Restriction map for
pFN21AE2579
(Linear) (Six-base) MAPSORT of: KIEE2579.seq Check: 7897 from: 1 to: 1665
KIEE2579.orf
With 182 enzymes: SgfI *
June 25, 2015 11:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1473 1665
Size: 1473 192
AatII G_ACGT'C
Cuts at: 0 479 1665
Size: 479 1186
AccI GT'mk_AC
Cuts at: 0 932 1665
Size: 932 733
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1168 1665
Size: 1168 497
AflIII A'CryG_T
Cuts at: 0 291 852 1665
Size: 291 561 813
Fragments arranged by size:
813 561 291
AleI CACnn'nnGTG
Cuts at: 0 292 1665
Size: 292 1373
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1527 1665
Size: 1527 138
AlwNI CAG_nnn'CTG
Cuts at: 0 1488 1665
Size: 1488 177
ApoI r'AATT_y
Cuts at: 0 743 1665
Size: 743 922
BamHI G'GATC_C
Cuts at: 0 323 1665
Size: 323 1342
BanI G'GyrC_C
Cuts at: 0 559 1665
Size: 559 1106
BanII G_rGCy'C
Cuts at: 0 1040 1665
Size: 1040 625
BbsI GAAGACnn'nnnn_
Cuts at: 0 471 993 1665
Size: 471 522 672
Fragments arranged by size:
672 522 471
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 288 322 1665
Size: 288 34 1343
Fragments arranged by size:
1343 288 34
BclI T'GATC_A
Cuts at: 0 237 434 1665
Size: 237 197 1231
Fragments arranged by size:
1231 237 197
BglI GCCn_nnn'nGGC
Cuts at: 0 537 641 1665
Size: 537 104 1024
Fragments arranged by size:
1024 537 104
BglII A'GATC_T
Cuts at: 0 311 1665
Size: 311 1354
BlpI GC'TnA_GC
Cuts at: 0 115 1665
Size: 115 1550
Bme1580I G_kGCm'C
Cuts at: 0 562 589 1665
Size: 562 27 1076
Fragments arranged by size:
1076 562 27
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 1665
Size: 94 1571
Bpu10I CC'TnA_GC
Cuts at: 0 886 1665
Size: 886 779
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 360 1665
Size: 360 1305
BsaI GGTCTCn'nnnn_
Cuts at: 0 1165 1665
Size: 1165 500
BsaAI yAC'GTr
Cuts at: 0 294 855 1665
Size: 294 561 810
Fragments arranged by size:
810 561 294
BsaHI Gr'CG_yC
Cuts at: 0 476 800 1196 1665
Size: 476 324 396 469
Fragments arranged by size:
476 469 396 324
BsaWI w'CCGG_w
Cuts at: 0 192 1665
Size: 192 1473
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 171 201 1665
Size: 171 30 1464
Fragments arranged by size:
1464 171 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 847 1665
Size: 847 818
BseYI C'CCAG_C
Cuts at: 0 1278 1287 1488 1585 1665
Size: 1278 9 201 97 80
Fragments arranged by size:
1278 201 97 80 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 748 1501 1665
Size: 748 753 164
Fragments arranged by size:
753 748 164
BsiEI CG_ry'CG
Cuts at: 0 707 1665
Size: 707 958
BsiHKAI G_wGCw'C
Cuts at: 0 1040 1665
Size: 1040 625
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1203 1665
Size: 1203 462
Bsp1286I G_dGCh'C
Cuts at: 0 562 589 1040 1665
Size: 562 27 451 625
Fragments arranged by size:
625 562 451 27
BspHI T'CATG_A
Cuts at: 0 341 1665
Size: 341 1324
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 689 1473 1665
Size: 689 784 192
Fragments arranged by size:
784 689 192
BsrBI CCG'CTC
Cuts at: 0 187 993 1665
Size: 187 806 672
Fragments arranged by size:
806 672 187
BsrFI r'CCGG_y
Cuts at: 0 1210 1665
Size: 1210 455
BssSI C'ACGA_G
Cuts at: 0 58 155 1665
Size: 58 97 1510
Fragments arranged by size:
1510 97 58
BstBI TT'CG_AA
Cuts at: 0 138 1665
Size: 138 1527
BstEII G'GTnAC_C
Cuts at: 0 198 1665
Size: 198 1467
BstXI CCAn_nnnn'nTGG
Cuts at: 0 510 1665
Size: 510 1155
BstYI r'GATC_y
Cuts at: 0 41 311 323 1665
Size: 41 270 12 1342
Fragments arranged by size:
1342 270 41 12
Bsu36I CC'TnA_GG
Cuts at: 0 1074 1665
Size: 1074 591
BtgI C'CryG_G
Cuts at: 0 102 578 707 716 1202 1540 1665
Size: 102 476 129 9 486 338 125
Fragments arranged by size:
486 476 338 129 125 102 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 927 1251 1338 1365 1665
Size: 927 324 87 27 300
Fragments arranged by size:
927 324 300 87 27
BtsI GCAGTG_nn'
Cuts at: 0 532 1442 1665
Size: 532 910 223
Fragments arranged by size:
910 532 223
DraIII CAC_nnn'GTG
Cuts at: 0 593 1665
Size: 593 1072
EaeI y'GGCC_r
Cuts at: 0 704 1208 1665
Size: 704 504 457
Fragments arranged by size:
704 504 457
EagI C'GGCC_G
Cuts at: 0 704 1665
Size: 704 961
EarI CTCTTCn'nnn_
Cuts at: 0 493 691 1000 1045 1261 1665
Size: 493 198 309 45 216 404
Fragments arranged by size:
493 404 309 216 198 45
EcoICRI GAG'CTC
Cuts at: 0 1038 1665
Size: 1038 627
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 1168 1665
Size: 94 1074 497
Fragments arranged by size:
1074 497 94
EcoNI CCTnn'n_nnAGG
Cuts at: 0 436 829 1665
Size: 436 393 836
Fragments arranged by size:
836 436 393
EcoO109I rG'GnC_Cy
Cuts at: 0 429 1365 1507 1521 1665
Size: 429 936 142 14 144
Fragments arranged by size:
936 429 144 142 14
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1434 1665
Size: 1434 231
FspI TGC'GCA
Cuts at: 0 298 1665
Size: 298 1367
FspAI rTGC'GCAy
Cuts at: 0 298 1665
Size: 298 1367
HaeII r_GCGC'y
Cuts at: 0 1066 1665
Size: 1066 599
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1364 1396 1665
Size: 1364 32 269
Fragments arranged by size:
1364 269 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 1665
Size: 497 1168
MslI CAynn'nnrTG
Cuts at: 0 292 752 1665
Size: 292 460 913
Fragments arranged by size:
913 460 292
NaeI GCC'GGC
Cuts at: 0 1212 1665
Size: 1212 453
NcoI C'CATG_G
Cuts at: 0 102 1665
Size: 102 1563
NgoMIV G'CCGG_C
Cuts at: 0 1210 1665
Size: 1210 455
PasI CC'CwG_GG
Cuts at: 0 906 1665
Size: 906 759
PfoI T'CCnGG_A
Cuts at: 0 386 509 671 1403 1665
Size: 386 123 162 732 262
Fragments arranged by size:
732 386 262 162 123
PmlI CAC'GTG
Cuts at: 0 294 855 1665
Size: 294 561 810
Fragments arranged by size:
810 561 294
PpuMI rG'GwC_Cy
Cuts at: 0 1521 1665
Size: 1521 144
PshAI GACnn'nnGTC
Cuts at: 0 1237 1665
Size: 1237 428
PstI C_TGCA'G
Cuts at: 0 470 1665
Size: 470 1195
SacI G_AGCT'C
Cuts at: 0 1040 1665
Size: 1040 625
SanDI GG'GwC_CC
Cuts at: 0 1521 1665
Size: 1521 144
SapI GCTCTTCn'nnn_
Cuts at: 0 691 1000 1045 1261 1665
Size: 691 309 45 216 404
Fragments arranged by size:
691 404 309 216 45
SexAI A'CCwGG_T
Cuts at: 0 1297 1665
Size: 1297 368
SfcI C'TryA_G
Cuts at: 0 466 1008 1665
Size: 466 542 657
Fragments arranged by size:
657 542 466
SmlI C'TyrA_G
Cuts at: 0 375 1665
Size: 375 1290
StuI AGG'CCT
Cuts at: 0 262 1665
Size: 262 1403
StyI C'CwwG_G
Cuts at: 0 102 1337 1515 1614 1665
Size: 102 1235 178 99 51
Fragments arranged by size:
1235 178 102 99 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 171 203 446 478 1545 1577 1665
Size: 171 32 243 32 1067 32 88
Fragments arranged by size:
1067 243 171 88 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 802 1665
Size: 802 863
XmnI GAAnn'nnTTC
Cuts at: 0 601 1417 1665
Size: 601 816 248
Fragments arranged by size:
816 601 248
ZraI GAC'GTC
Cuts at: 0 477 1665
Size: 477 1188
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AleI AlfI AlwNI
ApoI BamHI BanI BanII BbsI BcgI BclI BglI
BglII BlpI Bme1580I BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BssSI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI FspAI HaeII Hin4I MmeI MslI NaeI NcoI
NgoMIV PasI PfoI PmlI PpuMI PshAI PstI SacI
SanDI SapI SexAI SfcI SmlI StuI StyI TstI
Tth111I XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AloI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbeI
BbvCI BciVI BfrBI BmgBI BmrI BmtI BplI BsaBI
BsiWI BsmI BspEI BsrDI BsrGI BssHII BstAPI BstZ17I
ClaI CspCI DraI DrdI EciI EcoRI EcoRV FseI
HincII HindIII HpaI KasI KpnI MfeI MluI MscI
MspA1I NarI NdeI NheI NotI NruI NsiI NspI
PacI PciI PflMI PmeI PpiI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacII SalI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TatI XbaI XcmI XhoI
XmaI