Restriction map for
pFN21AE2526
(Linear) (Six-base) MAPSORT of: KIEE2526.seq Check: 5469 from: 1 to: 576
KIEE2526.orf
With 182 enzymes: SgfI *
June 11, 2014 14:42 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 462 576
Size: 462 114
AvaI C'yCGr_G
Cuts at: 0 348 527 576
Size: 348 179 49
Fragments arranged by size:
348 179 49
BanI G'GyrC_C
Cuts at: 0 271 322 576
Size: 271 51 254
Fragments arranged by size:
271 254 51
BanII G_rGCy'C
Cuts at: 0 337 548 576
Size: 337 211 28
Fragments arranged by size:
337 211 28
BbeI G_GCGC'C
Cuts at: 0 275 576
Size: 275 301
BbsI GAAGACnn'nnnn_
Cuts at: 0 99 468 576
Size: 99 369 108
Fragments arranged by size:
369 108 99
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 576
Size: 562 14
BsaAI yAC'GTr
Cuts at: 0 216 576
Size: 216 360
BsaHI Gr'CG_yC
Cuts at: 0 272 434 576
Size: 272 162 142
Fragments arranged by size:
272 162 142
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 223 253 576
Size: 223 30 323
Fragments arranged by size:
323 223 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 244 576
Size: 244 332
BseYI C'CCAG_C
Cuts at: 0 87 148 576
Size: 87 61 428
Fragments arranged by size:
428 87 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 271 576
Size: 187 84 305
Fragments arranged by size:
305 187 84
BsiEI CG_ry'CG
Cuts at: 0 429 576
Size: 429 147
BsiHKAI G_wGCw'C
Cuts at: 0 548 576
Size: 548 28
BsmI GAATG_Cn'
Cuts at: 0 258 576
Size: 258 318
BsmBI CGTCTCn'nnnn_
Cuts at: 0 349 576
Size: 349 227
Bsp1286I G_dGCh'C
Cuts at: 0 337 548 576
Size: 337 211 28
Fragments arranged by size:
337 211 28
BsrFI r'CCGG_y
Cuts at: 0 17 576
Size: 17 559
BssSI C'ACGA_G
Cuts at: 0 160 576
Size: 160 416
BstXI CCAn_nnnn'nTGG
Cuts at: 0 410 576
Size: 410 166
BtgI C'CryG_G
Cuts at: 0 11 339 576
Size: 11 328 237
Fragments arranged by size:
328 237 11
BtsI GCAGTG_nn'
Cuts at: 0 10 244 576
Size: 10 234 332
Fragments arranged by size:
332 234 10
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 371 406 576
Size: 371 35 170
Fragments arranged by size:
371 170 35
EaeI y'GGCC_r
Cuts at: 0 342 426 464 564 576
Size: 342 84 38 100 12
Fragments arranged by size:
342 100 84 38 12
EagI C'GGCC_G
Cuts at: 0 426 576
Size: 426 150
EarI CTCTTCn'nnn_
Cuts at: 0 113 322 508 518 576
Size: 113 209 186 10 58
Fragments arranged by size:
209 186 113 58 10
EcoICRI GAG'CTC
Cuts at: 0 546 576
Size: 546 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 462 576
Size: 462 114
EcoO109I rG'GnC_Cy
Cuts at: 0 531 570 576
Size: 531 39 6
Fragments arranged by size:
531 39 6
HaeII r_GCGC'y
Cuts at: 0 275 576
Size: 275 301
KasI G'GCGC_C
Cuts at: 0 271 576
Size: 271 305
MscI TGG'CCA
Cuts at: 0 566 576
Size: 566 10
MslI CAynn'nnrTG
Cuts at: 0 412 576
Size: 412 164
MspA1I CmG'CkG
Cuts at: 0 58 341 388 576
Size: 58 283 47 188
Fragments arranged by size:
283 188 58 47
NaeI GCC'GGC
Cuts at: 0 19 576
Size: 19 557
NarI GG'CG_CC
Cuts at: 0 272 576
Size: 272 304
NgoMIV G'CCGG_C
Cuts at: 0 17 576
Size: 17 559
PflMI CCAn_nnn'nTGG
Cuts at: 0 165 576
Size: 165 411
PmlI CAC'GTG
Cuts at: 0 216 576
Size: 216 360
PpuMI rG'GwC_Cy
Cuts at: 0 570 576
Size: 570 6
PshAI GACnn'nnGTC
Cuts at: 0 70 576
Size: 70 506
PstI C_TGCA'G
Cuts at: 0 239 576
Size: 239 337
PvuII CAG'CTG
Cuts at: 0 388 576
Size: 388 188
SacI G_AGCT'C
Cuts at: 0 548 576
Size: 548 28
SacII CC_GC'GG
Cuts at: 0 342 576
Size: 342 234
SapI GCTCTTCn'nnn_
Cuts at: 0 322 576
Size: 322 254
SfcI C'TryA_G
Cuts at: 0 235 576
Size: 235 341
SfoI GGC'GCC
Cuts at: 0 273 576
Size: 273 303
SmlI C'TyrA_G
Cuts at: 0 541 576
Size: 541 35
StyI C'CwwG_G
Cuts at: 0 329 576
Size: 329 247
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 221 231 499 576
Size: 221 10 268 77
Fragments arranged by size:
268 221 77 10
TatI w'GTAC_w
Cuts at: 0 363 576
Size: 363 213
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 223 255 576
Size: 223 32 321
Fragments arranged by size:
321 223 32
Tth111I GACn'n_nGTC
Cuts at: 0 472 576
Size: 472 104
Enzymes that do cut:
AcuI AvaI BanI BanII BbeI BbsI BpuEI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BsrFI BssSI BstXI BtgI BtsI CspCI
EaeI EagI EarI EcoICRI Eco57MI EcoO109I HaeII KasI
MscI MslI MspA1I NaeI NarI NgoMIV PflMI PmlI
PpuMI PshAI PstI PvuII SacI SacII SapI SfcI
SfoI SmlI StyI TaqII TatI TstI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BsaI BsaBI
BsaWI BsiWI BspEI BspHI BspMI BsrBI BsrDI BsrGI
BssHII BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI
ClaI DraI DraIII DrdI EciI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PfoI PmeI
PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SalI
SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI
XcmI XhoI XmaI XmnI ZraI