Restriction map for
pFN21AE2568
(Linear) (Six-base) MAPSORT of: KIEE2568.seq Check: 3302 from: 1 to: 795
KIEE2568.orf
With 182 enzymes: SgfI *
March 23, 2015 09:39 ..
AleI CACnn'nnGTG
Cuts at: 0 586 795
Size: 586 209
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 205 237 795
Size: 205 32 558
Fragments arranged by size:
558 205 32
AlwNI CAG_nnn'CTG
Cuts at: 0 449 795
Size: 449 346
ApaI G_GGCC'C
Cuts at: 0 595 795
Size: 595 200
ApaLI G'TGCA_C
Cuts at: 0 64 119 795
Size: 64 55 676
Fragments arranged by size:
676 64 55
ApoI r'AATT_y
Cuts at: 0 729 795
Size: 729 66
AvaI C'yCGr_G
Cuts at: 0 595 795
Size: 595 200
BanI G'GyrC_C
Cuts at: 0 347 695 795
Size: 347 348 100
Fragments arranged by size:
348 347 100
BanII G_rGCy'C
Cuts at: 0 595 795
Size: 595 200
BbsI GAAGACnn'nnnn_
Cuts at: 0 375 795
Size: 375 420
BbvCI CC'TCA_GC
Cuts at: 0 428 795
Size: 428 367
BciVI GTATCCnnnnn_n'
Cuts at: 0 250 795
Size: 250 545
BglII A'GATC_T
Cuts at: 0 287 795
Size: 287 508
BlpI GC'TnA_GC
Cuts at: 0 184 795
Size: 184 611
Bme1580I G_kGCm'C
Cuts at: 0 68 123 204 437 595 698 795
Size: 68 55 81 233 158 103 97
Fragments arranged by size:
233 158 103 97 81 68 55
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 475 781 795
Size: 119 356 306 14
Fragments arranged by size:
356 306 119 14
Bpu10I CC'TnA_GC
Cuts at: 0 18 428 795
Size: 18 410 367
Fragments arranged by size:
410 367 18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 205 235 512 542 795
Size: 205 30 277 30 253
Fragments arranged by size:
277 253 205 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 223 391 795
Size: 223 168 404
Fragments arranged by size:
404 223 168
BseYI C'CCAG_C
Cuts at: 0 143 338 536 738 795
Size: 143 195 198 202 57
Fragments arranged by size:
202 198 195 143 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 694 795
Size: 172 522 101
Fragments arranged by size:
522 172 101
BsiHKAI G_wGCw'C
Cuts at: 0 68 123 795
Size: 68 55 672
Fragments arranged by size:
672 68 55
BsmBI CGTCTCn'nnnn_
Cuts at: 0 151 781 795
Size: 151 630 14
Fragments arranged by size:
630 151 14
Bsp1286I G_dGCh'C
Cuts at: 0 68 123 204 437 595 698 795
Size: 68 55 81 233 158 103 97
Fragments arranged by size:
233 158 103 97 81 68 55
BsrBI CCG'CTC
Cuts at: 0 183 795
Size: 183 612
BsrFI r'CCGG_y
Cuts at: 0 60 548 795
Size: 60 488 247
Fragments arranged by size:
488 247 60
BssSI C'ACGA_G
Cuts at: 0 205 795
Size: 205 590
BstYI r'GATC_y
Cuts at: 0 287 795
Size: 287 508
BtgI C'CryG_G
Cuts at: 0 75 795
Size: 75 720
BtsI GCAGTG_nn'
Cuts at: 0 49 66 679 795
Size: 49 17 613 116
Fragments arranged by size:
613 116 49 17
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 475 781 795
Size: 119 356 306 14
Fragments arranged by size:
356 306 119 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 763 795
Size: 763 32
EcoO109I rG'GnC_Cy
Cuts at: 0 592 795
Size: 592 203
EcoRI G'AATT_C
Cuts at: 0 729 795
Size: 729 66
EcoRV GAT'ATC
Cuts at: 0 579 795
Size: 579 216
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 462 795
Size: 462 333
HaeII r_GCGC'y
Cuts at: 0 90 492 795
Size: 90 402 303
Fragments arranged by size:
402 303 90
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 279 311 795
Size: 279 32 484
Fragments arranged by size:
484 279 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 795
Size: 683 112
MslI CAynn'nnrTG
Cuts at: 0 586 795
Size: 586 209
MspA1I CmG'CkG
Cuts at: 0 192 486 680 795
Size: 192 294 194 115
Fragments arranged by size:
294 194 192 115
NspI r_CATG'y
Cuts at: 0 500 795
Size: 500 295
PflMI CCAn_nnn'nTGG
Cuts at: 0 325 573 795
Size: 325 248 222
Fragments arranged by size:
325 248 222
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 205 237 761 793 795
Size: 205 32 524 32 2
Fragments arranged by size:
524 205 32 32 2
PshAI GACnn'nnGTC
Cuts at: 0 314 795
Size: 314 481
PspOMI G'GGCC_C
Cuts at: 0 591 795
Size: 591 204
PstI C_TGCA'G
Cuts at: 0 41 485 674 795
Size: 41 444 189 121
Fragments arranged by size:
444 189 121 41
PvuII CAG'CTG
Cuts at: 0 192 795
Size: 192 603
SexAI A'CCwGG_T
Cuts at: 0 128 795
Size: 128 667
SfcI C'TryA_G
Cuts at: 0 37 481 527 670 795
Size: 37 444 46 143 125
Fragments arranged by size:
444 143 125 46 37
SmaI CCC'GGG
Cuts at: 0 597 795
Size: 597 198
StyI C'CwwG_G
Cuts at: 0 419 795
Size: 419 376
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 403 561 795
Size: 403 158 234
Fragments arranged by size:
403 234 158
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 450 795
Size: 450 345
XmaI C'CCGG_G
Cuts at: 0 595 795
Size: 595 200
Enzymes that do cut:
AleI AloI AlwNI ApaI ApaLI ApoI AvaI BanI
BanII BbsI BbvCI BciVI BglII BlpI Bme1580I BpmI
Bpu10I BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BsrBI BsrFI BssSI BstYI BtgI BtsI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I MmeI MslI
MspA1I NspI PflMI PpiI PshAI PspOMI PstI PvuII
SexAI SfcI SmaI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BclI BfrBI BglI BmgBI
BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrDI
BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PmlI PpuMI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI TstI
Tth111I XbaI XhoI XmnI ZraI