Restriction map for
pFN21AE2520
(Linear) (Six-base) MAPSORT of: KIEE2520.seq Check: 5417 from: 1 to: 1773
KIEE2520.orf
With 182 enzymes: SgfI *
March 20, 2014 14:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 962 1693 1773
Size: 962 731 80
Fragments arranged by size:
962 731 80
AhdI GACnn_n'nnGTC
Cuts at: 0 1578 1773
Size: 1578 195
AlwNI CAG_nnn'CTG
Cuts at: 0 967 1435 1752 1773
Size: 967 468 317 21
Fragments arranged by size:
967 468 317 21
ApaI G_GGCC'C
Cuts at: 0 1007 1773
Size: 1007 766
ApoI r'AATT_y
Cuts at: 0 333 422 1773
Size: 333 89 1351
Fragments arranged by size:
1351 333 89
BanI G'GyrC_C
Cuts at: 0 69 1773
Size: 69 1704
BanII G_rGCy'C
Cuts at: 0 1007 1730 1773
Size: 1007 723 43
Fragments arranged by size:
1007 723 43
BbsI GAAGACnn'nnnn_
Cuts at: 0 288 438 1157 1773
Size: 288 150 719 616
Fragments arranged by size:
719 616 288 150
BciVI GTATCCnnnnn_n'
Cuts at: 0 1218 1773
Size: 1218 555
BfrBI ATG'CAT
Cuts at: 0 315 1773
Size: 315 1458
BglI GCCn_nnn'nGGC
Cuts at: 0 1354 1773
Size: 1354 419
Bme1580I G_kGCm'C
Cuts at: 0 1007 1350 1773
Size: 1007 343 423
Fragments arranged by size:
1007 423 343
BmrI ACTGGGnnnn_n'
Cuts at: 0 1622 1773
Size: 1622 151
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1245 1773
Size: 1245 528
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 502 562 592 631 649 667 724
Size: 487 15 60 30 39 18 18 57
Cuts at: 724 1518 1773
Size: 794 255
Fragments arranged by size:
794 487 255 60 57 39 30 18
18 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 1773
Size: 371 1402
BsaI GGTCTCn'nnnn_
Cuts at: 0 268 1773
Size: 268 1505
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1023 1053 1773
Size: 1023 30 720
Fragments arranged by size:
1023 720 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 815 935 983 1007 1043 1052 1076 1079
Size: 815 120 48 24 36 9 24 3
Cuts at: 1079 1377 1773
Size: 298 396
Fragments arranged by size:
815 396 298 120 48 36 24 24
9 3
BseYI C'CCAG_C
Cuts at: 0 840 1773
Size: 840 933
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 953 1773
Size: 953 820
BsiEI CG_ry'CG
Cuts at: 0 893 1773
Size: 893 880
BsiHKAI G_wGCw'C
Cuts at: 0 1730 1773
Size: 1730 43
BsmI GAATG_Cn'
Cuts at: 0 1254 1773
Size: 1254 519
Bsp1286I G_dGCh'C
Cuts at: 0 1007 1350 1730 1773
Size: 1007 343 380 43
Fragments arranged by size:
1007 380 343 43
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 390 1773
Size: 390 1383
BsrBI CCG'CTC
Cuts at: 0 471 615 633 651 1773
Size: 471 144 18 18 1122
Fragments arranged by size:
1122 471 144 18 18
BsrDI GCAATG_nn'
Cuts at: 0 355 1773
Size: 355 1418
BsrFI r'CCGG_y
Cuts at: 0 888 1773
Size: 888 885
BstAPI GCAn_nnn'nTGC
Cuts at: 0 31 1773
Size: 31 1742
BtgI C'CryG_G
Cuts at: 0 1293 1773
Size: 1293 480
DrdI GACnn_nn'nnGTC
Cuts at: 0 169 1773
Size: 169 1604
EaeI y'GGCC_r
Cuts at: 0 890 1355 1773
Size: 890 465 418
Fragments arranged by size:
890 465 418
EagI C'GGCC_G
Cuts at: 0 890 1773
Size: 890 883
EarI CTCTTCn'nnn_
Cuts at: 0 855 1458 1718 1773
Size: 855 603 260 55
Fragments arranged by size:
855 603 260 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1257 1773
Size: 1257 516
EcoICRI GAG'CTC
Cuts at: 0 1728 1773
Size: 1728 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 502 562 592 631 649 667 724
Size: 487 15 60 30 39 18 18 57
Cuts at: 724 962 1518 1693 1773
Size: 238 556 175 80
Fragments arranged by size:
556 487 238 175 80 60 57 39
30 18 18 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 998 1773
Size: 998 775
EcoO109I rG'GnC_Cy
Cuts at: 0 964 1003 1004 1580 1773
Size: 964 39 1 576 193
Fragments arranged by size:
964 576 193 39 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1502 1773
Size: 1502 271
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3 35 92 124 886 918 1564 1596
Size: 3 32 57 32 762 32 646 32
Cuts at: 1596 1773
Size: 177
Fragments arranged by size:
762 646 177 57 32 32 32 32
3
MfeI C'AATT_G
Cuts at: 0 1184 1773
Size: 1184 589
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 491 1773
Size: 491 1282
MscI TGG'CCA
Cuts at: 0 1357 1773
Size: 1357 416
MspA1I CmG'CkG
Cuts at: 0 76 570 765 1180 1773
Size: 76 494 195 415 593
Fragments arranged by size:
593 494 415 195 76
NaeI GCC'GGC
Cuts at: 0 890 1773
Size: 890 883
NgoMIV G'CCGG_C
Cuts at: 0 888 1773
Size: 888 885
NsiI A_TGCA'T
Cuts at: 0 317 1773
Size: 317 1456
NspI r_CATG'y
Cuts at: 0 319 1773
Size: 319 1454
PasI CC'CwG_GG
Cuts at: 0 1000 1773
Size: 1000 773
PflMI CCAn_nnn'nTGG
Cuts at: 0 79 1773
Size: 79 1694
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1255 1287 1773
Size: 1255 32 486
Fragments arranged by size:
1255 486 32
PpuMI rG'GwC_Cy
Cuts at: 0 1580 1773
Size: 1580 193
PspOMI G'GGCC_C
Cuts at: 0 1003 1773
Size: 1003 770
PvuII CAG'CTG
Cuts at: 0 76 570 765 1180 1773
Size: 76 494 195 415 593
Fragments arranged by size:
593 494 415 195 76
SacI G_AGCT'C
Cuts at: 0 1730 1773
Size: 1730 43
SapI GCTCTTCn'nnn_
Cuts at: 0 1718 1773
Size: 1718 55
SmlI C'TyrA_G
Cuts at: 0 386 1773
Size: 386 1387
SphI G_CATG'C
Cuts at: 0 319 1773
Size: 319 1454
StuI AGG'CCT
Cuts at: 0 1450 1773
Size: 1450 323
StyI C'CwwG_G
Cuts at: 0 1486 1549 1773
Size: 1486 63 224
Fragments arranged by size:
1486 224 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 57 1773
Size: 57 1716
TatI w'GTAC_w
Cuts at: 0 206 1462 1773
Size: 206 1256 311
Fragments arranged by size:
1256 311 206
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 212 244 1023 1055 1773
Size: 212 32 779 32 718
Fragments arranged by size:
779 718 212 32 32
Enzymes that do cut:
AcuI AhdI AlwNI ApaI ApoI BanI BanII BbsI
BciVI BfrBI BglI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BtgI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI Hin4I MfeI MmeI MscI MspA1I NaeI NgoMIV
NsiI NspI PasI PflMI PpiI PpuMI PspOMI PvuII
SacI SapI SmlI SphI StuI StyI TaqII TatI
TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BclI
BglII BlpI BmgBI BmtI Bpu10I BsaAI BsaBI BsaHI
BsaWI BsiWI BsmBI BspEI BspHI BsrGI BssHII BssSI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII EcoRI EcoRV FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MluI
MslI NarI NcoI NdeI NheI NotI NruI PacI
PciI PfoI PmeI PmlI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI SwaI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI