Restriction map for
pFN21AE2578
(Linear) (Six-base) MAPSORT of: KIEE2578.seq Check: 678 from: 1 to: 3504
KIEE2578.orf
With 182 enzymes: SgfI *
June 25, 2015 11:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 94 360 998 2349 2372 2434 2733 3271
Size: 94 266 638 1351 23 62 299 538
Cuts at: 3271 3504
Size: 233
Fragments arranged by size:
1351 638 538 299 266 233 94 62
23
AccI GT'mk_AC
Cuts at: 0 737 1355 2752 3504
Size: 737 618 1397 752
Fragments arranged by size:
1397 752 737 618
AflIII A'CryG_T
Cuts at: 0 1616 2279 3504
Size: 1616 663 1225
Fragments arranged by size:
1616 1225 663
AleI CACnn'nnGTG
Cuts at: 0 490 1337 3504
Size: 490 847 2167
Fragments arranged by size:
2167 847 490
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 884 1197 2131 3504
Size: 884 313 934 1373
Fragments arranged by size:
1373 934 884 313
AlwNI CAG_nnn'CTG
Cuts at: 0 1197 1569 3504
Size: 1197 372 1935
Fragments arranged by size:
1935 1197 372
ApaI G_GGCC'C
Cuts at: 0 178 3271 3496 3504
Size: 178 3093 225 8
Fragments arranged by size:
3093 225 178 8
ApaLI G'TGCA_C
Cuts at: 0 1182 1438 3504
Size: 1182 256 2066
Fragments arranged by size:
2066 1182 256
ApoI r'AATT_y
Cuts at: 0 2331 3504
Size: 2331 1173
AvaI C'yCGr_G
Cuts at: 0 98 1316 1550 1715 2885 3504
Size: 98 1218 234 165 1170 619
Fragments arranged by size:
1218 1170 619 234 165 98
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 269 302 1549 1582 3504
Size: 269 33 1247 33 1922
Fragments arranged by size:
1922 1247 269 33 33
BamHI G'GATC_C
Cuts at: 0 1995 3504
Size: 1995 1509
BanI G'GyrC_C
Cuts at: 0 86 185 318 330 356 719 868 1660
Size: 86 99 133 12 26 363 149 792
Cuts at: 1660 1837 2413 3504
Size: 177 576 1091
Fragments arranged by size:
1091 792 576 363 177 149 133 99
86 26 12
BanII G_rGCy'C
Cuts at: 0 178 581 1220 2540 3271 3297 3496 3504
Size: 178 403 639 1320 731 26 199 8
Fragments arranged by size:
1320 731 639 403 199 178 26 8
BbeI G_GCGC'C
Cuts at: 0 90 322 360 1664 3504
Size: 90 232 38 1304 1840
Fragments arranged by size:
1840 1304 232 90 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 1073 1094 2028 2478 3504
Size: 1073 21 934 450 1026
Fragments arranged by size:
1073 1026 934 450 21
BbvCI CC'TCA_GC
Cuts at: 0 311 527 784 3504
Size: 311 216 257 2720
Fragments arranged by size:
2720 311 257 216
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 985 1019 1696 1730 3504
Size: 985 34 677 34 1774
Fragments arranged by size:
1774 985 677 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 270 3504
Size: 270 3234
BclI T'GATC_A
Cuts at: 0 1911 3504
Size: 1911 1593
BglI GCCn_nnn'nGGC
Cuts at: 0 541 1532 1604 1696 1805 3504
Size: 541 991 72 92 109 1699
Fragments arranged by size:
1699 991 541 109 92 72
BglII A'GATC_T
Cuts at: 0 1106 1271 3504
Size: 1106 165 2233
Fragments arranged by size:
2233 1106 165
BlpI GC'TnA_GC
Cuts at: 0 2738 3504
Size: 2738 766
Bme1580I G_kGCm'C
Cuts at: 0 178 190 335 724 1186 1442 1840 2028
Size: 178 12 145 389 462 256 398 188
Cuts at: 2028 2220 2416 3271 3496 3504
Size: 192 196 855 225 8
Fragments arranged by size:
855 462 398 389 256 225 196 192
188 178 145 12 8
BmgBI CAC'GTC
Cuts at: 0 2865 3504
Size: 2865 639
BmrI ACTGGGnnnn_n'
Cuts at: 0 1173 3265 3504
Size: 1173 2092 239
Fragments arranged by size:
2092 1173 239
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1389 3504
Size: 1389 2115
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 3004 3448 3504
Size: 427 2577 444 56
Fragments arranged by size:
2577 444 427 56
Bpu10I CC'TnA_GC
Cuts at: 0 311 527 784 1882 3504
Size: 311 216 257 1098 1622
Fragments arranged by size:
1622 1098 311 257 216
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1609 1990 3504
Size: 1609 381 1514
Fragments arranged by size:
1609 1514 381
BsaI GGTCTCn'nnnn_
Cuts at: 0 952 2629 3504
Size: 952 1677 875
Fragments arranged by size:
1677 952 875
BsaAI yAC'GTr
Cuts at: 0 639 1384 3504
Size: 639 745 2120
Fragments arranged by size:
2120 745 639
BsaBI GATnn'nnATC
Cuts at: 0 1270 3504
Size: 1270 2234
BsaHI Gr'CG_yC
Cuts at: 0 87 203 319 357 1448 1661 2609 3504
Size: 87 116 116 38 1091 213 948 895
Fragments arranged by size:
1091 948 895 213 116 116 87 38
BsaWI w'CCGG_w
Cuts at: 0 212 2831 3504
Size: 212 2619 673
Fragments arranged by size:
2619 673 212
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 400 430 2000 2030 3504
Size: 400 30 1570 30 1474
Fragments arranged by size:
1570 1474 400 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 745 1696 2818 2827 3076 3504
Size: 745 951 1122 9 249 428
Fragments arranged by size:
1122 951 745 428 249 9
BseYI C'CCAG_C
Cuts at: 0 157 243 645 1040 1748 2562 2571 3496
Size: 157 86 402 395 708 814 9 925
Cuts at: 3496 3504
Size: 8
Fragments arranged by size:
925 814 708 402 395 157 86 9
8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 388 685 876 897 2336 2476 3504
Size: 99 289 297 191 21 1439 140 1028
Fragments arranged by size:
1439 1028 297 289 191 140 99 21
BsiEI CG_ry'CG
Cuts at: 0 3372 3504
Size: 3372 132
BsiHKAI G_wGCw'C
Cuts at: 0 1186 1220 1442 3227 3504
Size: 1186 34 222 1785 277
Fragments arranged by size:
1785 1186 277 222 34
Bsp1286I G_dGCh'C
Cuts at: 0 178 190 335 581 724 1186 1220 1442
Size: 178 12 145 246 143 462 34 222
Cuts at: 1442 1840 2028 2220 2416 2540 3227 3271 3297
Size: 398 188 192 196 124 687 44 26
Cuts at: 3297 3496 3504
Size: 199 8
Fragments arranged by size:
687 462 398 246 222 199 196 192
188 178 145 143 124 44 34 26
12 8
BspEI T'CCGG_A
Cuts at: 0 212 3504
Size: 212 3292
BspHI T'CATG_A
Cuts at: 0 1202 3504
Size: 1202 2302
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 94 360 657 998 2349 2372 2434 2733
Size: 94 266 297 341 1351 23 62 299
Cuts at: 2733 3271 3504
Size: 538 233
Fragments arranged by size:
1351 538 341 299 297 266 233 94
62 23
BsrBI CCG'CTC
Cuts at: 0 1017 2210 3066 3158 3504
Size: 1017 1193 856 92 346
Fragments arranged by size:
1193 1017 856 346 92
BsrDI GCAATG_nn'
Cuts at: 0 72 2270 2904 2951 3504
Size: 72 2198 634 47 553
Fragments arranged by size:
2198 634 553 72 47
BsrFI r'CCGG_y
Cuts at: 0 596 1937 3504
Size: 596 1341 1567
Fragments arranged by size:
1567 1341 596
BstEII G'GTnAC_C
Cuts at: 0 3381 3504
Size: 3381 123
BstXI CCAn_nnnn'nTGG
Cuts at: 0 627 1377 1512 1728 2865 3504
Size: 627 750 135 216 1137 639
Fragments arranged by size:
1137 750 639 627 216 135
BstYI r'GATC_y
Cuts at: 0 1106 1271 1965 1995 2096 3504
Size: 1106 165 694 30 101 1408
Fragments arranged by size:
1408 1106 694 165 101 30
Bsu36I CC'TnA_GG
Cuts at: 0 1261 1859 2174 3120 3504
Size: 1261 598 315 946 384
Fragments arranged by size:
1261 946 598 384 315
BtgI C'CryG_G
Cuts at: 0 90 837 963 1052 1599 1727 2613 3386
Size: 90 747 126 89 547 128 886 773
Cuts at: 3386 3504
Size: 118
Fragments arranged by size:
886 773 747 547 128 126 118 90
89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 726 3018 3504
Size: 726 2292 486
Fragments arranged by size:
2292 726 486
BtsI GCAGTG_nn'
Cuts at: 0 263 668 1184 1254 1332 1440 1558 2351
Size: 263 405 516 70 78 108 118 793
Cuts at: 2351 3167 3504
Size: 816 337
Fragments arranged by size:
816 793 516 405 337 263 118 108
78 70
ClaI AT'CG_AT
Cuts at: 0 1895 3504
Size: 1895 1609
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2565 2600 3504
Size: 2565 35 904
Fragments arranged by size:
2565 904 35
EaeI y'GGCC_r
Cuts at: 0 398 1502 1533 1697 2474 2516 3504
Size: 398 1104 31 164 777 42 988
Fragments arranged by size:
1104 988 777 398 164 42 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 598 1786 3504
Size: 598 1188 1718
Fragments arranged by size:
1718 1188 598
EcoICRI GAG'CTC
Cuts at: 0 1218 3504
Size: 1218 2286
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 3004 3448 3504
Size: 427 2577 444 56
Fragments arranged by size:
2577 444 427 56
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2407 3397 3504
Size: 2407 990 107
Fragments arranged by size:
2407 990 107
EcoO109I rG'GnC_Cy
Cuts at: 0 175 2178 2566 3363 3492 3493 3504
Size: 175 2003 388 797 129 1 11
Fragments arranged by size:
2003 797 388 175 129 11 1
FspI TGC'GCA
Cuts at: 0 1190 3504
Size: 1190 2314
HaeII r_GCGC'y
Cuts at: 0 90 322 360 414 680 945 1664 2725
Size: 90 232 38 54 266 265 719 1061
Cuts at: 2725 2895 3504
Size: 170 609
Fragments arranged by size:
1061 719 609 266 265 232 170 90
54 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1468 1500 1950 1982 3428 3460 3504
Size: 1468 32 450 32 1446 32 44
Fragments arranged by size:
1468 1446 450 44 32 32 32
HincII GTy'rAC
Cuts at: 0 2753 3504
Size: 2753 751
KasI G'GCGC_C
Cuts at: 0 86 318 356 1660 3504
Size: 86 232 38 1304 1844
Fragments arranged by size:
1844 1304 232 86 38
MluI A'CGCG_T
Cuts at: 0 1616 3504
Size: 1616 1888
MscI TGG'CCA
Cuts at: 0 1504 1699 2476 2518 3504
Size: 1504 195 777 42 986
Fragments arranged by size:
1504 986 777 195 42
MslI CAynn'nnrTG
Cuts at: 0 490 586 625 766 1057 1127 1179 1337
Size: 490 96 39 141 291 70 52 158
Cuts at: 1337 1414 2086 3109 3218 3504
Size: 77 672 1023 109 286
Fragments arranged by size:
1023 672 490 291 286 158 141 109
96 77 70 52 39
MspA1I CmG'CkG
Cuts at: 0 298 423 490 788 965 1430 1710 1917
Size: 298 125 67 298 177 465 280 207
Cuts at: 1917 2662 3078 3426 3504
Size: 745 416 348 78
Fragments arranged by size:
745 465 416 348 298 298 280 207
177 125 78 67
NarI GG'CG_CC
Cuts at: 0 87 319 357 1661 3504
Size: 87 232 38 1304 1843
Fragments arranged by size:
1843 1304 232 87 38
NcoI C'CATG_G
Cuts at: 0 1052 1727 2613 3504
Size: 1052 675 886 891
Fragments arranged by size:
1052 891 886 675
NspI r_CATG'y
Cuts at: 0 771 1413 2283 2460 2737 3504
Size: 771 642 870 177 277 767
Fragments arranged by size:
870 771 767 642 277 177
PasI CC'CwG_GG
Cuts at: 0 264 3504
Size: 264 3240
PciI A'CATG_T
Cuts at: 0 2279 3504
Size: 2279 1225
PflMI CCAn_nnn'nTGG
Cuts at: 0 1600 2619 2880 3504
Size: 1600 1019 261 624
Fragments arranged by size:
1600 1019 624 261
PfoI T'CCnGG_A
Cuts at: 0 140 1771 1984 3504
Size: 140 1631 213 1520
Fragments arranged by size:
1631 1520 213 140
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3052 3084 3504
Size: 3052 32 420
Fragments arranged by size:
3052 420 32
PpuMI rG'GwC_Cy
Cuts at: 0 2566 3363 3504
Size: 2566 797 141
Fragments arranged by size:
2566 797 141
PspOMI G'GGCC_C
Cuts at: 0 174 3267 3492 3504
Size: 174 3093 225 12
Fragments arranged by size:
3093 225 174 12
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1564 1596 3504
Size: 1564 32 1908
Fragments arranged by size:
1908 1564 32
PstI C_TGCA'G
Cuts at: 0 995 1565 1709 2369 2431 2667 3504
Size: 995 570 144 660 62 236 837
Fragments arranged by size:
995 837 660 570 236 144 62
PvuII CAG'CTG
Cuts at: 0 1917 2662 3078 3426 3504
Size: 1917 745 416 348 78
Fragments arranged by size:
1917 745 416 348 78
SacI G_AGCT'C
Cuts at: 0 1220 3504
Size: 1220 2284
SacII CC_GC'GG
Cuts at: 0 966 3504
Size: 966 2538
SalI G'TCGA_C
Cuts at: 0 2751 3504
Size: 2751 753
SanDI GG'GwC_CC
Cuts at: 0 2566 3363 3504
Size: 2566 797 141
Fragments arranged by size:
2566 797 141
ScaI AGT'ACT
Cuts at: 0 1981 2443 3504
Size: 1981 462 1061
Fragments arranged by size:
1981 1061 462
SexAI A'CCwGG_T
Cuts at: 0 3398 3504
Size: 3398 106
SfcI C'TryA_G
Cuts at: 0 991 1561 1705 2365 2427 2663 2782 2818
Size: 991 570 144 660 62 236 119 36
Cuts at: 2818 3504
Size: 686
Fragments arranged by size:
991 686 660 570 236 144 119 62
36
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1532 1696 3504
Size: 1532 164 1808
Fragments arranged by size:
1808 1532 164
SfoI GGC'GCC
Cuts at: 0 88 320 358 1662 3504
Size: 88 232 38 1304 1842
Fragments arranged by size:
1842 1304 232 88 38
SmaI CCC'GGG
Cuts at: 0 1318 2887 3504
Size: 1318 1569 617
Fragments arranged by size:
1569 1318 617
SmlI C'TyrA_G
Cuts at: 0 1624 1969 3504
Size: 1624 345 1535
Fragments arranged by size:
1624 1535 345
SphI G_CATG'C
Cuts at: 0 771 2737 3504
Size: 771 1966 767
Fragments arranged by size:
1966 771 767
SrfI GCCC'GGGC
Cuts at: 0 1318 3504
Size: 1318 2186
StuI AGG'CCT
Cuts at: 0 949 1792 3504
Size: 949 843 1712
Fragments arranged by size:
1712 949 843
StyI C'CwwG_G
Cuts at: 0 1052 1452 1727 1778 1787 2613 2858 3413
Size: 1052 400 275 51 9 826 245 555
Cuts at: 3413 3504
Size: 91
Fragments arranged by size:
1052 826 555 400 275 245 91 51
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 171 615 1855 3386 3504
Size: 171 444 1240 1531 118
Fragments arranged by size:
1531 1240 444 171 118
TatI w'GTAC_w
Cuts at: 0 1979 2150 2441 3504
Size: 1979 171 291 1063
Fragments arranged by size:
1979 1063 291 171
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 607 639 906 938 3504
Size: 607 32 267 32 2566
Fragments arranged by size:
2566 607 267 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1129 3504
Size: 1129 2375
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3195 3250 3504
Size: 3195 55 254
Fragments arranged by size:
3195 254 55
XmaI C'CCGG_G
Cuts at: 0 1316 2885 3504
Size: 1316 1569 619
Fragments arranged by size:
1569 1316 619
XmnI GAAnn'nnTTC
Cuts at: 0 388 3504
Size: 388 3116
Enzymes that do cut:
AarI AccI AflIII AleI AlfI AlwNI ApaI ApaLI
ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I HincII KasI MluI MscI MslI MspA1I
NarI NcoI NspI PasI PciI PflMI PfoI PpiI
PpuMI PspOMI PsrI PstI PvuII SacI SacII SalI
SanDI ScaI SexAI SfcI SfiI SfoI SmaI SmlI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AloI AscI AseI AsiSI AvrII BfrBI BmtI BsiWI
BsmI BsmBI BsrGI BssHII BssSI BstAPI BstBI BstZ17I
DraI DraIII DrdI EagI EarI EcoRI EcoRV FalI
FseI FspAI HindIII HpaI KpnI MfeI MmeI NaeI
NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI
PmlI PshAI PsiI PspXI PvuI RsrII SapI SbfI
SgfI SgrAI SnaBI SpeI SspI SwaI XbaI XhoI
ZraI