Restriction map for
pFN21AE2498
(Linear) (Six-base) MAPSORT of: KIEE2498.seq Check: 7366 from: 1 to: 13164
KIEE2498.orf
With 182 enzymes: SgfI *
March 20, 2014 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 885 4257 6618 12954 13164
Size: 885 3372 2361 6336 210
Fragments arranged by size:
6336 3372 2361 885 210
AatII G_ACGT'C
Cuts at: 0 9770 13164
Size: 9770 3394
AccI GT'mk_AC
Cuts at: 0 1465 1761 3472 8564 13164
Size: 1465 296 1711 5092 4600
Fragments arranged by size:
5092 4600 1711 1465 296
Acc65I G'GTAC_C
Cuts at: 0 2944 11653 12407 13164
Size: 2944 8709 754 757
Fragments arranged by size:
8709 2944 757 754
AclI AA'CG_TT
Cuts at: 0 1005 2558 3686 13164
Size: 1005 1553 1128 9478
Fragments arranged by size:
9478 1553 1128 1005
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 1421 2030 2059 2294 2579 3002 3283
Size: 181 1240 609 29 235 285 423 281
Cuts at: 3283 3432 3821 3877 4361 4864 5584 6212 6373
Size: 149 389 56 484 503 720 628 161
Cuts at: 6373 8059 8194 8252 8389 9870 10643 11246 12266
Size: 1686 135 58 137 1481 773 603 1020
Cuts at: 12266 13164
Size: 898
Fragments arranged by size:
1686 1481 1240 1020 898 773 720 628
609 603 503 484 423 389 285 281
235 181 161 149 137 135 58 56
29
AfeI AGC'GCT
Cuts at: 0 6052 13164
Size: 6052 7112
AflII C'TTAA_G
Cuts at: 0 12025 13164
Size: 12025 1139
AflIII A'CryG_T
Cuts at: 0 1460 2538 13164
Size: 1460 1078 10626
Fragments arranged by size:
10626 1460 1078
AhdI GACnn_n'nnGTC
Cuts at: 0 8490 13164
Size: 8490 4674
AleI CACnn'nnGTG
Cuts at: 0 7483 8416 13164
Size: 7483 933 4748
Fragments arranged by size:
7483 4748 933
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1300 7501 7808 13164
Size: 1300 6201 307 5356
Fragments arranged by size:
6201 5356 1300 307
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1683 1715 10266 10298 10598 10630 13164
Size: 1683 32 8551 32 300 32 2534
Fragments arranged by size:
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AlwNI CAG_nnn'CTG
Cuts at: 0 2272 4739 7941 8083 8956 9913 11071 12714
Size: 2272 2467 3202 142 873 957 1158 1643
Cuts at: 12714 12824 13164
Size: 110 340
Fragments arranged by size:
3202 2467 2272 1643 1158 957 873 340
142 110
ApaI G_GGCC'C
Cuts at: 0 3120 4917 12707 13164
Size: 3120 1797 7790 457
Fragments arranged by size:
7790 3120 1797 457
ApaLI G'TGCA_C
Cuts at: 0 8691 9122 11575 11762 13164
Size: 8691 431 2453 187 1402
Fragments arranged by size:
8691 2453 1402 431 187
ApoI r'AATT_y
Cuts at: 0 452 604 862 1497 1629 2173 2796 2872
Size: 452 152 258 635 132 544 623 76
Cuts at: 2872 3209 3282 3717 3936 4224 4582 4957 5335
Size: 337 73 435 219 288 358 375 378
Cuts at: 5335 5392 6141 6639 6781 6815 7610 7632 8202
Size: 57 749 498 142 34 795 22 570
Cuts at: 8202 8854 9003 9693 10255 10770 12790 13164
Size: 652 149 690 562 515 2020 374
Fragments arranged by size:
2020 795 749 690 652 635 623 570
562 544 515 498 452 435 378 375
374 358 337 288 258 219 152 149
142 132 76 73 57 34 22
AseI AT'TA_AT
Cuts at: 0 2657 9608 11294 11525 12083 13164
Size: 2657 6951 1686 231 558 1081
Fragments arranged by size:
6951 2657 1686 1081 558 231
AvaI C'yCGr_G
Cuts at: 0 1582 2341 4046 5072 12101 13164
Size: 1582 759 1705 1026 7029 1063
Fragments arranged by size:
7029 1705 1582 1063 1026 759
AvrII C'CTAG_G
Cuts at: 0 6342 13164
Size: 6342 6822
BamHI G'GATC_C
Cuts at: 0 5604 7599 8529 9820 13164
Size: 5604 1995 930 1291 3344
Fragments arranged by size:
5604 3344 1995 1291 930
BanI G'GyrC_C
Cuts at: 0 2944 3598 6002 6193 6531 7385 7477 11653
Size: 2944 654 2404 191 338 854 92 4176
Cuts at: 11653 12407 12595 13164
Size: 754 188 569
Fragments arranged by size:
4176 2944 2404 854 754 654 569 338
191 188 92
BanII G_rGCy'C
Cuts at: 0 270 1260 3120 4917 5082 5312 6290 8521
Size: 270 990 1860 1797 165 230 978 2231
Cuts at: 8521 8950 10868 10981 12707 13164
Size: 429 1918 113 1726 457
Fragments arranged by size:
2231 1918 1860 1797 1726 990 978 457
429 270 230 165 113
BbeI G_GCGC'C
Cuts at: 0 6006 12599 13164
Size: 6006 6593 565
Fragments arranged by size:
6593 6006 565
BbsI GAAGACnn'nnnn_
Cuts at: 0 1704 2028 4048 5196 6446 7054 7352 8513
Size: 1704 324 2020 1148 1250 608 298 1161
Cuts at: 8513 9367 12077 12370 13164
Size: 854 2710 293 794
Fragments arranged by size:
2710 2020 1704 1250 1161 1148 854 794
608 324 298 293
BbvCI CC'TCA_GC
Cuts at: 0 11010 12172 13164
Size: 11010 1162 992
Fragments arranged by size:
11010 1162 992
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1018 1052 1430 1464 7441 7475 11017 11051
Size: 1018 34 378 34 5977 34 3542 34
Cuts at: 11051 13164
Size: 2113
Fragments arranged by size:
5977 3542 2113 1018 378 34 34 34
34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3012 4691 8334 10443 13164
Size: 3012 1679 3643 2109 2721
Fragments arranged by size:
3643 3012 2721 2109 1679
BclI T'GATC_A
Cuts at: 0 1788 5459 10350 11792 13164
Size: 1788 3671 4891 1442 1372
Fragments arranged by size:
4891 3671 1788 1442 1372
BfrBI ATG'CAT
Cuts at: 0 7669 13164
Size: 7669 5495
BglI GCCn_nnn'nGGC
Cuts at: 0 6615 8234 12711 13164
Size: 6615 1619 4477 453
Fragments arranged by size:
6615 4477 1619 453
BglII A'GATC_T
Cuts at: 0 3829 4668 4899 6552 7147 8612 9830 13164
Size: 3829 839 231 1653 595 1465 1218 3334
Fragments arranged by size:
3829 3334 1653 1465 1218 839 595 231
BlpI GC'TnA_GC
Cuts at: 0 11921 13164
Size: 11921 1243
Bme1580I G_kGCm'C
Cuts at: 0 1417 3120 3274 4917 6371 8695 9126 11579
Size: 1417 1703 154 1643 1454 2324 431 2453
Cuts at: 11579 11766 12707 13164
Size: 187 941 457
Fragments arranged by size:
2453 2324 1703 1643 1454 1417 941 457
431 187 154
BmgBI CAC'GTC
Cuts at: 0 2526 11135 13164
Size: 2526 8609 2029
Fragments arranged by size:
8609 2526 2029
BmrI ACTGGGnnnn_n'
Cuts at: 0 649 2277 3114 4305 6365 7244 8944 11747
Size: 649 1628 837 1191 2060 879 1700 2803
Cuts at: 11747 13164
Size: 1417
Fragments arranged by size:
2803 2060 1700 1628 1417 1191 879 837
649
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2466 2638 5555 5780 6613 7845 8755 9414
Size: 2466 172 2917 225 833 1232 910 659
Cuts at: 9414 10998 13164
Size: 1584 2166
Fragments arranged by size:
2917 2466 2166 1584 1232 910 833 659
225 172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 231 370 736 832 1223 1282 2226 2405
Size: 231 139 366 96 391 59 944 179
Cuts at: 2405 2455 4510 4773 6463 6907 7411 7720 7840
Size: 50 2055 263 1690 444 504 309 120
Cuts at: 7840 8279 9197 9709 10421 11350 13164
Size: 439 918 512 712 929 1814
Fragments arranged by size:
2055 1814 1690 944 929 918 712 512
504 444 439 391 366 309 263 231
179 139 120 96 59 50
Bpu10I CC'TnA_GC
Cuts at: 0 5084 11010 11689 12172 12357 13164
Size: 5084 5926 679 483 185 807
Fragments arranged by size:
5926 5084 807 679 483 185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 853 2014 2917 3096 5614 9193 11824 12650
Size: 853 1161 903 179 2518 3579 2631 826
Cuts at: 12650 13164
Size: 514
Fragments arranged by size:
3579 2631 2518 1161 903 853 826 514
179
BsaI GGTCTCn'nnnn_
Cuts at: 0 779 808 1667 2272 5174 5723 11062 11301
Size: 779 29 859 605 2902 549 5339 239
Cuts at: 11301 11707 12112 13164
Size: 406 405 1052
Fragments arranged by size:
5339 2902 1052 859 779 605 549 406
405 239 29
BsaAI yAC'GTr
Cuts at: 0 1463 10505 12876 13164
Size: 1463 9042 2371 288
Fragments arranged by size:
9042 2371 1463 288
BsaBI GATnn'nnATC
Cuts at: 0 241 4216 10009 13164
Size: 241 3975 5793 3155
Fragments arranged by size:
5793 3975 3155 241
BsaHI Gr'CG_yC
Cuts at: 0 6003 9767 12596 13164
Size: 6003 3764 2829 568
Fragments arranged by size:
6003 3764 2829 568
BsaWI w'CCGG_w
Cuts at: 0 1649 5420 5876 5906 8900 9143 9797 10616
Size: 1649 3771 456 30 2994 243 654 819
Cuts at: 10616 11387 13164
Size: 771 1777
Fragments arranged by size:
3771 2994 1777 1649 819 771 654 456
243 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 295 325 709 739 1102 1132 1213 1243
Size: 295 30 384 30 363 30 81 30
Cuts at: 1243 2238 2268 2322 2352 3895 3925 5271 5301
Size: 995 30 54 30 1543 30 1346 30
Cuts at: 5301 6050 6080 7693 7723 8537 8567 10598 10628
Size: 749 30 1613 30 814 30 2031 30
Cuts at: 10628 11213 11243 12860 12890 13164
Size: 585 30 1617 30 274
Fragments arranged by size:
2031 1617 1613 1543 1346 995 814 749
585 384 363 295 274 81 54 30
30 30 30 30 30 30 30 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 367 1435 1541 2119 2689 2776 2851 4689
Size: 367 1068 106 578 570 87 75 1838
Cuts at: 4689 4709 5090 6298 6687 8351 9943 10511 10532
Size: 20 381 1208 389 1664 1592 568 21
Cuts at: 10532 10971 11827 11839 12160 12828 13164
Size: 439 856 12 321 668 336
Fragments arranged by size:
1838 1664 1592 1208 1068 856 668 578
570 568 439 389 381 367 336 321
106 87 75 21 20 12
BseYI C'CCAG_C
Cuts at: 0 1797 6047 6919 7039 7941 8288 8933 9675
Size: 1797 4250 872 120 902 347 645 742
Cuts at: 9675 11721 13164
Size: 2046 1443
Fragments arranged by size:
4250 2046 1797 1443 902 872 742 645
347 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2660 5539 5941 8720 9984 12681 12982 13164
Size: 2660 2879 402 2779 1264 2697 301 182
Fragments arranged by size:
2879 2779 2697 2660 1264 402 301 182
BsiEI CG_ry'CG
Cuts at: 0 3180 13164
Size: 3180 9984
BsiHKAI G_wGCw'C
Cuts at: 0 2431 2624 2933 6090 7490 8280 8609 8695
Size: 2431 193 309 3157 1400 790 329 86
Cuts at: 8695 9126 10868 11364 11579 11766 12833 12988 13164
Size: 431 1742 496 215 187 1067 155 176
Fragments arranged by size:
3157 2431 1742 1400 1067 790 496 431
329 309 215 193 187 176 155 86
BsmI GAATG_Cn'
Cuts at: 0 975 2219 5004 5549 7669 8031 9605 9758
Size: 975 1244 2785 545 2120 362 1574 153
Cuts at: 9758 10967 11972 13164
Size: 1209 1005 1192
Fragments arranged by size:
2785 2120 1574 1244 1209 1192 1005 975
545 362 153
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3343 4106 8356 13164
Size: 3343 763 4250 4808
Fragments arranged by size:
4808 4250 3343 763
Bsp1286I G_dGCh'C
Cuts at: 0 270 1260 1417 2431 2624 2933 3120 3274
Size: 270 990 157 1014 193 309 187 154
Cuts at: 3274 4917 5082 5312 6090 6290 6371 7490 8280
Size: 1643 165 230 778 200 81 1119 790
Cuts at: 8280 8521 8609 8695 8950 9126 10868 10981 11364
Size: 241 88 86 255 176 1742 113 383
Cuts at: 11364 11579 11766 12707 12833 12988 13164
Size: 215 187 941 126 155 176
Fragments arranged by size:
1742 1643 1119 1014 990 941 790 778
383 309 270 255 241 230 215 200
193 187 187 176 176 165 157 155
154 126 113 88 86 81
BspEI T'CCGG_A
Cuts at: 0 5876 5906 8900 9797 10616 13164
Size: 5876 30 2994 897 819 2548
Fragments arranged by size:
5876 2994 2548 897 819 30
BspHI T'CATG_A
Cuts at: 0 1176 4565 13164
Size: 1176 3389 8599
Fragments arranged by size:
8599 3389 1176
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 885 4257 4309 4620 6618 10189 12954 13164
Size: 885 3372 52 311 1998 3571 2765 210
Fragments arranged by size:
3571 3372 2765 1998 885 311 210 52
BsrBI CCG'CTC
Cuts at: 0 11021 12393 13164
Size: 11021 1372 771
Fragments arranged by size:
11021 1372 771
BsrDI GCAATG_nn'
Cuts at: 0 2916 5534 7763 10781 11700 13164
Size: 2916 2618 2229 3018 919 1464
Fragments arranged by size:
3018 2916 2618 2229 1464 919
BsrFI r'CCGG_y
Cuts at: 0 848 2915 8648 11716 12442 13164
Size: 848 2067 5733 3068 726 722
Fragments arranged by size:
5733 3068 2067 848 726 722
BsrGI T'GTAC_A
Cuts at: 0 564 4658 6842 11628 13164
Size: 564 4094 2184 4786 1536
Fragments arranged by size:
4786 4094 2184 1536 564
BssSI C'ACGA_G
Cuts at: 0 1037 1449 4654 12987 13164
Size: 1037 412 3205 8333 177
Fragments arranged by size:
8333 3205 1037 412 177
BstAPI GCAn_nnn'nTGC
Cuts at: 0 5010 5928 13164
Size: 5010 918 7236
Fragments arranged by size:
7236 5010 918
BstEII G'GTnAC_C
Cuts at: 0 12966 13164
Size: 12966 198
BstXI CCAn_nnnn'nTGG
Cuts at: 0 687 6999 9312 12588 13164
Size: 687 6312 2313 3276 576
Fragments arranged by size:
6312 3276 2313 687 576
BstYI r'GATC_y
Cuts at: 0 1406 1806 2202 2598 3829 4496 4668 4899
Size: 1406 400 396 396 1231 667 172 231
Cuts at: 4899 5604 5625 6474 6552 6717 6807 6897 7147
Size: 705 21 849 78 165 90 90 250
Cuts at: 7147 7599 7902 8529 8612 9820 9830 10511 12021
Size: 452 303 627 83 1208 10 681 1510
Cuts at: 12021 12090 13164
Size: 69 1074
Fragments arranged by size:
1510 1406 1231 1208 1074 849 705 681
667 627 452 400 396 396 303 250
231 172 165 90 90 83 78 69
21 10
BstZ17I GTA'TAC
Cuts at: 0 1466 13164
Size: 1466 11698
Bsu36I CC'TnA_GG
Cuts at: 0 1968 2147 5543 13164
Size: 1968 179 3396 7621
Fragments arranged by size:
7621 3396 1968 179
BtgI C'CryG_G
Cuts at: 0 674 3917 5585 5784 6684 6741 11252 12450
Size: 674 3243 1668 199 900 57 4511 1198
Cuts at: 12450 12945 13031 13164
Size: 495 86 133
Fragments arranged by size:
4511 3243 1668 1198 900 674 495 199
133 86 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 12783 13164
Size: 12783 381
BtsI GCAGTG_nn'
Cuts at: 0 1284 1396 1436 3506 4386 5464 5510 6705
Size: 1284 112 40 2070 880 1078 46 1195
Cuts at: 6705 7805 9110 9126 11072 11747 12924 12999 13164
Size: 1100 1305 16 1946 675 1177 75 165
Fragments arranged by size:
2070 1946 1305 1284 1195 1177 1100 1078
880 675 165 112 75 46 40 16
ClaI AT'CG_AT
Cuts at: 0 1182 3277 8147 13164
Size: 1182 2095 4870 5017
Fragments arranged by size:
5017 4870 2095 1182
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 413 448 2522 2557 13164
Size: 413 35 2074 35 10607
Fragments arranged by size:
10607 2074 413 35 35
DraI TTT'AAA
Cuts at: 0 2877 5850 10907 13164
Size: 2877 2973 5057 2257
Fragments arranged by size:
5057 2973 2877 2257
DraIII CAC_nnn'GTG
Cuts at: 0 2961 5562 8690 8699 13164
Size: 2961 2601 3128 9 4465
Fragments arranged by size:
4465 3128 2961 2601 9
EaeI y'GGCC_r
Cuts at: 0 677 2315 2913 6417 8311 13164
Size: 677 1638 598 3504 1894 4853
Fragments arranged by size:
4853 3504 1894 1638 677 598
EarI CTCTTCn'nnn_
Cuts at: 0 739 1124 1397 1622 1709 2270 2959 3302
Size: 739 385 273 225 87 561 689 343
Cuts at: 3302 5403 6349 7330 8526 8834 9078 12766 13164
Size: 2101 946 981 1196 308 244 3688 398
Fragments arranged by size:
3688 2101 1196 981 946 739 689 561
398 385 343 308 273 244 225 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2884 12757 13164
Size: 2884 9873 407
Fragments arranged by size:
9873 2884 407
EcoICRI GAG'CTC
Cuts at: 0 10866 13164
Size: 10866 2298
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 231 370 736 832 1223 1282 1421
Size: 181 50 139 366 96 391 59 139
Cuts at: 1421 2030 2059 2226 2294 2405 2455 2579 3002
Size: 609 29 167 68 111 50 124 423
Cuts at: 3002 3283 3432 3821 3877 4361 4510 4773 4864
Size: 281 149 389 56 484 149 263 91
Cuts at: 4864 5584 6212 6373 6463 6907 7411 7720 7840
Size: 720 628 161 90 444 504 309 120
Cuts at: 7840 8059 8194 8252 8279 8389 9197 9709 9870
Size: 219 135 58 27 110 808 512 161
Cuts at: 9870 10421 10643 11246 11350 12266 13164
Size: 551 222 603 104 916 898
Fragments arranged by size:
916 898 808 720 628 609 603 551
512 504 484 444 423 391 389 366
309 281 263 222 219 181 167 161
161 149 149 139 139 135 124 120
111 110 104 96 91 90 68 59
58 56 50 50 29 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 12175 13164
Size: 12175 989
EcoO109I rG'GnC_Cy
Cuts at: 0 1892 3116 4913 6923 8972 10572 10709 12030
Size: 1892 1224 1797 2010 2049 1600 137 1321
Cuts at: 12030 12285 12703 12704 13053 13164
Size: 255 418 1 349 111
Fragments arranged by size:
2049 2010 1892 1797 1600 1321 1224 418
349 255 137 111 1
EcoRI G'AATT_C
Cuts at: 0 862 3717 4957 5392 6815 7632 12790 13164
Size: 862 2855 1240 435 1423 817 5158 374
Fragments arranged by size:
5158 2855 1423 1240 862 817 435 374
EcoRV GAT'ATC
Cuts at: 0 932 7725 8403 12144 13164
Size: 932 6793 678 3741 1020
Fragments arranged by size:
6793 3741 1020 932 678
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 211 355 2425 2706 3250 4143 5131 11218
Size: 211 144 2070 281 544 893 988 6087
Cuts at: 11218 11251 13164
Size: 33 1913
Fragments arranged by size:
6087 2070 1913 988 893 544 281 211
144 33
HaeII r_GCGC'y
Cuts at: 0 6006 6054 8095 8649 12599 13164
Size: 6006 48 2041 554 3950 565
Fragments arranged by size:
6006 3950 2041 565 554 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 61 93 1033 1065 1354 1386 1466 1498
Size: 61 32 940 32 289 32 80 32
Cuts at: 1498 2656 2688 3214 3246 3259 3291 4003 4035
Size: 1158 32 526 32 13 32 712 32
Cuts at: 4035 4231 4263 4498 4515 4530 4547 5055 5087
Size: 196 32 235 17 15 17 508 32
Cuts at: 5087 5271 5303 6202 6234 6542 6574 7859 7891
Size: 184 32 899 32 308 32 1285 32
Cuts at: 7891 8476 8508 8537 8569 9190 9222 9792 9824
Size: 585 32 29 32 621 32 570 32
Cuts at: 9824 10207 10239 11784 11816 12360 12392 12595 12627
Size: 383 32 1545 32 544 32 203 32
Cuts at: 12627 12989 13021 13164
Size: 362 32 143
Fragments arranged by size:
1545 1285 1158 940 899 712 621 585
570 544 526 508 383 362 308 289
235 203 196 184 143 80 61 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 29 17
17 15 13
HincII GTy'rAC
Cuts at: 0 3908 5418 9771 10416 11736 13164
Size: 3908 1510 4353 645 1320 1428
Fragments arranged by size:
4353 3908 1510 1428 1320 645
HindIII A'AGCT_T
Cuts at: 0 3444 3803 4448 10234 13164
Size: 3444 359 645 5786 2930
Fragments arranged by size:
5786 3444 2930 645 359
HpaI GTT'AAC
Cuts at: 0 5418 13164
Size: 5418 7746
KasI G'GCGC_C
Cuts at: 0 6002 12595 13164
Size: 6002 6593 569
Fragments arranged by size:
6593 6002 569
KpnI G_GTAC'C
Cuts at: 0 2948 11657 12411 13164
Size: 2948 8709 754 753
Fragments arranged by size:
8709 2948 754 753
MfeI C'AATT_G
Cuts at: 0 2499 7592 7993 10386 13164
Size: 2499 5093 401 2393 2778
Fragments arranged by size:
5093 2778 2499 2393 401
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 1102 2463 2801 3115 3224 5304 5320
Size: 293 809 1361 338 314 109 2080 16
Cuts at: 5320 5549 7001 7845 10865 12029 13164
Size: 229 1452 844 3020 1164 1135
Fragments arranged by size:
3020 2080 1452 1361 1164 1135 844 809
338 314 293 229 109 16
MscI TGG'CCA
Cuts at: 0 679 2317 6419 8313 13164
Size: 679 1638 4102 1894 4851
Fragments arranged by size:
4851 4102 1894 1638 679
MslI CAynn'nnrTG
Cuts at: 0 2434 2657 2926 4622 5896 6850 6868 7483
Size: 2434 223 269 1696 1274 954 18 615
Cuts at: 7483 7546 8416 8891 11251 12113 12802 13164
Size: 63 870 475 2360 862 689 362
Fragments arranged by size:
2434 2360 1696 1274 954 870 862 689
615 475 362 269 223 63 18
MspA1I CmG'CkG
Cuts at: 0 810 840 1801 2769 5985 6743 7079 7752
Size: 810 30 961 968 3216 758 336 673
Cuts at: 7752 7923 9652 9783 10938 11589 11725 11920 12536
Size: 171 1729 131 1155 651 136 195 616
Cuts at: 12536 12653 13149 13164
Size: 117 496 15
Fragments arranged by size:
3216 1729 1155 968 961 810 758 673
651 616 496 336 195 171 136 131
117 30 15
NaeI GCC'GGC
Cuts at: 0 8650 12444 13164
Size: 8650 3794 720
Fragments arranged by size:
8650 3794 720
NarI GG'CG_CC
Cuts at: 0 6003 12596 13164
Size: 6003 6593 568
Fragments arranged by size:
6593 6003 568
NcoI C'CATG_G
Cuts at: 0 674 3917 5585 5784 6684 11252 12450 12945
Size: 674 3243 1668 199 900 4568 1198 495
Cuts at: 12945 13031 13164
Size: 86 133
Fragments arranged by size:
4568 3243 1668 1198 900 674 495 199
133 86
NdeI CA'TA_TG
Cuts at: 0 3033 3477 11406 13164
Size: 3033 444 7929 1758
Fragments arranged by size:
7929 3033 1758 444
NgoMIV G'CCGG_C
Cuts at: 0 8648 12442 13164
Size: 8648 3794 722
Fragments arranged by size:
8648 3794 722
NsiI A_TGCA'T
Cuts at: 0 7671 13164
Size: 7671 5493
NspI r_CATG'y
Cuts at: 0 2512 2542 4621 5581 5783 10041 11607 13164
Size: 2512 30 2079 960 202 4258 1566 1557
Fragments arranged by size:
4258 2512 2079 1566 1557 960 202 30
PacI TTA_AT'TAA
Cuts at: 0 11525 13164
Size: 11525 1639
PasI CC'CwG_GG
Cuts at: 0 6290 6306 8077 10120 12708 13164
Size: 6290 16 1771 2043 2588 456
Fragments arranged by size:
6290 2588 2043 1771 456 16
PciI A'CATG_T
Cuts at: 0 2538 13164
Size: 2538 10626
PflMI CCAn_nnn'nTGG
Cuts at: 0 2478 6486 8542 9344 9402 9610 12456 13164
Size: 2478 4008 2056 802 58 208 2846 708
Fragments arranged by size:
4008 2846 2478 2056 802 708 208 58
PfoI T'CCnGG_A
Cuts at: 0 713 1239 1251 1311 2242 2513 2610 5236
Size: 713 526 12 60 931 271 97 2626
Cuts at: 5236 6068 6479 7697 10742 12158 13164
Size: 832 411 1218 3045 1416 1006
Fragments arranged by size:
3045 2626 1416 1218 1006 931 832 713
526 411 271 97 60 12
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 10598 10630 11298 11330 13164
Size: 10598 32 668 32 1834
Fragments arranged by size:
10598 1834 668 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 6923 8972 10572 10709 13053 13164
Size: 6923 2049 1600 137 2344 111
Fragments arranged by size:
6923 2344 2049 1600 137 111
PsiI TTA'TAA
Cuts at: 0 3878 8270 12493 13164
Size: 3878 4392 4223 671
Fragments arranged by size:
4392 4223 3878 671
PspOMI G'GGCC_C
Cuts at: 0 3116 4913 12703 13164
Size: 3116 1797 7790 461
Fragments arranged by size:
7790 3116 1797 461
PspXI vC'TCGA_Gb
Cuts at: 0 1582 13164
Size: 1582 11582
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 8 40 1891 1923 8565 8597 11603 11635
Size: 8 32 1851 32 6642 32 3006 32
Cuts at: 11635 13164
Size: 1529
Fragments arranged by size:
6642 3006 1851 1529 32 32 32 32
8
PstI C_TGCA'G
Cuts at: 0 1292 1443 1779 1806 4268 5517 5990 6504
Size: 1292 151 336 27 2462 1249 473 514
Cuts at: 6504 7800 7937 9369 9918 11067 11447 13164
Size: 1296 137 1432 549 1149 380 1717
Fragments arranged by size:
2462 1717 1432 1296 1292 1249 1149 549
514 473 380 336 151 137 27
PvuII CAG'CTG
Cuts at: 0 810 840 1801 5985 7079 7752 7923 9652
Size: 810 30 961 4184 1094 673 171 1729
Cuts at: 9652 9783 10938 11589 11725 11920 13149 13164
Size: 131 1155 651 136 195 1229 15
Fragments arranged by size:
4184 1729 1229 1155 1094 961 810 673
651 195 171 136 131 30 15
SacI G_AGCT'C
Cuts at: 0 10868 13164
Size: 10868 2296
SacII CC_GC'GG
Cuts at: 0 6744 13164
Size: 6744 6420
SanDI GG'GwC_CC
Cuts at: 0 6923 13164
Size: 6923 6241
SapI GCTCTTCn'nnn_
Cuts at: 0 8526 12766 13164
Size: 8526 4240 398
Fragments arranged by size:
8526 4240 398
ScaI AGT'ACT
Cuts at: 0 3294 3983 13164
Size: 3294 689 9181
Fragments arranged by size:
9181 3294 689
SexAI A'CCwGG_T
Cuts at: 0 11310 13164
Size: 11310 1854
SfcI C'TryA_G
Cuts at: 0 1288 1439 1682 1775 1802 2566 3313 3722
Size: 1288 151 243 93 27 764 747 409
Cuts at: 3722 4264 4502 5513 5796 5986 6500 7316 7796
Size: 542 238 1011 283 190 514 816 480
Cuts at: 7796 7933 9292 9365 9914 11063 11443 13164
Size: 137 1359 73 549 1149 380 1721
Fragments arranged by size:
1721 1359 1288 1149 1011 816 764 747
549 542 514 480 409 380 283 243
238 190 151 137 93 73 27
SfoI GGC'GCC
Cuts at: 0 6004 12597 13164
Size: 6004 6593 567
Fragments arranged by size:
6593 6004 567
SmlI C'TyrA_G
Cuts at: 0 868 1582 1993 2932 3075 5072 5629 9172
Size: 868 714 411 939 143 1997 557 3543
Cuts at: 9172 11839 12025 12665 13164
Size: 2667 186 640 499
Fragments arranged by size:
3543 2667 1997 939 868 714 640 557
499 411 186 143
SnaBI TAC'GTA
Cuts at: 0 10505 13164
Size: 10505 2659
SpeI A'CTAG_T
Cuts at: 0 3190 13164
Size: 3190 9974
SphI G_CATG'C
Cuts at: 0 10041 13164
Size: 10041 3123
SspI AAT'ATT
Cuts at: 0 441 1066 9048 13164
Size: 441 625 7982 4116
Fragments arranged by size:
7982 4116 625 441
StuI AGG'CCT
Cuts at: 0 11 1088 2970 10830 13164
Size: 11 1077 1882 7860 2334
Fragments arranged by size:
7860 2334 1882 1077 11
StyI C'CwwG_G
Cuts at: 0 229 674 920 2051 3392 3917 4036 4917
Size: 229 445 246 1131 1341 525 119 881
Cuts at: 4917 5585 5784 6342 6684 7116 7154 8051 8308
Size: 668 199 558 342 432 38 897 257
Cuts at: 8308 8381 8444 10454 11252 12044 12122 12450 12552
Size: 73 63 2010 798 792 78 328 102
Cuts at: 12552 12945 13031 13164
Size: 393 86 133
Fragments arranged by size:
2010 1341 1131 897 881 798 792 668
558 525 445 432 393 342 328 257
246 229 199 133 119 102 86 78
73 63 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 222 1645 2080 2245 3153 3194 4324 5156
Size: 222 1423 435 165 908 41 1130 832
Cuts at: 5156 7129 8739 8905 9304 10899 13164
Size: 1973 1610 166 399 1595 2265
Fragments arranged by size:
2265 1973 1610 1595 1423 1130 908 832
435 399 222 166 165 41
TatI w'GTAC_w
Cuts at: 0 564 3292 3981 4658 6842 8581 11628 12398
Size: 564 2728 689 677 2184 1739 3047 770
Cuts at: 12398 13164
Size: 766
Fragments arranged by size:
3047 2728 2184 1739 770 766 689 677
564
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1102 1134 2322 2354 3128 3160 5822 5854
Size: 1102 32 1188 32 774 32 2662 32
Cuts at: 5854 6050 6082 7830 7862 7887 7919 11558 11590
Size: 196 32 1748 32 25 32 3639 32
Cuts at: 11590 12860 12892 13164
Size: 1270 32 272
Fragments arranged by size:
3639 2662 1748 1270 1188 1102 774 272
196 32 32 32 32 32 32 32
32 32 25
Tth111I GACn'n_nGTC
Cuts at: 0 6445 8965 13164
Size: 6445 2520 4199
Fragments arranged by size:
6445 4199 2520
XbaI T'CTAG_A
Cuts at: 0 12087 13164
Size: 12087 1077
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2479 3157 3924 8394 10641 11259 13164
Size: 2479 678 767 4470 2247 618 1905
Fragments arranged by size:
4470 2479 2247 1905 767 678 618
XhoI C'TCGA_G
Cuts at: 0 1582 5072 13164
Size: 1582 3490 8092
Fragments arranged by size:
8092 3490 1582
XmnI GAAnn'nnTTC
Cuts at: 0 1172 1629 2408 3938 6300 7072 8843 10738
Size: 1172 457 779 1530 2362 772 1771 1895
Cuts at: 10738 10929 11231 13164
Size: 191 302 1933
Fragments arranged by size:
2362 1933 1895 1771 1530 1172 779 772
457 302 191
ZraI GAC'GTC
Cuts at: 0 9768 13164
Size: 9768 3396
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PacI PasI PciI PflMI PfoI PpiI PpuMI PsiI
PspOMI PspXI PsrI PstI PvuII SacI SacII SanDI
SapI ScaI SexAI SfcI SfoI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AgeI AscI AsiSI BaeI BmtI BsiWI BssHII BstBI
DrdI EagI FseI FspI FspAI MluI NheI NotI
NruI PmeI PmlI PshAI PvuI RsrII SalI SbfI
SfiI SgfI SgrAI SmaI SrfI SwaI XmaI