Restriction map for
pFN21AE2519
(Linear) (Six-base) MAPSORT of: KIEE2519.seq Check: 8714 from: 1 to: 1665
KIEE2519.orf
With 182 enzymes: SgfI *
April 4, 2014 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 649 1665
Size: 649 1016
AccI GT'mk_AC
Cuts at: 0 1532 1665
Size: 1532 133
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1155 1342 1378 1489 1532 1665
Size: 1155 187 36 111 43 133
Fragments arranged by size:
1155 187 133 111 43 36
AfeI AGC'GCT
Cuts at: 0 430 1267 1665
Size: 430 837 398
Fragments arranged by size:
837 430 398
AflII C'TTAA_G
Cuts at: 0 1654 1665
Size: 1654 11
AflIII A'CryG_T
Cuts at: 0 206 1665
Size: 206 1459
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 800 1665
Size: 800 865
AlwNI CAG_nnn'CTG
Cuts at: 0 1207 1665
Size: 1207 458
ApaLI G'TGCA_C
Cuts at: 0 1246 1665
Size: 1246 419
ApoI r'AATT_y
Cuts at: 0 190 1640 1665
Size: 190 1450 25
Fragments arranged by size:
1450 190 25
BanI G'GyrC_C
Cuts at: 0 414 1665
Size: 414 1251
BanII G_rGCy'C
Cuts at: 0 568 1665
Size: 568 1097
BbsI GAAGACnn'nnnn_
Cuts at: 0 708 1462 1665
Size: 708 754 203
Fragments arranged by size:
754 708 203
BbvCI CC'TCA_GC
Cuts at: 0 1477 1665
Size: 1477 188
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1268 1302 1584 1618 1665
Size: 1268 34 282 34 47
Fragments arranged by size:
1268 282 47 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1288 1665
Size: 1288 377
BglII A'GATC_T
Cuts at: 0 1049 1665
Size: 1049 616
Bme1580I G_kGCm'C
Cuts at: 0 1012 1126 1250 1665
Size: 1012 114 124 415
Fragments arranged by size:
1012 415 124 114
BmgBI CAC'GTC
Cuts at: 0 807 1602 1665
Size: 807 795 63
Fragments arranged by size:
807 795 63
BmrI ACTGGGnnnn_n'
Cuts at: 0 107 730 1665
Size: 107 623 935
Fragments arranged by size:
935 623 107
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 348 1665
Size: 348 1317
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 343 1116 1234 1239 1292 1665
Size: 75 268 773 118 5 53 373
Fragments arranged by size:
773 373 268 118 75 53 5
Bpu10I CC'TnA_GC
Cuts at: 0 1477 1665
Size: 1477 188
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 223 1665
Size: 57 166 1442
Fragments arranged by size:
1442 166 57
BsaBI GATnn'nnATC
Cuts at: 0 1048 1665
Size: 1048 617
BsaHI Gr'CG_yC
Cuts at: 0 1145 1219 1665
Size: 1145 74 446
Fragments arranged by size:
1145 446 74
BsaWI w'CCGG_w
Cuts at: 0 1106 1665
Size: 1106 559
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 865 1363 1444 1665
Size: 865 498 81 221
Fragments arranged by size:
865 498 221 81
BseYI C'CCAG_C
Cuts at: 0 313 532 654 1297 1665
Size: 313 219 122 643 368
Fragments arranged by size:
643 368 313 219 122
BsiHKAI G_wGCw'C
Cuts at: 0 845 934 1250 1665
Size: 845 89 316 415
Fragments arranged by size:
845 415 316 89
BsmI GAATG_Cn'
Cuts at: 0 404 768 1488 1665
Size: 404 364 720 177
Fragments arranged by size:
720 404 364 177
BsmBI CGTCTCn'nnnn_
Cuts at: 0 142 1177 1210 1665
Size: 142 1035 33 455
Fragments arranged by size:
1035 455 142 33
Bsp1286I G_dGCh'C
Cuts at: 0 568 845 934 1012 1126 1250 1665
Size: 568 277 89 78 114 124 415
Fragments arranged by size:
568 415 277 124 114 89 78
BspEI T'CCGG_A
Cuts at: 0 1106 1665
Size: 1106 559
BspHI T'CATG_A
Cuts at: 0 185 1665
Size: 185 1480
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 649 986 1339 1665
Size: 649 337 353 326
Fragments arranged by size:
649 353 337 326
BsrBI CCG'CTC
Cuts at: 0 1095 1665
Size: 1095 570
BsrDI GCAATG_nn'
Cuts at: 0 1185 1665
Size: 1185 480
BsrFI r'CCGG_y
Cuts at: 0 328 1665
Size: 328 1337
BstXI CCAn_nnnn'nTGG
Cuts at: 0 321 1665
Size: 321 1344
BstYI r'GATC_y
Cuts at: 0 1049 1665
Size: 1049 616
BtgI C'CryG_G
Cuts at: 0 132 605 1665
Size: 132 473 1060
Fragments arranged by size:
1060 473 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1100 1665
Size: 1100 565
BtsI GCAGTG_nn'
Cuts at: 0 782 1665
Size: 782 883
EaeI y'GGCC_r
Cuts at: 0 1198 1665
Size: 1198 467
EarI CTCTTCn'nnn_
Cuts at: 0 484 890 1508 1665
Size: 484 406 618 157
Fragments arranged by size:
618 484 406 157
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 343 1116 1155 1234 1239 1292 1342
Size: 75 268 773 39 79 5 53 50
Cuts at: 1342 1378 1489 1532 1665
Size: 36 111 43 133
Fragments arranged by size:
773 268 133 111 79 75 53 50
43 39 36 5
EcoO109I rG'GnC_Cy
Cuts at: 0 64 269 310 732 1294 1665
Size: 64 205 41 422 562 371
Fragments arranged by size:
562 422 371 205 64 41
EcoRV GAT'ATC
Cuts at: 0 489 1665
Size: 489 1176
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1408 1665
Size: 1408 257
HaeII r_GCGC'y
Cuts at: 0 432 1020 1269 1665
Size: 432 588 249 396
Fragments arranged by size:
588 432 396 249
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 193 225 364 396 928 960 1665
Size: 193 32 139 32 532 32 705
Fragments arranged by size:
705 532 193 139 32 32 32
HincII GTy'rAC
Cuts at: 0 1563 1665
Size: 1563 102
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 710 1331 1665
Size: 710 621 334
Fragments arranged by size:
710 621 334
MslI CAynn'nnrTG
Cuts at: 0 364 805 1665
Size: 364 441 860
Fragments arranged by size:
860 441 364
MspA1I CmG'CkG
Cuts at: 0 747 795 1665
Size: 747 48 870
Fragments arranged by size:
870 747 48
NaeI GCC'GGC
Cuts at: 0 330 1665
Size: 330 1335
NcoI C'CATG_G
Cuts at: 0 132 605 1665
Size: 132 473 1060
Fragments arranged by size:
1060 473 132
NgoMIV G'CCGG_C
Cuts at: 0 328 1665
Size: 328 1337
PasI CC'CwG_GG
Cuts at: 0 561 1665
Size: 561 1104
PflMI CCAn_nnn'nTGG
Cuts at: 0 138 661 1665
Size: 138 523 1004
Fragments arranged by size:
1004 523 138
PpuMI rG'GwC_Cy
Cuts at: 0 64 732 1294 1665
Size: 64 668 562 371
Fragments arranged by size:
668 562 371 64
PstI C_TGCA'G
Cuts at: 0 309 983 1265 1665
Size: 309 674 282 400
Fragments arranged by size:
674 400 309 282
PvuII CAG'CTG
Cuts at: 0 747 1665
Size: 747 918
SanDI GG'GwC_CC
Cuts at: 0 64 1665
Size: 64 1601
SapI GCTCTTCn'nnn_
Cuts at: 0 484 1665
Size: 484 1181
SbfI CC_TGCA'GG
Cuts at: 0 309 1665
Size: 309 1356
SfcI C'TryA_G
Cuts at: 0 305 979 1261 1665
Size: 305 674 282 404
Fragments arranged by size:
674 404 305 282
SmlI C'TyrA_G
Cuts at: 0 36 238 1654 1665
Size: 36 202 1416 11
Fragments arranged by size:
1416 202 36 11
StuI AGG'CCT
Cuts at: 0 671 1665
Size: 671 994
StyI C'CwwG_G
Cuts at: 0 68 114 132 605 902 1379 1665
Size: 68 46 18 473 297 477 286
Fragments arranged by size:
477 473 297 286 68 46 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 618 1665
Size: 618 1047
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 754 786 1665
Size: 754 32 879
Fragments arranged by size:
879 754 32
XmnI GAAnn'nnTTC
Cuts at: 0 1102 1665
Size: 1102 563
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AflIII AlfI AlwNI
ApaLI ApoI BanI BanII BbsI BbvCI BcgI BglI
BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaBI BsaHI BsaWI BseRI BseYI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BstXI
BstYI BtgI BtgZI BtsI EaeI EarI Eco57MI EcoO109I
EcoRV FalI HaeII Hin4I HincII MmeI MslI MspA1I
NaeI NcoI NgoMIV PasI PflMI PpuMI PstI PvuII
SanDI SapI SbfI SfcI SmlI StuI StyI TaqII
TstI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AgeI AhdI AleI AloI ApaI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI
BciVI BclI BfrBI BlpI BmtI BsaI BsaAI BsaXI
BsgI BsiEI BsiWI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoRI FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI MscI NarI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TatI Tth111I XbaI XcmI XhoI
XmaI ZraI