Restriction map for
pFN21AE2557
(Linear) (Six-base) MAPSORT of: KIEE2557.seq Check: 5207 from: 1 to: 2841
KIEE2557.orf
With 182 enzymes: SgfI *
December 15, 2014 12:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1440 2841
Size: 1440 1401
AatII G_ACGT'C
Cuts at: 0 1274 2841
Size: 1274 1567
AccI GT'mk_AC
Cuts at: 0 2662 2841
Size: 2662 179
Acc65I G'GTAC_C
Cuts at: 0 1593 2841
Size: 1593 1248
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 601 739 844 2243 2272 2841
Size: 45 556 138 105 1399 29 569
Fragments arranged by size:
1399 569 556 138 105 45 29
AflII C'TTAA_G
Cuts at: 0 2527 2841
Size: 2527 314
AhdI GACnn_n'nnGTC
Cuts at: 0 912 2841
Size: 912 1929
AleI CACnn'nnGTG
Cuts at: 0 1432 2841
Size: 1432 1409
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 295 2108 2841
Size: 295 1813 733
Fragments arranged by size:
1813 733 295
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 413 445 2841
Size: 413 32 2396
Fragments arranged by size:
2396 413 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1191 1510 2841
Size: 1191 319 1331
Fragments arranged by size:
1331 1191 319
ApaI G_GGCC'C
Cuts at: 0 224 406 1981 2756 2841
Size: 224 182 1575 775 85
Fragments arranged by size:
1575 775 224 182 85
ApaLI G'TGCA_C
Cuts at: 0 1081 2841
Size: 1081 1760
ApoI r'AATT_y
Cuts at: 0 2431 2709 2841
Size: 2431 278 132
Fragments arranged by size:
2431 278 132
AvaI C'yCGr_G
Cuts at: 0 47 233 1265 2561 2841
Size: 47 186 1032 1296 280
Fragments arranged by size:
1296 1032 280 186 47
BanI G'GyrC_C
Cuts at: 0 918 1260 1369 1411 1593 2841
Size: 918 342 109 42 182 1248
Fragments arranged by size:
1248 918 342 182 109 42
BanII G_rGCy'C
Cuts at: 0 224 406 1981 2302 2756 2840 2841
Size: 224 182 1575 321 454 84 1
Fragments arranged by size:
1575 454 321 224 182 84 1
BbsI GAAGACnn'nnnn_
Cuts at: 0 383 2841
Size: 383 2458
BciVI GTATCCnnnnn_n'
Cuts at: 0 1921 2841
Size: 1921 920
BclI T'GATC_A
Cuts at: 0 1740 2841
Size: 1740 1101
BglI GCCn_nnn'nGGC
Cuts at: 0 1985 2841
Size: 1985 856
BglII A'GATC_T
Cuts at: 0 158 2841
Size: 158 2683
BlpI GC'TnA_GC
Cuts at: 0 1111 2734 2841
Size: 1111 1623 107
Fragments arranged by size:
1623 1111 107
Bme1580I G_kGCm'C
Cuts at: 0 224 406 923 1085 1212 1372 1981 2756
Size: 224 182 517 162 127 160 609 775
Cuts at: 2756 2841
Size: 85
Fragments arranged by size:
775 609 517 224 182 162 160 127
85
BmgBI CAC'GTC
Cuts at: 0 84 2841
Size: 84 2757
BmrI ACTGGGnnnn_n'
Cuts at: 0 306 351 409 1632 1722 1933 1992 2841
Size: 306 45 58 1223 90 211 59 849
Fragments arranged by size:
1223 849 306 211 90 59 58 45
BmtI G_CTAG'C
Cuts at: 0 154 2841
Size: 154 2687
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 832 889 931 988 998 1234 1342
Size: 706 126 57 42 57 10 236 108
Cuts at: 1342 1391 1960 2656 2841
Size: 49 569 696 185
Fragments arranged by size:
706 696 569 236 185 126 108 57
57 49 42 10
Bpu10I CC'TnA_GC
Cuts at: 0 671 1132 2296 2841
Size: 671 461 1164 545
Fragments arranged by size:
1164 671 545 461
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1285 2841
Size: 1285 1556
BsaI GGTCTCn'nnnn_
Cuts at: 0 651 1725 1862 2036 2841
Size: 651 1074 137 174 805
Fragments arranged by size:
1074 805 651 174 137
BsaHI Gr'CG_yC
Cuts at: 0 1271 2315 2841
Size: 1271 1044 526
Fragments arranged by size:
1271 1044 526
BsaWI w'CCGG_w
Cuts at: 0 616 1313 2330 2841
Size: 616 697 1017 511
Fragments arranged by size:
1017 697 616 511
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2809 2839 2841
Size: 2809 30 2
Fragments arranged by size:
2809 30 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 117 2262 2841
Size: 117 2145 579
Fragments arranged by size:
2145 579 117
BseYI C'CCAG_C
Cuts at: 0 74 114 462 486 702 806 1573 1881
Size: 74 40 348 24 216 104 767 308
Cuts at: 1881 2126 2841
Size: 245 715
Fragments arranged by size:
767 715 348 308 245 216 104 74
40 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 557 847 2515 2841
Size: 557 290 1668 326
Fragments arranged by size:
1668 557 326 290
BsiEI CG_ry'CG
Cuts at: 0 72 1314 2841
Size: 72 1242 1527
Fragments arranged by size:
1527 1242 72
BsiHKAI G_wGCw'C
Cuts at: 0 476 1085 2840 2841
Size: 476 609 1755 1
Fragments arranged by size:
1755 609 476 1
BsmI GAATG_Cn'
Cuts at: 0 418 2841
Size: 418 2423
Bsp1286I G_dGCh'C
Cuts at: 0 224 406 476 923 1085 1212 1372 1981
Size: 224 182 70 447 162 127 160 609
Cuts at: 1981 2302 2756 2840 2841
Size: 321 454 84 1
Fragments arranged by size:
609 454 447 321 224 182 162 160
127 84 70 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1440 1703 2702 2841
Size: 1440 263 999 139
Fragments arranged by size:
1440 999 263 139
BsrBI CCG'CTC
Cuts at: 0 1944 2784 2841
Size: 1944 840 57
Fragments arranged by size:
1944 840 57
BsrDI GCAATG_nn'
Cuts at: 0 530 2841
Size: 530 2311
BsrFI r'CCGG_y
Cuts at: 0 517 1414 1708 2841
Size: 517 897 294 1133
Fragments arranged by size:
1133 897 517 294
BsrGI T'GTAC_A
Cuts at: 0 1967 2841
Size: 1967 874
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1857 2841
Size: 1857 984
BstEII G'GTnAC_C
Cuts at: 0 630 2841
Size: 630 2211
BstXI CCAn_nnnn'nTGG
Cuts at: 0 450 1918 2046 2841
Size: 450 1468 128 795
Fragments arranged by size:
1468 795 450 128
BstYI r'GATC_y
Cuts at: 0 158 2502 2841
Size: 158 2344 339
Fragments arranged by size:
2344 339 158
BstZ17I GTA'TAC
Cuts at: 0 2663 2841
Size: 2663 178
Bsu36I CC'TnA_GG
Cuts at: 0 289 730 782 1598 2748 2841
Size: 289 441 52 816 1150 93
Fragments arranged by size:
1150 816 441 289 93 52
BtsI GCAGTG_nn'
Cuts at: 0 145 773 1703 1776 1933 2841
Size: 145 628 930 73 157 908
Fragments arranged by size:
930 908 628 157 145 73
ClaI AT'CG_AT
Cuts at: 0 1763 2841
Size: 1763 1078
DraIII CAC_nnn'GTG
Cuts at: 0 1089 2841
Size: 1089 1752
EaeI y'GGCC_r
Cuts at: 0 1773 2841
Size: 1773 1068
EarI CTCTTCn'nnn_
Cuts at: 0 340 2219 2841
Size: 340 1879 622
Fragments arranged by size:
1879 622 340
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 244 320 2841
Size: 244 76 2521
Fragments arranged by size:
2521 244 76
EcoICRI GAG'CTC
Cuts at: 0 2838 2841
Size: 2838 3
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 601 706 739 832 844 889 931
Size: 45 556 105 33 93 12 45 42
Cuts at: 931 988 998 1234 1342 1391 1960 2243 2272
Size: 57 10 236 108 49 569 283 29
Cuts at: 2272 2656 2841
Size: 384 185
Fragments arranged by size:
569 556 384 283 236 185 108 105
93 57 49 45 45 42 33 29
12 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2698 2776 2841
Size: 2698 78 65
Fragments arranged by size:
2698 78 65
EcoO109I rG'GnC_Cy
Cuts at: 0 220 221 403 490 933 1056 1885 1977
Size: 220 1 182 87 443 123 829 92
Cuts at: 1977 1978 2571 2752 2753 2799 2841
Size: 1 593 181 1 46 42
Fragments arranged by size:
829 593 443 220 182 181 123 92
87 46 42 1 1 1
EcoRI G'AATT_C
Cuts at: 0 2431 2841
Size: 2431 410
EcoRV GAT'ATC
Cuts at: 0 126 894 2841
Size: 126 768 1947
Fragments arranged by size:
1947 768 126
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 462 1131 1185 2841
Size: 462 669 54 1656
Fragments arranged by size:
1656 669 462 54
HaeII r_GCGC'y
Cuts at: 0 703 1230 2841
Size: 703 527 1611
Fragments arranged by size:
1611 703 527
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 9 41 898 930 2841
Size: 9 32 857 32 1911
Fragments arranged by size:
1911 857 32 32 9
KpnI G_GTAC'C
Cuts at: 0 1597 2841
Size: 1597 1244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1523 2841
Size: 1523 1318
MscI TGG'CCA
Cuts at: 0 1775 2841
Size: 1775 1066
MslI CAynn'nnrTG
Cuts at: 0 1432 1588 2584 2762 2841
Size: 1432 156 996 178 79
Fragments arranged by size:
1432 996 178 156 79
MspA1I CmG'CkG
Cuts at: 0 74 579 738 810 1191 1278 1341 1854
Size: 74 505 159 72 381 87 63 513
Cuts at: 1854 1881 2126 2499 2841
Size: 27 245 373 342
Fragments arranged by size:
513 505 381 373 342 245 159 87
74 72 63 27
NaeI GCC'GGC
Cuts at: 0 519 1710 2841
Size: 519 1191 1131
Fragments arranged by size:
1191 1131 519
NgoMIV G'CCGG_C
Cuts at: 0 517 1708 2841
Size: 517 1191 1133
Fragments arranged by size:
1191 1133 517
NheI G'CTAG_C
Cuts at: 0 150 2841
Size: 150 2691
PflMI CCAn_nnn'nTGG
Cuts at: 0 929 1482 1782 2841
Size: 929 553 300 1059
Fragments arranged by size:
1059 929 553 300
PfoI T'CCnGG_A
Cuts at: 0 975 1842 2045 2633 2841
Size: 975 867 203 588 208
Fragments arranged by size:
975 867 588 208 203
PpuMI rG'GwC_Cy
Cuts at: 0 490 933 1056 2841
Size: 490 443 123 1785
Fragments arranged by size:
1785 490 443 123
PshAI GACnn'nnGTC
Cuts at: 0 49 1316 2841
Size: 49 1267 1525
Fragments arranged by size:
1525 1267 49
PspOMI G'GGCC_C
Cuts at: 0 220 402 1977 2752 2841
Size: 220 182 1575 775 89
Fragments arranged by size:
1575 775 220 182 89
PstI C_TGCA'G
Cuts at: 0 881 1346 1673 2087 2841
Size: 881 465 327 414 754
Fragments arranged by size:
881 754 465 414 327
PvuII CAG'CTG
Cuts at: 0 579 738 810 1341 1854 1881 2499 2841
Size: 579 159 72 531 513 27 618 342
Fragments arranged by size:
618 579 531 513 342 159 72 27
SacI G_AGCT'C
Cuts at: 0 2840 2841
Size: 2840 1
SanDI GG'GwC_CC
Cuts at: 0 933 2841
Size: 933 1908
SfcI C'TryA_G
Cuts at: 0 877 1342 1669 1805 2025 2083 2841
Size: 877 465 327 136 220 58 758
Fragments arranged by size:
877 758 465 327 220 136 58
SmaI CCC'GGG
Cuts at: 0 2563 2841
Size: 2563 278
SmlI C'TyrA_G
Cuts at: 0 1300 2527 2841
Size: 1300 1227 314
Fragments arranged by size:
1300 1227 314
StuI AGG'CCT
Cuts at: 0 2089 2841
Size: 2089 752
StyI C'CwwG_G
Cuts at: 0 2436 2567 2841
Size: 2436 131 274
Fragments arranged by size:
2436 274 131
TatI w'GTAC_w
Cuts at: 0 1967 2841
Size: 1967 874
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 183 215 611 643 1362 1394 2809 2841
Size: 183 32 396 32 719 32 1415 32
Fragments arranged by size:
1415 719 396 183 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1102 2841
Size: 1102 1739
XmaI C'CCGG_G
Cuts at: 0 2561 2841
Size: 2561 280
XmnI GAAnn'nnTTC
Cuts at: 0 2629 2841
Size: 2629 212
ZraI GAC'GTC
Cuts at: 0 1272 2841
Size: 1272 1569
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BanI
BanII BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII
BstXI BstYI BstZ17I Bsu36I BtsI ClaI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I KpnI MmeI MscI MslI MspA1I
NaeI NgoMIV NheI PflMI PfoI PpuMI PshAI PspOMI
PstI PvuII SacI SanDI SfcI SmaI SmlI StuI
StyI TatI TstI Tth111I XmaI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflIII AgeI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BfrBI BplI BsaAI
BsaBI BsiWI BsmBI BspEI BspHI BssHII BssSI BstBI
BtgI BtgZI CspCI DraI DrdI EagI FseI FspI
FspAI HincII HindIII HpaI KasI MfeI MluI NarI
NcoI NdeI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PsiI PspXI PsrI PvuI
RsrII SacII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI TaqII XbaI XcmI XhoI