(Linear) (Six-base) MAPSORT of: KIEE2562.seq  Check: 7940  from: 1  to: 795



KIEE2562.orf

 With 182 enzymes: SgfI * 

                        February 23, 2015 11:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     578     795 
   Size:        578     217
 
AflII C'TTAA_G

Cuts at:      0     297     405     795 
   Size:        297     108     390

  Fragments arranged by size: 

                 390     297     108
 
ApoI r'AATT_y

Cuts at:      0     712     795 
   Size:        712      83
 
BanII G_rGCy'C

Cuts at:      0      31     795 
   Size:         31     764
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     653     795 
   Size:        653     142
 
BglII A'GATC_T

Cuts at:      0     768     795 
   Size:        768      27
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     455     795 
   Size:        455     340
 
BsaWI w'CCGG_w

Cuts at:      0     740     795 
   Size:        740      55
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     400     403     795 
   Size:        400       3     392

  Fragments arranged by size: 

                 400     392       3
 
BsiHKAI G_wGCw'C

Cuts at:      0     119     795 
   Size:        119     676
 
Bsp1286I G_dGCh'C

Cuts at:      0      31     119     795 
   Size:         31      88     676

  Fragments arranged by size: 

                 676      88      31
 
BspEI T'CCGG_A

Cuts at:      0     740     795 
   Size:        740      55
 
BsrDI GCAATG_nn'

Cuts at:      0     197     795 
   Size:        197     598
 
BsrGI T'GTAC_A

Cuts at:      0     246     795 
   Size:        246     549
 
BstYI r'GATC_y

Cuts at:      0     768     795 
   Size:        768      27
 
Bsu36I CC'TnA_GG

Cuts at:      0      34     795 
   Size:         34     761
 
BtsI GCAGTG_nn'

Cuts at:      0     784     795 
   Size:        784      11
 
EarI CTCTTCn'nnn_

Cuts at:      0     377     500     698     795 
   Size:        377     123     198      97

  Fragments arranged by size: 

                 377     198     123      97
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     455     578     795 
   Size:        455     123     217

  Fragments arranged by size: 

                 455     217     123
 
EcoO109I rG'GnC_Cy

Cuts at:      0      38     631     795 
   Size:         38     593     164

  Fragments arranged by size: 

                 593     164      38
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     487     753     795 
   Size:        487     266      42

  Fragments arranged by size: 

                 487     266      42
 
HaeII r_GCGC'y

Cuts at:      0     571     795 
   Size:        571     224
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     355     387     795 
   Size:        355      32     408

  Fragments arranged by size: 

                 408     355      32
 
MslI CAynn'nnrTG

Cuts at:      0      22     332     795 
   Size:         22     310     463

  Fragments arranged by size: 

                 463     310      22
 
PpuMI rG'GwC_Cy

Cuts at:      0     631     795 
   Size:        631     164
 
SfcI C'TryA_G

Cuts at:      0     641     795 
   Size:        641     154
 
SmlI C'TyrA_G

Cuts at:      0     297     405     795 
   Size:        297     108     390

  Fragments arranged by size: 

                 390     297     108
 
StyI C'CwwG_G

Cuts at:      0     264     795 
   Size:        264     531
 
TatI w'GTAC_w

Cuts at:      0     246     795 
   Size:        246     549
 
XmnI GAAnn'nnTTC

Cuts at:      0     736     795 
   Size:        736      59
 

 Enzymes that do cut:

     AcuI    AflII     ApoI    BanII     BbsI    BglII     BpmI    BsaWI
    BseRI  BsiHKAI Bsp1286I    BspEI    BsrDI    BsrGI    BstYI   Bsu36I
     BtsI     EarI  Eco57MI EcoO109I     FalI    HaeII    Hin4I     MslI
    PpuMI     SfcI     SmlI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspHI    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI