(Linear) (Six-base) MAPSORT of: KIEE2517.seq  Check: 2726  from: 1  to: 1323



KIEE2517.orf

 With 182 enzymes: SgfI * 

                        February 25, 2014 15:45  ..

AccI GT'mk_AC

Cuts at:      0      19    1323 
   Size:         19    1304
 
AleI CACnn'nnGTG

Cuts at:      0     673    1323 
   Size:        673     650
 
AlwNI CAG_nnn'CTG

Cuts at:      0      66    1323 
   Size:         66    1257
 
ApoI r'AATT_y

Cuts at:      0     589    1323 
   Size:        589     734
 
BlpI GC'TnA_GC

Cuts at:      0      67    1323 
   Size:         67    1256
 
Bme1580I G_kGCm'C

Cuts at:      0     704    1323 
   Size:        704     619
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1028    1323 
   Size:       1028     295
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     497    1323 
   Size:        497     826
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     122    1323 
   Size:        122    1201
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247    1323 
   Size:        247    1076
 
BsaAI yAC'GTr

Cuts at:      0     414    1323 
   Size:        414     909
 
BsaBI GATnn'nnATC

Cuts at:      0     608     731     839    1323 
   Size:        608     123     108     484

  Fragments arranged by size: 

                 608     484     123     108
 
BsaWI w'CCGG_w

Cuts at:      0     395    1323 
   Size:        395     928
 
BseYI C'CCAG_C

Cuts at:      0    1307    1323 
   Size:       1307      16
 
BsmI GAATG_Cn'

Cuts at:      0     819    1268    1323 
   Size:        819     449      55

  Fragments arranged by size: 

                 819     449      55
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      98    1323 
   Size:         98    1225
 
Bsp1286I G_dGCh'C

Cuts at:      0     704    1323 
   Size:        704     619
 
BsrFI r'CCGG_y

Cuts at:      0      27    1323 
   Size:         27    1296
 
BsrGI T'GTAC_A

Cuts at:      0    1201    1323 
   Size:       1201     122
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1125    1323 
   Size:       1125     198
 
BstYI r'GATC_y

Cuts at:      0     324     499     603    1182    1323 
   Size:        324     175     104     579     141

  Fragments arranged by size: 

                 579     324     175     141     104
 
BtgI C'CryG_G

Cuts at:      0     564    1323 
   Size:        564     759
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     951    1323 
   Size:        951     372
 
BtsI GCAGTG_nn'

Cuts at:      0      78     547    1323 
   Size:         78     469     776

  Fragments arranged by size: 

                 776     469      78
 
EaeI y'GGCC_r

Cuts at:      0      50     561    1323 
   Size:         50     511     762

  Fragments arranged by size: 

                 762     511      50
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     122    1323 
   Size:        122    1201
 
HaeII r_GCGC'y

Cuts at:      0    1308    1323 
   Size:       1308      15
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     510     542     654     686    1323 
   Size:        510      32     112      32     637

  Fragments arranged by size: 

                 637     510     112      32      32
 
HincII GTy'rAC

Cuts at:      0      20    1323 
   Size:         20    1303
 
MscI TGG'CCA

Cuts at:      0     563    1323 
   Size:        563     760
 
MslI CAynn'nnrTG

Cuts at:      0     569     673     707    1223    1323 
   Size:        569     104      34     516     100

  Fragments arranged by size: 

                 569     516     104     100      34
 
NaeI GCC'GGC

Cuts at:      0      29    1323 
   Size:         29    1294
 
NcoI C'CATG_G

Cuts at:      0     564    1323 
   Size:        564     759
 
NgoMIV G'CCGG_C

Cuts at:      0      27    1323 
   Size:         27    1296
 
NspI r_CATG'y

Cuts at:      0      45    1323 
   Size:         45    1278
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     289    1301    1323 
   Size:        289    1012      22

  Fragments arranged by size: 

                1012     289      22
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     698     730    1323 
   Size:        698      32     593

  Fragments arranged by size: 

                 698     593      32
 
PsiI TTA'TAA

Cuts at:      0     839    1323 
   Size:        839     484
 
SalI G'TCGA_C

Cuts at:      0      18    1323 
   Size:         18    1305
 
SfcI C'TryA_G

Cuts at:      0     344     491    1323 
   Size:        344     147     832

  Fragments arranged by size: 

                 832     344     147
 
SgrAI Cr'CCGG_yG

Cuts at:      0      27    1323 
   Size:         27    1296
 
SmlI C'TyrA_G

Cuts at:      0     262    1323 
   Size:        262    1061
 
SspI AAT'ATT

Cuts at:      0     238     683    1323 
   Size:        238     445     640

  Fragments arranged by size: 

                 640     445     238
 
StyI C'CwwG_G

Cuts at:      0     564    1323 
   Size:        564     759
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     730    1323 
   Size:        730     593
 
TatI w'GTAC_w

Cuts at:      0    1201    1323 
   Size:       1201     122
 
Tth111I GACn'n_nGTC

Cuts at:      0     121    1323 
   Size:        121    1202
 
XbaI T'CTAG_A

Cuts at:      0     502    1323 
   Size:        502     821
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     286    1323 
   Size:        286    1037
 

 Enzymes that do cut:

     AccI     AleI    AlwNI     ApoI     BlpI Bme1580I     BmrI     BplI
     BpmI    BpuEI    BsaAI    BsaBI    BsaWI    BseYI     BsmI    BsmBI
 Bsp1286I    BsrFI    BsrGI   BstAPI    BstYI     BtgI    BtgZI     BtsI
     EaeI  Eco57MI    HaeII    Hin4I   HincII     MscI     MslI     NaeI
     NcoI   NgoMIV     NspI    PflMI     PpiI     PsiI     SalI     SfcI
    SgrAI     SmlI     SspI     StyI    TaqII     TatI  Tth111I     XbaI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
    BmgBI     BmtI   Bpu10I     BsaI    BsaHI    BsaXI    BseRI     BsgI
    BsiEI  BsiHKAI    BsiWI    BspEI    BspHI    BspMI    BsrBI    BsrDI
   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI   MspA1I
     NarI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PfoI     PmeI     PmlI    PpuMI    PshAI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TstI     XhoI
     XmaI     XmnI     ZraI