Restriction map for
pFN21AE2541
(Linear) (Six-base) MAPSORT of: KIEE2541.seq Check: 9797 from: 1 to: 969
KIEE2541.orf
With 182 enzymes: SgfI *
August 29, 2014 10:08 ..
AccI GT'mk_AC
Cuts at: 0 203 969
Size: 203 766
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 780 947 969
Size: 780 167 22
Fragments arranged by size:
780 167 22
AgeI A'CCGG_T
Cuts at: 0 198 969
Size: 198 771
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 381 969
Size: 381 588
AlwNI CAG_nnn'CTG
Cuts at: 0 301 369 969
Size: 301 68 600
Fragments arranged by size:
600 301 68
ApaI G_GGCC'C
Cuts at: 0 710 969
Size: 710 259
ApaLI G'TGCA_C
Cuts at: 0 315 969
Size: 315 654
ApoI r'AATT_y
Cuts at: 0 121 769 969
Size: 121 648 200
Fragments arranged by size:
648 200 121
BamHI G'GATC_C
Cuts at: 0 160 969
Size: 160 809
BanI G'GyrC_C
Cuts at: 0 625 969
Size: 625 344
BanII G_rGCy'C
Cuts at: 0 710 969
Size: 710 259
BbsI GAAGACnn'nnnn_
Cuts at: 0 339 744 953 969
Size: 339 405 209 16
Fragments arranged by size:
405 339 209 16
BbvCI CC'TCA_GC
Cuts at: 0 151 969
Size: 151 818
Bme1580I G_kGCm'C
Cuts at: 0 319 710 969
Size: 319 391 259
Fragments arranged by size:
391 319 259
BmrI ACTGGGnnnn_n'
Cuts at: 0 183 357 410 969
Size: 183 174 53 559
Fragments arranged by size:
559 183 174 53
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 742 969
Size: 742 227
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 908 968 969
Size: 908 60 1
Fragments arranged by size:
908 60 1
Bpu10I CC'TnA_GC
Cuts at: 0 151 969
Size: 151 818
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 969
Size: 77 892
BsaI GGTCTCn'nnnn_
Cuts at: 0 481 969
Size: 481 488
BsaBI GATnn'nnATC
Cuts at: 0 331 969
Size: 331 638
BsaWI w'CCGG_w
Cuts at: 0 198 969
Size: 198 771
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 139 937 969
Size: 139 798 32
Fragments arranged by size:
798 139 32
BseYI C'CCAG_C
Cuts at: 0 809 969
Size: 809 160
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 969
Size: 274 695
BsiEI CG_ry'CG
Cuts at: 0 203 969
Size: 203 766
BsiHKAI G_wGCw'C
Cuts at: 0 319 969
Size: 319 650
BsmBI CGTCTCn'nnnn_
Cuts at: 0 856 969
Size: 856 113
Bsp1286I G_dGCh'C
Cuts at: 0 319 710 969
Size: 319 391 259
Fragments arranged by size:
391 319 259
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 666 969
Size: 666 303
BsrDI GCAATG_nn'
Cuts at: 0 239 969
Size: 239 730
BsrFI r'CCGG_y
Cuts at: 0 198 969
Size: 198 771
BstXI CCAn_nnnn'nTGG
Cuts at: 0 423 969
Size: 423 546
BstYI r'GATC_y
Cuts at: 0 160 969
Size: 160 809
BtgI C'CryG_G
Cuts at: 0 248 308 443 969
Size: 248 60 135 526
Fragments arranged by size:
526 248 135 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 318 339 969
Size: 318 21 630
Fragments arranged by size:
630 318 21
BtsI GCAGTG_nn'
Cuts at: 0 262 302 969
Size: 262 40 667
Fragments arranged by size:
667 262 40
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 159 194 969
Size: 159 35 775
Fragments arranged by size:
775 159 35
DraI TTT'AAA
Cuts at: 0 786 969
Size: 786 183
EaeI y'GGCC_r
Cuts at: 0 128 264 279 305 969
Size: 128 136 15 26 664
Fragments arranged by size:
664 136 128 26 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 780 908 947 968 969
Size: 780 128 39 21 1
Fragments arranged by size:
780 128 39 21 1
EcoRI G'AATT_C
Cuts at: 0 769 969
Size: 769 200
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 363 969
Size: 363 606
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 47 79 152 184 377 409 943 969
Size: 47 32 73 32 193 32 534 26
Fragments arranged by size:
534 193 73 47 32 32 32 26
HincII GTy'rAC
Cuts at: 0 204 912 969
Size: 204 708 57
Fragments arranged by size:
708 204 57
HindIII A'AGCT_T
Cuts at: 0 95 969
Size: 95 874
MfeI C'AATT_G
Cuts at: 0 505 575 593 969
Size: 505 70 18 376
Fragments arranged by size:
505 376 70 18
MscI TGG'CCA
Cuts at: 0 130 266 281 307 969
Size: 130 136 15 26 662
Fragments arranged by size:
662 136 130 26 15
MslI CAynn'nnrTG
Cuts at: 0 67 835 969
Size: 67 768 134
Fragments arranged by size:
768 134 67
MspA1I CmG'CkG
Cuts at: 0 118 275 466 530 969
Size: 118 157 191 64 439
Fragments arranged by size:
439 191 157 118 64
NcoI C'CATG_G
Cuts at: 0 248 308 443 969
Size: 248 60 135 526
Fragments arranged by size:
526 248 135 60
NspI r_CATG'y
Cuts at: 0 901 969
Size: 901 68
PflMI CCAn_nnn'nTGG
Cuts at: 0 173 369 969
Size: 173 196 600
Fragments arranged by size:
600 196 173
PshAI GACnn'nnGTC
Cuts at: 0 550 969
Size: 550 419
PspOMI G'GGCC_C
Cuts at: 0 706 969
Size: 706 263
PstI C_TGCA'G
Cuts at: 0 297 663 969
Size: 297 366 306
Fragments arranged by size:
366 306 297
PvuII CAG'CTG
Cuts at: 0 530 969
Size: 530 439
SalI G'TCGA_C
Cuts at: 0 202 969
Size: 202 767
SfcI C'TryA_G
Cuts at: 0 293 659 969
Size: 293 366 310
Fragments arranged by size:
366 310 293
SmlI C'TyrA_G
Cuts at: 0 92 969
Size: 92 877
StuI AGG'CCT
Cuts at: 0 431 969
Size: 431 538
StyI C'CwwG_G
Cuts at: 0 248 308 443 969
Size: 248 60 135 526
Fragments arranged by size:
526 248 135 60
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 274 969
Size: 274 695
Enzymes that do cut:
AccI AcuI AgeI AlfI AlwNI ApaI ApaLI ApoI
BamHI BanI BanII BbsI BbvCI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI
BstXI BstYI BtgI BtgZI BtsI CspCI DraI EaeI
Eco57MI EcoRI FalI Hin4I HincII HindIII MfeI MscI
MslI MspA1I NcoI NspI PflMI PshAI PspOMI PstI
PvuII SalI SfcI SmlI StuI StyI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AhdI
AleI AloI AscI AseI AsiSI AvaI AvrII BaeI
BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmtI BsaAI BsaHI BsaXI BsiWI BsmI BspEI
BspHI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I ClaI DraIII DrdI EagI EarI EciI
EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII
HpaI KasI KpnI MluI MmeI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PsiI PspXI PsrI
PvuI RsrII SacI SacII SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI