9635 9870
Size: 5729 351 441 1001 616 1436 61 235
Cuts at: 9870 10484 10992
Size: 614 508
Fragments arranged by size:
5729 1436 1001 616 614 508 441 351
235 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 6516 8573 10992
Size: 6516 2057 2419
Fragments arranged by size:
6516 2419 2057
BtsI GCAGTG_nn'
Cuts at: 0 628 879 888 1788 5336 5501 6263 6921
Size: 628 251 9 900 3548 165 762 658
Cuts at: 6921 7411 8198 8219 8300 10992
Size: 490 787 21 81 2692
Fragments arranged by size:
3548 2692 900 787 762 658 628 490
251 165 81 21 9
ClaI AT'CG_AT
Cuts at: 0 3488 10992
Size: 3488 7504
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 7653 7688 10992
Size: 7653 35 3304
Fragments arranged by size:
7653 3304 35
DraI TTT'AAA
Cuts at: 0 2978 3920 10992
Size: 2978 942 7072
Fragments arranged by size:
7072 2978 942
DraIII CAC_nnn'GTG
Cuts at: 0 6933 9406 10992
Size: 6933 2473 1586
Fragments arranged by size:
6933 2473 1586
DrdI GACnn_nn'nnGTC
Cuts at: 0 10330 10992
Size: 10330 662
EaeI y'GGCC_r
Cuts at: 0 87 3968 6650 8261 8516 8639 8924 9337
Size: 87 3881 2682 1611 255 123 285 413
Cuts at: 9337 10131 10743 10992
Size: 794 612 249
Fragments arranged by size:
3881 2682 1611 794 612 413 285 255
249 123 87
EarI CTCTTCn'nnn_
Cuts at: 0 50 1118 1842 3905 4368 4547 5003 5521
Size: 50 1068 724 2063 463 179 456 518
Cuts at: 5521 5879 7556 10343 10992
Size: 358 1677 2787 649
Fragments arranged by size:
2787 2063 1677 1068 724 649 518 463
456 358 179 50
EcoICRI GAG'CTC
Cuts at: 0 4767 6077 10992
Size: 4767 1310 4915
Fragments arranged by size:
4915 4767 1310
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 392 819 899 1082 1103 1898 2231
Size: 149 243 427 80 183 21 795 333
Cuts at: 2231 2489 2734 2953 3485 4019 5411 5426 5797
Size: 258 245 219 532 534 1392 15 371
Cuts at: 5797 6231 6532 6740 6836 7529 7575 7748 7817
Size: 434 301 208 96 693 46 173 69
Cuts at: 7817 8111 8453 8902 9189 9817 9876 10058 10525
Size: 294 342 449 287 628 59 182 467
Cuts at: 10525 10643 10684 10992
Size: 118 41 308
Fragments arranged by size:
1392 795 693 628 534 532 467 449
434 427 371 342 333 308 301 294
287 258 245 243 219 208 183 182
173 149 118 96 80 69 59 46
41 21 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 7705 9280 10037 10992
Size: 7705 1575 757 955
Fragments arranged by size:
7705 1575 955 757
EcoO109I rG'GnC_Cy
Cuts at: 0 337 800 5216 5954 6052 6588 7710 8008
Size: 337 463 4416 738 98 536 1122 298
Cuts at: 8008 8083 8608 8913 9010 9395 9396 9672 10026
Size: 75 525 305 97 385 1 276 354
Cuts at: 10026 10027 10181 10530 10630 10631 10992
Size: 1 154 349 100 1 361
Fragments arranged by size:
4416 1122 738 536 525 463 385 361
354 349 337 305 298 276 154 100
98 97 75 1 1 1
EcoRI G'AATT_C
Cuts at: 0 3993 7227 10429 10992
Size: 3993 3234 3202 563
Fragments arranged by size:
3993 3234 3202 563
EcoRV GAT'ATC
Cuts at: 0 654 3708 10992
Size: 654 3054 7284
Fragments arranged by size:
7284 3054 654
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1078 1213 2722 2887 3148 4357 5651 6988
Size: 1078 135 1509 165 261 1209 1294 1337
Cuts at: 6988 7664 7929 10809 10992
Size: 676 265 2880 183
Fragments arranged by size:
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261 183 165 135
HaeII r_GCGC'y
Cuts at: 0 1380 2352 2961 4996 5703 6766 6996 8495
Size: 1380 972 609 2035 707 1063 230 1499
Cuts at: 8495 9148 9349 10992
Size: 653 201 1643
Fragments arranged by size:
2035 1643 1499 1380 1063 972 707 653
609 230 201
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 552 584 1119 1151 3052 3084 3602 3634
Size: 552 32 535 32 1901 32 518 32
Cuts at: 3634 4387 4419 6322 6354 6382 6414 6790 6822
Size: 753 32 1903 32 28 32 376 32
Cuts at: 6822 7216 7248 7573 7605 8367 8386 8399 8418
Size: 394 32 325 32 762 19 13 19
Cuts at: 8418 8704 8736 10005 10037 10992
Size: 286 32 1269 32 955
Fragments arranged by size:
1903 1901 1269 955 762 753 552 535
518 394 376 325 286 32 32 32
32 32 32 32 32 32 32 32
32 28 19 19 13
HincII GTy'rAC
Cuts at: 0 198 4449 7581 7728 8309 8994 10992
Size: 198 4251 3132 147 581 685 1998
Fragments arranged by size:
4251 3132 1998 685 581 198 147
HindIII A'AGCT_T
Cuts at: 0 2704 4334 5590 8875 10992
Size: 2704 1630 1256 3285 2117
Fragments arranged by size:
3285 2704 2117 1630 1256
KasI G'GCGC_C
Cuts at: 0 4992 5699 6992 10992
Size: 4992 707 1293 4000
Fragments arranged by size:
4992 4000 1293 707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3816 5230 7100 7288 10976 10992
Size: 3816 1414 1870 188 3688 16
Fragments arranged by size:
3816 3688 1870 1414 188 16
MscI TGG'CCA
Cuts at: 0 3970 8641 10992
Size: 3970 4671 2351
Fragments arranged by size:
4671 3970 2351
MslI CAynn'nnrTG
Cuts at: 0 2683 3812 4774 6046 8128 8371 9166 9187
Size: 2683 1129 962 1272 2082 243 795 21
Cuts at: 9187 9602 10992
Size: 415 1390
Fragments arranged by size:
2683 2082 1390 1272 1129 962 795 415
243 21
MspA1I CmG'CkG
Cuts at: 0 815 1702 1776 3345 5088 5491 5827 5971
Size: 815 887 74 1569 1743 403 336 144
Cuts at: 5971 8182 8227 8484 8838 9052 9369 9433 9637
Size: 2211 45 257 354 214 317 64 204
Cuts at: 9637 9896 10018 10216 10558 10677 10992
Size: 259 122 198 342 119 315
Fragments arranged by size:
2211 1743 1569 887 815 403 354 342
336 317 315 259 257 214 204 198
144 122 119 74 64 45
NaeI GCC'GGC
Cuts at: 0 5872 6879 8924 10992
Size: 5872 1007 2045 2068
Fragments arranged by size:
5872 2068 2045 1007
NarI GG'CG_CC
Cuts at: 0 4993 5700 6993 10992
Size: 4993 707 1293 3999
Fragments arranged by size:
4993 3999 1293 707
NcoI C'CATG_G
Cuts at: 0 5729 6080 9870 10992
Size: 5729 351 3790 1122
Fragments arranged by size:
5729 3790 1122 351
NgoMIV G'CCGG_C
Cuts at: 0 5870 6877 8922 10992
Size: 5870 1007 2045 2070
Fragments arranged by size:
5870 2070 2045 1007
NheI G'CTAG_C
Cuts at: 0 775 1459 10992
Size: 775 684 9533
Fragments arranged by size:
9533 775 684
NruI TCG'CGA
Cuts at: 0 617 10992
Size: 617 10375
NspI r_CATG'y
Cuts at: 0 1212 1542 9607 10992
Size: 1212 330 8065 1385
Fragments arranged by size:
8065 1385 1212 330
PasI CC'CwG_GG
Cuts at: 0 6305 8359 8772 8799 9282 9629 10992
Size: 6305 2054 413 27 483 347 1363
Fragments arranged by size:
6305 2054 1363 483 413 347 27
PciI A'CATG_T
Cuts at: 0 9603 10992
Size: 9603 1389
PflMI CCAn_nnn'nTGG
Cuts at: 0 10523 10992
Size: 10523 469
PfoI T'CCnGG_A
Cuts at: 0 5 2208 6717 6815 10003 10992
Size: 5 2203 4509 98 3188 989
Fragments arranged by size:
4509 3188 2203 989 98 5
PpuMI rG'GwC_Cy
Cuts at: 0 800 5954 8913 10181 10992
Size: 800 5154 2959 1268 811
Fragments arranged by size:
5154 2959 1268 811 800
PshAI GACnn'nnGTC
Cuts at: 0 8384 8911 9613 10992
Size: 8384 527 702 1379
Fragments arranged by size:
8384 1379 702 527
PsiI TTA'TAA
Cuts at: 0 233 10992
Size: 233 10759
PspOMI G'GGCC_C
Cuts at: 0 6051 8062 9010 9395 10026 10630 10992
Size: 6051 2011 948 385 631 604 362
Fragments arranged by size:
6051 2011 948 631 604 385 362
PspXI vC'TCGA_Gb
Cuts at: 0 1955 2642 9500 10992
Size: 1955 687 6858 1492
Fragments arranged by size:
6858 1955 1492 687
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1406 1438 4276 4308 10992
Size: 1406 32 2838 32 6684
Fragments arranged by size:
6684 2838 1406 32 32
PstI C_TGCA'G
Cuts at: 0 939 2910 3350 5343 5496 5655 5826 5916
Size: 939 1971 440 1993 153 159 171 90
Cuts at: 5916 5970 6318 6770 7431 8625 9180 9374 10055
Size: 54 348 452 661 1194 555 194 681
Cuts at: 10055 10811 10880 10992
Size: 756 69 112
Fragments arranged by size:
1993 1971 1194 939 756 681 661 555
452 440 348 194 171 159 153 112
90 69 54
PvuI CG_AT'CG
Cuts at: 0 8385 10992
Size: 8385 2607
PvuII CAG'CTG
Cuts at: 0 1702 1776 3345 5088 5491 5971 8838 9052
Size: 1702 74 1569 1743 403 480 2867 214
Cuts at: 9052 9433 10018 10216 10558 10677 10992
Size: 381 585 198 342 119 315
Fragments arranged by size:
2867 1743 1702 1569 585 480 403 381
342 315 214 198 119 74
SacI G_AGCT'C
Cuts at: 0 4769 6079 10992
Size: 4769 1310 4913
Fragments arranged by size:
4913 4769 1310
SacII CC_GC'GG
Cuts at: 0 9638 10992
Size: 9638 1354
SanDI GG'GwC_CC
Cuts at: 0 10181 10992
Size: 10181 811
SapI GCTCTTCn'nnn_
Cuts at: 0 4547 7556 10992
Size: 4547 3009 3436
Fragments arranged by size:
4547 3436 3009
SbfI CC_TGCA'GG
Cuts at: 0 8625 10811 10992
Size: 8625 2186 181
Fragments arranged by size:
8625 2186 181
ScaI AGT'ACT
Cuts at: 0 9132 10992
Size: 9132 1860
SexAI A'CCwGG_T
Cuts at: 0 4122 10992
Size: 4122 6870
SfcI C'TryA_G
Cuts at: 0 507 657 935 1037 2295 2906 3346 5339
Size: 507 150 278 102 1258 611 440 1993
Cuts at: 5339 5492 5651 5822 5912 5966 6314 6766 6833
Size: 153 159 171 90 54 348 452 67
Cuts at: 6833 7052 7427 8621 8984 9176 9327 9370 10051
Size: 219 375 1194 363 192 151 43 681
Cuts at: 10051 10807 10876 10992
Size: 756 69 116
Fragments arranged by size:
1993 1258 1194 756 681 611 507 452
440 375 363 348 278 219 192 171
159 153 151 150 116 102 90 69
67 54 43
SfoI GGC'GCC
Cuts at: 0 4994 5701 6994 10992
Size: 4994 707 1293 3998
Fragments arranged by size:
4994 3998 1293 707
SgrAI Cr'CCGG_yG
Cuts at: 0 5870 7314 10992
Size: 5870 1444 3678
Fragments arranged by size:
5870 3678 1444
SmaI CCC'GGG
Cuts at: 0 676 5214 9093 10992
Size: 676 4538 3879 1899
Fragments arranged by size:
4538 3879 1899 676
SmlI C'TyrA_G
Cuts at: 0 518 1516 1955 2642 2890 3231 3644 5554
Size: 518 998 439 687 248 341 413 1910
Cuts at: 5554 5923 7085 7331 8612 9500 10157 10543 10783
Size: 369 1162 246 1281 888 657 386 240
Cuts at: 10783 10992
Size: 209
Fragments arranged by size:
1910 1281 1162 998 888 687 657 518
439 413 386 369 341 248 246 240
209
SpeI A'CTAG_T
Cuts at: 0 9531 10992
Size: 9531 1461
SphI G_CATG'C
Cuts at: 0 1212 1542 10992
Size: 1212 330 9450
Fragments arranged by size:
9450 1212 330
StuI AGG'CCT
Cuts at: 0 5856 6550 6849 7570 10992
Size: 5856 694 299 721 3422
Fragments arranged by size:
5856 3422 721 694 299
StyI C'CwwG_G
Cuts at: 0 607 1271 1992 3835 5729 5903 5950 5985
Size: 607 664 721 1843 1894 174 47 35
Cuts at: 5985 6080 6089 6893 7913 8723 8909 9109 9870
Size: 95 9 804 1020 810 186 200 761
Cuts at: 9870 10992
Size: 1122
Fragments arranged by size:
1894 1843 1122 1020 810 804 761 721
664 607 200 186 174 95 47 35
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 15 183 196 716 966 4191 5722 6502
Size: 15 168 13 520 250 3225 1531 780
Cuts at: 6502 7398 8372 8485 8659 8785 8812 9230 9268
Size: 896 974 113 174 126 27 418 38
Cuts at: 9268 10111 10264 10595 10789 10992
Size: 843 153 331 194 203
Fragments arranged by size:
3225 1531 974 896 843 780 520 418
331 250 203 194 174 168 153 126
113 38 27 15 13
TatI w'GTAC_w
Cuts at: 0 7122 7192 9130 10992
Size: 7122 70 1938 1862
Fragments arranged by size:
7122 1938 1862 70
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1376 1408 1716 1748 8347 8379 8768 8800
Size: 1376 32 308 32 6599 32 389 32
Cuts at: 8800 9957 9989 10456 10488 10992
Size: 1157 32 467 32 504
Fragments arranged by size:
6599 1376 1157 504 467 389 308 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2719 8887 9261 10776 10992
Size: 2719 6168 374 1515 216
Fragments arranged by size:
6168 2719 1515 374 216
XbaI T'CTAG_A
Cuts at: 0 748 787 820 3070 4635 4938 10992
Size: 748 39 33 2250 1565 303 6054
Fragments arranged by size:
6054 2250 1565 748 303 39 33
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 5796 5936 6045 6255 6388 9168 10579 10992
Size: 5796 140 109 210 133 2780 1411 413
Fragments arranged by size:
5796 2780 1411 413 210 140 133 109
XhoI C'TCGA_G
Cuts at: 0 518 1955 2642 9500 10992
Size: 518 1437 687 6858 1492
Fragments arranged by size:
6858 1492 1437 687 518
XmaI C'CCGG_G
Cuts at: 0 674 5212 9091 10992
Size: 674 4538 3879 1901
Fragments arranged by size:
4538 3879 1901 674
XmnI GAAnn'nnTTC
Cuts at: 0 9347 10992
Size: 9347 1645
ZraI GAC'GTC
Cuts at: 0 2197 10992
Size: 2197 8795
Enzymes that do cut:
AarI AatII AccI AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I HincII HindIII KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NruI
NspI PasI PciI PflMI PfoI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AflII AhdI AscI AseI AsiSI BaeI BfrBI
BsiWI BsrGI BssHII BstZ17I EagI EciI FseI FspI
FspAI HpaI KpnI MfeI MluI NdeI NotI NsiI
PacI PmeI PmlI PpiI RsrII SalI SfiI SgfI
SnaBI SrfI SspI SwaI