Restriction map for
pFN21AE2511
(Linear) (Six-base) MAPSORT of: KIEE2511.seq Check: 1586 from: 1 to: 834
KIEE2511.orf
With 182 enzymes: SgfI *
February 25, 2014 15:45 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 330 834
Size: 330 504
AfeI AGC'GCT
Cuts at: 0 343 834
Size: 343 491
AlwNI CAG_nnn'CTG
Cuts at: 0 675 834
Size: 675 159
ApaI G_GGCC'C
Cuts at: 0 477 615 834
Size: 477 138 219
Fragments arranged by size:
477 219 138
BamHI G'GATC_C
Cuts at: 0 686 834
Size: 686 148
BanI G'GyrC_C
Cuts at: 0 138 314 834
Size: 138 176 520
Fragments arranged by size:
520 176 138
BanII G_rGCy'C
Cuts at: 0 477 615 834
Size: 477 138 219
Fragments arranged by size:
477 219 138
BbeI G_GCGC'C
Cuts at: 0 318 834
Size: 318 516
BlpI GC'TnA_GC
Cuts at: 0 377 834
Size: 377 457
Bme1580I G_kGCm'C
Cuts at: 0 477 615 834
Size: 477 138 219
Fragments arranged by size:
477 219 138
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 718 834
Size: 247 471 116
Fragments arranged by size:
471 247 116
BsaHI Gr'CG_yC
Cuts at: 0 315 834
Size: 315 519
BsaWI w'CCGG_w
Cuts at: 0 791 834
Size: 791 43
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 43 46 61 73 76 79 295
Size: 28 15 3 15 12 3 3 216
Cuts at: 295 403 834
Size: 108 431
Fragments arranged by size:
431 216 108 28 15 15 12 3
3 3
Bsp1286I G_dGCh'C
Cuts at: 0 477 615 834
Size: 477 138 219
Fragments arranged by size:
477 219 138
BspEI T'CCGG_A
Cuts at: 0 791 834
Size: 791 43
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 230 834
Size: 230 604
BsrBI CCG'CTC
Cuts at: 0 312 834
Size: 312 522
BsrFI r'CCGG_y
Cuts at: 0 90 317 834
Size: 90 227 517
Fragments arranged by size:
517 227 90
BsrGI T'GTAC_A
Cuts at: 0 249 761 834
Size: 249 512 73
Fragments arranged by size:
512 249 73
BstYI r'GATC_y
Cuts at: 0 161 686 834
Size: 161 525 148
Fragments arranged by size:
525 161 148
BtgI C'CryG_G
Cuts at: 0 134 834
Size: 134 700
ClaI AT'CG_AT
Cuts at: 0 245 834
Size: 245 589
EarI CTCTTCn'nnn_
Cuts at: 0 38 74 101 272 401 407 834
Size: 38 36 27 171 129 6 427
Fragments arranged by size:
427 171 129 38 36 27 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 400 834
Size: 400 434
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 330 718 834
Size: 247 83 388 116
Fragments arranged by size:
388 247 116 83
EcoNI CCTnn'n_nnAGG
Cuts at: 0 496 834
Size: 496 338
EcoO109I rG'GnC_Cy
Cuts at: 0 473 834
Size: 473 361
FspI TGC'GCA
Cuts at: 0 550 834
Size: 550 284
FspAI rTGC'GCAy
Cuts at: 0 550 834
Size: 550 284
HaeII r_GCGC'y
Cuts at: 0 318 345 834
Size: 318 27 489
Fragments arranged by size:
489 318 27
HincII GTy'rAC
Cuts at: 0 183 834
Size: 183 651
KasI G'GCGC_C
Cuts at: 0 314 834
Size: 314 520
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 488 659 798 834
Size: 488 171 139 36
Fragments arranged by size:
488 171 139 36
MspA1I CmG'CkG
Cuts at: 0 264 696 827 834
Size: 264 432 131 7
Fragments arranged by size:
432 264 131 7
NarI GG'CG_CC
Cuts at: 0 315 834
Size: 315 519
PflMI CCAn_nnn'nTGG
Cuts at: 0 696 834
Size: 696 138
PspOMI G'GGCC_C
Cuts at: 0 473 611 834
Size: 473 138 223
Fragments arranged by size:
473 223 138
PstI C_TGCA'G
Cuts at: 0 236 834
Size: 236 598
PvuII CAG'CTG
Cuts at: 0 696 834
Size: 696 138
SapI GCTCTTCn'nnn_
Cuts at: 0 74 834
Size: 74 760
SexAI A'CCwGG_T
Cuts at: 0 491 834
Size: 491 343
SfcI C'TryA_G
Cuts at: 0 232 834
Size: 232 602
SfoI GGC'GCC
Cuts at: 0 316 834
Size: 316 518
TatI w'GTAC_w
Cuts at: 0 249 761 834
Size: 249 512 73
Fragments arranged by size:
512 249 73
Enzymes that do cut:
AcuI AfeI AlwNI ApaI BamHI BanI BanII BbeI
BlpI Bme1580I BpmI BsaHI BsaWI BseRI Bsp1286I BspEI
BspMI BsrBI BsrFI BsrGI BstYI BtgI ClaI EarI
EciI Eco57MI EcoNI EcoO109I FspI FspAI HaeII HincII
KasI MmeI MspA1I NarI PflMI PspOMI PstI PvuII
SapI SexAI SfcI SfoI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI BsrDI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EcoICRI
EcoRI EcoRV FalI FseI Hin4I HindIII HpaI KpnI
MfeI MluI MscI MslI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI