Restriction map for
pFN21AE2540
(Linear) (Six-base) MAPSORT of: KIEE2540.seq Check: 189 from: 1 to: 2922
KIEE2540.orf
With 182 enzymes: SgfI *
September 19, 2014 17:03 ..
AccI GT'mk_AC
Cuts at: 0 995 2922
Size: 995 1927
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 301 424 1322 1874 2628 2686 2755
Size: 218 83 123 898 552 754 58 69
Cuts at: 2755 2922
Size: 167
Fragments arranged by size:
898 754 552 218 167 123 83 69
58
AfeI AGC'GCT
Cuts at: 0 148 2922
Size: 148 2774
AflIII A'CryG_T
Cuts at: 0 435 2922
Size: 435 2487
AgeI A'CCGG_T
Cuts at: 0 654 2922
Size: 654 2268
AlwNI CAG_nnn'CTG
Cuts at: 0 2041 2119 2733 2922
Size: 2041 78 614 189
Fragments arranged by size:
2041 614 189 78
ApaI G_GGCC'C
Cuts at: 0 2145 2922
Size: 2145 777
ApoI r'AATT_y
Cuts at: 0 593 644 2485 2922
Size: 593 51 1841 437
Fragments arranged by size:
1841 593 437 51
AvaI C'yCGr_G
Cuts at: 0 584 2922
Size: 584 2338
BamHI G'GATC_C
Cuts at: 0 1797 2922
Size: 1797 1125
BanI G'GyrC_C
Cuts at: 0 1006 1494 2761 2922
Size: 1006 488 1267 161
Fragments arranged by size:
1267 1006 488 161
BanII G_rGCy'C
Cuts at: 0 20 2145 2380 2642 2922
Size: 20 2125 235 262 280
Fragments arranged by size:
2125 280 262 235 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 227 1045 1131 2922
Size: 227 818 86 1791
Fragments arranged by size:
1791 818 227 86
BclI T'GATC_A
Cuts at: 0 362 2922
Size: 362 2560
BglI GCCn_nnn'nGGC
Cuts at: 0 1304 1309 2922
Size: 1304 5 1613
Fragments arranged by size:
1613 1304 5
BlpI GC'TnA_GC
Cuts at: 0 834 2922
Size: 834 2088
Bme1580I G_kGCm'C
Cuts at: 0 86 1009 1497 1607 1619 2145 2922
Size: 86 923 488 110 12 526 777
Fragments arranged by size:
923 777 526 488 110 86 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 73 962 2662 2922
Size: 73 889 1700 260
Fragments arranged by size:
1700 889 260 73
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 985 1619 1712 2069 2292 2466 2922
Size: 985 634 93 357 223 174 456
Fragments arranged by size:
985 634 456 357 223 174 93
Bpu10I CC'TnA_GC
Cuts at: 0 2510 2636 2922
Size: 2510 126 286
Fragments arranged by size:
2510 286 126
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 2922
Size: 193 2729
BsaI GGTCTCn'nnnn_
Cuts at: 0 773 2444 2922
Size: 773 1671 478
Fragments arranged by size:
1671 773 478
BsaBI GATnn'nnATC
Cuts at: 0 2729 2922
Size: 2729 193
BsaWI w'CCGG_w
Cuts at: 0 484 654 2922
Size: 484 170 2268
Fragments arranged by size:
2268 484 170
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 34 64 1760 1790 2922
Size: 34 30 1696 30 1132
Fragments arranged by size:
1696 1132 34 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 157 1231 2922
Size: 157 1074 1691
Fragments arranged by size:
1691 1074 157
BseYI C'CCAG_C
Cuts at: 0 826 1287 1318 1477 2123 2742 2922
Size: 826 461 31 159 646 619 180
Fragments arranged by size:
826 646 619 461 180 159 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 2922
Size: 106 2816
BsiHKAI G_wGCw'C
Cuts at: 0 859 948 2417 2642 2922
Size: 859 89 1469 225 280
Fragments arranged by size:
1469 859 280 225 89
BsmI GAATG_Cn'
Cuts at: 0 1431 1920 2922
Size: 1431 489 1002
Fragments arranged by size:
1431 1002 489
Bsp1286I G_dGCh'C
Cuts at: 0 20 86 859 948 1009 1497 1607 1619
Size: 20 66 773 89 61 488 110 12
Cuts at: 1619 2145 2380 2417 2642 2922
Size: 526 235 37 225 280
Fragments arranged by size:
773 526 488 280 235 225 110 89
66 61 37 20 12
BsrDI GCAATG_nn'
Cuts at: 0 1109 2598 2897 2922
Size: 1109 1489 299 25
Fragments arranged by size:
1489 1109 299 25
BsrFI r'CCGG_y
Cuts at: 0 654 1303 2922
Size: 654 649 1619
Fragments arranged by size:
1619 654 649
BssHII G'CGCG_C
Cuts at: 0 2097 2922
Size: 2097 825
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2119 2922
Size: 2119 803
BstEII G'GTnAC_C
Cuts at: 0 1263 2922
Size: 1263 1659
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1816 2922
Size: 1816 1106
BstYI r'GATC_y
Cuts at: 0 306 950 1098 1797 2730 2922
Size: 306 644 148 699 933 192
Fragments arranged by size:
933 699 644 306 192 148
BtgI C'CryG_G
Cuts at: 0 2155 2922
Size: 2155 767
BtsI GCAGTG_nn'
Cuts at: 0 1263 2922
Size: 1263 1659
ClaI AT'CG_AT
Cuts at: 0 2696 2922
Size: 2696 226
DraI TTT'AAA
Cuts at: 0 723 1709 2922
Size: 723 986 1213
Fragments arranged by size:
1213 986 723
DrdI GACnn_nn'nnGTC
Cuts at: 0 103 1818 2474 2922
Size: 103 1715 656 448
Fragments arranged by size:
1715 656 448 103
EaeI y'GGCC_r
Cuts at: 0 2 268 2913 2922
Size: 2 266 2645 9
Fragments arranged by size:
2645 266 9 2
EarI CTCTTCn'nnn_
Cuts at: 0 2647 2922
Size: 2647 275
EcoICRI GAG'CTC
Cuts at: 0 2640 2922
Size: 2640 282
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 301 424 985 1322 1619 1712 1874
Size: 218 83 123 561 337 297 93 162
Cuts at: 1874 2069 2292 2466 2628 2686 2755 2922
Size: 195 223 174 162 58 69 167
Fragments arranged by size:
561 337 297 223 218 195 174 167
162 162 123 93 83 69 58
EcoO109I rG'GnC_Cy
Cuts at: 0 1375 2141 2922
Size: 1375 766 781
Fragments arranged by size:
1375 781 766
EcoRI G'AATT_C
Cuts at: 0 593 644 2922
Size: 593 51 2278
Fragments arranged by size:
2278 593 51
EcoRV GAT'ATC
Cuts at: 0 1329 2922
Size: 1329 1593
FspI TGC'GCA
Cuts at: 0 1115 2922
Size: 1115 1807
HaeII r_GCGC'y
Cuts at: 0 150 2922
Size: 150 2772
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 34 66 479 511 1773 1805 1816 1848
Size: 34 32 413 32 1262 32 11 32
Cuts at: 1848 1926 1958 2365 2397 2922
Size: 78 32 407 32 525
Fragments arranged by size:
1262 525 413 407 78 34 32 32
32 32 32 32 11
HincII GTy'rAC
Cuts at: 0 2172 2922
Size: 2172 750
HindIII A'AGCT_T
Cuts at: 0 2815 2922
Size: 2815 107
MfeI C'AATT_G
Cuts at: 0 635 2922
Size: 635 2287
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 77 536 2426 2922
Size: 20 57 459 1890 496
Fragments arranged by size:
1890 496 459 57 20
MscI TGG'CCA
Cuts at: 0 270 2922
Size: 270 2652
MslI CAynn'nnrTG
Cuts at: 0 434 2922
Size: 434 2488
MspA1I CmG'CkG
Cuts at: 0 2119 2305 2918 2922
Size: 2119 186 613 4
Fragments arranged by size:
2119 613 186 4
NaeI GCC'GGC
Cuts at: 0 1305 2922
Size: 1305 1617
NdeI CA'TA_TG
Cuts at: 0 2166 2922
Size: 2166 756
NgoMIV G'CCGG_C
Cuts at: 0 1303 2922
Size: 1303 1619
NspI r_CATG'y
Cuts at: 0 439 1316 1720 1890 2178 2922
Size: 439 877 404 170 288 744
Fragments arranged by size:
877 744 439 404 288 170
PasI CC'CwG_GG
Cuts at: 0 9 2922
Size: 9 2913
PciI A'CATG_T
Cuts at: 0 435 2922
Size: 435 2487
PflMI CCAn_nnn'nTGG
Cuts at: 0 640 1468 1490 2922
Size: 640 828 22 1432
Fragments arranged by size:
1432 828 640 22
PfoI T'CCnGG_A
Cuts at: 0 2849 2922
Size: 2849 73
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 930 962 2528 2560 2922
Size: 930 32 1566 32 362
Fragments arranged by size:
1566 930 362 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1375 2922
Size: 1375 1547
PshAI GACnn'nnGTC
Cuts at: 0 2248 2922
Size: 2248 674
PspOMI G'GGCC_C
Cuts at: 0 2141 2922
Size: 2141 781
PstI C_TGCA'G
Cuts at: 0 2106 2922
Size: 2106 816
PvuII CAG'CTG
Cuts at: 0 2119 2305 2922
Size: 2119 186 617
Fragments arranged by size:
2119 617 186
SacI G_AGCT'C
Cuts at: 0 2642 2922
Size: 2642 280
SanDI GG'GwC_CC
Cuts at: 0 1375 2922
Size: 1375 1547
SapI GCTCTTCn'nnn_
Cuts at: 0 2647 2922
Size: 2647 275
SexAI A'CCwGG_T
Cuts at: 0 542 2922
Size: 542 2380
SfcI C'TryA_G
Cuts at: 0 551 2102 2922
Size: 551 1551 820
Fragments arranged by size:
1551 820 551
SmlI C'TyrA_G
Cuts at: 0 172 2922
Size: 172 2750
SpeI A'CTAG_T
Cuts at: 0 781 2922
Size: 781 2141
SphI G_CATG'C
Cuts at: 0 1890 2922
Size: 1890 1032
SspI AAT'ATT
Cuts at: 0 462 708 1587 2922
Size: 462 246 879 1335
Fragments arranged by size:
1335 879 462 246
StuI AGG'CCT
Cuts at: 0 1346 2922
Size: 1346 1576
StyI C'CwwG_G
Cuts at: 0 353 1347 1949 2922
Size: 353 994 602 973
Fragments arranged by size:
994 973 602 353
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1491 1696 2468 2922
Size: 1491 205 772 454
Fragments arranged by size:
1491 772 454 205
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1760 1792 1905 1937 2922
Size: 1760 32 113 32 985
Fragments arranged by size:
1760 985 113 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 103 1364 2922
Size: 103 1261 1558
Fragments arranged by size:
1558 1261 103
XmnI GAAnn'nnTTC
Cuts at: 0 1899 2922
Size: 1899 1023
Enzymes that do cut:
AccI AcuI AfeI AflIII AgeI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbsI BclI BglI BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BsrDI
BsrFI BssHII BstAPI BstEII BstXI BstYI BtgI BtsI
ClaI DraI DrdI EaeI EarI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII MfeI
MmeI MscI MslI MspA1I NaeI NdeI NgoMIV NspI
PasI PciI PflMI PfoI PpiI PpuMI PshAI PspOMI
PstI PvuII SacI SanDI SapI SexAI SfcI SmlI
SpeI SphI SspI StuI StyI TaqII TstI Tth111I
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AhdI AleI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BcgI BciVI BfrBI BglII BmgBI BmtI BplI
BsaAI BsaHI BsiEI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BsrGI BssSI BstBI BstZ17I Bsu36I BtgZI CspCI
DraIII EagI EciI EcoNI FalI FseI FspAI HpaI
KasI KpnI MluI NarI NcoI NheI NotI NruI
NsiI PacI PmeI PmlI PsiI PspXI PsrI PvuI
RsrII SacII SalI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SrfI SwaI TatI XbaI XcmI
XhoI XmaI ZraI