Restriction map for
pFN21AE2528
(Linear) (Six-base) MAPSORT of: KIEE2528.seq Check: 7518 from: 1 to: 1347
KIEE2528.orf
With 182 enzymes: SgfI *
June 11, 2014 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 715 840 1347
Size: 715 125 507
Fragments arranged by size:
715 507 125
AatII G_ACGT'C
Cuts at: 0 1337 1347
Size: 1337 10
AccI GT'mk_AC
Cuts at: 0 1119 1347
Size: 1119 228
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 98 1347
Size: 36 62 1249
Fragments arranged by size:
1249 62 36
AflIII A'CryG_T
Cuts at: 0 535 1292 1347
Size: 535 757 55
Fragments arranged by size:
757 535 55
AleI CACnn'nnGTG
Cuts at: 0 859 1347
Size: 859 488
ApaI G_GGCC'C
Cuts at: 0 402 1111 1347
Size: 402 709 236
Fragments arranged by size:
709 402 236
ApoI r'AATT_y
Cuts at: 0 513 700 1347
Size: 513 187 647
Fragments arranged by size:
647 513 187
AvaI C'yCGr_G
Cuts at: 0 893 1347
Size: 893 454
AvrII C'CTAG_G
Cuts at: 0 846 1347
Size: 846 501
BanI G'GyrC_C
Cuts at: 0 1150 1347
Size: 1150 197
BanII G_rGCy'C
Cuts at: 0 402 886 1111 1347
Size: 402 484 225 236
Fragments arranged by size:
484 402 236 225
BbeI G_GCGC'C
Cuts at: 0 1154 1347
Size: 1154 193
Bme1580I G_kGCm'C
Cuts at: 0 402 1111 1347
Size: 402 709 236
Fragments arranged by size:
709 402 236
BmrI ACTGGGnnnn_n'
Cuts at: 0 184 633 1347
Size: 184 449 714
Fragments arranged by size:
714 449 184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 751 1091 1347
Size: 751 340 256
Fragments arranged by size:
751 340 256
Bpu10I CC'TnA_GC
Cuts at: 0 436 1347
Size: 436 911
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 687 1347
Size: 687 660
BsaI GGTCTCn'nnnn_
Cuts at: 0 31 1347
Size: 31 1316
BsaHI Gr'CG_yC
Cuts at: 0 1151 1334 1347
Size: 1151 183 13
Fragments arranged by size:
1151 183 13
BsaWI w'CCGG_w
Cuts at: 0 148 1347
Size: 148 1199
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1219 1347
Size: 1219 128
BseYI C'CCAG_C
Cuts at: 0 772 1110 1347
Size: 772 338 237
Fragments arranged by size:
772 338 237
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 660 812 1169 1347
Size: 631 29 152 357 178
Fragments arranged by size:
631 357 178 152 29
BsiHKAI G_wGCw'C
Cuts at: 0 866 886 1347
Size: 866 20 461
Fragments arranged by size:
866 461 20
Bsp1286I G_dGCh'C
Cuts at: 0 402 866 886 1111 1347
Size: 402 464 20 225 236
Fragments arranged by size:
464 402 236 225 20
BspEI T'CCGG_A
Cuts at: 0 148 1347
Size: 148 1199
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 603 715 840 1347
Size: 603 112 125 507
Fragments arranged by size:
603 507 125 112
BsrBI CCG'CTC
Cuts at: 0 735 1347
Size: 735 612
BsrDI GCAATG_nn'
Cuts at: 0 233 289 1347
Size: 233 56 1058
Fragments arranged by size:
1058 233 56
BsrGI T'GTAC_A
Cuts at: 0 2 1347
Size: 2 1345
BssSI C'ACGA_G
Cuts at: 0 836 1347
Size: 836 511
BstYI r'GATC_y
Cuts at: 0 440 800 1347
Size: 440 360 547
Fragments arranged by size:
547 440 360
BtgI C'CryG_G
Cuts at: 0 248 500 521 1271 1347
Size: 248 252 21 750 76
Fragments arranged by size:
750 252 248 76 21
DraI TTT'AAA
Cuts at: 0 180 1347
Size: 180 1167
DrdI GACnn_nn'nnGTC
Cuts at: 0 259 1347
Size: 259 1088
EarI CTCTTCn'nnn_
Cuts at: 0 23 650 1225 1347
Size: 23 627 575 122
Fragments arranged by size:
627 575 122 23
EcoICRI GAG'CTC
Cuts at: 0 884 1347
Size: 884 463
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 98 751 1091 1347
Size: 36 62 653 340 256
Fragments arranged by size:
653 340 256 62 36
EcoO109I rG'GnC_Cy
Cuts at: 0 184 398 1347
Size: 184 214 949
Fragments arranged by size:
949 214 184
HaeII r_GCGC'y
Cuts at: 0 1154 1347
Size: 1154 193
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1123 1155 1237 1269 1319 1347
Size: 1123 32 82 32 50 28
Fragments arranged by size:
1123 82 50 32 32 28
HincII GTy'rAC
Cuts at: 0 264 996 1347
Size: 264 732 351
Fragments arranged by size:
732 351 264
HindIII A'AGCT_T
Cuts at: 0 555 1347
Size: 555 792
HpaI GTT'AAC
Cuts at: 0 996 1347
Size: 996 351
KasI G'GCGC_C
Cuts at: 0 1150 1347
Size: 1150 197
MslI CAynn'nnrTG
Cuts at: 0 407 859 940 1347
Size: 407 452 81 407
Fragments arranged by size:
452 407 407 81
NarI GG'CG_CC
Cuts at: 0 1151 1347
Size: 1151 196
NcoI C'CATG_G
Cuts at: 0 248 500 521 1271 1347
Size: 248 252 21 750 76
Fragments arranged by size:
750 252 248 76 21
NspI r_CATG'y
Cuts at: 0 539 1296 1347
Size: 539 757 51
Fragments arranged by size:
757 539 51
PciI A'CATG_T
Cuts at: 0 535 1292 1347
Size: 535 757 55
Fragments arranged by size:
757 535 55
PfoI T'CCnGG_A
Cuts at: 0 122 445 1347
Size: 122 323 902
Fragments arranged by size:
902 323 122
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 706 738 1347
Size: 706 32 609
Fragments arranged by size:
706 609 32
PpuMI rG'GwC_Cy
Cuts at: 0 184 1347
Size: 184 1163
PspOMI G'GGCC_C
Cuts at: 0 398 1107 1347
Size: 398 709 240
Fragments arranged by size:
709 398 240
SacI G_AGCT'C
Cuts at: 0 886 1347
Size: 886 461
SfcI C'TryA_G
Cuts at: 0 255 465 1120 1347
Size: 255 210 655 227
Fragments arranged by size:
655 255 227 210
SfoI GGC'GCC
Cuts at: 0 1152 1347
Size: 1152 195
SmlI C'TyrA_G
Cuts at: 0 666 893 1347
Size: 666 227 454
Fragments arranged by size:
666 454 227
StyI C'CwwG_G
Cuts at: 0 248 500 521 846 908 1127 1271 1347
Size: 248 252 21 325 62 219 144 76
Fragments arranged by size:
325 252 248 219 144 76 62 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 111 892 1347
Size: 111 781 455
Fragments arranged by size:
781 455 111
TatI w'GTAC_w
Cuts at: 0 2 1347
Size: 2 1345
XhoI C'TCGA_G
Cuts at: 0 893 1347
Size: 893 454
XmnI GAAnn'nnTTC
Cuts at: 0 514 1347
Size: 514 833
ZraI GAC'GTC
Cuts at: 0 1335 1347
Size: 1335 12
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AleI ApaI ApoI
AvaI AvrII BanI BanII BbeI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI BsrGI BssSI
BstYI BtgI DraI DrdI EarI EcoICRI Eco57MI EcoO109I
HaeII Hin4I HincII HindIII HpaI KasI MslI NarI
NcoI NspI PciI PfoI PpiI PpuMI PspOMI SacI
SfcI SfoI SmlI StyI TaqII TatI XhoI XmnI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI
AlwNI ApaLI AscI AseI AsiSI BaeI BamHI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmtI BplI BsaAI BsaBI BsaXI BsiEI BsiWI
BsmI BsmBI BspHI BsrFI BssHII BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII
EaeI EagI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI KpnI MfeI MluI MmeI MscI MspA1I
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PmeI PmlI PshAI PsiI PspXI PsrI
PstI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XcmI XmaI