Restriction map for
pFN21AE2584
(Linear) (Six-base) MAPSORT of: KIEE2584.seq Check: 1440 from: 1 to: 618
KIEE2584.orf
With 182 enzymes: SgfI *
August 7, 2015 15:48 ..
AccI GT'mk_AC
Cuts at: 0 80 618
Size: 80 538
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 618
Size: 404 214
AleI CACnn'nnGTG
Cuts at: 0 88 618
Size: 88 530
AlwNI CAG_nnn'CTG
Cuts at: 0 130 198 276 287 618
Size: 130 68 78 11 331
Fragments arranged by size:
331 130 78 68 11
ApoI r'AATT_y
Cuts at: 0 30 618
Size: 30 588
BamHI G'GATC_C
Cuts at: 0 603 618
Size: 603 15
BanII G_rGCy'C
Cuts at: 0 146 230 445 618
Size: 146 84 215 173
Fragments arranged by size:
215 173 146 84
BbsI GAAGACnn'nnnn_
Cuts at: 0 388 421 618
Size: 388 33 197
Fragments arranged by size:
388 197 33
BbvCI CC'TCA_GC
Cuts at: 0 131 573 618
Size: 131 442 45
Fragments arranged by size:
442 131 45
BlpI GC'TnA_GC
Cuts at: 0 152 618
Size: 152 466
BmgBI CAC'GTC
Cuts at: 0 297 618
Size: 297 321
BmrI ACTGGGnnnn_n'
Cuts at: 0 357 618
Size: 357 261
Bpu10I CC'TnA_GC
Cuts at: 0 131 145 573 618
Size: 131 14 428 45
Fragments arranged by size:
428 131 45 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 618
Size: 214 404
BsaI GGTCTCn'nnnn_
Cuts at: 0 309 618
Size: 309 309
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 151 154 249 618
Size: 148 3 3 95 369
Fragments arranged by size:
369 148 95 3 3
BseYI C'CCAG_C
Cuts at: 0 240 618
Size: 240 378
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 618
Size: 319 299
BsiHKAI G_wGCw'C
Cuts at: 0 146 230 618
Size: 146 84 388
Fragments arranged by size:
388 146 84
Bsp1286I G_dGCh'C
Cuts at: 0 146 230 445 618
Size: 146 84 215 173
Fragments arranged by size:
215 173 146 84
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 291 618
Size: 291 327
BsrFI r'CCGG_y
Cuts at: 0 10 618
Size: 10 608
BstAPI GCAn_nnn'nTGC
Cuts at: 0 297 618
Size: 297 321
BstYI r'GATC_y
Cuts at: 0 603 618
Size: 603 15
BtsI GCAGTG_nn'
Cuts at: 0 561 618
Size: 561 57
DrdI GACnn_nn'nnGTC
Cuts at: 0 301 492 522 618
Size: 301 191 30 96
Fragments arranged by size:
301 191 96 30
EaeI y'GGCC_r
Cuts at: 0 12 618
Size: 12 606
EarI CTCTTCn'nnn_
Cuts at: 0 164 618
Size: 164 454
EcoICRI GAG'CTC
Cuts at: 0 144 228 618
Size: 144 84 390
Fragments arranged by size:
390 144 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 618
Size: 404 214
EcoNI CCTnn'n_nnAGG
Cuts at: 0 41 618
Size: 41 577
EcoO109I rG'GnC_Cy
Cuts at: 0 195 618
Size: 195 423
EcoRI G'AATT_C
Cuts at: 0 30 618
Size: 30 588
EcoRV GAT'ATC
Cuts at: 0 21 618
Size: 21 597
HaeII r_GCGC'y
Cuts at: 0 314 618
Size: 314 304
HincII GTy'rAC
Cuts at: 0 81 618
Size: 81 537
HindIII A'AGCT_T
Cuts at: 0 460 618
Size: 460 158
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 581 618
Size: 581 37
MslI CAynn'nnrTG
Cuts at: 0 88 618
Size: 88 530
MspA1I CmG'CkG
Cuts at: 0 424 577 618
Size: 424 153 41
Fragments arranged by size:
424 153 41
NaeI GCC'GGC
Cuts at: 0 12 618
Size: 12 606
NgoMIV G'CCGG_C
Cuts at: 0 10 618
Size: 10 608
PasI CC'CwG_GG
Cuts at: 0 255 618
Size: 255 363
PflMI CCAn_nnn'nTGG
Cuts at: 0 198 287 618
Size: 198 89 331
Fragments arranged by size:
331 198 89
PfoI T'CCnGG_A
Cuts at: 0 280 618
Size: 280 338
PpuMI rG'GwC_Cy
Cuts at: 0 195 618
Size: 195 423
PvuII CAG'CTG
Cuts at: 0 424 618
Size: 424 194
SacI G_AGCT'C
Cuts at: 0 146 230 618
Size: 146 84 388
Fragments arranged by size:
388 146 84
SalI G'TCGA_C
Cuts at: 0 79 618
Size: 79 539
SmlI C'TyrA_G
Cuts at: 0 229 618
Size: 229 389
StyI C'CwwG_G
Cuts at: 0 161 206 618
Size: 161 45 412
Fragments arranged by size:
412 161 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 521 526 618
Size: 521 5 92
Fragments arranged by size:
521 92 5
Tth111I GACn'n_nGTC
Cuts at: 0 363 618
Size: 363 255
Enzymes that do cut:
AccI AcuI AleI AlwNI ApoI BamHI BanII BbsI
BbvCI BlpI BmgBI BmrI Bpu10I BpuEI BsaI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrFI BstAPI BstYI
BtsI DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV HaeII HincII HindIII MmeI MslI MspA1I
NaeI NgoMIV PasI PflMI PfoI PpuMI PvuII SacI
SalI SmlI StyI TaqII Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BanI BbeI BcgI BciVI BclI
BfrBI BglI BglII Bme1580I BmtI BplI BpmI BsaAI
BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraI DraIII EagI EciI FalI FseI FspI FspAI
Hin4I HpaI KasI KpnI MfeI MluI MscI NarI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
TstI XbaI XcmI XhoI XmaI XmnI ZraI