Restriction map for
pFN21AE2550
(Linear) (Six-base) MAPSORT of: KIEE2550.seq Check: 5203 from: 1 to: 1149
KIEE2550.orf
With 182 enzymes: SgfI *
October 31, 2014 16:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 850 1149
Size: 850 299
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 892 1149
Size: 892 257
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 820 852 1149
Size: 820 32 297
Fragments arranged by size:
820 297 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1044 1107 1149
Size: 1044 63 42
Fragments arranged by size:
1044 63 42
ApaI G_GGCC'C
Cuts at: 0 13 1149
Size: 13 1136
ApoI r'AATT_y
Cuts at: 0 498 1149
Size: 498 651
BanI G'GyrC_C
Cuts at: 0 1144 1149
Size: 1144 5
BanII G_rGCy'C
Cuts at: 0 13 74 388 416 474 1028 1149
Size: 13 61 314 28 58 554 121
Fragments arranged by size:
554 314 121 61 58 28 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 9 73 374 529 1149
Size: 9 64 301 155 620
Fragments arranged by size:
620 301 155 64 9
BfrBI ATG'CAT
Cuts at: 0 549 1149
Size: 549 600
BglII A'GATC_T
Cuts at: 0 1116 1149
Size: 1116 33
BlpI GC'TnA_GC
Cuts at: 0 68 1149
Size: 68 1081
Bme1580I G_kGCm'C
Cuts at: 0 13 617 887 1147 1149
Size: 13 604 270 260 2
Fragments arranged by size:
604 270 260 13 2
BmrI ACTGGGnnnn_n'
Cuts at: 0 453 1051 1149
Size: 453 598 98
Fragments arranged by size:
598 453 98
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 450 454 1149
Size: 322 128 4 695
Fragments arranged by size:
695 322 128 4
BsaI GGTCTCn'nnnn_
Cuts at: 0 247 1149
Size: 247 902
BsaHI Gr'CG_yC
Cuts at: 0 717 1149
Size: 717 432
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 820 850 1149
Size: 820 30 299
Fragments arranged by size:
820 299 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 618 841 1149
Size: 618 223 308
Fragments arranged by size:
618 308 223
BseYI C'CCAG_C
Cuts at: 0 6 389 749 1149
Size: 6 383 360 400
Fragments arranged by size:
400 383 360 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 1149
Size: 142 1007
BsiHKAI G_wGCw'C
Cuts at: 0 388 416 474 1028 1149
Size: 388 28 58 554 121
Fragments arranged by size:
554 388 121 58 28
BsmI GAATG_Cn'
Cuts at: 0 330 1149
Size: 330 819
Bsp1286I G_dGCh'C
Cuts at: 0 13 74 388 416 474 617 887 1028
Size: 13 61 314 28 58 143 270 141
Cuts at: 1028 1147 1149
Size: 119 2
Fragments arranged by size:
314 270 143 141 119 61 58 28
13 2
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 850 1029 1149
Size: 850 179 120
Fragments arranged by size:
850 179 120
BssSI C'ACGA_G
Cuts at: 0 1027 1149
Size: 1027 122
BstYI r'GATC_y
Cuts at: 0 115 359 1116 1149
Size: 115 244 757 33
Fragments arranged by size:
757 244 115 33
Bsu36I CC'TnA_GG
Cuts at: 0 286 601 1149
Size: 286 315 548
Fragments arranged by size:
548 315 286
BtgI C'CryG_G
Cuts at: 0 620 1149
Size: 620 529
BtsI GCAGTG_nn'
Cuts at: 0 760 1149
Size: 760 389
DraIII CAC_nnn'GTG
Cuts at: 0 882 1149
Size: 882 267
EaeI y'GGCC_r
Cuts at: 0 482 803 1149
Size: 482 321 346
Fragments arranged by size:
482 346 321
EarI CTCTTCn'nnn_
Cuts at: 0 421 1149
Size: 421 728
EcoICRI GAG'CTC
Cuts at: 0 386 414 472 1026 1149
Size: 386 28 58 554 123
Fragments arranged by size:
554 386 123 58 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 450 454 892 1149
Size: 322 128 4 438 257
Fragments arranged by size:
438 322 257 128 4
EcoO109I rG'GnC_Cy
Cuts at: 0 282 1041 1149
Size: 282 759 108
Fragments arranged by size:
759 282 108
EcoRV GAT'ATC
Cuts at: 0 580 1149
Size: 580 569
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 285 317 522 554 702 715 734 747
Size: 285 32 205 32 148 13 19 13
Cuts at: 747 1101 1108 1133 1140 1149
Size: 354 7 25 7 9
Fragments arranged by size:
354 285 205 148 32 32 25 19
13 13 9 7 7
MscI TGG'CCA
Cuts at: 0 484 805 1149
Size: 484 321 344
Fragments arranged by size:
484 344 321
MslI CAynn'nnrTG
Cuts at: 0 152 664 1079 1149
Size: 152 512 415 70
Fragments arranged by size:
512 415 152 70
MspA1I CmG'CkG
Cuts at: 0 860 1009 1149
Size: 860 149 140
Fragments arranged by size:
860 149 140
NcoI C'CATG_G
Cuts at: 0 620 1149
Size: 620 529
NsiI A_TGCA'T
Cuts at: 0 551 1149
Size: 551 598
NspI r_CATG'y
Cuts at: 0 553 674 1149
Size: 553 121 475
Fragments arranged by size:
553 475 121
PasI CC'CwG_GG
Cuts at: 0 204 1149
Size: 204 945
PflMI CCAn_nnn'nTGG
Cuts at: 0 432 1149
Size: 432 717
PfoI T'CCnGG_A
Cuts at: 0 54 338 1043 1149
Size: 54 284 705 106
Fragments arranged by size:
705 284 106 54
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 820 852 1149
Size: 820 32 297
Fragments arranged by size:
820 297 32
PpuMI rG'GwC_Cy
Cuts at: 0 282 1041 1149
Size: 282 759 108
Fragments arranged by size:
759 282 108
PsiI TTA'TAA
Cuts at: 0 89 1149
Size: 89 1060
PspOMI G'GGCC_C
Cuts at: 0 9 1149
Size: 9 1140
PstI C_TGCA'G
Cuts at: 0 566 1014 1040 1149
Size: 566 448 26 109
Fragments arranged by size:
566 448 109 26
PvuII CAG'CTG
Cuts at: 0 1009 1149
Size: 1009 140
SacI G_AGCT'C
Cuts at: 0 388 416 474 1028 1149
Size: 388 28 58 554 121
Fragments arranged by size:
554 388 121 58 28
SapI GCTCTTCn'nnn_
Cuts at: 0 421 1149
Size: 421 728
SbfI CC_TGCA'GG
Cuts at: 0 566 1040 1149
Size: 566 474 109
Fragments arranged by size:
566 474 109
SfcI C'TryA_G
Cuts at: 0 562 1010 1036 1149
Size: 562 448 26 113
Fragments arranged by size:
562 448 113 26
SphI G_CATG'C
Cuts at: 0 553 1149
Size: 553 596
StuI AGG'CCT
Cuts at: 0 646 1149
Size: 646 503
StyI C'CwwG_G
Cuts at: 0 479 620 819 1149
Size: 479 141 199 330
Fragments arranged by size:
479 330 199 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 763 789 1070 1149
Size: 763 26 281 79
Fragments arranged by size:
763 281 79 26
Enzymes that do cut:
AarI AcuI AloI AlwNI ApaI ApoI BanI BanII
BbsI BfrBI BglII BlpI Bme1580I BmrI BpmI BsaI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BssSI BstYI Bsu36I BtgI BtsI DraIII EaeI
EarI EcoICRI Eco57MI EcoO109I EcoRV Hin4I MscI MslI
MspA1I NcoI NsiI NspI PasI PflMI PfoI PpiI
PpuMI PsiI PspOMI PstI PvuII SacI SapI SbfI
SfcI SphI StuI StyI TaqII
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BglI BmgBI BmtI BplI Bpu10I BpuEI BsaAI BsaBI
BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I
BtgZI ClaI CspCI DraI DrdI EagI EciI EcoNI
EcoRI FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NotI NruI PacI PciI PmeI
PmlI PshAI PspXI PsrI PvuI RsrII SacII SalI
SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI SspI SwaI TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI